1.1 Open image

1.2 Extract chromosomal regions

1.3 Separate close together chromosomes (This part of the process can be omitted.)

1.4 Extract signal regions (This part of the process can be omitted.)

• Change type

1.5 Add ID
1.6 Sort (and normalize)
1.7 Rotate chromosome

• Change LUT

1.8 Detect centromere

1.9 Index labeling (This part of the process can be omitted.)

with Step2-3

1.10 The measurement of the CP or FP

2.1 Average the profile (“AVG” tool)
2.2 Align all colorgrams of the chromosomes (“Align” tool)

3.1 Create an idiogram