メンバー紹介

特命助教裏地 美杉 Misugi Uraji

経歴

1997/03
広島大学 理学部 生物科学科 卒業
1999/03
広島大学 理学研究科 生物科学専攻博士課程前期 修了
2002/03
広島大学 理学研究科 生物科学専攻博士課程後期 修了
2002/04
岡山県生物科学総合研究所 微生物工学部門 流動研究員
2006/03
岡山大学 農学部 生物情報化学研究室 非常勤研究員
2012/04
岡山県農林水産総合センター 生物科学研究所 酵素機能化学 流動研究員
2016/04
岡山県農林水産総合センター 生物科学研究所 酵素機能化学 特別流動研究員
2017/04
関西福祉大学 非常勤講師 兼任(生化学 ~2017年9月まで)
2018/04
神戸大学大学院 科学技術イノベーション研究科 特命助教

学会活動

2010
日本植物生理学会
2013
日本農芸化学会
2013
日本生物工学会

受賞等

2017/05
公益社団法人 山陽技術振興会 第12回村川・難波技術奨励賞

発表論文

  1. Uraji, M., Tamura, H., Mizohata, E., Arima, J., Wan, K., Ogawa, K., Inoue, T., Hatanaka, T. Loop of Streptomyces feruloyl esterase plays an important role in its activity of releasing ferulic acid from biomass, Applied and Environmental Microbiology, in press
  2. Kawakami, K., Moritani, C., Uraji, M., Fujita, A., Kawakami, K., Hatanaka, T., Suzaki, E., Tsuboi, S. (2017) Hepatoprotective effects of rice-derived peptides against acetaminophen-induced damage in mice. Journal of Clinical Biochemistry and Nutrition, 60(2): 115-120
  3. Hoque, TS., Uraji, M., Hoque, MA., Nakamura, Y., Murata, Y. (2017) Methylglyoxal induces inhibition of growth, accumulation of anthocyanin, and activation of glyoxalase I and II in Arabidopsis thaliana, Journal of Biochemical and Molecular Toxicology, 31(7), e21901
  4. Yokotani, N., Uraji, M., Hara, M., Hihara, S., Hatanaka, T., Oda, K. (2016) Low accumulation of chlorogenic acids represses reddening during flesh browning in Japanese peach "Okayama PEH7", Bioscience, Biotechnology, and Biochemistry, 23: 1-6
  5. Kumagai Y, Uraji, M., Wan K, Okuyama M, Kimura A, Hatanaka T. (2016) Molecular insights into the mechanism of thermal stability of actinomycete mannanase, FEBS Letters, 590(17): 2862-2869
  6. Hatanaka, T., Uraji, M., Fujita, A., Kawakami, K. (2015) Anti-oxidation Activities of Rice-Derived Peptides and Their Inhibitory Effects on Dipeptidylpeptidase-IV, Int J Pep Res Ther, 21(4): 479-485
  7. Kumagai, Y., Yamashita, K., Tagami, T., Uraji, M., Wan, K., Okuyama, M., Yao, M., Kimura, A., Hatanaka, T. (2015) The loop structure of Actinomycete glycoside hydrolase family 5 mannanases governs substrate recognition, FEBS J, 282(20): 4001-4014
  8. Sobahan, MA., Akter, N., Okuma, E., Uraji, M., Ye, W., Mori, IC., Nakamura, Y., Murata, Y. (2015) Allyl isothiocyanate induces stomatal closure in Vicia faba, Bioscience, Biotechnology, and Biochemistry, 79(10): 1737-1742
  9. Khokon, MA., Salam, MA., Jammes, F., Ye, W., Hossain, MA., Uraji, M., Nakamura, Y., Mori, IC., Kwak, JM., Murata, Y. (2015) Two guard cell mitogen-activated protein kinases, MPK9 and MPK12, function in methyl jasmonate-induced stomatal closure in Arabidopsis thaliana, Plant Biol (Stuttg), 17(5): 946-952
  10. Kawakami, K., Li, P., Uraji, M., Hatanaka, T., Ito, H. (2014) Inhibitory Effects of pomegranate extracts on recombinant human maltase-glucoamylase, J Food Sci, 79(9): 1848-1853
  11. Uraji, M., Arima, J., Inoue, Y., Harazono, K., Hatanaka, T. (2014) Application of two newly identified and characterized feruloyl esterases from Streptomyces sp. in the enzymatic production of ferulic acid from agricultural biomass, PLoS One, 9(8): e104584
