メンバー紹介

TOP メンバー（イノベ） 佐々木　大介

特命助教佐々木　大介 Daisuke Sasaki

経歴

2008/03
東京大学大学院　農学生命科学研究科　応用生命工学専攻　博士課程修了 博士（農学）
2008/04
東京大学大学院　農学生命科学研究科　応用生命工学専攻 特任研究員
2010/04
一般財団法人電力中央研究所　バイオテクノロジー領域　特別契約研究員
2012/12
神戸大学　大学院工学研究科　応用化学専攻　特命助教
2015/04
神戸大学　自然科学系先端融合研究環　特命助教
2016/04
神戸大学大学院　科学技術イノベーション研究科　特命助教
2017/10
高機能遺伝子デザイン技術研究組合　研究員
2018/04
神戸大学大学院　科学技術イノベーション研究科　特命助教
2018/07
神戸大学　先端バイオ工学研究センター　兼任

発表論文

  1. Inokuma,K., Sasaki,D., Kurata,K., Ichikawa,M., Otsuka,Y., Kondo,A.(2023/7) Sulfated and non-sulfated chondroitin affect the composition and metabolism of human colonic microbiota simulated in an in vitro fermentation system,13:12313
  2. Kobayashi, J., Sasaki, D., Hara, KY., Hasunuma, T., Kondo, A. (2022/08) Metabolic engineering of the L-serine biosynthetic pathway improves glutathione production in Saccharomyces cerevisiae, Microbial Cell Factories, 21, 153
  3. Yoshida, N., Yamashita, T., Osone, T., Hosooka, T., Shinohara, M., Kitahama, S., Sasaki, K., Sasaki, D., Yoneshiro, T., Suzuki, T., Emoto, T., Saito, Y., Ozawa, G., Hirota, Y., Kitaura, Y., Shimomura, Y., Okamatsu-Ogura, Y., Saito, M., Kondo, A., Kajimura, S., Inagaki, T., Ogawa, W., Yamada, T., Hirata, K. (2021/11) Bacteroides spp. promotes branched-chain amino acid catabolism in brown fat and inhibits obesity, iScience, 24(11): 103342
  4. Sasaki, K., Mori, T., Hoshi, N., Sasaki, D., Inoue, J., Shinkura, R., Kondo, A. (2021/07) W27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota, Scientific Reports, 11(1): 14627
  5. Hoshi, N., Inoue, J., Sasaki, D., Sasaki, K. (2021/04) The Kobe University Human Intestinal Microbiota Model for gut intervention studies (mini review), Applied Microbiology and Biotechnology, 105(7), 2625-2632
  6. Sasaki, K., Sasaki, D., Sasaki, K., Nishidono, Y, Yamamori, A., Tanaka, K., Kondo, A. (2021/02) Growth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota, Scientific Reports, 11: 4580
  7. Sasaki, K., Sasaki, D., Tsuge, Y., Morita, M., Kondo, A. (2021/01) Enhanced methane production from cellulose using a two-stage process involving a bioelectrochemical system and a fixed film reactor, Biotechnology for Biofuels, 14: 7
  8. Nakashima, A., Sasaki, K., Sasaki, D., Yasuda, K., Suzuki, K., Kondo, A. (2021/01) The alga Euglena gracilis stimulates Faecalibacterium in the gut and contributes to increased defecation, Scientific Reports, 11, 1074
  9. Oba, S., Sunagawa, T., Tanihiro, R., Awashima, K., Sugiyama, H., Odani, T., Nakamura, Y., Kondo, A., Sasaki, D., Sasaki, K. (2020/10) Prebiotic effects of yeast mannan, which selectively promotes Bacteroides thetaiotaomicron and Bacteroides ovatus in a human colonic microbiota model, Scientific Reports, 10(1), 17351
  10. Sasaki, D., Sasaki, K., Kondo, A. (2020/10) Glycosidic linkage structures influence dietary fiber fermentability and propionate production by human colonic microbiota in vitro, Biotechnology Journal, 15(10), 1900523 (10月号表紙採用)
  11. Yoshida, N., Yamashita, T., Kishino, S., Watanabe, H., Sasaki, K., Sasaki, D., Tabata, T., Sugiyama, Y., Kitamura, N., Saito, Y., Emoto, T., Hayashi, T., Takahashi, T., Shinohara, M., Osawa, R., Kondo, A., Yamada, T., Ogawa, J., Hirata, K. (2020/08) A possible beneficial effect of Bacteroides on faecal lipopolysaccharide activity and cardiovascular diseases, Scientific Reports, 10(1), 13009
