メンバー紹介

特命助教佐々木 大介 Daisuke Sasaki

経歴

2008/03
東京大学大学院 農学生命科学研究科 応用生命工学専攻 博士課程修了 博士(農学)
2008/04
東京大学大学院 農学生命科学研究科 応用生命工学専攻 特任研究員
2010/04
一般財団法人電力中央研究所 バイオテクノロジー領域 特別契約研究員
2012/12
神戸大学 大学院工学研究科 応用化学専攻 特命助教
2015/04
神戸大学 自然科学系先端融合研究環 特命助教
2016/04
神戸大学大学院 科学技術イノベーション研究科 特命助教
2017/10
高機能遺伝子デザイン技術研究組合 研究員
2018/04
神戸大学大学院 科学技術イノベーション研究科 特命助教
2018/07
神戸大学 先端バイオ工学研究センター 兼任

発表論文

  1. Inokuma, K., Matsuda M., Sasaki, D., Hasunuma, T., Kondo, A. (2018) Widespread effect of N-acetyl-D-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis, Microbial Cell Factories, 17, 153
  2. Kobayashi, J., Sasaki, D., Kondo, A. (2018) A procedure for precise determination of glutathione produced by Saccharomyces cerevisiae, Bio-protocol, 8(12): e2887
  3. Sasaki, K., Sasaki, D., Tsuge, Y., Morita, M., Kondo, A. (2018) Changes in the microbial consortium during dark hydrogen fermentation in a bioelectrochemical system increases methane production during a two-stage process, Biotechnology for Biofuels, 11: 173
  4. Sasaki, K., Sasaki, D., Kamiya, K., Nakanishi, S., Kondo, A., Kato, S. (2018) Electrochemical biotechnologies minimizing the required electrode assemblies, Current Opinion in Biotechnology, 50, 182-188
  5. Sasaki, D., Sasaki, K., Ikuta, N., Yasuda, T., Fukuda, I., Kondo, A., Osawa, R. (2018) Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure, Scientific Reports, 11(8), 435
  6. Hara, Y. K., Kobayashi, J., Yamada, R., Sasaki, D., Kuriya, Y., Hirono-Hara, Y., Ishii, J., Araki, M., Kondo, A. (2017) Transporter Engineering in Biomass Utilization by Yeast, FEMS Yeast Research, 17(7), fox061
  7. Sasaki, K., Tsuge, Y., Kawaguchi, H., Yasukawa, M., Sasaki, D., Sazuka, T., Kamio, E., Ogino, C., Matsuyama, H., Kondo, A. (2017) Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process, Applied Microbiology and Biotechnology, 101(15): 6007-6014
  8. Sasaki, K., Sasaki, D., Okai, N., Tanaka, K., Nomoto, R., Fukuda, I., Yoshida, K., Kondo, A., Osawa, R. (2017) Taurine does not affect the composition, diversity, or metabolism of human colonic microbiota simulated in a single-batch fermentation system, PLOS ONE, 12(7), e0180991
  9. Kobayashi, J., Sasaki, D., Hara, K.Y., Hasunuma, T., Kondo, A. (2017) Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae, Microbial Cell Factories, 16:44
  10. Sasaki, D., Sasaki, K.,Tsuge, Y., Kondo, A. (2016) Comparative metabolic state of microflora on the surface of the anode electrode in a microbial fuel cell operated at different pH conditions., AMB Express, 6, 125
  11. Yamamoto, K., Hara, Y. K., Morita, T., Nishimura, A., Sasaki, D., Ishii, J., Ogino, C., Kizaki, N., Kondo, A. (2016) Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes, Microbial Cell Factories,15(1):155
  12. Takagi, R., Sasaki, K., Sasaki, D., Fukuda, I., Tanaka, K., Yoshida, K., Kondo, A., Osawa, R. (2016) A Single-batch fermentation system to simulate human colonic microbiota for high-throughput evaluation of prebiotics., PLOS ONE,11, e0160533
  13. Sasaki, K., Okamoto, M., Shirai, T., Tsuge, Y., Fujino, A., Sasaki, D., Morita, M., Matsuda, F., Kikuchi, J., Kondo, A. (2016) Toward the complete utilization of rice straw: Methane fermentation and lignin recovery by a combinational process involving mechanical milling, supporting material and nanofiltration ., Bioresource Technology,216, 830-837
