Hideyuki Mukai To Japanese
ACADEMIC APPOINTMENT
Associate Professor
Biosignal Research Center, Kobe University
Graduate School of Medicine, Kobe University
PRESENT ADDRESS
Biosignal Research Center, Kobe University,
1-1 Rokkodai, Nada, Kobe 657-8501, Japan
Phone: +81-78-803-6467
E-mail: mukinase@kobe-u.ac.jp
WEB SITE LINKS
Bioupdate (administrator of the web site)
Biobookmark (administrator of the web site)
PROFESSIONAL EDUCATION
Ph.D., Department of Pharmacology, Kobe University,
School of Medicine
M.D., Kobe University, School of Medicine
PUBLICATIONS
H. Mori, H. Mukai, and S. Kitamura
Study on glucose tolerance using a mathematical model with a derivative
insulin secretory mechanism - A kinetic analysis of glucose and insulin
curves during intravenous glucose tolerance tests
MEDINFO 86, Elsevier Science Publishers B. V. (North-Holland), IFIP-IMIA
(1986)
T. Kuno, T. Takeda, M. Hirai, A. Ito, H. Mukai and C. Tanaka
Evidence for a second isoform of the catalytic subunit of
calmodulin-dependent protein phosphatase (calcineurin A).
Biochem. Biophys. Res. Commun. 165, 1352-1358 (1989)
H. Mukai, A. Ito, K. Kishima, T. Kuno and C. Tanaka
3)Calmodulin antagonists differentiate between Ni2+- and Mn2+-stimulated
phosphatase activity of calcineurin.
J. Biochem. 110, 402-406 (1991)
H. Mukai, C.-D. Chang, H. Tanaka, A. Ito, T. Kuno, and C. Tanaka
cDNA cloning of a novel testis-specific calcineurin B-like protein.
Biochem. Biophys. Res. Commun. 179, 1325-1330 (1991)
T. Kuno, H. Tanaka, H. Mukai, C.-D. Chang., K. Hiraga, T.
Miyakawa, and C. Tanaka
cDNA cloning of a calcineurin B homolog in Saccharomyces cerevisiae.
Biochem. Biophys. Res. Commun. 180, 1159-1163 (1991)
T. Kuno, H. Mukai, A. Ito, C.-D. Chang, K. Kishima, N. Saito,
and C. Tanaka
Distinct cellular expression of calcineurin A a and A b in rat brain.
J. Neurochem. 58, 1643-1651 (1992)
T. Kuno, Y. Kajimoto, T. Hashimoto, H. Mukai, Y. Shirai, S.
Saheki, and C. Tanaka
cDNA cloning of a neural visinin-like Ca2+-binding protein.
Biochem. Biophys. Res. Commun. 184, 1219-1225 (1992)
C.-D. Chang, T. Takeda, H. Mukai, H. Shuntoh, T. Kuno, and C.
Tanaka
Molecular cloning and characterization of the promotor region of the
calcineurin A a gene.
Biochem. J. 288, 801-805 (1992)
T. Nakamura, H. Tsutsumi, H. Mukai, T. Kuno and T. Miyakawa
Ca2+/calmodulin-activated protein phosphatase (PP2B) of Saccharomyces
cerevisiae; PP2B activity is not essential for growth.
FEBS lett. 309, 103-106 (1992)
H. Mukai, T. Kuno, C.-D. Chang, B. Lane, J. R. Luly, and C.
Tanaka
FKBP12-FK506 complex inhibits phosphatase activity of two mammalian
isoforms of calcineurin irrespective of their substrates or activation
mechanisms.
J.Biochem. 113, 292-298 (1993)
H. Mukai, T. Kuno, H. Tanaka, D. Hirata, T. Miyakawa, and C.
Tanaka
Isolation and characterization of SSE1 and SSE2, new members of the
yeast HSP70 multigene family.
Gene, 132, 57-66 (1993)
M. Asami, T. Kuno, H. Mukai, and C. Tanaka
Detection of the FK506-FKBP-Calcineurin complex by a simple binding
assay.