  12. Murai, K., Sasaki, D., Kobayashi, S., Yamaguchi, A., Uchikura, H., Shirai, T., Sasaki, K., Kondo, A., Tsuge, Y. (2020/07) Optimal ratio of carbon flux between glycolysis and the pentose phosphate pathway for amino acid accumulation in Corynebacterium glutamicum, ACS Synthetic Biology, 9(7), 1615-1622
  13. Sasaki, K., Sasaki, D., Hannya, A., Tsubota, J., Kondo, A. (2020/05) In vitro human colonic microbiota utilises D-β-hydroxybutyrate to increase butyrogenesis, Scientific Reports, 10, 8516
  14. Hatanaka, M., Morita, H., Aoyagi, Y., Sasaki, K., Sasaki, D., Kondo, A., Nakamura, T. (2020/05) Effective bifidogenic growth factors cyclo-Val-Leu and cyclo-Val-Ile produced by Bacillus subtilis C-3102 in the human colonic microbiota model, Scientific Reports, 10, 7591
  15. Sasaki, K., Sasaki, D., Inoue, J., Hoshi, N., Maeda, T., Yamada, R., Kondo, A. (2020/05) Bacillus coagulans SANK 70528 suppresses Enterobacteriaceae in the microbiota of ulcerative colitis in vitro and enhances butyrogenesis in healthy microbiota, Applied Microbiology and Biotechnology, 104(9), 3859-3867
  16. Sasaki, D., Sasaki, K., Kadowaki, Y., Aotsuka, Y., Kondo, A. (2019/11) Bifidogenic and butyrogenic effects of young barely leaf extract in an in vitro human colonic microbiota model, AMB Express, 9: 182
  17. Shinohara, R., Sasaki, K., Inoue, J., Hoshi, N., Fukuda, I., Sasaki, D., Kondo, A., Osawa, R. (2019/10) Butyryl-CoA:acetate CoA transferase gene associated with the genus Roseburia is decreased in the gut microbiota of Japanese patients with ulcerative colitis, Bioscience of Microbiota, Food and Health, 38(4), 159-163
  18. Yoshida, N., Sasaki, K., Sasaki, D., Yamashita, T., Fukuda, H., Hayashi, T., Tabata, T., Osawa, R., Hirata, K., Kondo, A. (2019/08) Effect of resistant starch on the gut microbiota and its metabolites in patients with coronary artery disease, Journal of Atherosclerosis and Thrombosis, 26(8): 705-719
  19. Sasaki, K., Inoue, J., Sasaki, D., Hoshi, N., Shirai, T., Fukuda, I., Azuma, T., Kondo, A., Osawa, R. (2019/05) Construction of a model culture system of human colonic microbiota to detect decreased Lachnospiraceae abundance and butyrogenesis in the feces of ulcerative colitis patients, Biotechnology Journal, 14: 1800555 (5月号Inside Front Cover採用)
  20. Sasaki, D., Sasaki, K., Tsuge, Y., Kondo, A. (2019/04) Less biomass and intracellular glutamate in anodic biofilms lead to efficient electricity generation by microbial fuel cells, Biotechnology for Biofuels, 12: 72
  21. Kobayashi, J., Sasaki, D., Bamba, T., Hasunuma, T., Kondo, A. (2019/02) Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae, Applied Microbiology and Biotechnology, 103(3), 1243-1254
  22. Inokuma, K., Matsuda M., Sasaki, D., Hasunuma, T., Kondo, A. (2018/09) Widespread effect of N-acetyl-D-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis, Microbial Cell Factories, 17, 153
  23. Kobayashi, J., Sasaki, D., Kondo, A. (2018/06) A procedure for precise determination of glutathione produced by Saccharomyces cerevisiae, Bio-protocol, 8(12): e2887
  24. Sasaki, K., Sasaki, D., Tsuge, Y., Morita, M., Kondo, A. (2018/06) Changes in the microbial consortium during dark hydrogen fermentation in a bioelectrochemical system increases methane production during a two-stage process, Biotechnology for Biofuels, 11: 173
  25. Sasaki, K., Sasaki, D., Kamiya, K., Nakanishi, S., Kondo, A., Kato, S. (2018/04) Electrochemical biotechnologies minimizing the required electrode assemblies, Current Opinion in Biotechnology, 50, 182-188
