メンバー紹介

TOP メンバー（先端バイオ） 伊藤　洋一郎

特命准教授伊藤　洋一郎 Yoichiro Ito

経歴

1994/03
埼玉大学　工学部　環境科学工学科　卒業
1996/03
埼玉大学　理工学研究科　修士課程　修了
2000/03
埼玉大学　理工学研究科　博士課程　修了
2000/04
大阪大学　工学部　応用生物工学科　博士研究員
2005/04
ERATO研究員（大阪大学）
2009/04
（株）豊田中央研究所　客員研究員
2014/04
高機能遺伝子デザイン技術研究組合　博士研究員
2015/07
神戸大学　大学院　工学研究科　特命助教
2016/04
神戸大学　大学院　科学技術イノベーション研究科　特命助教
2018/07
神戸大学　先端バイオ工学研究センター　兼任
2020/07
高機能遺伝子デザイン技術研究組合　研究員
2021/04
神戸大学　先端バイオ工学研究センター　特命准教授
　　　　　大学院　科学技術イノベーション研究科　特命准教授

発表論文

  1. Asama, R., Tominaga, M., Ito, S., Ito, Y., Takemura, K., Sakuraba, S., Katsurada, K., Fukuda, N., Kondo, A., Ishii, J.(2024/07) Screening of protein-based inhibitors for the intracellular domain of epidermal growth factor receptor by directed evolution using the yeast Gγ recruitment system, Journal of Bioscience and Bioengineering, 138(5), 375-381
  2. Inokuma, K., Miyamoto, S., Morinaga, K., Kobayashi, Y., Kumokita, R., Bamba, T., Ito, Y., Kondo, A., Hasunuma, T. (2023/04) Direct production of 4-hydroxybenzoic acid from cellulose using cellulase-displaying Pichia pastoris, Biotechnology and Bioengineering, 120(4): 1097-1107
  3. Ito, Y., Ishigami, M., Hashiba, N., Nakamura, Y., Terai, G., Hasunuma, T., Ishii, J., Kondo, A. (2022/09) Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris, Microbial Biotechnology, 15(9), 2364-2378
  4. Ito, Y., Ishigami, M., Terai, G., Nakamura, Y., Hashiba, N., Nishi, T., Nakazawa, H., Hasunuma, T., Asai, K., Umetsu, M., Ishii, J., Kondo, A. (2022/06) A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii, Communications Biology, 5, 561
  5. Kumokita, R., Bamba, T., Inokuma, K., Yoshida, T., Ito, Y., Kondo, A., Hasunuma, T. (2022/05) Construction of an ʟ-Tyrosine chassis in Pichia pastoris enhances aromatic secondary metabolites production from glycerol, ACS Synthetic Biology, 11(6), 2098-2107
  6. Nishi, T., Ito, Y., Nakamura, Y., Yamaji, T., Hashiba, N., Tamai, M., Yasohara, Y., Ishii, J.*, Kondo, A. (2022/01) One-step in vivo assembly of multiple DNA fragments and genomic integration in Komagataella phaffii, ACS Synthetic Biology, 11(2), 644-654
  7. Ito, Y., Terai, G., Ishigami, M., Hashiba, N., Nakamura, Y., Bamba, T., Kumokita, R., Hasunuma, T., Asai, K., Ishii, J.*, Kondo, A.* (2020/12) Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression, Nucleic Acids Research, 48(22), 13000-13012
  8. Ito, Y., Watanabe, T., Aikawa, S., Nishi, T., Nishiyama, T., Nakamura, Y., Hasunuma, T., Okubo, Y., Ishii, J., Kondo, A. (2018/11) Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii, FEMS Yeast Research, 18(7), foy074
  9. Nakamura, Y., Nishi, T., Noguchi, R., Ito, Y., Watanabe, T., Nishiyama, T., Aikawa, S., Hasunuma, T., Ishii, J., Okubo, Y., Kondo, A. (2018/07) A stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA, Applied and Environmental Microbiology, 84(15), e02882-17
  10. Inokuma, K., Bamba, T., Ishii, J., Ito, Y., Hasunuma, T., Kondo, A. (2016/11) Enhanced cell-surface display and secretory production of cellulolytic enzymes with Saccharomyces cerevisiae Sed1 signal peptide., Biotechnology and Bioengineering, 113(11), 2358-2366
  11. Ito, Y., Kitagawa, T., Yamanishi, M., Katahira, S., Izawa, S., Irie, K., Furutani-Seiki, M., Matsuyama, T. (2016/11) Enhancement of protein production via the strong DIT1 terminator and two RNA-binding proteins in Saccharomyces cerevisiae, Scientific Reports, 6, 36997
  12. Ito, Y., Yamanishi, M., Ikeuchi, A., Imamura, C., Matsuyama, T. (2015/12) Combinatorial screening for transgenic yeast with high cellulose activities in combination with a tunable expression system, PLoS ONE, e0144870
  13. Ito, Y., Yamanishi, M., Ikeuchi, A., Matsuyama, T. (2015/01) A Highly Tunable System for the Simultaneous Expression of Multiple Enzymes in Saccharomyces cervisiae., ACS Synthetic Biol., 4, 12-16
