メンバー紹介

特命准教授若井 暁 Satoshi Wakai

経歴

2000/03
岡山大学 農学部 総合農業科学科 卒業
2002/03
岡山大学大学院 自然科学研究科 生物資源科学専攻 博士前期課程 修了
2005/09
岡山大学大学院 自然科学研究科エネルギー転換科学専攻博士後期課程 修了 博士(学術)
2005/10
(独)製品評価技術基盤機構 バイオテクノロジー本部 研究職員
2009/04
(独)製品評価技術基盤機構 バイオテクノロジー本部 客員研究員
2009/04
広島大学大学院 生物圏科学研究科 研究員
2013/01
神戸大学 自然科学系先端融合研究環 重点研究部 特命助教
2016/04
神戸大学大学院 科学技術イノベーション研究科 特命准教授

受賞等

  • 2018/03 日本農芸化学会2018年度大会 農芸化学奨励賞 「極限環境微生物のエネルギー変換に関する生化学および熱力学的解析」
  • 2017/05 腐食防食学会2017年度春期講演大会 腐食防食学会進歩賞 「遺伝子解析を用いた微生物腐食診断技術の開発」
  • 2017/03 日本農芸化学会2017年度大会 トピックス賞 「遺伝子組み換え麹菌培養上清カクテルを用いた希少糖高生産プロセスの開発」
  • 2015/11 第15回糸状菌分子生物学コンファレンス 企業特別賞(フジワラテ クノアート特別賞)「コピー数多形とプロモーター強度を加味した黄麹菌での マルチ遺伝子発現」
  • 2014/11 第15回極限環境生物学会 研究奨励賞 「極限環境微生物のエネルギー代謝に関する研究」
  • 2014/03 日本農芸化学会2014年度大会 トピックス賞 「セルロース系バイオマスからの物質生産を可能にしたスーパー麹菌の開発」
  • 2013/03 日本農芸化学会2013年度大会 トピックス賞 「原油試料からの鉄腐食性硝酸塩還元菌の分離」
  • 2012/12/01 第13回極限環境生物学会年会 ポスター賞
  • 2011/01 2010年度農芸化学会中四国支部奨励賞 「無機物と微生物の相互関係に関する研究」
  • 2008/11/07 第11回ヨウ素学会シンポジウム ポスター賞

