メンバー紹介

TOP メンバー（先端バイオ） Gregory Guirimand

特命准教授Gregory Guirimand

経歴

2002-2007
B.A in Biology (Plant Physiology) University of Tours (France)
2007-2011
Ph.D., Biology (Plant Physiology) University of Tours (France)
2011-2013
JSPS Foreign Postdoctoral Researcher Osaka University (Japan)
2013-2016
ST Postdoctoral Researche Kobe University
2016/04
神戸大学大学院　科学技術イノベーション研究科　特命助教
2018/07
神戸大学　先端バイオ工学研究センター　兼任
2019/04
神戸大学大学院　科学技術イノベーション研究科　研究員
2019/06
フランス、トゥール大学のBBVラボ、LE STUDIUM研究フェロー
2021/04
フランス、トゥール大学の植物バイオ分子およびバイオテクノロジー（BBV）研究所の招待研究者
2021/11
アラブ首長国連邦アブダビの技術革新研究所（TII-BRC）バイオテクノロジー研究センター、分子バイオテクノロジーおよびゲノミクスの主任研究者
2025/04
神戸大学　先端バイオ工学研究センター　特命准教授

発表論文

  1. Mall, R., Singh, A., Patel, CN., Guirimand, G., Castiglione, F. (2024/06) VISH-Pred: an ensemble of fine-tuned ESM models for protein toxicity prediction. Briefings in Bioinformatics. 25(4):bbae270
  2. St-Pierre, B., Mahroug, S., Guirimand, G., Courdavault, V., Burlat, V. (2022/06) RNA In Situ Hybridization of Paraffin Sections to Characterize the Multicellular Compartmentation of Plant Secondary Metabolisms. Methods in Molecular Biology. 2505:1-32
  3. Bamba, T., Guirimand, G., Kondo, A., Hasunuma, T. (2021/12) Enzyme display technology for lignocellulosic biomass valorization by yeast cell factories. Current Opinion in Green and Sustainable Chemistry. 33: 100584
  4. Koudounas, K., Guirimand, G., Rojas Hoyos, LF., Carqueijeiro, I., Lemos Cruz, P., Stander, E., Kulagina, N., Perrin, J., Oudin, A., Besseau, S., Lanoue, A., Atehortùa, L., St-Pierre, B., Giglioli-Guivarc'h, N., Papon, N., O'Connor, SE., Courdavault, V. (2021/11) Tonoplast and Peroxisome Targeting of γ-tocopherol N-methyltransferase Homologs Involved in the Synthesis of Monoterpene Indole Alkaloids. Plant and Cell Physiology., 63(2), 200-216
  5. Kulagina, N., Guirimand, G., Melin, C., Lemos-Cruz, P., Carqueijeiro, I., De Craene, JO., Oudin, A., Heredia, V., Koudounas, K., Unlubayir, M., Lanoue, A., Imbault, N., St-Pierre, B., Papon, N., Clastre, M., Giglioli-Guivarc'h, N., Marc, J., Besseau, S., Courdavault, V. (2021/07) Enhanced bioproduction of anticancer precursor vindoline by yeast cell factories. Microbial Biotechnology., 14(6), 2693-2699
  6. Yukawa, T., Bamba, T., Guirimand, G., Matsuda, M., Hasunuma, T., Kondo, A. (2020/09) Optimization of 1,2,4-butanetriol production from xylose in Saccharomyces cerevisiae by metabolic engineering of NADH/NADPH balance. Biotechnology and Bioengineering, 118(1), 175-185
  7. Lemos Cruz, P., Kulagina, N., Guirimand, G., De Craene, JO., Besseau, S., Lanoue, A., Oudin, A., Giglioli-Guivarc'h, N., Papon, N., Clastre, M., Courdavault, V. (2021/06) Optimization of Tabersonine Methoxylation to Increase Vindoline Precursor Synthesis in Yeast Cell Factories. Molecules., 26(12),3596
  8. Guirimand, G., Kulagina, N., Papon, N., Hasunumam T., Courdavault, V. (2020/09) Innovative Tools and Strategies for Optimizing Yeast Cell Factories. Trends in Biotechnology., 39(5)
  9. Guirimand, G., Guihur, A., Perello, C., Phillips, M., Mahroug, S., Oudin, A., Dugé de Bernonville, T., Besseau, S., Lanoue, A., Giglioli-Guivarc'h, N., Papon, N., St-Pierre, B., Rodríguez-Concepción, M., Burlat, V., Courdavault, V. (2020/04) Cellular and Subcellular Compartmentation of the 2C-Methyl-D-Erythritol 4-Phosphate Pathway in the Madagascar Periwinkle. Plants., 9(4):462
  10. Bamba, T., Yukawa, T., Guirimand, G., Inokuma, K., Sasaki, K., Hasunuma, T., Kondo, A. (2019/12) Production of 1,2,4-butanetriol from xylose by Saccharomyces cerevisiae through Fe metabolic engineering, Metabolic Engineering, 56: 17-27