Biochem. Biophys. Res. Comm. 192, 1388-1394 (1993)
Y.kajimoto, Y. Shirai, H. Mukai, T. Kuno, and C. Tanaka
Molecular cloning of two additional members of the neural visinin-like
Ca2+-binding protein gene family.
J. Neurochem. 61, 1091-1096 (1993)
C.-D. Chang, H. Mukai, M. Asami, T. Kuno, and C. Tanaka
cDNA cloning of an alternatively spliced isoform of the regulatory
subunit of Ca2+/calmodulin-dependent protein phosphatase (calcineurin
Ba2).
Biochim.Biophys.Acta.1217, 174-180. (1994)
H. Mukai, and Y. Ono
A novel protein kinase with leucine zipper-like sequences: its catalytic
domain is highly homologous to that of protein kinase C.
Biochem. Biophys. Res. Comm.199, 897-904. (1994)
H. Mukai, H. Shuntoh, C. -D. Chang, M. Asami, M. Ueno, K. Suzuki,
and T. Kuno
Isolation and characterization of CAJ1, a novel yeast homolog of dnaJ.
Gene 145, 125-127. (1994)
H. Mukai, M. Kitagawa, H. Shibata, H. Takanaga, K, Mori, M.
Shimakawa, M. Miyahara, K, Hirao, and Y, Ono
Activation of PKN, a novel 120-kDa protein kinase with leucine
zipper-like sequences by unsaturated fatty acids and by limited
proteolysis.
Biochem.Biophys.Res.Comm. 204, 348-356 (1994)
H. Mukai, M. Mori, H. Takanaga, M. Kitagawa, H. Shibata, M.
Shimakawa, M. Miyahara, and Y. Ono
Xenopus PKN: cloning and sequencing of the cDNA and identification of
conserved domains.
Biochim. Biophys. Acta.1261, 296-300 (1995)
M. Kitagawa, H. Mukai, H. Shibata, and Y. Ono
Purification and characterization of a novel fatty acid-activated
protein kinase (PKN) from rat testis.
Biochem. J. 310, 657-664 (1995)
H. Takanaga, H. Mukai, M. Shimakawa, H. Konishi, U. Kikkawa,
T.Koizumi, and Y. Ono
Functional characterization of the promotor region of the mouse protein
kinase C g gene.
FEBS Lett. 368, 276-278 (1995)
H. Mukai, M.Miyahara, H. Takanaga, M. Kitagawa, H. Shibata, M.
Shimakawa, and Y. Ono
Identification of psk1+, encoding a novel putative serine/threonine
protein kinase of Schizosaccharomyces pombe, whose mutation conferred
resistance to phenylarsine oxide.
Gene, 166, 155-159 (1995)
T. Kuno, and H. Mukai
Purification and activation of cyclic GMP-dependent protein kinase
Methods in Molecular Biology Vol 41; Signal Transduction Protocols;
Humana Press Inc. (1995)
M. Amano, H. Mukai, Y. Ono, K. Chihara, T. Matsui, Y. Hamajima,
K. Okawa, A. Iwamatsu, K. Kaibuchi.
Identification of a putative target for Rho as a serine-threonine kinase
protein kinase N.
Science, 271, 648-650 (1996)
G. Watanabe, Y. Saito, P. Madaule, T. Ishizaki, K. Fujisawa, N. Morii, H.
Mukai, Y. Ono, A. Kakizuka, S. Narumiya.
Protein kinase N (PKN) and PKN-related protein rhophilin as targets of
small GTPase Rho.
Science, 271, 645-648 (1996)
H. Mukai, M. Toshimori, H. Shibata, M. Kitagawa, M. Shimakawa,
M. Miyahara, H. Sunakawa, and Y. Ono.
PKN associates and phosphorylates the head-rod domain of neurofilament
protein.
J. Biol. Chem. 271, 9816-9822 (1996)
M. Kitagawa, H. Shibata, M. Toshimori, H. Mukai, and Y. Ono.
The role of the unique motifs in the amino-terminal region of PKN on its
enzymatic activity.