発表論文

  1. Fujii, S., Masanari-Fujii, M., Kobayashi, S., Kato, C., Nishiyama, M., Harada, Y., Wakai, S., Sambongi, Y. (2018) Commonly stabilized cytochromes c from deep-sea Shewanella and Pseudomonas, Bioscience, Biotechnology, and Biochemistry, 82, 792-799
  2. Aikawa, S., Inokuma, K., Wakai, S., Sasaki, K., Ogino, C., Chang, JS, Hasunuma, T., Kondo, A. (2018) Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition, Biotechnology for Biofuels, 11: 50
  3. Yamane-Koshizawa, D., Fujii, S., Maruno, T., Kobayashi, Y., Yamanaka, M., Wakai, S., Sambongi, Y. (2018) Stabilization of mesophilic Allochromatium vinosum cytochrome c' through specific mutations modeled by a thermophilic homologue, Bioscience, Biotechnology, and Biochemistry, 82, 304-311
  4. Wakai, S., Arazoe, T., Ogino, C., Kondo, A. (Review)(2017) Future insights in fungal metabolic engineering. Bioresource Technology, 245, 1314-1326
  5. Kobayashi, S., Fujii, S., Koga, A., Wakai, S., Matsubayasi, N., Sambongi, Y. (2017) Pseudomonas aeruginosa cytochrome c551 denaturation by five systematic urea derivatives that differ in the alkyl chain length. Bioscience, Biotechnology, and Biochemistry., 81: 1274-1278
  6. Wakai, S., Abe, A., Fujii, S., Nakasone, K., Sambongi, Y. (2017) Pyrophosphate hydrolysis in the extremely halophilic archaeon Haloarcula japonica is catalyzed by a single enzyme with a broad ionic strength range. Extremophiles, 21: 471-477
  7. Fujii, S., Oki, H., Kawahara, K., Yamane, S., Yamanaka, M., Maruno, T., Kobayashi, Y., Masanari, M., Wakai, S., Nishihara, H., Ohkubo, T., Sambongi, Y. (2017) Structural and functional insights into thermally stable cytochrome c' from a thermophile. Protein Science., 26, 737-748
  8. Kuribayashi, A., Fujii, S., Masanari, M., Yamanaka, M., Wakai, S., Sambongi, Y. (2017) Difference in NaCl tolerance of membrane-bound 5’-nucleotidases purified from deep-sea and brackish water Shewanella species. Extremophiles, 21(2), 357-368
  9. Masanari, M., Fujii, S., Kawahara, K., Oki, H., Maruno, T., Kobayashi, Y., Ohkubo, T., Nishiyama, M., Harada, Y., Wakai, S., Sambongi, Y. (2016) Comparative study on stabilization mechanism of monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea., Biosci Biotechnol Biochem, 80, 2365-2370
    (論文中の結晶構造の図が12月号の表紙に採用)
  10. Wakai, S. (2016) Novel biocides for microbiologically influenced corrosion., EUROCORR proceedings, 55672, 1-11
  11. Ishii, J., Okazaki, F., Djohan, C. Apridah., Hara, Y. K., Asai-Nakashima, N., Teramura, H., Andriani, A., Tominaga, M., Wakai, S., Kahar, P., Yopi, Prasetya, B., Ogino, C., Kondo, A. (2016) From mannan to bioethanol: cell surface co-display of β-mannanase and βmannosidase on yeast Saccharomyces cerevisiae., Biotechnology for Biofuels, 9, 188
  12. Wakai, S., Fujii, S., Masanari, M., Miyanaga, K., Tanji, Y., Sambongi, Y. (2015) Corrosion Test Using Bottom Water from Oil-storage Tank and Microbial Community Analysis by Next Generation Sequencer., Zairyo-to-Kankyo, 64: 540-544
  13. Wakai, S. (2015) Thermodynamic analysis for catalytic reaction under extremely high salt concentration., Journal of Japanese Society for Extremophiles, 14(1):14-20
  14. Kamimura, K., Nagata, N., Kikumoto, M., Sharmin, S., Wakai, S., Kanao, T. (2015) Characterization of a putative chromosome segregation and condensation protein (ScpB) in an acidophilic iron-oxidizing bacterium Acidithiobacillus ferrooxidans. Sci. Rep. Fac. Agr. Okayama Univ., 104:5-12
  15. Yamada, R., Yoshie, T., Sakai, S., Wakai, S., Asai-Nakashima, N., Okazaki, F., Ogino, C., Hisada, H., Tsutsumi, H., Hata, Y., Kondo, A. (2015) Effective saccharinefication of kraft pulp by using a cecllulase cocktail prepared from genetically engineered Aspergillus oryzae., Bioscience, Biotechnology, and Biochemistry, 79(6), 1034-1037
  16. Iino, T., Ito, K., Wakai, S., Tsurumaru, H., Ohkuma, M., Harayama, S. (2015) Iron corrosion induced by non-hydrogenotrophic nitrate-reducing Prolixibacter sp. MIC1-1., Appl. Environ. Microb., 81:1839-1846
  17. Wakai, S., Harayama, S. (2015) Isolation of bacteria rapidly adhering on the surface of metallic iron., Materals Technology, 30(B1):38-43
  18. Hara, K.Y., Araki, M., Okai, N., Wakai, S., Hasunuma, T., Kondo A. (Review) (2014) Development of bio-based fine chemical production through synthetic bioengineering, Microbial Cell Factories, 13(1): 173
  19. Wakai, S., Yoshie, T., Asai-Nakashima, N., Yamada, R., Ogino, C., Tsutsumi, H., Hata, Y., Kondo, A. (2014) L-lactic acid production from starch by simultaneous saccharification and fermentation in a genetically engineered Aspergillus oryzae pure culture., Bioresource Technology, 173, 376-383
  20. Wakai, S., Ito, K., Iino, T., Tomoe, Y., Mori, K., Harayama, S. (2014) Corrosion of iron by iodide-oxidizing bacteria isolated from brine in an iodine production facility. Microbial Ecology, 68:519-527
  21. Sasaki, K., Tsuge, Y., Sasaki, D., Teramura, H.,Wakai, S., Kawaguchi, H., Suzuka, T., Ogino, C., Kondo, A. (2014) Increased ethanol production from sweet sorghum juice concentrated by a membrane separation process., Bioresource Technology, 169, 821-825
  22. Fujii, S., Masanari, M., Yamanaka, M., Wakai, S., Yoshihiro Sambongi Y. (2014) High stability of apo-cytochrome c' from thermophilic Hydrogenophilus thermoluteolus. Biosci. Biotechnol. Biochem., 78:1191-1194
  23. Yamada, R., Yoshie, T., Wakai, S., Asai-Nakashima, N., Okazaki, F., Ogino, C., Hisada, H., Tsutsumi, H., Hata, Y., Kondo, A. (2014) Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose., Microbial Cell Factories, 13:71
  24. Masanari, M., Wakai, S., Ishida, M., Kato, C., and Sambongi, Y. (2014) Relationship between growth pressure of four Shewanella species and stability of their cytochromes c5. Extremophiles, 18:617-627
  25. Wakai, S., Fujii, S., Masanari, M., Abe, A., Sambongi. Y. (2013) Corrosion test using bottom water from oil-storage tank and change of microbial community., Zairyo-to-Kankyo, 62, 389-392