  12. Hatanaka, T., Kawakami, K., Uraji, M. Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity, Journal of Enzyme Inhibition and Medicinal Chemistry, 29(6): 823-828
  13. Hossain, MS., Ye, W., Hossain, MA., Okuma, E., Uraji, M., Nakamura, Y., Mori, IC., Murata, Y. (2013) Glucosinolate degradation products, isothiocyanates, nitriles, and thiocyanates, induce stomatal closure accompanied by peroxidase-mediated reactive oxygen species production in Arabidopsis thaliana, Biosci Biotechnol Biochem, 77(5): 977-983
  14. Uraji, M., Kimura, M., Inoue, Y., Kawakami, K., Kumagai, Y., Harazono, K., Hatanaka, T. (2013) Enzymatic production of ferulic acid from defatted rice bran by using a combination of bacterial enzymes, Appl Biochem Biotechnol, 171(5): 977-987
  15. Kumagai, Y., Kawakami, K., Uraji, M., Hatanaka, T. (2013) Binding of bivalent ions to actinomycete mannanase is accompanied by conformational change and is a key factor in its thermal stability, Biochim Biophys Acta, 1834(1): 301-307
  16. Kumagai, Y., Kawakami, K., Uraji, M., Hatanaka, T. (2013) Effect of binding of bivalent ion to the calcium-binding site responsible for the thermal stability of actinomycete mannanase, J Mol Catal B Enzym, 94: 63-68
  17. Akter, N., Sobahan, MA., Uraji, M., Ye, W., Hossain, MA., Mori, IC., Nakamura, Y., Murata, Y. (2012) Effects of depletion of glutathione on abscisic acid- and methyl jasmonate-induced stomatal closure in Arabidopsis thaliana, Biosci Biotechnol Biochem, 76(11): 2032-2037
  18. Hoque, TS., Uraji, M., Wenxiu, Ye., Hossain, MA., Nakamura, Y., Murata, Y. (2012) Metylglyoxal-induced stomatal closure accpmpanied by peroxidase-mediated ROS production in Arabidopsis, J Plant Physiol, 169(10): 979-986
  19. Hoque, TS., Uraji, M., Tuya, A., Nakamura, Y., Murata, Y. (2012) Metylglyoxal inhibits seed germination and root elongation and uo-regulates transcription of stress-responsive genes in ABA-dependent pathway in Arabidopsis, Plant Biol (Stuttg), doi: 10.1111/j.1438-8677.2012.00607.x
  20. Ohno, M., Uraji, M., Shimoishi, Y., Mori, IC., Nakamura, Y., Murata, Y. (2012) Mechanisms of the selenium tolerance of the Arabidopsis thaliana knockout mutant of sulfate transporter SULTR1;2, Biosci Biotechnol Biochem, 76(5): 993-998
  21. Hoque, MA., Uraji, M., Torii, A., Banu, MN., Mori, IC., Nakamura, Y., Murata, Y. (2012) Methylglyoxal inhibition of cytosolic ascorbate peroxidase from Nicotiana tabacum, J Biochem Mol Toxicol, 26(8): 315-321
  22. Jannat, R., Uraji, M., Hossain, MA., Islam, MM., Nakamura, Y., Mori, IC., Murata, Y. (2012) Catalases negatively regulate methyl jasmonate signaling in guard cells, J Plant Physiol, 169(10): 1012-1016
  23. Uraji, M., Katagiri, T., Okuma, E., Ye, W., Hossain, MA., Masuda, C., Miura, A., Nakamura, Y., Mori, IC., Shinozaki, K., Murata, Y. (2012) Cooperative function of PLDδ and PLDα1 in ABA-induced stomatal closure in Arabidopsis, Plant Physiol, 159(1): 450-460
  24. Sugiyama, Y., Uraji, M., Watanabe-Sugimoto, M., Okuma, E., Munemasa, S., Shimoishi, Y., Nakamura, Y., Mori, IC., Iwai, S., Murata, Y. (2012) FIA functions as an early signal component of abscisic acid signal cascade in Vicia faba guard cells, J Exp Bot, 63(3): 1357-1365
