メンバー紹介

TOP メンバー（先端バイオ） 那須野　亮

特命准教授那須野　亮 Ryo Nasuno

経歴

2002/03
同志社大学　文学部　文化学科　心理学専攻　卒業
2006/03
昭和大学　薬学部　薬学科　卒業
2006/04
住友精化株式会社　機能樹脂研究所　研究員
2007/07
株式会社アルカ　薬剤師
2010/03
奈良先端科学技術大学院大学　バイオサイエンス研究科　細胞生物学専攻　博士前期課程　修了
2013/03
奈良先端科学技術大学院大学　バイオサイエンス研究科　細胞生物学専攻　博士後期課程　研究指導認定退学
2013/06
博士（バイオサイエンス）（奈良先端科学技術大学院大学）
2013/04
奈良先端科学技術大学院大学　バイオサイエンス研究科　研究員
2013/07
奈良先端科学技術大学院大学　バイオサイエンス研究科　博士研究員
2016/05
奈良先端科学技術大学院大学　バイオサイエンス研究科　助教
2017/04
Cleveland Clinic, Lerner Research Institute, Postdoctoral fellow（兼任）
2018/04
奈良先端科学技術大学院大学　先端科学技術研究科　バイオサイエンス領域　助教
2023/04
神戸大学　先端バイオ工学研究センター　特命准教授

受賞等

  • 2018年　第18回 日本NO学会 Young Investigator Award優秀賞（日本NO学会）
  • 2017年　平成29年度JB論文賞（日本生化学会）
  • 2012年　第85回 日本生化学会大会 鈴木紘一メモリアル賞（日本生化学会）