  26. Wakai, S., Kidokoro, S., Masaki, K., Nakasone, K., Sambongi, Y. (2013) Constant enthalpy change value during pyrophosphate hydrolysis within the physiological limits of NaCl. J. Biol. Chem., 288, 29247-29251
  27. Fujii, S., Masanari, M., Inoue, H., Yamanaka, M., Wakai, S., Nishihara, H., Sambongi, Y. (2013) High thermal stability and unique trimer formation of cytochrome c’ from thermophilic Hydrogenophilus thermoluteolus., Bioscience, Biotechnology, and Biochemistry, 77, 1677-1681
  28. Wakai, S., Masanari, M., Ikeda, T., Yamaguchi, N., Ueshima, S., Nishihara, H., Sambongi, Y. (2013) Oxidative phosphorylation in a thermophilic, facultative chemoautotroph, Hydrogenophilus thermoluteolus, living prevalently in geothermal niches., Environmental microbiology reports, 5, 235-242
  29. Ito, K., Wakai, S., Tsurumaru, H., Iino, T., Mori, K., Uchiyama, T., Miki, O., Harayama, S. (2011) Iron corrosion by methane producing archaea (MPA) and sulfate reducing bacteria (SRB) utilizing metallic iron as an electron donor., Zairyo-to-Kankyo, 60, 402-410
  30. Masanari, M., Wakai, S., Tamegai, H., Kurihara, T., Kato, C., Sambongi, Y. (2011) Thermal stability of pressure-sensitive Shewanella livingstonensis cytochrome c5., Bioscience, Biotechnology, and Biochemistry, 75,1859-1861
  31. Inoue, H., Wakai, S., Nishihara, H., Sambongi, Y. (2011) Heterologous synthesis of cytochrome c’ by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery.,  FEBS Journal, 278, 2341-2348
  32. Ito, K., Uchiyama, T., Iino, T., Mori, K., Wakai, S., Tsurumaru, H., Miki, O., Harayama, S. (2010) Iron Corrosion under the Enrichment Culture of Anaerobic Microorganisms Utilizing Metallic Iron as an Electron Donor.,  Zairyo-to-kankyo, 59, 298-304
  33. Takenaka, S., Wakai, S., Tamegai, H., Uchiyama, S., Sambongi, Y. (2010) Comparative analysis of highly homologous Shewanella cytochrome c5 for their stability and function.,  Bioscience, Biotechnology, and Biochemistry, 74, 1079-1083
  34. Sano, R., Kameya, M., Wakai, S., Arai, H., Igarashi, Y., Ishii, M., Sambongi, Y.  (2010) Thiosulfate oxidation by a thermo-neutrophilic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus.,  Bioscience, Biotechnology, and Biochemistry, 74, 892-894
  35. Kamimura, K., Okabayashi, A., Kikumoto, M., Manchur, M.A., Wakai, S., Kanao, T. (2010) Analysis of iron- and sulfur-oxidizing bacteria in a treatment plant of acid rock drainage from a Japanese pyrite mine by use of ribulose-1, 5-bisphosphate carboxylase/oxygenase large-subunit gene., Journal of Bioscience and Bioengineering, 109, 244-248
  36. Wakai, S., Sambongi, Y. (2009) Microbial inorganic sulfur oxidation: Present and future.,  Journal of Japanese Society for Extremophiles, 8, Special Issue, 39-43
  37. Wakai, S., Ito, K., Iino, T., Tomoe, Y., Harayama, S. (2008) Microbiologically influenced corrosion by iodide-oxidizing bacteria.,  International Corrosion Congress proceedings, 3738, 1-9
  38. Ito, K., Wakai, S., Tsurumaru, H., Iino, T., Mori, K., Uchiyama, T., Miki, O., Harayama, S. (2008) Cooperative effect of Methane Producing Archaea (MPA) and Sulfate Reducing Bacteria (SRB) on Corrosion of Iron coated with Tar Epoxy Resin.,  International Corrosion Congress proceedings, 3737, 1-13
  39. Ikeda, S., Tsurumaru, H., Wakai, S., Noritake, C., Fujishiro, K., Akasaka, M., Ando, K. (2008) Evaluation of the Effects of Different Additives in Improving the DNA Extraction Yield and Quality from Andosol.,  Microbes and Environments, 23, 159-166
  40. Ito, K., Wakai, S., Tsurumaru, H., Iino, T., Mori, K., Uchiyama, T., Miki, O., Harayama, S.  (2008) Microbiologically Influenced Corrosion of Iron by Methane Producing Archaea (MPA) associated with Sulfate Reducing Bacteria (SRB).,  EUROCORR proceedings, 1063, 1-11
  41. Ito, K., Uchiyama, T., Mori, K., Iino, T., Tsurumaru, H., Wakai, S., Miki, O., Harayama, S. (2007) Iron Corrosion by Methanogenic Archaea using CO2 as sole carbon source.,  EUROCORR proceedings, 1047, 1-10
  42. Wakai, S., Tsujita, M., Kikumoto, M., Manchur, M.A., Kanao, T., Kamimura, K.  (2007) Purification and characterization of sulfide:quinone oxidoreductase from an acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans.,  Bioscience, Biotechnology, and Biochemistry, 71, 2735-2742
  43. Wakai, S., Yamamoto, K., Kanao, T., Sugio, T., Kamimura, K. (2006) Discrimination among the three Acidithiobacillus species, A. ferrooxidans, A. thiooxidans, and A. caldus, based on restriction fragment length polymorphism analysis of the 16S-23S rDNA intergenic spacer region.,  Sci. Rep. Fac. Agr. Okayama Univ., 95, 7-11
  44. Wakai, S., Ohmori, A., Kanao, T., Sugio, T., Kamimura, K. (2005) Purification and biochemical characterization of the F1-ATPase from Acidithiobacillus ferrooxidans NASF-1 and analysis of the atp operon.,  Bioscience, Biotechnology, and Biochemistry, 69, 1884-1891
  45. Okabayashi, A., Wakai, S., Kanao, T., Sugio, T., Kamimura, K. (2005) Diversity of 16S ribosomal DNA-defined bacterial population in acid rock drainage from Japanese pyrite mine.,  Journal of Bioscience and Bioengineering, 100, 644-652
  46. Wakai, S., Kikumoto, M., Kanao, T., Kamimura, K. (2004) Involvement of sulfide:quinone oxidoreductase in sulfur oxidation of an acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans NASF-1.,  Bioscience, Biotechnology, and Biochemistry, 68, 2519-2528
  47. Kamimura, K., Wakai, S., Sugio, T. (2001) Identification of Thiobacillus ferrooxidans strains based on restriction fragment length polymorphism (RFLP) analysis of 16S rDNA.,  Microbios, 105, 141-152
  48. Kamimura, K., Kunomura, K., Wakai, S., Murakami, K., Sugio, T. (2001) Some properties of a novel obligately autotrophic iron-oxidizing bacterium isolated from seawater.,  Hydrometallurgy, 59, 373-381

