メンバー紹介

特命准教授柘植 謙爾 Kenji Tsuge

経歴

1993/03
埼玉大学 工学部 環境化学工学科 卒業
1995/03
東京工業大学大学院 総合理工学研究科 環境物理工学専攻 修了
1998/03
東京工業大学大学院 総合理工学研究科 化学環境工学専攻 博士課程修了 博士(工学)
1998/04
日本学術振興会 特別研究員
1999/04
三菱化学生命科学研究所 特別研究員
2006/04
慶応義塾大学先端生命科学研究所 特任講師
2016/04
神戸大学大学院 科学技術イノベーション研究科 特命准教授

発表論文

  1. Tsuge, K., Ano, T., Shoda, M. Characterization of Bacillus subtilis YB8, Coproducer of lipopeptides surfactin and plipastatin B1. The Journal of General and Applied Microbiology, 41, 541-545 (1995)
  2. Tsuge, K., Ano, T., Shoda, M. Isolation of a gene essential for biosynthesis of the lipopeptide antibiotics plipastatin B1 and surfactin in Bacillus subtilis YB8. Archives of Microbiology, 165, 243-251 (1996)
  3. Tsuge, K., Ano, T., Hirai, M., Nakamura, Y., Shoda, M. The genes degQ, pps, and lpa-8 (sfp) are responsible for conversion of Bacillus subtilis 168 to plipastatin production. Antimicrobial Agents and Chemotherapy, 43, 2183-2192 (1999)
  4. Itaya, M., Nagata, T., Shiroishi, T., Fujita, K., Tsuge, K. Efficient cloning and engineering of giant DNAs in a novel Bacillus subtilis genome vector. The Journal of Biochemistry (Tokyo), 128, 869-875 (2000)
  5. Tsuge, K., Ohata, Y., Shoda, M. Gene yerP, involved in surfactin self-resistance in Bacillus subtilis. Antimicrobial Agents and Chemotherapy, 45, 3566-3573 (2001)
  6. Tsuge, K., Akiyama, T., Shoda, M. Cloning, sequencing, and characterization of the iturin A operon. Journal of Bacteriology, 183, 6265-6273 (2001)
  7. Tsuge, K., Itaya, M. Recombinational transfer of 100-kilobase genomic DNA to plasmid in Bacillus subtilis 168. Journal of Bacteriology, 183, 5453-5458 (2001)
  8. Ohashi, Y., Ohshima, H., Tsuge, K., Itaya, M. Far different levels of gene expression provided by an oriented cloning system in Bacillus subtilis and Escherichia coli. FEMS Microbiol Letters, 221, 125-130 (2003)
  9. Kaneko, S., Tsuge, K., Takeuchi, T., Itaya, M. Conversion of sub-megasized DNA to desired structures using a novel Bacillus subtilis genome vector. Nucleic Acids Research, 31, e112 (2003)
  10. Tsuge, K., Matsui, K., Itaya, M. One step assembly of multiple DNA fragments with a designed order and orientation in Bacillus subtilis plasmid Nucleic Acids Research, 31, e133 (2003)
  11. Itaya, M., Fujita, K., Ikeuchi, M., Koizumi, M., Tsuge, K. Stable positional cloning of long continuous DNA in the Bacillus subtilis genome vector. The Journal of Biochemistry (Tokyo), 134, 513-519 (2003)
  12. Tomita, S., Tsuge, K., Kikuchi, Y., Itaya, M. Targeted isolation of a designated region of the Bacillus subtilis genome by recombinational transfer. Applied and Environmental Microbiology, 70, 2508-2513 (2004)
  13. Tomita, S., Tsuge, K., Kikuchi, Y., Itaya, M. Region dependent efficiency for recombinational transfer of the Bacillus subtilis 168 genome. Bioscience, Biotechnology, and Biochemistry, 68, 1382-1384 (2004)
  14. Uotsu-Tomita, R., Kaneko, S., Tsuge, K., Itaya, M. Insertion of unmarked DNA sequences in multiple loci of the Bacillus subtilis 168 genome: an efficient selection method. Bioscience, Biotechnology, and Biochemistry, 69, 1036-1039 (2005)
