メンバー紹介

TOP メンバー（先端バイオ） 番場　崇弘

特命准教授番場　崇弘 Takahiro Bamba

経歴

2012/03
神戸大学　工学部　応用化学科　卒業
2014/03
神戸大学　大学院　工学研究科　応用化学専攻　博士課程前期　修了
2017/04
神戸大学　大学院　科学技術イノベーション研究科　学生研究支援員（研究職）
2018/03
神戸大学　大学院　工学研究科　応用化学専攻　博士課程後期　修了
2018/04
神戸大学　大学院　科学技術イノベーション研究科　学術研究員
2021/03
神戸大学　産官学連携本部　特命助教
2021/04
神戸大学　大学院　科学技術イノベーション研究科　特命助教
2022/04
先端バイオ工学推進機構　研究員
2022/10
神戸大学　先端バイオ工学研究センター　特命助教
　
2025/04
神戸大学　先端バイオ工学研究センター　特命准教授

発表論文

  1. Moreno,D.S., Cunha, J., de Melo, L.D.R., Tanaka, K., Bamba, T., Hasunuma, T., Azeredo, J., Domingues, L. (2025/04) CRISPR-Cas9 engineered Saccharomyces cerevisiae for endolysin delivery to combat Listeria monocytogenes, Applied Microbiology and Biotechnology, 109, 81
  2. Tanaka, K., Yukawa, T., Bamba T., Wakiya, M., Kumokita, R., Jin Y.S., Kondo, A., Hasunuma, T.*(2025/01) Engineering Saccharomyces cerevisiae for growth on xylose using an oxidative pathway, Applied Microbiology and Biotechnology, 109, 30
  3. Bamba, T., Munakata, R., Ushiro, Y., Kumokita, R., Tanaka, S., Hori, Y., Kondo, A., Yazaki, K., Hasunuma, T. (2024/12) De novo production of the bioactive phenylpropanoid artepillin C using membrane-bound prenyltransferase in Komagataella phaffii. ACS Synthetic Biology, 13(12):4040-4049
  4. Bamba, T., Aoki, R., Hori, Y., Ishikawa, S., Yoshida, KI., Taoka, N., Kobayashi, S., Yasueda, H., Kondo, A., Hasunuma, T. (2024/06) High-throughput evaluation of hemolytic activity through precise measurement of colony and hemolytic zone sizes of engineered Bacillus subtilis on blood agar, Biology Methods & Protocols, 9(1):bpae044