総説・解説等

  1. Wakai, S., Sambongi, Y. Extremophilic enzymes related to energy conversion, Role of Water in ATP Hydrolysis Energy Transduction by Protein Machinery, Springer, Makoto Suzuki (Eds.), 275-302 (2018)
  2. 若井暁,加熱で活性型になるバイオサイド,BIO INDUSTRY,vol. 35(4),26-35 (2018)
  3. 若井暁, 微生物腐食を防ぐ新薬剤, 配管技術, vol.60(2), 22-28 (2018)
  4. 若井暁,森浩二,鉄と微生物で水素をつくりだす!―燃料用水素の大量生産技術の実現に向けて,化学,71(9),29-33 (2016)
  5. Wakai, S., Gene Analysis for the Evaluation of the Effect of Environmental Factors. Corrosion Control and Surface Control: Environmentally Friendly Approaches, Springer Japan, Kanematsu, Hideyuki, Barry, Dana M. (Eds.), 169-184 (2016)
  6. 若井暁, 金属材料が患う微生物感染症―微生物腐食―, 化学と生物, vol.53(8), 515-520 (2015)
  7. 荻野千秋,中村聡子,岡井直子,若井暁,川口秀夫,近藤昭彦,バイオマスからの微生物によるバイオモノマー生産,ケミカルエンジニヤリング,Vol.59, No.7, 22-31(2014)
  8. 若井暁, 政成美沙, 渡邉朋子, 三本木至宏, 石油貯蔵タンクの微生物群集解析, 検査技術, Vol.17 No.6, 1-5 (2012)
  9. 若井暁, 味気のないエネルギーの微生物によるおいしい話, 日本生物工学会誌, 5月号, 251 (2012)
  10. 若井暁, 変わりモノの微生物学, 日本生物工学会誌, 7月号, 368 (2010)
  11. 若井暁, 三本木至宏, 微生物のイオウ代謝機能の産業応用-Industrial application of microbial sulfur metabolism-, バイオサイエンスとインダストリー, vol. 68 No. 5, 327-331 (2010)
  12. 若井暁, 原山重明, 金属腐食性微生物による腐食における金属付着性細菌の影響, 第60回日本生物工学会大会トピックスガイド, 19-20 (2008)