  26. Sasaki, D., Sasaki, K., Ikuta, N., Yasuda, T., Fukuda, I., Kondo, A., Osawa, R. (2018/01) Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure, Scientific Reports, 8, 435
  27. Hara, Y.K., Kobayashi, J., Yamada, R., Sasaki, D., Kuriya, Y., Hirono-Hara, Y., Ishii, J., Araki, M., Kondo, A. (2017/11) Transporter Engineering in Biomass Utilization by Yeast, FEMS Yeast Research, 17(7), fox061
  28. Sasaki, K., Tsuge, Y., Kawaguchi, H., Yasukawa, M., Sasaki, D., Sazuka, T., Kamio, E., Ogino, C., Matsuyama, H., Kondo, A. (2017/08) Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process, Applied Microbiology and Biotechnology, 101(15): 6007-6014
  29. Sasaki, K., Sasaki, D., Okai, N., Tanaka, K., Nomoto, R., Fukuda, I., Yoshida, K., Kondo, A., Osawa, R. (2017/07) Taurine does not affect the composition, diversity, or metabolism of human colonic microbiota simulated in a single-batch fermentation system, PLOS ONE, 12(7), e0180991
  30. Kobayashi, J., Sasaki, D., Hara, K.Y., Hasunuma, T., Kondo, A. (2017/03) Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae, Microbial Cell Factories, 16: 44
  31. Sasaki, D., Sasaki, K.,Tsuge, Y., Kondo, A. (2016/12) Comparative metabolic state of microflora on the surface of the anode electrode in a microbial fuel cell operated at different pH conditions, AMB Express, 6, 125
  32. Yamamoto, K., Hara, Y.K., Morita, T., Nishimura, A., Sasaki, D., Ishii, J., Ogino, C., Kizaki, N., Kondo, A. (2016/09) Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes, Microbial Cell Factories, 15(1): 155
  33. Sasaki, K., Okamoto, M., Shirai, T., Tsuge, Y., Fujino, A., Sasaki, D., Morita, M., Matsuda, F., Kikuchi, J., Kondo, A. (2016/09) Toward the complete utilization of rice straw: Methane fermentation and lignin recovery by a combinational process involving mechanical milling, supporting material and nanofiltration, Bioresource Technology, 216, 830-837
  34. Takagi, R., Sasaki, K., Sasaki, D., Fukuda, I., Tanaka, K., Yoshida, K., Kondo, A., Osawa, R. (2016/08) A Single-batch fermentation system to simulate human colonic microbiota for high-throughput evaluation of prebiotics, PLOS ONE, 11, e0160533
  35. Song, J.*, Sasaki, D.*, Sasaki, K., Kato, S., Kondo, A., Hashimoto, K., Nakanishi, N. (*Equally Contribution) (2016/01) Comprehensive metabolomic analyses of anode-respiring Geobacter sulfurreducens cells: the impact of anode-respiration activity on intracellular metabolite levels, Process Biochemistry, 51(1), 34-38
  36. Sasaki, K., Okamoto, M., Shirai, T., Tsuge, Y., Teramura, H., Sasaki, D., Kawaguchi, H., Hasunuma, T., Ogino, C., Matsuda, F., Kikuchi, J., Kondo, A. (2015/06) Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content, Biotechnol. Biofuels, 8: 88
  37. Sasaki, K., Tsuge, Y., Sasaki, D., Kawaguchi, H., Sazuka, T., Ogino, C., Kondo, A. (2015/06) Repeated ethanol production from sweet sorghum juice concentrated by membrane separation, Bioresource Technology, 186: 351–355
  38. Sasaki, K., Tsuge, Y., Sasaki, D., Teramura, H., Inokuma, K., Hasunuma, T., Ogino, C., Kondo, A. (2015/06) Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae, Bioresour. Technol., 185: 263–268
  39. Hori, T., Haruta, S., Sasaki, D., Hanajima, D., Ueno, Y., Ogata, A., Ishii, M., Igarashi, Y. (2015/03) Reorganization of the bacterial and archaeal populations associated with organic loading conditions in a thermophilic anaerobic digester, J. Biosci. Bioeng., 119: 337–344