  25. Hoque, TS., Okuma, E., Uraji, M., Furuichi, T., Sasaki, T., Hoque, MA., Nakamura, Y., Mori, IC., Murata, Y. (2012) Inhibitory effects of Methylglyoxal on light-induced stomatal opening and inward K+ channel activity in Arabidopsis, Biosci Biotechnol Biochem, 76(3): 617-619
  26. Jannat, R., Uraji, M., Morofuji, M., Hossain, MA., Islam, MM., Nakamura, Y., Mori, IC., Murata, Y. (2011) The roles of CATALASE2 in abscisic acid signaling in Arabidopsis guard cells, Biosci Biotechnol Biochem, 75(10): 2034-2036
  27. Jannat, R., Uraji, M., Morofuji, M., Islam, MM., Bloom, RE., Nakamura, Y., McClung, CR., Schroeder, JI., Mori, IC., Murata, Y. (2011) Roles of intracellular hydrogen peroxide accumulation in abscisic acid signaling in Arabidopsis guard cells, J Plant Physiol, 168(16): 1919-26
  28. Hossain, MA., Munemasa, S., Uraji, M., Nakamura, Y., Mori, IC., Murata, Y. (2011) Involvement of endogenous abscisic acid in methyl jasmonate-induced stomatal closure in Arabidopsis, Plant Physiol, 156(1): 430-8
  29. Khokon, AR., Okuma, E., Hossain, MA., Munemasa, S., Uraji, M., Nakamura, Y., Mori, IC., Murata, Y. (2011) Involvement of extracellular oxidative burst in salicylic acid-induced stomatal closure in Arabidopsis, Plant Cell Environ, 34(3): 434-43
  30. Khokon, MA., Hossain, MA., Munemasa, S., Uraji, M., Nakamura, Y., Mori, IC., Murata, Y. (2010) Yeast elicitor-induced stomatal closure and peroxidase-mediated ROS production in Arabidopsis, Plant Cell Physiol, 51(11): 1915-1923
  31. Hoque, MA., Uraji, M., Banu, MNA., Mori, IC., Nakamura, Y., Murata, Y. (2010) Effects of methylglyoxal on glutathione S-transferase from Nicotiana tabacum, Biosci Biotechnol Biochem, 74(10): 2124-6
  32. Banu, MNA., Hoque, MA., Watanabe-Sugimoto, M., Islam, MM., Uraji, M., Matsuoka, K., Nakamura, Y., Murata, Y. (2010) Proline and glycinebetaine ameliorate NaCl stress via scavenging hydrogen peroxide and methylglyoxal but not superoxide and nitric oxide in Tobacco Cultured Cells, Biosci Biotechnol Biochem, 74(10): 2043-9
  33. Khokon, MAR., Uraji, M., Munemasa, S., Okuma, E., Nakamura, Y., Mori, IC., Murata, Y*. (2010) Chitosan-Induced Stomatal Closure Accompanied with Peroxidase-Mediated ROS Production in Arabidopsis, Biosci Biotechnol Biochem, 74(11): 2313-5
  34. Akter, N., Sobahan, MA., Hossain, MA., Uraji, M., Nakamura, Y., Mori, IC., Murata, Y. (2010) The involvement of Intracellular Glutathione in Methyl Jasmonate Signaling in Arabidopsis Guard Cells, Biosci Biotechnol Biochem, 74(12): 2504-6
  35. Islam, MM., Tani, C., Watanabe-Sugimoto, M., Uraji, M., Jahan, MS., Masuda, C., Nakamura, Y., Mori, IC., Murata, Y. (2009) Myrosinases, TGG1 and TGG2, redundantly function in ABA and MeJA signaling in Arabidopsis guard cells, Plant Cell Physiol, 50(6): 1171-5
  36. Hatanaka, T., Onaka, H., Arima, J., Uraji, M., Uesugi, Y., Usuki, H., Nishimoto, Y., Iwabuchi, M. (2008) pTONA5: a hyperexpression vector in Streptomycetes, Protein Expr Purif, 62(2): 244-8
  37. Uraji, M., Arima, J., Uesugi, Y., Iwabuchi, M., Hatanaka, T. (2007) Effect of salt on the activity of Streptomyces prolyl aminopeptidase, Biochim Biophys Acta, 1774(11): 1462-9