Biochem.Biophys.Res.Comm. 220, 963-968 (1996)
H. Shibata, H. Mukai, Y. Inagaki, Y. Homma, K. Kimura, K.
Kaibuchi, S. Narumiya, and Y. Ono.
Characterization of the interaction between RhoA and amino-terminal
region of PKN.
FEBS Lett. 385 (1996) 221-224
H. Mukai, M. Miyahara, H.Sunakawa, H. Shibata, M. Toshimori, M.
Kitagawa, M. Shimakawa, H. Takanaga, and Y. Ono.
Translocation of PKN from the cytosol to the nucleus induced by
stresses.
Proc. Natl. Acad. Sci. USA 93 (1996) 10195-10199
H. Mukai, M. Toshimori, H. Shibata, H. Takanaga, M. Kitagawa, M.
Shimakawa, M. Miyahara, H. Sunakawa, and Y. Ono.
Interaction of PKN with a-actinin
J. Biol. Chem. 272 (1997) 4740-4746
M. Kitagawa, H. Mukai, and Y. Ono
Molecular cloning and characterization of a novel mitochondrial
phosphoprotein, MIPP65, from rat liver
Exp. Cell Res. 235 (1997) 71-78
K. Matuszawa, H. Kosako, N. Inagaki, H. Shibata, H. Mukai, Y.
Ono, M. Amano, K. Kaibuchi, Y. Matsuura, I. Azuma, M. Inagaki
Domain-specific phosphorylation of vimentin and glial fibrillary acidic
protein by PKN
Biochem. Biophys. Res. Comm. 234, 621-625 (1997)
H. Takanaga, H. Mukai, H. Shibata, M. Toshimori, and Y. Ono.
PKN interacts with a paraneoplastic cerebellar degeneration-associated
antigen, which is a potential transcription factor.
Exp. Cell Res. 241, 363-372 (1998)
J. W. Bartch, H. Mukai, N. Takahashi, M. Ronsiek, S. Fuchs, H.
Jockusch, and Y. Ono.
The PKN gene (PRKCL1/Prkcl1) maps to human chromosome 19p13.1 and mouse
chromosome 8 with close linkage to the myodystrophy (myd) mutation.
Genomics 49, 129-132 (1998)
M. L. Standaert, G. Bandyopadhyay, L. Galloway, H. Mukai, Y.
Ono, and R. V. Farese.
Comparative effects of GTPgS and insulin on the activation of Rho,
PI3-kinase and PKN in rat adipocytes. Relationship to glucose transport.
J. Biol. Chem. 273, 7470-7477 (1998)
T. Kawamata, T. Taniguchi, H. Mukai, M. Kitagawa, T. Hashimoto,
K. Maeda, Y. Ono, and C. Tanaka
PKN accumulates in Alzheimer neurofibrillary tangles and associated
endoplasmic reticulum-derived vesicles and phosphorylates tau protein
J. Neuroscience 18, 7402-7410 (1998)
M. Takahashi, H. Mukai, M. Toshimori, M. Miyamoto, Y. Ono
Proteolytic activation of PKN by caspase 3 or related proteases during
apoptosis
Proc. Natl. Acad. Sci. USA 95, 11566-11571 (1998)
T. Hashimoto, H. Mukai, T. Kawamata, T. Taniguchi, Y. Ono, C.
Tanaka
Localization of PKN mRNA in the rat brain.
Mol.Brain.Res. (1998) 59: 143-153
M. Kitagawa, H. Mukai, M. Takahashi, Y. Ono
The role of PKN in the regulation of aB-crystallin expression via heat
shock transcription factor 1
Biochem. Biophys. Res. Comm. (1998) 252: 561-565
H. Mukai, Y. Ono
Protein kinase PKN
Seikagaku. (1998) 70: 1335-9
H. Mukai, Y. Ono
Structure and function of PKN
Tanpakushitsu-Kakusan-Koso. (1998) 43: 1659-65
M. Yamazaki, H. Watanabe, T. Yokozeki, S. Ohno, K. Kaibuchi, H. Shibata,
H. Mukai, Y. Ono, and Y. Kanaho
Identification of the RhoA-binding domain of phospholipase D1.