  5. Mori, H., Matsui, M., Bamba, T., Hori, Y., Kitamura, S., Toya, Y., Hidese, R., Yasueda, H., Hasunuma, T., Shimizu, H., Taoka, N., Kobayashi, S. (2024/07) Engineering Escherichia coli for efficient glutathione production, Metabolic engineering, 84, 180-190
  6. Tanaka, K., Bamba, T., Kondo, A., Hasunuma, T. (2024/02) Metabolomics-based development of bioproduction processes toward industrial-scale production. Current Opinion in Biotechnology, 85, 103057
  7. Kumokita, R., Bamba, T., Yasueda, H., Tsukida, A., Nakagawa, K., Kitagawa, T., Yoshioka, T., Matsuyama, H., Yamamoto, Y., Maruyama, S., Hayashi, T., Kondo, A., Hasunuma,T. (2024/02) High-level phenol bioproduction by engineered Pichia pastoris in glycerol fed-batch fermentation using an efficient pertraction system, Bioresource Technology, 393, 130144
  8. Yanagibashi, S., Bamba, T., Kirisako, T., Kondo, A., Hasunuma, T. (2024/01) The potency of mitochondria enlargement for mitochondria-mediated terpenoid production in yeast, Applied Microbiology and Biotechnology, 108: 110
  9. Yanagibashi, S., Bamba, T., Kirisako, T., Kondo, A., Hasunuma, T. (2024/01) Beneficial effect of optimizing the expression balance of the mevalonate pathway introduced into the mitochondria on terpenoid production in Saccharomyces cerevisiae, Journal of Bioscience and Bioengineering, 137(1): 16-23
  10. Bamba, T., Hori, Y., Umebayashi, K., Soh, C., Hakozaki, T., Toyama, K., Osumi, M., Kondo, A., Hasunuma, T. (2023/11) Comprehensive metabolic profiling of Geotrichum candidum and comparison with Saccharomyces cerevisiae, Journal of Bioscience and Bioengineering, 137(1): 9-15
  11. Inokuma, K., Miyamoto, S., Morinaga, K., Kobayashi, Y., Kumokita, R., Bamba, T., Ito, Y., Kondo, A., Hasunuma, T. (2023/04) Direct production of 4-hydroxybenzoic acid from cellulose using cellulase-displaying Pichia pastoris, Biotechnology and Bioengineering, 120(4): 1097-1107
  12. Yukawa, T., Bamba, T., Matsuda, M., Yoshida, T., Inokuma, K., Kim, J., Lee. J.W., Jin, Y.S., Kondo, A., Hasunuma, T. (2023/02) Enhanced production of 3,4-dihydroxybutyrate from xylose by engineered yeast via xylonate re-assimilation under alkaline condition, Biotechnology and Bioengineering, 120(2): 511-523
  13. Kumokita, R., Bamba, T., Inokuma, K., Yoshida, T., Ito, Y., Kondo, A., Hasunuma, T. (2022/05) Construction of an ʟ-Tyrosine chassis in Pichia pastoris enhances aromatic secondary metabolites production from glycerol, ACS Synthetic Biology, 11(6), 2098-2107
  14. Bamba, T.*, Guirimand, G.*, Kondo, A., Hasunuma, T. (2022/02) Enzyme display technology for lignocellulosic biomass valorization by yeast cell factories, Current Opinion in Green and Sustainable Chemistry, 33: 100584
  15. Inokuma, K., Kitada, Y., Bamba, T., Kobayashi, Y., Yukawa, T., den Haan, R., van Zyl, W. H., Kondo, A., Hasunuma, T. (2021/08) Improving the functionality of surface-engineered yeast cells by altering the cell wall morphology of the host strain, Applied Microbiology and Biotechnology ,105(14-15): 5895-5904
  16. Takenaka, M., Yoshida, T., Hori, Y., Bamba, T., Mochizuki, M., Vavricka, CJ., Hattori, T., Hayakawa, Y., Hasunuma, T.*, Kondo, A. (2021/01) An ion-pair free LC-MS/MS method for quantitative metabolite profiling of microbial bioproduction systems, Talanta, 222, 121625
  17. Yukawa, T., Bamba, T., Guirimand, G., Matsuda, M., Hasunuma, T.*, Kondo, A*. (2021/01) Optimization of 1,2,4-butanetriol production from xylose in Saccharomyces cerevisiae by metabolic engineering of NADH/NADPH balance, Biotechnology and Bioengineering, 118(1), 175-185