  38. Hatanaka, T., Arima, J., Uraji, M., Uesugi, Y., Iwabuchi, M. (2007) Characterization, cloning, sequencing and expression of a novel aminopeptidase N from Streptomyces sp. TH-4, Appl Microbiol Biotechnol, 74(2): 347-56
  39. Yamamoto, S., Uraji, M., Tanaka, K., Moriguchi, K., Suzuki, K. (2007) Identification of pTi-SAKURA DNA region conferring enhancement pf plasmid incompatibility and stability, Genes Genet Syst, 82(3): 197-206
  40. Arima J, Uesugi Y, Uraji, M., Iwabuchi M, Hatanaka T. (2006) Dipeptide synthesis by leucine aminopeptidase from Streptomyces septatus TH-2 and its application for synthesis of biologically active peptides, Appl Environ Microbiol, 72(6): 4225-31
  41. Arima, J., Uesugi, Y., Uraji, M., Iwabuchi, M., Hatanaka, T. (2006) The role of Glu196 in the environment around the substrate binding site of leucine aminopeptidase from Streptomyces griseus, FEBS Letter, 580(3): 912-7
  42. Arima, J., Uesugi, Y., Uraji, M., Yatsushiro, S., Tsuboi, S., Iwabuchi, M., Hatanaka, T. (2006) Modulation of Streptomyces leucine aminopeptidase by calcium: identification and functional analysis of key residues in activation and stabilization by calcium, J Biol Chem, 281(9): 5885-94
  43. Suzuki, K., Uraji, M., De, costa D., Hattori, Y., Ohta, N., Iwata, K., Satou, M., Sonoda, H., Miyahara, K., Masuda, A., Nagata, Y., Urbanczyk, H., Bautista-Zapanta, J., Yoshida, K. (2004) A overview of the Agrobacterial genome, Endocytobiosis Cell Res, 15: 143-150
  44. Yoshida, K., Uraji, M., Hattori, Y., Moriguchi, K., Suzuki, K., Tanaka, N., Kato, A. (2004) Genome structure and evolution of giant plant pathogenic plasmids in Agrobacterium tumefaciens and Agrobacterium rhizogenes, Endocytobiosis Cell Re, 15: 371-378
  45. Uraji, M., Suzuki, K., Yoshida, K. (2002) A novel plasmid curing method using incompatibility of plant pathogenic Ti plasmid in Agrobacterium tumefaciens, Genes Genet Syst, 77(1): 1-9
  46. Hattori, Y., Iwata, K., Suzuki, K., Uraji, M., Ohta, N., Katoh, Yoshida, K. (2001) Sequence characterization of the vir region of a nopaline type Ti plasmid, pTi-SAKURA, Genes Genet Syst, 76(2): 121-30
  47. Hattori, Y., Uraji, M., Suzuki, K., Ohta, N., Iwata, K., Kato, A., Yoshida, K. (2000) Gene list on a plant tumor-inducing plasmid, pTi-SAKURA in Agrobacterium tumefaciens MAFF301001, DNA Res, 31;7(6): 371-80
  48. Suzuki, K., Hattori, Y., Uraji, M., Ohta, N., Katoh, A., Yoshida, K. (2000) Complete nucleotide sequence of a plant tumor-inducing Ti plasmid, Gene, 242(1-2): 331-6
  49. Suzuki, K., Ohta, N., Hattori, Y., Uraji, M., Kato, A., Yoshida, K. (1998) Novel structural difference between nopaline- and octopine-type trbJ genes: construction of genetic and physical map and sequencing of trb/traI and rep gene clusters of a new Ti plasmid pTi-SAKURA, Biochim Biophys Acta, 1396(1): 1-7

著書

  1. Mori, IC., Murata, Y., Uraji, M. Integration of ROS and hormone signaling, Reactive Oxygen Species in Plant Signaling, 25-42 (2009)

特許

  1. 岡山県農林水産総合センター生物科学研究所 畑中唯史, 裏地美杉, 原園幸一, "酵素製剤およびそれを用いたフェルラ酸の製造方法", 2012-203420 (2012)