  14. Song, J.*, Sasaki, D.*, Sasaki, K., Kato, S., Kondo, A., Hashimoto, K., Nakanishi, N. (*Equally Contribution) (2016) Comprehensive metabolomic analyses of anode-respiring Geobacter sulfurreducens cells: the impact of anode-respiration activity on intracellular metabolite levels., Process Biochemistry, 51(1), 34-38
  15. Sasaki, K., Okamoto, M., Shirai, T., Tsuge, Y., Teramura, H., Sasaki, D. Kawaguchi, H., Hasunuma, T., Ogino, C., Matsuda, F., Kikuchi, J., Kondo, A. (2015) Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content., Biotechnol. Biofuels., 8: 88
  16. Sasaki, K., Tsuge, Y., Sasaki, D., Kawaguchi, H., Sazuka, T., Ogino, C., Kondo, A. (2015) Repeated ethanol production from sweet sorghum juice concentrated by membrane separation, Bioresource Technology, 186: 351–355
  17. Hori, T., Haruta, S., Sasaki, D., Hanajima, D., Ueno, Y., Ogata, A., Ishii, M., Igarashi, Y. (2015) Reorganization of the bacterial and archaeal populations associated with organic loading conditions in a thermophilic anaerobic digester., J . Biosci. Bioeng., 119:337–344
  18. Sasaki, D., Sasaki, K., Tsuge, Y., Morita, M., Kondo, A. (2014) Comparison of metabolomic profiles of microbial communities between stable and deteriorated methanogenic processes., Bioresour. Technol., 72: 83–90
  19. Sasaki, K., Tsuge, Y., Sasaki, D., Hasunuma, T., Sakamoto, T., Sakihama, Y., Ogino, C., Kondo, A. (2014) Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae., Bioresour. Technol., 169: 380–386
  20. Sasaki, K., Tsuge, Y., Sasaki, D., Kondo, A. (2014) Increase in lactate yield by growing Corynebacterium glutamicum in a bioelectrochemical reactor., J. Biosci. Bioeng., 117: 598–601
  21. Sasaki K, Tsuge Y, Sasaki, D., Teramura, H., Inokuma, K., Hasunuma, T., Ogino, C., Kondo, A. (2015) Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae., Bioresour. Technol., 185: 263–268
  22. Sasaki, K., Tsuge, Y., Sasaki, D., Teramura, H., Wakai, S., Kawaguchi, H., Sazuka, T., Ogino, C., Kondo, A. (2014) Increased ethanol production from sweet sorghum juice concentrated by a membrane separation process., Bioresour. Technol., 169: 821–825
  23. Sasaki, K., Sasaki, D., Sakihama, Y., Teramura, H., Yamada, R., Hasunuma, T., Ogino, C., Kondo,A. (2013) Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration., Bioresour. Technol. 147: 84–8
  24. Sasaki, D., Morita, M., Sasaki, K., Watanabe, A., Ohmura, N. (2013) Increased growth of a hydrogenotrophic methanogen in co-culture with a cellulolytic bacterium under cathodic electrochemical regulation., Biosci. Biotechnol. Biochem. 77:1096–1099
  25. Sasaki, D., Sasaki, K., Watanabe, A., Morita, M., Igarashi, Y., Ohmura, N. (2013) Efficient production of methane from artificial garbage waste by a cylindrical bioelectrochemical reactor containing carbon fiber textiles. AMB Express 3:17.
  26. Sasaki, D., Sasaki, K., Watanabe, A., Morita, M., Matsumoto, N., Igarashi, Y., Ohmura, N. (2013) Operation of a cylindrical bioelectrochemical reactor containing carbon fiber fabric for efficient methane fermentation from thickened sewage sludge., Bioresour. Technol. 129: 366–373
  27. Sasaki, K., Morita, M., Sasaki, D., Ohmura, N., Igarashi, Y. (2013) The membraneless bioelectrochemical reactor stimulates hydrogen fermentation by inhibiting methanogenic archaea., Appl. Microbiol. Biotechnol. 97: 7005–7013
  28. Sasaki, D., Morita, M., Sasaki, K., Watanabe, A., Ohmura, N. (2012) Acceleration of cellulose degradation and shift of product via methanogenic co-culture of a cellulolytic bacterium with a hydrogenotrophic methanogen., J. Biosci. Bioeng. 114:435–9
