メンバー紹介

特命准教授秀瀬 涼太 Ryota Hidese

経歴

2005/03
大分大学 工学部 応用化学科 卒業
2005/04
京都大学 化学研究所 研究生 (2006年3月まで)
2008/03
京都大学大学院 農学研究科 修士課程応用生命科学専攻 修了
2009/04
日本学術振興会 特別研究員(研究職) (2011年3月まで)
2010/03
日本学術振興会 優秀若手研究者海外派遣事業(派遣先:ウィスコンシン大学マディソン校)
特別研究員 (2010年9月まで)
2011/03
京都大学大学院 農学研究科 博士後期課程応用生命科学専攻 修了
2011/04
関西学院大学大学院 理工学研究科 生命環境科学研究センター 博士研究員 (2014年3月まで)
2014/04
マックスプランク陸生微生物学研究所 博士研究員 (2015年3月まで)
2015/04
関西学院大学理工学部生命科学科 助教 (2018年3月まで)
2018/04
神戸大学大学院 科学技術イノベーション研究科 特命准教授

発表論文

  1. Hidese, R., Kawato, K., Nakura, Y., Yasukawa, K., Yanagihara, I., Fujiwara, S. (2018) Thermostable DNA helicase improves the sensitivity of digital PCR, Biochemical and Biophysical Research Communications, 495(3), 2189-2194
  2. Okano, H., Baba, M., Kawato, K., Hidese, R., Yanagihara, I., Kojima, K., Takita, T., Fujiwara, S., Yasukawa, K. (2018) High sensitive RNA detection by one-step RT-PCR using the genetically engineered variant of DNA polymerase with reverse transcriptase activity from hyperthermophilies, Journal of Bioscience and Bioengineering, 125(3), 275-281
  3. Ishii, Y., Akasaka, N., Sakoda, H., Hidese, R., Fujiwara, S. (2018) Leucine responsive regulatory protein is involved in methionine metabolism and polyamine homeostasis in acetic acid bacterium Komagataeibacter europaeus, Journal of Bioscience and Bioengineering, 125(1), 67-75
  4. Hidese, R., Tse, KM., Kimura, S., Mizohata, E., Fujita, J., Horai, Y., Umezawa, N., Higuchi, T., Niitsu, M., Oshima, T., Imanaka, T., Inoue, T., Fujiwara, S. (2017) Active site geometry of a novel aminopropyltransferase for biosynthesis of hyperthermophile-specific branched-chain polyamine. The FEBS Journal, 284(21), 3684-3701
  5. Yasukawa, K., Iida, K., Okano, H., Hidese, R., Baba, M., Yanagihara, I., Kojima, K., Takita, T., Fujiwara, S. (2017) Next-generation sequencing-based analysis of reverse transcriptase fidelity. Biochemical and Biophysical Research Communications, 492(2): 147-153
  6. Hidese, R., Yamashita, K., Kawazuma, K., Kanai, T., Atomi, H., Imanaka, T., Fujiwara, S. (2017) Gene regulation of two ferredoxin:NADP+ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis, Extremophiles, 21(5): 903-917
  7. Hidese, R., Im, KH., Kobayashi, M., Niitsu, M., Furuchi, T., Fujiwara, S. (2017) Identification of a novel acetylated form of branched-chain polyamine from a hyperthermophilic archaeon Thermococcus kodakarensis, Bioscience, Biotechnology, and Biochemistry, 81(9): 1845-1849
  8. Okano, H., Baba, M., Yamasaki, T., Hidese, R., Fujiwara, S., Yanagihara, I., Ujiiye, T., Hayashi, T., Kojima, K., Takita, T., Yasukawa, K. (2017) High sensitive one-step RT-PCR using MMLV reverse transcriptase, DNA polymerase with reverse transcriptase activity, and DNA/RNA helicase, Biochemical and Biophysical Research Communications, 487(1): 128-133
  9. Gao, L., Hidese, R., Fujiwara, S. (2017) Function of a thermophilic archaeal chaperonin is enhanced by electrostatic interactions with its targets, Journal of Bioscience and Bioengineering, 124(3): 283-288
  10. Okano, H., Katano, Y., Baba, M., Fujiwara, A., Hidese, R., Fujiwara, S., Yanagihara, I., Hayashi, T., Kojima, K., Takita, T., Yasukawa, K. (2017) Enhanced detection of RNA by MMLV reverse transcriptase coupled with termostable DNA polymerase and DNA/RNA helicase, Enzyme and Microbial Technology, 96: 111-120
  11. Fujiwara, A., Kawato, K., Kato, S., Yasukawa, K., Hidese, R., Fujiwara, S. (2016) Application of a Euryarchaeota-Specific Helicase from Thermococcus kodakarensis for Noise Reduction in PCR, Applied and Environmental Microbiology, 82(10): 3022-3031
  12. Akasaka, N., Astuti, W., Ishii, Y., Hidese, R., Sakoda, H., Fujiwara, S. (2015) Change in the plasmid copy number in acetic acid bacteria in response to growth phase and acetic acid concentration, Journal of Bioscience and Bioengineering, 119(6): 661-668
  13. Hidese, R., Ataka, K., Bill, E., Shima, S. (2015) CuI and H2O2 Inactivate and FeII Inhibits [Fe]-Hydrogenase at very low Concentrations, ChemBioChem, 16(13): 1861-1865
  14. Akasaka, N., Ishii, Y., Hidese, R., Sakoda, H., Fujiwara, S. (2014) Enhanced production of branched-chain amino acids by Gluconacetobacter europaeus with a specific regional deletion in a leucine responsive regulator, Journal of Bioscience and Bioengineering, 118(6): 607-615
  15. Okada, K., Hidese, R., Fukuda, W., Niitsu, M., Takao, K., Horai, Y., Umezawa, N., Higuchi, T., Oshima, T., Yoshikawa, Y., Imanaka, T., Fujiwara, S. (2014) Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles, Journal of Bacteriology, 196(10): 1866-1876
  16. Hidese, R., Nishikawa, R., Gao, L., Katano, M., Imai, T., Kato, S., Kanai, T., Atomi, H., Imanaka, T., Fujiwara, S. (2014) Different roles of two transcription factor B proteins in the hyperthermophilic archaeon Thermococcus kodakarensis, Extremophiles, 18(3): 573-588
  17. Hidese, R., Inoue, T., Imanaka, T., Fujiwara, S. (2014) Cysteine desulphurase plays an important role in environmental adaptation of the hyperthermophilic archaeon Thermococcus kodakarensis, Molecular Microbiology, 93(2): 331-345
  18. Hidese, R., Mihara, H., Kurihara, T., Esaki, N. (2014) Global identification of genes affecting iron-sulfur cluster biogenesis and iron homeostasis, Journal of Bacteriology, 196(6):1238-1249
  19. Nagaoka, E., Hidese, R., Imanaka, T., Fujiwara, S. (2013) Importance and determinants of induction of cold-induced DEAD RNA helicase in the hyperthermophilic archaeon Thermococcus kodakarensis, Journal of Bacteriology, 195(15): 3442-3450
  20. Akasaka, N., Sakoda, H., Hidese, R., Ishii, Y., Fujiwara, S. (2013) An efficient method using Gluconacetobacter europaeus to reduce an unfavorable flavor compound, acetoin, in rice vinegar production, Applied and Environmental Microbiology, 79(23): 7334-7342
  21. Hidese, R., Mihara, H., Kurihara, T., Esaki, N. (2012) Pseudomonas putida PydR, a RutR-like transcriptional regulator, represses the dihydropyrimidine dehydrogenase gene in the pyrimidine reductive catabolic pathway, Journal of Biochemistry, 152(4): 341-346
  22. Hidese, R., Mihara, H., Kurihara, T., Esaki, N. (2011) Escherichia coli dihydropyrimidine dehydrogenase is a novel NAD-dependent heterotetramer essential for the production of 5,6-dihydrouracil, Journal of Bacteriology, 193(4): 989-993
  23. Hidese, R., Mihara, H., Esaki, N. (2011) Bacterial cysteine desulfurases: versatile key players in biosynthetic pathways of sulfur-containing biofactors, Applied Microbiology and Biotechnology, 91(1): 47-61
  24. Hidese, R., Mihara, H., Esaki, N. (2009) Functional analysis of a novel NADH-dependent dihydropyrimidine dehydrogenase from Escherichia coli, Vitamins (Japan), 83(9): 528-532
  25. Mihara, H., Hidese, R., Yamane, M., Kurihara, T., Esaki, N. (2008) The iscS gene deficiency affects the expression of pyrimidine metabolism genes, Biochemical and Biophysical Research Communications, 372(3): 407-411