J. Biol. Chem. (1999) 274: 6035-6038
M. Takahashi, H. Shibata, M. Shimakawa, M. Miyamoto, H. Mukai,
and Y. Ono.
Characterization of a novel giant scaffolding protein, CG-NAP, that
anchors multiple signaling enzymes to centrosome and the Golgi
apparatus.
J. Biol. Chem. (1999) 274: 17267-17274
C. Yoshinaga, H. Mukai, M. Toshimori, M. Miyamoto, Y. Ono
Mutational Analysis of the Regulatory Mechanism of PKN: The Regulatory
Region of PKN Contains an Arachidonic Acid-Sensitive Autoinhibitory
Domain
J.Biochem. (1999) 126:475-84
K. Oishi, H. Mukai, H. Shibata, M. Takahashi, Y. Ono
Identification and characterization of PKN b, a novel isoform of protein
kinase PKN
Biochem.Biophys.Res.Comm.(1999) 261: 808-14
Shibata H, Oda H, Mukai H, Oishi K, Misaki K, Ohkubo H, Ono Y
Interaction of PKN with a neuron-specific basic helix-loop-helix
transcription factor, NDRF/NeuroD2.
Brain Res Mol Brain Res. (1999) 74:126-34
H. Mukai and Y. Ono
C1 and C2 domains
Jikken-igaku. (2000) 18: 29-36
Q. Gao, A.Kumar, S.Srinivasan, L.Singh, H. Mukai, Y. Ono, D. E.
Wazer, and V.Band.
PKN Binds and Phosphorylates Human Papillomavirus E6 Oncoprotein.
J. Biol. Chem (2000)
L. Q. Dong, L. R. Landa, M. J. Wick, L. Zhu, H. Mukai, Y. Ono,
and F. Liu
Phosphorylation of PKN by PDK1 mediates insulin signals to the actin
cytoskelton
Proc. Natl. Acad. Sci. USA (2000)
T. Isagawa, H. Mukai, K. Oishi, T. Taniguchi, H. Hasegawa, T.
Kawamata, C. Tanaka, and Y. Ono
Dual effects of PKN and protein kinase C on phosphorylation of tau
protein by glycogen synthase kinase-3b
Biochem. Biophys. Res. Comm. (2000)
Taniguchi T, Kawamata T, Mukai H, Hasegawa H, Isagawa T, Yasuda
M, Hashimoto T, Terashima A, Nakai M, Ono Y, Tanaka C.
Phosphorylation of tau is regulated by PKN.
J Biol Chem. 2000 Dec 4
Takahashi M, Mukai H, Oishi K, Isagawa T, Ono Y.
Association of immature hypophosphorylated protein kinase cepsilon with
an anchoring protein CG-NAP
J Biol Chem. 2000 Nov 3;275(44):34592-6.
Misaki K, Mukai H, Yoshinaga C, Oishi K, Isagawa T, Takahashi M,
Ohsumi K, Kishimoto T, Ono Y.
PKN delays mitotic timing by inhibition of Cdc25C: Possible involvement
of PKN in the regulation of cell division.
Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):125-129.
Kumiko Oishi, Mikiko Takahashi, Hideyuki Mukai, Yoshiko Banno,
Shigeru Nakashima, Yasunori Kanaho, Yoshinori Nozawa, and Yoshitaka
Ono
PKN Regulates Phospholipase D1 through Direct Interaction
J. Biol. Chem. 2001 276: 18096-18101
Masumi Eto, Toshio Kitazawa, Michio Yazawa, Hideyuki Mukai,
Yoshitaka Ono, and David L. Brautigan
Histamine-induced vasoconstriction involves phosphorylation of a
specific inhibitor protein for myosin phosphatase, by protein kinase C
alpha and delta isoforms
J Biol Chem. 2001 Aug 3;276(31):29072-8.
