メンバー紹介

特命助教伊藤 洋一郎 Yoichiro Ito

経歴

1994/03
埼玉大学工学部環境科学工学科 卒業
1996/03
埼玉大学理工学研究科修士課程 修了
2000/03
埼玉大学理工学研究科博士課程 修了
2000/04
大阪大学工学部応用生物工学科 博士研究員
2005/04
ERATO研究員(大阪大学)
2009/04
(株)豊田中央研究所 客員研究員
2014/04
高機能遺伝子デザイン技術研究組合 博士研究員
2016/04
神戸大学大学院 科学技術イノベーション研究科 特命助教

発表論文

  1. Inokuma, K., Bamba, T., Ishii, J., Ito, Y., Hasunuma, T., Kondo, A. (2016) Enhanced cell-surface display and secretory production of cellulolytic enzymes with Saccharomyces cerevisiae Sed1 signal peptide., Biotechnology and Bioengineering, 113(11), 2358-2366
  2. Ito, Y., Kitagawa, T., Yamanishi, M., Katahira, S., Izawa, S., Irie, K., Furutani-Seiki, M., Matsuyama, T. (2016) Enhancement of protein production via the strong DIT1 terminator and two RNA-binding proteins in Saccharomyces cerevisiae, Scientific Reports, 6, 36997
  3. Ito, Y., Yamanishi, M., Ikeuchi, A., Imamura, C., Matsuyama, T. (2015) Combinatorial screening for transgenic yeast with high cellulose activities in combination with a tunable expression system, PLoS ONE, e0144870
  4. Ito, Y., Yamanishi, M., Ikeuchi, A., Matsuyama, T. (2015) A Highly Tunable System for the Simultaneous Expression of Multiple Enzymes in Saccharomyces cervisiae., ACS Synthetic Biol., 4, 12-16
  5. Ito, Y., Yamanishi, M., Ikeuchi, A., Imamura, C., Tokuhiro, K., Kitagawa, T., Matsuyama, T. (2013) Characterization of five terminator regions that increase the protein yield of a transgene in Saccharomyces cerevisiae., J. Biotechnol., 168, 486-492
  6. Yamanishi, M., Ito, Y., Kintaka, R., Imamura, C., Katahira, S., Ikeuchi, A., Moriya, H., Matsuyama, T. (2013) A Genome-Wide Activity Assessment of Terminator Region in Saccharomyces cervisiae Provides a “Terminatome” Toolbox., ACS Synthetic Biol., 2, 337-347
  7. Ito, Y., Ikeuchi, A., Imamura, C. (2013) Advanced evolutionary molecular engineering to produce thermostable cellulase by using a small but efficient library, Protein Eng. Des. Sel., 26(1), 73-79
  8. Suzuki, M., Tanaka, S., Ito, Y., Inoue, M., Sakai, T., Nishigaki, K. (2012) Simple and tunable Förster resonance energy transfer-based bioprobes for high-throughput monitoring of caspase-3 activation in living cells by using flow cytometry, Biochem. Biophys. Acta 1823, 215-226
  9. Matsumoto, Y., Ito, Y., Tsuru, S., Ying, B. W., Yomo, T. (2011) Bacterial Cells Carrying Synthetic Dual-Function Operon Survived Starvation, J. Biomed. Biotechnol, Article ID 489265 doi:10.1155/2011/489265
  10. Shimizu, Y., Tsuru, S., Ito, Y., Ying, B. W., Yomo, T. (2011) Stochastic Switching Induced Adaptation in a Starved Escherichia coli Population, PLoS ONE, 6(9), e23953
  11. Ying, B. W., Ito, Y., Shimidzu, Y., Yomo, T. (2010) Refined method for the genomic integration of complex synthetic circuits, J. Biosci. Bioeng., 110(5), 529-536
  12. Ito, Y., Toyota, H., Kaneko, K., Yomo, T. (2009) How selection affects phenotypic fluctuationMol. Sys. Biol., 5, Article number 264 doi:10.1038/msb.2009.23
  13. Zhang, W., Suzuki, M., Ito, Y., Douglas, K. T. (2005) A Chemically Modified Green-Fluorescent Protein that Responds to Cleavage of an Engineered Disulphide Bond by Fluorescence Resonance Energy Transfer (FRET)-Based Changes, Chem. Lett., 34(6), 766-767
  14. Suzuki, M., Ito, Y., Sakata, I., Sakai, T., Husimi, Y., Douglas, K. T. (2005) Caspase-3 sensitive signaling in vivo in apoptotic HeLa cells by chemically engineered intramolecular fluorescence resonance energy transfer mutants of green fluorescent protein, Biochem. Biophys. Res. Commun., 330, 454-460
  15. Suzuki, M., Ito, Y., Savage, H. E., Husimi, Y,. Douglas, K. T. (2004) Protease-sensitive signalling by chemically engineered intramolecular fluorescent resonance energy transfer mutants of green fluorescent protein, Biochim. Biophys. Acta., 1679, 222-229
  16. Ito, Y., Kawama, T., Urabe, I., Yomo, T. (2004) Evolution of an Arbitrary Sequence in Solubility, J. Mol. Evol., 58, 196-202
  17. Sato, K., Ito, Y., Yomo, T., Kaneko, K. (2003) On the relation between fluctuation and response in biological systems, PNAS, 100(24), 14086-14090
  18. Suzuki, M., Ito, Y., Savage, H. E., Husimi, Y., Douglas, K. T. (2003) Intramolecular Fluorescent Resonance Energy Transfer (FRET) by BODIPY Chemical Modification of Cysteine-engineered Mutants of Green Fluorescent Protein, Chem. Lett., 32(3), 306-307
  19. Ito, Y., Suzuki, M., Husimi, Y. (2000) A T-extended vector using a green fluorescent protein as an indicator, Gene, 245, 59-63
  20. Ito, Y., Suzuki, M., Husimi, Y. (1999) A Novel Mutant of Green Fluorescent Protein with Enhanced Sensitivity for Microanalysis at 488nm Excitation, Biochem. Biophys. Res. Commun., 264, 556-560

総説・解説等

  1. 中村泰之, 伊藤洋一郎, 梅津光央, 石井純, 近藤昭彦, 低分子抗体医薬の開発展望, バイオインダストリー, Vol.34, No.6, 54-62 (2017)