メンバー紹介

教授西田 敬二 Keiji Nishida

経歴

2001/03
東京大学 理学部生物科学科 卒業
2003/03
東京大学大学院 理学系研究科 修士課程生物科学専攻修了
2003/04
日本学術振興会特別研究員(DC1)
2006/03
東京大学大学院 理学系研究科 博士課程生物科学専攻修了
2006/04
立教大学 理学部 博士研究員
2006/04
日本学術振興会特別研究員(PD)
2008/04
ハーバード大学 医学部 博士研究員
2009/04
日本学術振興会海外特別研究員
2013/06
神戸大学 自然科学系先端融合研究環重点研究部 特命准教授
2016/04
神戸大学大学院 科学技術イノベーション研究科 特命准教授
2016/11
神戸大学大学院 科学技術イノベーション研究科 教授
2018/07
神戸大学 先端バイオ工学研究センター 主配置
神戸大学大学院 科学技術イノベーション研究科 兼任

受賞等

  • 2017 科学技術への顕著な貢献 2017(ナイスステップな研究者) に選定
  • 2017 第9回学長表彰(財務貢献者) 受賞
  • 2016 International Botanical Congress Excellent Scholar Award 受賞
  • 2008 日本植物学会若手奨励賞 受賞
  • 2006 日本植物形態学会奨励賞 受賞