  11. Guirimand, GGY., Bamba, T., Matsuda, M., Inokuma, K., Morita, K., Kitada, Y., Kobayashi, Y., Yukawa, T., Sasaki, K., Ogino, C., Hasunuma, T., Kondo, A. (2019/09) Combined cell surface display of β-D-glucosidase (BGL), maltose transporter (MAL11) and overexpression of cytosolic xylose reductase (XR) in Saccharomyces cerevisiae enhance cellobiose/xylose coutilization for xylitol bio-production from lignocellulosic biomass, Biotechnology Journal, 14(9): e1800704 (9月号表紙採用)
  12. Guirimand, G., Inokuma, K., Bamba, T., Matsuda, M., Morita, K., Sasaki, K., Ogino, C., Berrin, JG., Hasunuma, T., Kondo, A. (2019/05) Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass, Green Chemistry, 21, 1795-1808
  13. Guirimand, G., Sasaki, K., Inokuma, K., Bamba T., Hasunuma, T., Kondo, A. (2016) Cell-surface engineering of Saccharomyces cerevisiae combined with membrane separation technology for xylitol production from rice straw hydrolysate, Applied Microbiology and Biotechnology, 100(8), 3477-3487
  14. Besseau, S., Kellner, F., Lanoue, A., Thamm, A., Salim, V., Schneider, B., Geu-Flores, F., Höfer, R., Guirimand, G., Guihur, A., Oudin, A., Glevarec, G., Foureau, E., Papon, N., Clastre, M., Giglioli-Guivarc'h, N., St-Pierre, B., Werck-Reichhart, D., Burlat, V., De Luca, V., O'Connor, S., Courdavault, V. (2013) A pair of tabersonine 16-hydroxylases initiates the synthesis of vindoline in an organ dependent manner in Catharanthus roseus, Plant Physiol, 163: 1792-1803
  15. Héricourt, F., Chefdor, F., Bertheau, L., Tanigawa, M., Maeda, T., Guirimand, G., Courdavault, V., Larcher, M., Depierreux, C., Bénédetti, H., Morabito, D., Brignolas, F., Carpin, S. (2013) Characterization of histidine-aspartate kinase HK1 and identification of histidine phosphotransfer proteins as potential partners in a Populus multistep phosphorelay, Physiol. Plant, 149: 188-199
  16. Simkin, A.J., Miettinen, K., Claudel, P., Burlat, V., Guirimand, G., Courdavault, V., Papon, N., Meyer, S., Godet, S., St-Pierre, B., Giglioli-Guivarc'h, N., Fischer, M.J., Memelink, J., Clastre, M. (2013) Characterization of the plastidial geraniol synthase from Madagascar periwinkle which initiates the monoterpenoid branch of the alkaloid pathway in internal phloem associated parenchyma, Phytochemistry, 85: 36-43
  17. Guirimand, G., Guihur, A., Phillips, M., Oudin, A., Glévarec, G., Mahroug, S., Melin, C., Papon, N., Clastre, M., Giglioli-Guivarc'h, N., St-Pierre, B., Rodríguez-Concepción, M., Burlat, V., Courdavault, V. (2012) Triple subcellular targeting of isopentenyl diphosphate isomerases encoded by a single gene, Plant Signal. Behav., 11: 1495-1497
  18. Bertheau, L., Chefdor, F., Guirimand, G., Courdavault, V., Depierreux, C., Morabito, D., Brignolas, F., Hericourt, F., Carpin, S. (2012) Identification of five B-type response regulators as members of a multistep phosphorelay system interacting with histidine-containing phosphotransfer partners of Populus osmosensor, BMC Plant Biol., 12: 241
  19. Ginis, O., Oudin, A., Guirimand, G., Chebbi, M., Courdavault, V., Glevarec, G., Papon, N., Creche, J., Courtois, M. (2012) A type-B response regulator drives the expression of the hydroxymethylbutenyl diphosphate synthase gene in periwinkle, J. Plant Physiol., 169: 1571-1574
  20. Guirimand, G., Guihur, A., Phillips, M.A., Oudin, A., Glévarec, G., Melin, C., Papon, N., Clastre, M., St-Pierre, B., Rodríguez-Concepción, M., Burlat, V., Courdavault, V. (2012) A single gene encodes isopentenyl diphosphate isomerase isoforms targeted to plastids, mitochondria and peroxisomes in Catharanthus roseus, Plant Mol. Biol., 79: 443-459