  40. Sasaki, D., Sasaki, K., Tsuge, Y., Morita, M., Kondo, A. (2014/11) Comparison of metabolomic profiles of microbial communities between stable and deteriorated methanogenic processes, Bioresour. Technol., 72: 83–90
  41. Sasaki, K., Tsuge, Y., Sasaki, D., Hasunuma, T., Sakamoto, T., Sakihama, Y., Ogino, C., Kondo, A. (2014/10) Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae, Bioresour. Technol., 169: 380–386
  42. Sasaki, K., Tsuge, Y., Sasaki, D., Teramura, H., Wakai, S., Kawaguchi, H., Sazuka, T., Ogino, C., Kondo, A. (2014/10) Increased ethanol production from sweet sorghum juice concentrated by a membrane separation process, Bioresour. Technol., 169: 821–825
  43. Sasaki, K., Tsuge, Y., Sasaki, D., Kondo, A. (2014/05) Increase in lactate yield by growing Corynebacterium glutamicum in a bioelectrochemical reactor, J. Biosci. Bioeng., 117: 598–601
  44. Sasaki, K., Sasaki, D., Sakihama, Y., Teramura, H., Yamada, R., Hasunuma, T., Ogino, C., Kondo, A. (2013/11) Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration, Bioresour. Technol., 147: 84–8
  45. Sasaki, K., Morita, M., Sasaki, D., Ohmura, N., Igarashi, Y. (2013/08) The membraneless bioelectrochemical reactor stimulates hydrogen fermentation by inhibiting methanogenic archaea, Appl. Microbiol. Biotechnol., 97: 7005–7013
  46. Sasaki, D., Sasaki, K., Watanabe, A., Morita, M., Igarashi, Y., Ohmura, N. (2013/03) Efficient production of methane from artificial garbage waste by a cylindrical bioelectrochemical reactor containing carbon fiber textiles, AMB Express, 3: 17
  47. Sasaki, D., Sasaki, K., Watanabe, A., Morita, M., Matsumoto, N., Igarashi, Y., Ohmura, N. (2013/02) Operation of a cylindrical bioelectrochemical reactor containing carbon fiber fabric for efficient methane fermentation from thickened sewage sludge, Bioresour. Technol., 129: 366–373
  48. Sasaki, D., Morita, M., Sasaki, K., Watanabe, A., Ohmura, N. (2013/01) Increased growth of a hydrogenotrophic methanogen in co-culture with a cellulolytic bacterium under cathodic electrochemical regulation, Biosci. Biotechnol. Biochem., 77: 1096–1099
  49. Sasaki, D., Morita, M., Sasaki, K., Watanabe, A., Ohmura, N. (2012/10) Acceleration of cellulose degradation and shift of product via methanogenic co-culture of a cellulolytic bacterium with a hydrogenotrophic methanogen, J. Biosci. Bioeng., 114: 435–9
  50. Sasaki, K., Morita, M., Sasaki, D., Matsumoto, N., Ohmura, N., Igarashi, Y. (2012/10) Single-chamber bioelectrochemical hydrogen fermentation from garbage slurry, Biochem. Eng. J., 68: 104–108
  51. Sasaki, D., Sasaki, K., Morita, M., Hirano, S., Matsumoto, N., Ohmura, N. (2012/07) Bioelectrochemical regulation accelerates facultatively syntrophic proteolysis, J. Biosci. Bioeng., 114: 59–63
  52. Sasaki, K., Morita, M., Matsumoto, N., Sasaki, D., Hirano, S., Ohmura, N., Igarashi, Y. (2012/07) Construction of hydrogen fermentation from garbage slurry using the membrane free bioelectrochemical system, J. Biosci. Bioeng., 114: 64–9
  53. Sasaki, K., Morita, M., Sasaki, D., Nagaoka, J., Matsumoto, N., Ohmura, N., Shinozaki, H. (2011/11) Syntrophic degradation of proteinaceous materials by the thermophilic strains Coprothermobacter proteolyticus and Methanothermobacter thermautotrophicus, J. Biosci. Bioeng., 112: 469–72