  14. Ito, Y., Yamanishi, M., Ikeuchi, A., Imamura, C., Tokuhiro, K., Kitagawa, T., Matsuyama, T. (2013/12) Characterization of five terminator regions that increase the protein yield of a transgene in Saccharomyces cerevisiae., J. Biotechnol., 168, 486-492
  15. Yamanishi, M., Ito, Y., Kintaka, R., Imamura, C., Katahira, S., Ikeuchi, A., Moriya, H., Matsuyama, T. (2013/02) A Genome-Wide Activity Assessment of Terminator Region in Saccharomyces cervisiae Provides a “Terminatome” Toolbox., ACS Synthetic Biol., 2, 337-347
  16. Ito, Y., Ikeuchi, A., Imamura, C. (2013/01) Advanced evolutionary molecular engineering to produce thermostable cellulase by using a small but efficient library, Protein Eng. Des. Sel., 26(1), 73-79
  17. Suzuki, M., Tanaka, S., Ito, Y., Inoue, M., Sakai, T., Nishigaki, K. (2012/02) Simple and tunable Förster resonance energy transfer-based bioprobes for high-throughput monitoring of caspase-3 activation in living cells by using flow cytometry, Biochem. Biophys. Acta 1823, 215-226
  18. Matsumoto, Y., Ito, Y., Tsuru, S., Ying, B. W., Yomo, T. (2011/11) Bacterial Cells Carrying Synthetic Dual-Function Operon Survived Starvation, J. Biomed. Biotechnol, Article ID 489265 doi:10.1155/2011/489265
  19. Shimizu, Y., Tsuru, S., Ito, Y., Ying, B. W., Yomo, T. (2011/09) Stochastic Switching Induced Adaptation in a Starved Escherichia coli Population, PLoS ONE, 6(9), e23953
  20. Ying, B.W., Ito, Y., Shimidzu, Y., Yomo, T. (2010/11) Refined method for the genomic integration of complex synthetic circuits, J. Biosci. Bioeng., 110(5), 529-536
  21. Ito, Y., Toyota, H., Kaneko, K., Yomo, T. (2009/04) How selection affects phenotypic fluctuation, Mol. Sys. Biol., 5, 264
  22. Suzuki, M., Ito, Y., Sakata, I., Sakai, T., Husimi, Y., Douglas, K.T. (2005/05) Caspase-3 sensitive signaling in vivo in apoptotic HeLa cells by chemically engineered intramolecular fluorescence resonance energy transfer mutants of green fluorescent protein, Biochem. Biophys. Res. Commun., 330, 454-460
  23. Zhang, W., Suzuki, M., Ito, Y., Douglas, K.T. (2005/04) A Chemically Modified Green-Fluorescent Protein that Responds to Cleavage of an Engineered Disulphide Bond by Fluorescence Resonance Energy Transfer (FRET)-Based Changes, Chem. Lett., 34(6), 766-767
  24. Suzuki, M., Ito, Y., Savage, H.E., Husimi, Y,. Douglas, K.T. (2004/09) Protease-sensitive signalling by chemically engineered intramolecular fluorescent resonance energy transfer mutants of green fluorescent protein, Biochim. Biophys. Acta., 1679, 222-229
  25. Ito, Y., Kawama, T., Urabe, I., Yomo, T. (2004/02) Evolution of an Arbitrary Sequence in Solubility, J. Mol. Evol., 58, 196-202
  26. Sato, K., Ito, Y., Yomo, T., Kaneko, K. (2003/11) On the relation between fluctuation and response in biological systems, PNAS, 100(24), 14086-14090
  27. Suzuki, M., Ito, Y., Savage, H.E., Husimi, Y., Douglas, K.T. (2003/02) Intramolecular Fluorescent Resonance Energy Transfer (FRET) by BODIPY Chemical Modification of Cysteine-engineered Mutants of Green Fluorescent Protein, Chem. Lett., 32(3), 306-307
  28. Ito, Y., Suzuki, M., Husimi, Y. (2000/03) A T-extended vector using a green fluorescent protein as an indicator, Gene, 245, 59-63
  29. Ito, Y., Suzuki, M., Husimi, Y. (1999/10) A Novel Mutant of Green Fluorescent Protein with Enhanced Sensitivity for Microanalysis at 488nm Excitation, Biochem. Biophys. Res. Commun., 264, 556-560

総説・解説等

  1. 伊藤洋一郎, 石井純, 近藤昭彦, ピキア酵母の多重遺伝子欠損による難分泌性抗体タンパク質の生産性向上, Bioscience & Industry, 81(3), 234-236 (2023/05)
  2. 中村泰之, 伊藤洋一郎, 梅津光央, 石井純, 近藤昭彦, 低分子抗体医薬の開発展望, バイオインダストリー, Vol.34, No.6, 54-62 (2017/06)