  21. Guirimand, G., Simkin, A.J., Papon, N., Besseau, S., Burlat, V., St-Pierre, B., Giglioli-Guivarc'h, N., Clastre, M., Courdavault, V. (2012) Cycloheximide as a tool to investigate protein import in peroxisomes: A case study of the subcellular localization of isoprenoid biosynthetic enzymes, J. Plant Physiol., 169: 825-829
  22. Thabet, I., Guirimand, G., Papon, N., Courdavault, V., Godet, S., Dutilleul, C., Giglioli-Guivarc’h, N., Bouzid, S., Clastre, M., Simkin, A.J. (2011) The subcellular localization of periwinkle farnesyl diphosphate synthase gives insight into the role of peroxisome in isoprenoid biosynthesis, J. Plant Physiol., 168: 2110-2116
  23. Simkin, A., Guirimand, G., Papon, N., Courdavault, V., Thabet, I., Ginis, O., Bouzid, S., Giglioli-Guivarc’h, N., Clastre, M. (2011) Peroxisomal Localisation of the final steps of the Mevalonic Acid Pathway in planta, Planta, 234: 903-914
  24. Thabet, I., Guirimand, G., Guihur, A., Lanoue, A., Courdavault, V., Papon, N., Bouzid, S., Giglioli-Guivarc’h, N., Simkin, A.J., Clastre, M. (2011) Characterization and subcellular localization of geranylgeranyl diphosphate synthase from Catharanthus roseus, Mol. Biol. Rep., 39: 3235-3243
  25. Guirimand, G., Guihur, A., Ginis, O., Poutrain, P., Héricourt, F., Oudin, A., Lanoue, A., St-Pierre, B., Burlat, V., Courdavault, V. (2011) The subcellular organisation of the strictosidine biosynthesis in Catharanthus roseus epidermis highlights several trans-tonoplast translocations of intermediate metabolites, FEBS J., 278: 749-763
  26. Poutrain, P., Guirimand, G., Glévarec, G., Courdavault, V., Pichon, O. (2011) Molecular characterization of an Aux/IAA of Catharanthus roseus, J. Plant Growth Regul., 30: 235-241
  27. Guirimand, G., Guihur, A., Poutrain, P., Héricourt, F., Mahroug, S., St-Pierre, B., Burlat, V., Courdavault, V. (2011) Spatial organization of the vindoline biosynthetic pathway in Catharanthus roseus, J. Plant Physiol., 168: 549-57
  28. Poutrain, P., Guirimand, G., Mahroug, S., Burlat, V., Melin, C., Ginis, O., Oudin, A., Giglioli-Guivarc'h, N., Pichon, O., Courdavault, V. (2011) Molecular cloning and characterisation of two calmodulin isoforms of the Madagascar periwinkle Catharanthus roseus, Plant Biol., 13: 36-41
  29. Guirimand, G., Courdavault, V., Lanoue, A., Mahroug, S., Guihur, A., Blanc, N., Giglioli-Guivarc'h, N., St-Pierre, B., Burlat, V. (2010) Strictosidine activation in Apocynaceae: towards a "nuclear time bomb"?, BMC Plant Biol., 10: 182
  30. Guirimand, G., Burlat, V., Oudin, A., Lanoue, A., St-Pierre, B., Courdavault, V. (2009) Optimization of the transient transformation of Catharanthus roseus cells by particle bombardment and its application to the subcellular localization of hydroxymethylbutenyl 4-diphosphate synthase and geraniol 10-hydroxylase, Plant Cell Rep., 28: 1215-1234

総説・解説等

  1. Zafar, AM., El Khamlichi, C., Shakeel, A., Farooq, MU., Guirimand, G., Hasunuma, T., Wolff, C. (2025) Nanotechnology in Treatment and Bioremediation, In Advances in Green Nanotechnology, Elsevier, (under review)
  2. Kahar, P., Guirimand, G., Hasunuma, T. (2023) Ethanol Production by Recombinant CBP Yeasts, In Handbook of Biorefinery Research and Technology, Springer, 1-22
  3. 番場崇弘, 猪熊健太郎, Gregory Guirimand, 蓮沼誠久, 細胞表層提示酵母を利用したクラフトパルプからのキシリトール生産, バイオサイエンスとインダストリー(B&I), vol.77(5), 390-392 (2019/09)
  4. Guirimand, G., Courdavault, V., St-Pierre, B., Burlat, V. (2010) Biosynthesis and Regulation of Alkaloids. In Plant Developmental Biology - Biotechnological Perspectives: Volume 2, pp. 139-160, E.C. Pua and M.R. Davey eds. Springer-Verlag GmbH Berlin Heidelberg.