Shibata H, Oishi K, Yamagiwa A, Matsumoto M, Mukai H, Ono
Y.
PKNbeta interacts with the SH3 Domains of Graf and a Novel Graf Related
Protein, Graf 2, Which Are GTPase Activating Proteins for Rho Family.
J Biochem. 2001 Jul;130(1):23-31.
Hasegawa H, Nakai M, Tanimukai S, Taniguchi T, Terashima A, Kawamata T,
Fukunaga K, Miyamoto E, Misaki K, Mukai H, Tanaka C.
Microglial signaling by amyloid beta protein through mitogen-activated
protein kinase mediating phosphorylation of MARCKS.
Neuroreport. 2001 Aug 8;12(11):2567-71.
Mukai, H. and Ono,Y.
PKN protein kinase
Encyclopedia of molecular medicine, 5 Vol, (2002), by John Wiley &
Sons, Inc.
Takahashi, M., Yamagiwa, A., Nishimura, T., Mukai, H., and Ono,
Y.
"Centrosomal Proteins CG-NAP and Kendrin Provide Microtubule Nucleation Sites by Anchoring gamma-Tubulin Ring Complex"
Mol.Biol.Cell 13, 3235-3245 (2002)
Mukai, H.
"The Structure and Function of PKN, a Protein Kinase Having a Catalytic Domain Homologous to That of PKC"
J.Biochem. 133, 17-27 (2003)
Takahashi, M., Gotoh, Y., Isagawa, T., Nishimura, T., Goyama, E., Kim,
H. S.,
Mukai, H., and Ono, Y.
"Regulation of a Mitogen-Activated Protein Kinase Kinase Kinase, MLTK by PKN"
J.Biochem. 133, 181-187 (2003)
Mukai, H. and Ono, Y.
"Expression and purification of protein kinase C from insect cells"
Methods Mol.Biol. 233, 21-34 (2003)
Gotoh Y, Oishi K, Shibata H, Yamagiwa A, Isagawa T, Nishimura T, Goyama
E, Takahashi M, Mukai H, Ono Y.
Protein kinase PKN1 associates with TRAF2 and is involved in
TRAF2-NF-kappaB signaling pathway.
Biochem Biophys Res Commun. 2004 Feb 13;314(3):688-94.
Iwashita S, Fujii M, Mukai H, Ono Y, Miyamoto M.
Lbc proto-oncogene product binds to and could be negatively regulated by
metastasis suppressor nm23-H2.
Biochem Biophys Res Commun. 2004 Aug 6;320(4):1063-8.
Nishimura T, Takahashi M, Kim HS, Mukai H, Ono Y.
Centrosome-targeting region of CG-NAP causes centrosome amplification by
recruiting cyclin E-cdk2 complex.
Genes Cells. 2005 Jan;10(1):75-86.
Sio, S. O., Suehiro,
T., Sugiura, R., Takeuchi, M., Mukai, H., and Kuno, T.
"The role of the regulatory subunit of fission yeast calcineurin for in vivo activity and its relevance to FK506 sensitivity"
J.Biol.Chem. 280,
12231-12238 (2005)
Isagawa, T.,
Takahashi, M., Kato, T., Jr., Mukai, H., and Ono, Y.
"Involvement of protein kinase PKN1 in G2/M delay caused by arsenite"
Mol.Carcinog. 43, 1-12 (2005)
Mukai, H.,
Isagawa, T., Goyama, E., Tanaka, S., Bence, N. F., Tamura, A., Ono, Y.,
and Kopito, R. R. "Formation of morphologically similar globular
aggregates from diverse aggregation-prone proteins in mammalian cells"
Proc.Natl.Acad.Sci.U.S.A 102, 10887-10892 (2005)
Mukai, H. and Ono, Y.
"Purification and Kinase assay of PKN"
Methods Enzymol 1 Jan 2006 406: p. 234.
Elsevier/Academic Press (2005)
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