  18. Ito, Y., Terai, G., Ishigami, M., Hashiba, N., Nakamura, Y., Bamba, T., Kumokita, R., Hasunuma, T., Asai, K., Ishii, J.*, Kondo, A.* (2020/12) Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression, Nucleic Acids Research, 48(22), 13000-13012
  19. Bamba, T., Yukawa, T., Guirimand, G., Inokuma, K., Sasaki, K., Hasunuma, T., Kondo, A. (2019/12) Production of 1,2,4-butanetriol from xylose by Saccharomyces cerevisiae through Fe metabolic engineering, Metabolic Engineering, 56: 17-27
  20. Guirimand, GGY., Bamba, T., Matsuda, M., Inokuma, K., Morita, K., Kitada, Y., Kobayashi, Y., Yukawa, T., Sasaki, K., Ogino, C., Hasunuma, T., Kondo, A. (2019/09) Combined cell surface display of β-D-glucosidase (BGL), maltose transporter (MAL11) and overexpression of cytosolic xylose reductase (XR) in Saccharomyces cerevisiae enhance cellobiose/xylose coutilization for xylitol bio-production from lignocellulosic biomass, Biotechnology Journal, 14(9): e1800704 (9月号表紙採用)
  21. Guirimand, G., Inokuma, K., Bamba, T., Matsuda, M., Morita, K., Sasaki, K., Ogino, C., Berrin, JG., Hasunuma, T., Kondo, A. (2019/05) Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass, Green Chemistry, 21, 1795-1808
  22. Kobayashi, J., Sasaki, D., Bamba, T., Hasunuma, T., Kondo, A. (2019/02) Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae, Applied Microbiology and Biotechnology, 103(3), 1243-1254
  23. Bamba, T., Inokuma, K., Hasunuma, T., Kondo, A. (2018/03) Enhanced cell-surface display of a heterologous protein using SED1 anchoring system in SED1-disrupted Saccharomyces cerevisiae strain, Journal of Bioscience and Bioengineering, 125(3), 306-310
  24. Inokuma, K, Iwamoto, R., Bamba, T., Hasunuma, T., Kondo, A. (2017/12) Improvement of xylose fermentation ability under heat and acid co-stress in Saccharomyces cerevisiae using genome shuffling technique, Frontiers in Bioengineering and Biotechnology, 5: 81
  25. Mert, MJ., Rose, SH., la Grange, DC., Bamba, T., Hasunuma, T., Kondo, A., van Zyl, WH. (2017/10) Quantitative metabolomics of a xylose‑utilizing Saccharomyces cerevisiae strain expressing the Bacteroides thetaiotaomicron xylose isomerase on glucose and xylose, Journal of Industrial Microbiology and Biotechnology, 44(10), 1459-1470
  26. Inokuma, K., Bamba, T., Ishii, J., Ito, Y., Hasunuma, T., Kondo, A. (2016/11) Enhanced cell-surface display and secretory production of cellulolytic enzymes with Saccharomyces cerevisiae Sed1 signal peptide, Biotechnology and Bioengneering, 113(11): 2358-2366
  27. Guirimand, G., Sasaki, K., Inokuma, K., Bamba, T., Hasunuma, T., Kondo, A. (2016/04) Cell surface engineering of Saccharomyces cerevisiae combined with membrane separation technology for xylitol production from rice straw hydrolysate, Applied Microbiology Biotechnology, 100(8): 3477-3487
  28. Bamba, T., Hasunuma, T., Kondo, A. (2016/03) Disruption of PHO13 improves ethanol production via the xylose isomerase pathway, AMB Express, 6(1): 4

総説・解説等

  1. 番場崇弘, 棟方涼介, 矢﨑一史, 蓮沼誠久 プロポリス生理活性成分アルテピリンCの酵母による生産, バイオサイエンスとインダストリー(B&I), vol.83(3), 246-247 (2025/05)
  2. 蓮沼誠久, 秀瀬涼太, 番場崇弘, [特集]「生物機能を利用したモノづくり」に貢献するプロセス強化　微生物の高速育種を実現するスマートセル創出プラットフォーム, 化学工学, 86(4), 157-160 (表紙採用) (2022/04)
  3. 番場崇弘, 湯川貴弘, 蓮沼誠久, 鉄代謝改変酵母による1,2,4-ブタントリオール生産, バイオサイエンスとインダストリー(B&I), vol.78(2), 114-115 (2020/03)
  4. 番場崇弘, 猪熊健太郎, Gregory Guirimand, 蓮沼誠久, 細胞表層提示酵母を利用したクラフトパルプからのキシリトール生産, バイオサイエンスとインダストリー(B&I), vol.77(5), 390-392 (2019/09)