Development of Environmental DNA Analysis Techniques
We are developing and improving methods to efficiently collect and accurately analyze DNA from organisms present in the environment. Specifically, this includes optimizing filtration and preservation methods, DNA extraction processes, and standardizing analytical techniques. In addition, we are working on innovative approaches that go beyond mapping species distributions by examining factors such as the ratio of nuclear DNA to mitochondrial DNA and DNA methylation patterns. These advancements aim to enable analysis of not only the presence of organisms but also their behaviors and physiological states.
Selected Publications
- Hirayama, I. T., Kunimasa, Y., Takeuchi, A., Minamoto, T. (2025) Significant improvement of environmental DNA assay by targeting retrotransposon sequences characteristic to Anguilla eels. Environmental DNA 7, e70197. LINK
- Hirayama, I. T., Wu, L., Minamoto, T. (2024) Stability of environmental DNA methylation and its utility in tracing spawning in fish. Molecular Ecology Resources 24, e14011. LINK
- Wu, L., Wu, Q., Inagawa, T., Okitsu, J., Sakamoto, S., Minamoto, T. (2023) Estimating the spawning activity of fish species using nuclear and mitochondrial environmental DNA concentrations and their ratios. Freshwater Biology 68, 103-114. LINK
- Minamoto, T., Miya, M., Sado, T., Seino, S., Doi, H., Kondoh, M., Nakamura, K., Takahara, T., Yamamoto, S., Yamanaka, H., Araki, H., Iwasaki, W., Kasai, A., Masuda, R., Uchii, K. (2021) An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols. Environmental DNA 3, 8-13. LINK
- Sakata, M. K., Yamamoto, S., Gotoh, R. O., Miya, M., Yamanaka, H., Minamoto, T. (2020) Sedimentary eDNA provides different information on timescale and fish species composition compared with aqueous eDNA. Environmental DNA, 2, 505-518. LINK
- Jo, T., Murakami, H., Masuda, R., Sakata, M. K., Yamamoto, S., Minamoto, T. (2017) Rapid degradation of longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA. Molecular Ecology Resources 17 (6), e25-e33. LINK
Monitoring of Rare and Invasive Species
We aims to detect endangered species and invasive species at an early stage and link the findings to conservation and management efforts. We develop species-specific detection methods using environmental DNA to efficiently identify a wide range of taxa, including fish and amphibians. Ultimately, our goal is to implement these techniques in policy and society.
Selected Publications
- Hashimoto, N., Iwata, T., Kihara, N., Nakamura, N., Sakata, M. K., Minamoto, T. (2024) Detection of environmental DNA of finless porpoise (Neophocaena asiaeorientalis) in Osaka Bay, Japan. Conservation Genetics Resources 16, 255-261 LINK
- Hidaka, S., Jo, T. S., Yamamoto, S., Katsuhara, K. R., Tomita, S., Miya, M., Ikegami,M., Ushimaru, A., Minamoto, T. (2024) Sensitive and efficient surveillance of Japanese giant salamander (Andrias japonicus) distribution in western Japan using multi-copy nuclear DNA marker. Limnology 25, 189-198. LINK
- Wu, Q., Zhou, J., Komoto, T., Ishikawa, T., Goto, N., Sakata, M. K., Kitazawa, D., Minamoto, T. (2023) Opposite trends in environmental DNA distributions of two freshwater species under climate change. Ecosphere 14, e4651. LINK
- Jo, T., Fukuoka, A., Uchida, K., Ushimaru A., Minamoto, T. (2020) Multiplex real-time PCR enables the simultaneous detection of environmental DNA from freshwater fishes: a case study of three exotic and three threatened native fishes in Japan. Biological Invasions 22 (2), 455-471. LINK
- Sakata, M. K., Maki, N., Sugiyama, H., Minamoto, T. (2017) Identifying a breeding habitat of a critically endangered fish, Acheilognathus typus, in a natural river in Japan. The Science of Nature - Naturwissenschaften 104, 100. LINK
Biodeversity Monitoring
We monitor the species composition and abundance of aquatic organisms, such as fish, inhabiting rivers, lakes, and coastal areas to assess the current state of ecosystems and track changes over time. By applying environmental DNA metabarcoding and quantitative eDNA analysis, we contribute to both short-term and long-term ecosystem monitoring.