発表論文

  1. Nambu-Nishida, Y., Nishida, K., Hasunuma, T., Kondo, A. (2018) Genetic and physiological basis for antibody production by Kluyveromyces marxianus, AMB Express, 8: 56
  2. Banno, S., Nishida, K., Arazoe, T., Mitsunobu, H., Kondo, A. (2018) Deaminase-mediated multiplex genome editing in Escherichia coli, Nature Microbiology, 3, 423-429
  3. Nambu-Nishida, Y., Nishida, K., Hasunuma, T., Kondo, A. (2017) Development of a comprehensive set of tools for genome engineering in a cold- and thermo-tolerant Kluyveromyces marxianus yeast strain, Scientific Reports, 7(1): 8993
  4. Mitsunobu, H., Teramoto, J., Nishida, K., Kondo, A. (Review)(2017) Beyond native Cas9: manipulating genomic information and function, Trends in Biotechnology, 35(10), 983-996
  5. Arazoe, T., Nishida, K., Kondo, A. (2017) Targeted nucleotide substitution in mammalian cell by Target-AID, Bio-protocol, 7: 11
  6. Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., Ezura, H., Nishida, K., Ariizumi, T., Kondo, A. (2017) Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion, Nature Biotechnology, 35(5), 441-443
  7. Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki, M., Hara, Y. K., Shimatani, Z., Kondo, A. (2016) Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems., Science,353, aaf8729
  8. Hara, K.Y., Aoki, N., Kobayashi, J., Kiriyama, K., Nishida K., Araki , M., Kondo, A. (2015) Improvement of oxidized glutathione fermentation by thiol redox metabolism engineering in Saccharomyces cerevisiae., Applied Microbiology and Biotechnology, 99(22), 9771-9778
  9. Ye, X., Morikawa, K., Ho, S.H., Nishida K., Araki, M., Hasunuma, T., Hara, K.Y., Kondo, A. (2015)   Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity., Microbial Cell Factories, 14: 56
  10. Imoto Y, Kuroiwa H, Yoshida Y, Ohnuma M, Fujiwara T, Yoshida M, Nishida K, Yagisawa F, Hirooka S, Miyagishima SY, Misumi O, Kawano S, Kuroiwa T. Single-membrane-bounded peroxisome division revealed by isolation of dynamin-based machinery. Proc Natl Acad Sci U S A. 2013 Jun 4;110(23):9583-8. doi: 10.1073/pnas.1303483110.
  11. Yagisawa F, Fujiwara T, Ohnuma M, Kuroiwa H, Nishida K, Imoto Y, Yoshida Y, Kuroiwa T. Golgi inheritance in the primitive red alga, Cyanidioschyzon merolae. Protoplasma. [Epub ahead of print] (2012)
  12. Nishida K, Silver PA Induction of Biogenic Magnetization and Redox Control by a Component of the Target of Rapamycin Complex 1 Signaling Pathway. Plos Biology. 10(2): e1001269. (2012) Editors pick of the journal, highlighted in Nature Methods, and cited by several public news articles as "Magnetic yeast".
  13. Yagisawa F, Fujiwara T, Kuroiwa H, Nishida K, Imoto Y, Kuroiwa T. Mitotic inheritance of endoplasmic reticulum in the primitive red alga Cyanidioschyzon merolae. Protoplasma. Oct.249(4): 1129-35 (2012)
  14. Imoto Y, Nishida K, Yagisawa F, Yoshida Y, Ohnuma M, Yoshida M, Fujiwara T, Kuroiwa H, Kawano S, Kuroiwa T. Involvement of Elongation Factor-1α in Cytokinesis without Actomyosin Contractile Ring in the Primitive Red Alga Cyanidioschyzon merolae. Cytologia. Vol. 76 No. 4: 431-437 (2011)
  15. Fujiwara T, Kuroiwa H, Yagisawa F, Ohnuma M, Yoshida Y, Yoshida M, Nishida K, Misumi O, Watanabe S, Tanaka K, Kuroiwa T. The coiled-coil protein VIG1 is essential for tethering vacuoles to mitochondria during vacuole inheritance of Cyanidioschyzon merolae. Plant Cell. Mar;22 (3):772-81.(2010)
  16. Fujiwara T, Yagisawa F, Ohnuma M, Yoshida Y, Yoshida M, Nishida K, Misumi O, Kuroiwa H, Kuroiwa T. The Vacuole Binding to Mitochondria by VIG1 Contributes an Equal Inheritance of the Vacuoles in Cyanidioschyzon merolae. Cytologia., 69(1): 97-100. (2010)
  17. Hirooka S, Misumi O, Yoshida M, Mori T, Nishida K, Yagisawa F, Yoshida Y, Fujiwara T, Kuroiwa H, Kuroiwa T.Expression of the Cyanidioschyzon merolae stromal ascorbate peroxidase in Arabidopsis thaliana enhances thermotolerance. Plant Cell Rep. Dec;28(12):1881-93.(2009)
  18. Yagisawa F, Nishida K, Yoshida M, Ohnuma M, Shimada T, Fujiwara T, Yoshida Y, Misumi O, Kuroiwa H, Kuroiwa T. Identification of novel proteins in isolated polyphosphate vacuoles in the primitive red alga Cyanidioschyzon merolae. Plant J. Dec;60(5):882-93. (2009)
  19. Fujiwara T, Misumi O, Tashiro K, Yoshida Y, Nishida K, Yagisawa F, Imamura S, Yoshida M, Mori T, Tanaka K, Kuroiwa H, Kuroiwa T. Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga Cyanidioschyzon merolae. DNA Res. Feb;16(1):59-72. (2009)