発表論文

  1. Hidese, R., Sakai, K., Takenaka, M., Fushimi, K., Kudo, H., Tanaka, K., Nasuno, R., Vavricka, CJ.,Kondo, A., Hasunuma, T. (2025/06) Identification of subfamily specific residues within highly active and promiscuous alcohol dehydrogenases, ACS Catalysis, 15, 11931-11943
  2. S. Shino*, R. Nasuno*,#, H. Takagi#, (*同等筆頭著者, #同等責任著者) (2022) S-glutathionylation of fructose-1,6-bisphosphate aldolase confers nitrosative stress tolerance on yeast cells via a metabolic switch, Free Radical Biology & Medicine, 193, 319-329
  3. S. Oiki, R. Nasuno, S. Urayama, H. Takagi, D. Hagiwara, (2022) Intracellular production of reactive oxygen species and a DAF-FM-related compound in Aspergillus fumigatus in response to antifungal agent exposure, Scientific Reports, 12(1), 13516
  4. R. Nasuno#, Y. Yoshikawa, H. Takagi, (#責任著者) (2022) Acetaldehyde reacts with a fluorescent nitric oxide probe harboring an o-phenylenediamine structure that interferes with fluorometry, Free Radical Biology & Medicine, 187, 29-37
  5. R. Nasuno, N. Iwai, H. Takagi, (2022) Development of a microtiter plate-based analysis method of nitric oxide dioxygenase activity, The Journal of General and Applied Microbiology, 68(1), 38-41 (2022)
  6. S. Eknikom*, R. Nasuno*,#, H. Takagi,# (*同等筆頭著者, #同等責任著者) (2022) Molecular mechanism of ethanol fermentation inhibition via protein tyrosine nitration of pyruvate decarboxylase by reactive nitrogen species in yeast, Scientific Reports, 12(1), 4664
  7. R. Nasuno, S. Suzuki, S. Oiki, D. Hagiwara, H, Takagi, (2022) Identification and Functional Analysis of GTP Cyclohydrolase II in Candida glabrata in Response to Nitrosative Stress, Frontiers in Microbiology, 13, 825121
  8. R. Nasuno, N. Yoshioka, Y. Yoshikawa, H. Takagi, (2021) Cysteine residues in the fourth zinc finger are important for activation of the nitric oxide-inducible transcription factor Fzf1 in the yeast Saccharomyces cerevisiae, Genes to Cells, 26(10), 823-829
  9. Y. Yoshikawa*, R. Nasuno*, H. Takagi, (*同等筆頭著者) (2021) NADPH is important for isobutanol tolerance in a minimal medium of Saccharomyces cerevisiae, Bioscience, Biotechnology, and Biochemistry, 85(9), 2084-2088
  10. Y. Yoshikawa*, R. Nasuno*, H. Takagi, (*同等筆頭著者) (2021) An NADPH-independent mechanism enhances oxidative and nitrosative stress tolerance in yeast cells lacking glucose-6-phosphate dehydrogenase activity, Yeast, 38(7), 414-423
  11. R. Nasuno, Y. Yoshikawa, H. Takagi, (2021) The analytical method to identify the nitrogen source for nitric oxide synthesis, Bioscience, Biotechnology, and Biochemistry, 85(2), 211-214
  12. M. Ohashi, R. Nasuno, S. Isogai, H. Takagi, (2020) High-level production of ornithine by expression of the feedback inhibition-insensitive N-acetyl glutamate kinase in the sake yeast Saccharomyces cerevisiae, Metabolic engineering, 62, 1-9
  13. R. Nasuno, S. Shino, Y. Yoshikawa, N. Yoshioka, Y. Sato, K. Kamiya, H. Takagi, (2020) Detection system of the intracellular nitric oxide in yeast by HPLC with a fluorescence detector, Analytical Biochemistry, 598, 113707
  14. K. Anam*, R. Nasuno*, H. Takagi, (*同等筆頭著者) (2020) A novel mechanism for nitrosative stress tolerance dependent on GTP cyclohydrolase II activity involved in riboflavin synthesis of yeast, Scientific Reports, 10(1), 6015
  15. A. Nishimura, R. Nasuno, Y. Yoshikawa, M. Jung, T. Ida, T. Matsunaga, M. Morita, H. Takag, H. Motohashi, T. Akaike, (2019) Mitochondrial cysteinyl-tRNA synthetase is expressed via alternative transcriptional initiation regulated by energy metabolism in yeast cells, The Journal of Biological Chemistry, 294(37), 13781-13788
  16. M. Ohashi, R. Nasuno, D. Watanabe, H. Takagi, (2019) Stable N-acetyltransferase Mpr1 improves ethanol productivity in the sake yeast Saccharomyces cerevisiae, Journal of Industrial Microbiology & Biotechnology, 46(7), 1039-1045
  17. T. Abe, Y. Toyokawa, Y. Sugimoto, H. Azuma, K. Tsukahara, R. Nasuno, D. Watanabe, M. Tsukahara, H. Takagi, (2019) Characterization of a New Saccharomyces cerevisiae Isolated from Hibiscus Flower and Its Mutant With L-Leucine Accumulation for Awamori Brewing, Frontiers in Genetics, 10, 490
  18. R.I. Astuti*, R. Nasuno*, H. Takagi, (*同等筆頭著者) (2016) Nitric oxide signaling in yeast, Applied Microbiology and Biotechnology, 100(22), 9483-9497
  19. Y. Yoshikawa*, R. Nasuno*, N. Kawahara, A. Nishimura, D. Watanabe, H. Takagi, (*同等筆頭著者) (2016) Regulatory mechanism of the flavoprotein Tah18-dependent nitric oxide synthesis and cell death in yeast, Nitric Oxide-Biology and Chemistry, 57, 85-91
  20. R. Nasuno, S. Hirase, S. Norifune, D. Watanabe, H. Takagi, (2016) Structure-based molecular design for thermostabilization of N-acetyltransferase Mpr1 involved in a novel pathway of L-arginine synthesis in yeast, Journal of Biochemistry, 159(2), 271-277
  21. H. Takagi, K. Hashida, D. Watanabe, R. Nasuno, M. Ohashi, T. Iha, M. Nezuo, M. Tsukahara, (2015) Isolation and characterization of awamori yeast mutants with L-leucine accumulation that overproduce isoamyl alcohol, Journal of Bioscience and Bioengineering, 119(2), 140-147
  22. R. Nasuno, M. Aitoku, Y. Manago, A. Nishimura, Y. Sasano, H. Takagi, (2014) Nitric oxide-mediated antioxidative mechanism in yeast through the activation of the transcription factor Mac1, PLoS One, 9(11), e113788
  23. D. Watanabe, R. Kikushima, M. Aitoku, A. Nishimura, I. Ohtsu, R. Nasuno, H. Takagi, (2014) Exogenous addition of histidine reduces copper availability in the yeast Saccharomyces cerevisiae, Microbial Cell, 1(7), 241-246
  24. R. Nasuno, Y. Hirano, T. Itoh, T. Hakoshima, T. Hibi, H. Takagi, (2013) Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism, Proceedings of the National Academy of Sciences of the United States of America, 110(29), 11821-11826
  25. B.T.M. Hoa, T. Hibi, R. Nasuno, G. Matsuo, Y. Sasano, H. Takagi, (2012) Production of N-acetyl cis-4-hydroxy-L-proline by the yeast N-acetyltransferase Mpr1, Journal of Bioscience and Bioengineering, 114(2), 160-165
  26. A. Nishimura, R. Nasuno, H. Takagi, (2012) The proline metabolism intermediate Δ1-pyrroline-5-carboxylate directly inhibits the mitochondrial respiration in budding yeast, FEBS Letters, 586(16), 2411-2416

総説・解説等

  1. 那須野亮, 高木博史, 酵母に見出したN-アセチルトランスフェラーゼMpr1の分子機能, 酵素工学ニュース, 82,32-38 (2019)
  2. 那須野亮, 吉川雄樹, 高木博史, 酵母における一酸化窒素シグナルを介したストレス応答機構, 生化学, 90(5), 701-705 (2018)
  3. R.I. Astuti*, R. Nasuno*, H. Takagi, (*同等筆頭著者) Nitric Oxide and Other Small Signalling Molecules (Chapter 2: Nitric Oxide Signalling in Yeast.), Advances in Microbial Physiology, 72, 29-63 (2018)
  4. 那須野亮, 吉川雄樹, 高木博史, 酵母に見出した一酸化窒素（NO）の合成制御機構と生理機能, 化学と生物, 55(9), 617-623 (2017)
  5. 高木博史, 橋田恵介, 渡辺大輔, 那須野亮, 他4名, ロイシンの蓄積に伴いイソアミルアルコールを過剰生産する「泡盛酵母」の変異株の分離と特性解析, 生物工学会誌, 95(2), 72 (2017)
  6. 高木博史, 那須野亮, 酵母に見出した新規な抗酸化酵素「N-アセチルトランスフェラーゼMpr1」, 化学と生物, 53(3), 148-155 (2015)
  7. 那須野亮, 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の構造機能解析, 奈良先端科学技術大学院大学バイオサイエンス研究科博士論文, 全76p (2013)