総説・解説等

  1. 藤原伸介, 藤原綾子, 秀瀬涼太, 耐熱性ヘリカーゼを利用した高精度核酸検出技術の開発, 生物工学会誌, 95(7): 383-387 (2017)
  2. 秀瀬涼太, 進化は長い目で見て. 生物工学会誌, 94(3): 131 (2016)
  3. 秀瀬涼太, 藤原伸介, 超好熱性アーキアThermococcus kodakarensis由来分岐鎖ポリアミン合成酵素の構造と機能, 酵素工学ニュース, 75 (2016)
  4. 秀瀬涼太, 藤原伸介, 好熱菌の低温ストレス耐性, 生物工学会誌, 93(8):464-467 (2015)
  5. 秀瀬涼太, 今岡進, 藤原伸介, 耐熱性タンパク質を利用したビスフェノールAの吸着, ケミカルエンジニアリング, 59(4): 17-21(2014)
  6. 秀瀬涼太, 藤原伸介, 高温において翻訳活性化作用を持つポリアミン, バイオサイエンスとインダストリー, 70(3): 211-212 (2012)
  7. 秀瀬涼太, シャペロニンと生物進化, 生物工学会誌, 90(1): 39 (2012)

著書

  1. Hidese, R., Fukuda, W., Niitsu, M., Fujiwara, S. Identification of branched-chain polyamines in hyperthermophiles, In Methods in Molecular Biology, Ruben Alcazar and Antonio F. Tiburcio (eds.). Heidelberg: Springer, pp. 81-94 (2018)
  2. Fujiwara, S., Hidese, R., Inoue, T., Fukuda, W. Protein synthesis and polyamines in thermophiles: Effect of polyamines on nucleic acid maintenance and gene expression, In Polyamines, a universal molecular nexus for growth, survival and specialized metabolism, Tomonobu Kusano and Hideyuki Suzuki (eds.). Heidelberg: Springer, pp. 143-152 (2015)
  3. Fukuda, W., Hidese, R., Fujiwara, S. Long-chain and branched polyamines in thermophilic microbes. In Polyamines, a universal molecular nexus for growth, survival and specialized metabolism, Tomonobu Kusano and Hideyuki Suzuki (eds.). Heidelberg: Springer, pp. 15-25 (2015)