  29. Sasaki, D., Sasaki, K., Morita, M., Hirano, S., Matsumoto, N., Ohmura, N. (2012) Bioelectrochemical regulation accelerates facultatively syntrophic proteolysis., J. Biosci. Bioeng. 114: 59–63
  30. Sasaki, K., Morita, M., Matsumoto, N., Sasaki, D., Hirano, S., Ohmura, N., Igarashi, Y. (2012) Construction of hydrogen fermentation from garbage slurry using the membrane free bioelectrochemical system., J. Biosci. Bioeng. 114: 64–9
  31. Sasaki K, Morita M, Sasaki, D. Matsumoto N, Ohmura N, Igarashi Y. 2012. Single-chamber bioelectrochemical hydrogen fermentation from garbage slurry. Biochem Eng J 68:104–108.
  32. Hori T, Sasaki, D. Haruta S, Shigematsu T, Ueno Y, Ishii M, Igarashi Y. 2011. Detection of active, potentially acetate-oxidizing syntrophs in an anaerobic digester by flux measurement and formyltetrahydrofolate synthetase (FTHFS) expression profiling. Microbiology 157:1980–1989.
  33. Sasaki, D. Hori T, Haruta S, Ueno Y, Ishii M, Igarashi Y. 2011. Methanogenic pathway and community structure in a thermophilic anaerobic digestion process of organic solid waste. J Biosci Bioeng 111:41–46.
  34. Sasaki K, Hirano S-I, Morita M, Sasaki, D. Matsumoto N, Ohmura N, Igarashi Y. 2011. Bioelectrochemical system accelerates microbial growth and degradation of filter paper. Appl Microbiol Biotechnol 89:449–55.
  35. Sasaki K, Morita M, Sasaki, D. Hirano S-I, Matsumoto N, Ohmura N, Igarashi Y. 2011. Methanogenic communities on the electrodes of bioelectrochemical reactors without membranes. J Biosci Bioeng 111:47–9.
  36. Sasaki K, Morita M, Sasaki, D. Hirano S, Matsumoto N, Watanabe A, Ohmura N, Igarashi Y. 2011. A bioelectrochemical reactor containing carbon fiber textiles enables efficient methane fermentation from garbage slurry. Bioresour Technol 102:6837–42.
  37. Sasaki K, Morita M, Sasaki, D. Nagaoka J, Matsumoto N, Ohmura N, Shinozaki H. 2011. Syntrophic degradation of proteinaceous materials by the thermophilic strains Coprothermobacter proteolyticus and Methanothermobacter thermautotrophicus. J Biosci Bioeng 112:469–72.
  38. Sasaki K, Morita M, Hirano S-I, Sasaki, D. Ohmura N, Igarashi Y. 2010. Efficient degradation of rice straw in the reactors packed by carbon fiber textiles. Appl Microbiol Biotechnol 87:1579–86.
  39. Sasaki K, Sasaki, D. Morita M, Hirano S-I, Matsumoto N, Ohmura N, Igarashi Y. 2010. Bioelectrochemical system stabilizes methane fermentation from garbage slurry. Bioresour Technol 101:3415–22.
  40. Sasaki K, Sasaki, D. Morita M, Hirano S-I, Matsumoto N, Ohmura N, Igarashi Y. 2010. Efficient treatment of garbage slurry in methanogenic bioreactor packed by fibrous sponge with high porosity. Appl Microbiol Biotechnol 86:1573–83.
  41. Ueno Y, Sasaki, D. Fukui H, Haruta S, Ishii M, Igarashi Y. 2006. Changes in bacterial community during fermentative hydrogen and acid production from organic waste by thermophilic anaerobic microflora. J Appl Microbiol 101:331–343.
  42. Okuda Y, Sasaki, D. Nogami S, Kaneko Y, Ohya Y, Anraku Y. 2003. Occurrence, horizontal transfer and degeneration of VDE intein family in Saccharomycete yeasts. Yeast 20:563–73.

総説・解説等

  1. 生田直子, 篠原涼平, 佐々木大介, 佐々木建吾, 培養系ヒト腸内細菌叢モデルKUHIMMを利用した食物繊維の影響評価, New Food Industry, Vol.60, No.5, 37-43 (2018)
  2. 佐々木建吾,佐々木大介,森田仁彦,五十嵐泰夫,近藤昭彦,特集 電気化学システムを用いた模擬生ごみからの水素発酵の制御,一般社団法人 水素エネルギー協会(HESS)会誌,
    38(1),33-37 (2013)