Selected Publications
- Wu, Q., Nakano, T., Ishida, S., Komai, T., Fujiwara, Y., Yoshida, T., Kawato, M., Oka, S., Fujikura, K., Miya, M., Minamoto, T. (2025) Development of universal PCR primers for the environmental DNA metabarcoding of cephalopod (Mollusca) diversity. Marine Environmental Research 208, 107094. LINK
- Oyabu, A., Wu, L., Matsumoto, T., Kihara, N., Yamanaka, H., Minamoto, T. (2024) The effect of artificial light at night on wild fish community: manipulative field experiment and species composition analysis using environmental DNA. Environmental Advances 15, 100457 LINK
- Wu, L., Osugi, T., Inagawa, T., Okitsu, J., Sakamoto, S., Minamoto, T. (2024) Monitoring of multiple fish species by quantitative environmental DNA metabarcoding surveys over two summer seasons. Molecular Ecology Resources 24, e13875. LINK
- Sakata, M. K., Kawata, M. U., Kurabayashi, A., Kurita, T., Nakamura, M., Shirako, T., Kakehashi R., Nishikawa, K., Hossman, M. Y., Nishijima, T., Kawamoto, J., Miya, M., Minamoto, T. (2022) Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia. Metabarcoding and Metagenomics 6, 15-26. LINK
- Hayami, K., Sakata, M. K., Inagawa, T., Okitsu, J., Katano, I., Doi, H., Nakai, K., Ichiyanagi, H., Gotoh, R. O., Miya, M., Sato, H., Yamanaka, H., Minamoto, T. (2020) Effects of sampling seasons and locations on fish environmental DNA metabarcoding in dam reservoirs. Ecology and Evolution 10 (12), 5354-5367. LINK
Ecology of Infectious Diseases
We investigate how pathogens are distributed in aquatic environments. By detecting pathogens using environmental DNA, we assess public health risks and contribute to the prevention and management of infectious diseases. Another important research focus is the inflow and spread of antimicrobial-resistant bacteria in water systems.
Selected Publications
- Matsuo, R., Togetani, A., Adisakwattana, P., Yoonuan, T., Phuphisut, O., Limpanont, Y. Sakata, M. K., Sato, M. O., Sato, M., Minamoto, T. (2024) Improved environmental DNA detection sensitivity of Opisthorchis viverrini using a multi-marker assay. Parasitology Research 123, 419. LINK
- Osawa, R., Jo, T. S., Nakamura, R., Futami, K., Itayama, T., Chadeka, E. A., Ngetich, B., Nagi, S., Kikuchi, M., Njenga, S., Ouma, C., Sonye, G. O., Hamano, S., Minamoto, T. (2024) Methodological assessment for efficient collection of Schistosoma mansoni environmental DNA and improved schistosomiasis surveillance in tropical wetlands. Acta Tropica 260, 107402. LINK
- Hashizume, H., Sato, M., Sato, M. O., Ikeda, S., Yoounan, T., Sanguankiat, S., Pongvongsa, T., Moji, K., Minamoto, T. (2017) Application of environmental DNA analysis for the detection of Opisthorchis viverrini DNA in water samples. Acta Tropica 169, 1-7. LINK
- Minamoto, T., Pu, X., Xie, J., Dong, Y., Wu, D., Kong, H., Yang, X., Takahara, T., Honjo, M. N., Yamanaka, H., Kawabata, Z. (2015) Monitoring of fish pathogenic viruses in natural lakes in Yunnan, China. Limnology 16(1), 69-77. LINK
Urban Ecosystems and Well-being
We study how urban green spaces and blue spaces, such as parks and water bodies, benefit people’s lives and well-being. Our research analyzes the relationship between ecosystem services and human happiness, proposes policies to reduce disparities in access to nature experiences, and promotes initiatives for Satoyama restoration.
Selected Publications
- Uchiyama, Y., Kyan, A., Sato, M., Ushimaru, A., Minamoto, T., Kiyono, M., Harada, K., Takakura, M. (2024) Local environment perceived in daily life and urban green and blue space visits: Uncovering key factors for different age groups to access ecosystem services. Journal of Environmental Management 370, 122676. LINK
- Jo, T.*, Sato, M.*, Minamoto, T.*, Ushimaru A.* (2022) Valuing the cultural services from urban blue space ecosystems in Japanese megacities during the COVID-19 pandemic. People and Nature 4, 1176-1189. (*equal contribution) LINK
- Sato, M., Ushimaru, A., Minamoto, T. (2017) The effect of different personal histories on valuation of forest ecosystem services in urban areas: a case study of Mt. Rokko, Kobe, Japan. Urban Forestry & Urban Greening 28, 110-117. LINK
Physiological Ecology of Animals
We study fish, ascidians, and insects to uncover the mechanisms of genes and proteins involved in vision and the circadian clock. By clarifying the molecular structures of opsins functioning in the retina, as well as expression patterns of clock genes, we aim to understand how organisms adapt to fluctuating environmental conditions.
Selected Publications
- Minamoto, T., Hanai, S., Kadota, K., Oishi, K., Matsumae, H., Fujie, M., Azumi, K., Satoh,N., Satake, M., Ishida, N. (2010) Circadian clock in Ciona intestinalis revealed by microarray analysis and oxygen consumption. Journal of Biochemistry 147, 175–184. LINK
- 源利文, 清水勇 (2005) 魚類の視覚適応の分子的基盤 魚類学雑誌 52, 91–106 LINK
- Minamoto, T., Shimizu, I. (2005) Molecular cloning of cone opsin genes and their expression in the retina of a smelt, Ayu (Plecoglossus altivelis, Teleostei). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 140, 197–205. LINK
- Minamoto, T., Shimizu, I. (2003) Molecular cloning and characterization of rhodopsin in a teleost (Plecoglossus altivelis, Osmeridae). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 134, 559–570. LINK