  54. Hori, T., Sasaki, D., Haruta, S., Shigematsu, T., Ueno, Y., Ishii, M., Igarashi, Y. (2011/07) Detection of active, potentially acetate-oxidizing syntrophs in an anaerobic digester by flux measurement and formyltetrahydrofolate synthetase (FTHFS) expression profiling, Microbiology, 157: 1980–1989
  55. Sasaki, K., Morita, M., Sasaki, D., Hirano, S., Matsumoto, N., Watanabe, A., Ohmura, N., Igarashi, Y. (2011/07) A bioelectrochemical reactor containing carbon fiber textiles enables efficient methane fermentation from garbage slurry, Bioresour Technol., 102: 6837–42
  56. Sasaki, D., Hori, T., Haruta, S., Ueno, Y., Ishii, M., Igarashi, Y. (2011/01) Methanogenic pathway and community structure in a thermophilic anaerobic digestion process of organic solid waste, J. Biosci. Bioeng., 111: 41–46
  57. Sasaki, K., Hirano, S-I., Morita, M., Sasaki, D., Matsumoto, N., Ohmura, N., Igarashi, Y. (2011/01) Bioelectrochemical system accelerates microbial growth and degradation of filter paper, Appl. Microbiol. Biotechnol., 89: 449–55
  58. Sasaki, K., Morita, M., Sasaki, D., Hirano, S-I., Matsumoto, N., Ohmura, N., Igarashi, Y. (2011/01) Methanogenic communities on the electrodes of bioelectrochemical reactors without membranes, J. Biosci. Bioeng., 111: 47–9
  59. Sasaki, K., Morita, M., Hirano, S-I., Sasaki, D., Ohmura, N., Igarashi, Y. (2010/07) Efficient degradation of rice straw in the reactors packed by carbon fiber textiles, Appl. Microbiol. Biotechnol., 87: 1579–86
  60. Sasaki, K., Sasaki, D., Morita, M., Hirano, S-I., Matsumoto, N., Ohmura, N., Igarashi, Y. (2010/05) Bioelectrochemical system stabilizes methane fermentation from garbage slurry, Bioresour Technol., 101: 3415–22
  61. Sasaki, K., Sasaki, D., Morita, M., Hirano, S-I., Matsumoto, N., Ohmura, N., Igarashi, Y. (2010/05) Efficient treatment of garbage slurry in methanogenic bioreactor packed by fibrous sponge with high porosity, Appl. Microbiol. Biotechnol., 86: 1573–83
  62. Ueno, Y., Sasaki, D., Fukui, H., Haruta, S., Ishii, M., Igarashi, Y. (2006/08) Changes in bacterial community during fermentative hydrogen and acid production from organic waste by thermophilic anaerobic microflora, J. Appl. Microbiol., 101: 331–343
  63. Okuda, Y., Sasaki, D., Nogami, S., Kaneko, Y., Ohya, Y., Anraku, Y. (2003/05) Occurrence, horizontal transfer and degeneration of VDE intein family in Saccharomycete yeasts, Yeast, 20: 563–73

総説・解説等

  1. 佐々木建吾, 佐々木大介, 近藤昭彦, 電気化学的前処理／通電式担体充填による2段発酵を廃水・廃棄物処理へ適用, 化学と生物, 59(12), 247-253 (2021/05)
  2. 佐々木建吾, 佐々木大介, in vitroヒト大腸細菌叢モデルによる構造の異なる食物繊維の発酵性評価法, 生化学，第93巻，第1号，167-172, (2021/02)
  3. 佐々木大介, 佐々木建吾, ヒト腸内細菌叢モデルによるプレバイオティクスの評価, アレルギーの臨床, Vol.39(9), 53(759)-58(764) (2019/08)
  4. 佐々木大介, 佐々木建吾, ヒト腸内細菌叢モデルによるプレバイオティクスの評価, アグリバイオ, Vol.3(9), 53-58 (2019/08)
  5. Kobayashi, J., Sasaki, D., Hara, K.Y., Kondo, A. Genetic engineering of microorganisms for high glutathione production, Glutathione: Biosynthesis, Functions and Biological Implications, Nova Science Publishers, 83-103 (2019/02)
  6. 佐々木大介, 佐々木建吾, 近藤昭彦, 大澤朗, in vitro培養システムによる食物繊維のヒト腸管細菌叢への影響評価, ルミナコイド研究（日本食物繊維学会会誌）, Vol22, No.2, 63-74 (2018/12)
  7. 生田直子, 篠原涼平, 佐々木大介, 佐々木建吾, 培養系ヒト腸内細菌叢モデルKUHIMMを利用した食物繊維の影響評価, New Food Industry, Vol.60, No.5, 37-43 (2018/05)
  8. 佐々木建吾，佐々木大介，森田仁彦，五十嵐泰夫，近藤昭彦，特集 電気化学システムを用いた模擬生ごみからの水素発酵の制御，一般社団法人 水素エネルギー協会(HESS)会誌，38(1)，33-37 (2013/03)