  20. Yoshida Y, Nishida K, Kuroiwa T. Kawano S. Novel Dynamics of FtsZ Ring Before Plastid Abscission. Cytologia. 73(1): 197-201. (2008)
  21. Misumi O, Yoshida Y, Nishida K, Fujiwara T, Sakajiri T, Hirooka S, Nishimura Y, Kuroiwa T. Genome analysis and its significance in four unicellular algae, Cyanidioschyzon merolae, Ostreococcus tauri, Chlamydomonas reinhardtii, and Thalassiosira pseudonana. J Plant Res. Jan;121(1):3-17.(2008)
  22. Nozaki H, Takano H, Misumi O, Terasawa K, Matsuzaki M, Maruyama S, Nishida K, Yagisawa F, Yoshida Y, Fujiwara T, Takio S, Tamura K, Chung SJ, Nakamura S, Kuroiwa H, Tanaka K, Sato N, Kuroiwa T. A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae. BMC Biol. Jul 10;5:28.( 2007)
  23. Nishida K, Yagisawa F, Kuroiwa H, Yoshida Y, Kuroiwa T. WD40 protein Mda1 is purified with Dnm1 and forms a dividing ring for mitochondria before Dnm1 in Cyanidioschyzon merolae. Proc Natl Acad Sci U S A. 104(11):4736-41. (2007)
  24. Yagisawa F, Nishida K, Kuroiwa H, Nagata T, Kuroiwa T. Identification and mitotic partitioning strategies of vacuoles in the unicellular red alga Cyanidioschyzon merolae. Planta. 226(4):1017-29. (2007)
  25. Yoshida Y, Kuroiwa H, Misumi O, Nishida K, Yagisawa F, Fujiwara T, Nanamiya H, Kawamura F, Kuroiwa T. Isolated chloroplast division machinery can actively constrict after stretching. Science. Sep 8; 313(5792):1435-8.(2006)
  26. Yagisawa F, Nishida K, Kuroiwa H, Nagata T, Kuroiwa T. Identification of Lysosome-like Structures in a Unicellular Red Alga Cyanidioschyzon merolae. Cytologia. 70(1): 351-354 (2005) 
  27. Nishida K, Yagisawa F, Kuroiwa H, Nagata T, Kuroiwa T. Cell Cycle-regulated, Microtubule-independent Organelle Division in Cyanidioschyzon merolae. Mol Biol Cell. 16(5):2493-502. (2005)
  28. Misumi O, Matsuzaki M, Nozaki H, Miyagishima SY, Mori T, Nishida K, Yagisawa F, Yoshida Y, Kuroiwa H, Kuroiwa T. Cyanidioschyzon merolae genome. A tool for facilitating comparable studies on organelle biogenesis in photosynthetic eukaryotes. Plant Physiol. Feb;137(2):567-85.(2005)
  29. Yagisawa F, Nishida K, Okano Y, Minoda A, Tanaka K, Kuriowa T. Isolation of Cycloheximide-resistant mutants of Cyanidioschyzon merolae. Cytologia. 69(1): 97-100. (2004)
  30. Nishida K, Misumi O, Yagisawa F, Kuroiwa H, Nagata T, Kuroiwa T. Triple immunofluorescent labeling of FtsZ, dynamin, and EF-Tu reveals a loose association between the inner and outer membrane mitochondrial division machinery in the red alga Cyanidioschyzon merolae. J Histochem Cytochem. 52(7):843-9. (2004)
  31. Matsuzaki M, Misumi O, Shin-I T, Maruyama S, Takahara M, Miyagishima SY, Mori T, Nishida K, Yagisawa F, Nishida K, Yoshida Y, Nishimura Y, Nakao S, Kobayashi T, Momoyama Y, Higashiyama T, Minoda A, Sano M, Nomoto H, Oishi K, Hayashi H, Ohta F, Nishizaka S, Haga S, Miura S, Morishita T, Kabeya Y, Terasawa K, Suzuki Y, Ishii Y, Asakawa S, Takano H, Ohta N, Kuroiwa H, Tanaka K, Shimizu N, Sugano S, Sato N, Nozaki H, Ogasawara N, Kohara Y, Kuroiwa T. Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature. Apr 8; 428(6983): 653-7.(2004)
  32. Miyagishima SY, Nozaki H, Nishida K, Nishida K, Matsuzaki M, Kuroiwa T. Two types of FtsZ proteins in mitochondria and red-lineage chloroplasts: the duplication of FtsZ is implicated in endosymbiosis. J Mol Evol. 2004 Mar;58(3):291-303.(2004)
  33. Miyagishima SY, Nishida K, Mori T, Matsuzaki M, Higashiyama T, Kuroiwa H, Kuroiwa T. A plant-specific dynamin-related protein forms a ring at the chloroplast division site. Plant Cell. 15(3):655-65. (2003)
  34. Nishida K, Takahara M, Miyagishima SY, Kuroiwa H, Matsuzaki M, Kuroiwa T. Dynamic recruitment of dynamin for final mitochondrial severance in a primitive red alga. Proc Natl Acad Sci U S A. 100(4):2146-51. (2003)

総説・解説等

  1. 西田敬二,進展するゲノム編集, 現代化学, 1月号, No.562, <特集>生命をつくる:ゲノム編集の時代, 26-29 (2018)
  2. 西田敬二,第1章 3) 切断を伴わないゲノム編集ツール開発(デアミナーゼ他),『All About ゲノム編集』実験医学増刊号, 34(20), 羊土社,35-41(2016)
  3. 近藤昭彦,西田敬二,荒添貴之,第3章 微生物でのゲノム編集の利用と拡大技術,ゲノム編集入門,裳華房,40-55(2016)
  4. Kuroiwa T, Nishida K, Yoshida Y, Fujiwara T, Mori T, Kuroiwa H, Misumi O. Structure, function and evolution of the mitochondrial division apparatus. Biochim Biophys Acta., 1763(5-6):510-21. (2006)
  5. Nishida K, Nagata T, Kuroiwa T. Cell cycle regulated organelles division in a primitive red alga. Plant Morphology, 17(1);51-55. (2005)
  6. Miyagishima SY, Nishida K, Kuroiwa T. An evolutionary puzzle: chloroplast and mitochondrial division rings. Trends Plant Sci. 8 (9):432-8. (2003)