  15. Kaneko, S., Akioka, M., Tsuge, K., Itaya, M. DNA shuttling between plasmid vectors and a genome vector: systematic conversion and preservation of DNA libraries using the Bacillus subtilis genome (BGM) vector. Journal of Molecular Biology, 349, 1036-1044 (2005)
  16. Tsuge, K., Inoue, S., Ano, T., Itaya, M., Shoda, M. Horizontal transfer of iturin A operon, itu, to Bacillus subtilis 168 and conversion into an iturin A producer. Antimicrobial Agents Chemotherapy, 49, 4641- 4648 (2005)
  17. Itaya, M., Tsuge, K., Koizumi, M., Fujita, K. Combining two genomes in one Cell: Stable cloning of the cyanobacterium PCC6803 genome in the Bacillus subtilis 168 genome. Proceeding of the National. Academy of Science, USA., 102, 15971-15976 (2005)
  18. Nishizaki, T., Tsuge, K., Itaya, M., Doi, N., Yanagawa, H. Metabolic engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis. Applied and Environmental Microbiology, 73, 1355-1361 (2007)
  19. Tsuge, K., Matsui, K., Itaya, M. Production of the non-ribosomal peptide plipastatin in Bacillus subtilis regulated by three relevant gene blocks assembled in a single movable DNA segment. Journal of Biotechnology, 129, 592-603 (2007)
  20. Kuroki, A., Ohtani, N., Tsuge, K., Tomita, M., Itaya, M. Conjugational transfer system to shuttle giant DNA cloned by Bacillus subtilis genome (BGM) vector. Gene, 399, 72-80 (2007)
  21. Itaya, M., Fujita, K., Kuroki, A., Tsuge, K. Bottom-up genome assembly using the Bacillus subtilis genome vector. Nature Methods, 5, 41-43 (2008)
  22. Nakagawa, Y., Yugi, K, Tsuge, K., Itaya, M., Yanagawa, H., Sakakibara, Y. Operon structure optimization by random self-assembly. Chemistry of Natural Compounds, DOI 10.1007/s11047-009-9141-0 (2009)
  23. Hiroe, A., Tsuge, K., Nomura, C. T., Itaya, M., Tsuge, T. Rearrangement of gene order in the phaCAB operon leads to effective production of ultra-high-molecular-weight poly[(R)-3-hydroxybutyrate] in genetically engineered Escherichia coli. Applied and Environmental Microbiology, 78, 3177-3184 (2012)
  24. Honma, M., Tanaka, K., Konno, K., Tsuge, K., Okuno, T., Hashimotoa, M. Termination of the structural confusion between plipastatin A1 and fengycin IX. Bioorganic & Medichinal Chemistry, 20, 3793-3798 (2012)
  25. Chen, S-K., Chin, W-C., Tsuge, K., Huang, C.C., Li, S-Y. Fermentation approach for enhancing 1-butanol production using engineered butanologenic Escherichia coli. Bioresource Technology, 145, 204-209 (2013)
  26. Tsuge, K., Sato, Y., Kobayashi, Y., Gondo, M., Hasebe, M., Togashi, T., Tomita, M., Itaya, M. Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments. Scientific Reports, 5, 10655 (2015)
  27. Han, C-H., Tsuge, K., Iba, H. Optimization of artificial operon construction by consultation algorithms utilizing LCS. 2016 IEEE Congress on Evolutionary Computation, 4273-4280 (2016)

総説・解説等

  1. 柘植謙爾, 石井純, 荒木通啓, 近藤昭彦, ゲノム合成の潮流のインパクト 微生物による物質生産, 現代化学, 1月号, No.562, <特集>生命をつくる:ゲノム編集の時代, 36-41 (2018)
  2. 柘植謙爾, 板谷光泰, 枯草菌ゲノムベクターを用いた遺伝子集積,Bio ベンチャー,4, 34-37 (2004)
  3. 板谷光泰, 柘植謙爾, 小泉真貴, 藤田京子, ゲノムベクターを用いたゲノム丸ごとクローニング 蛋白質核酸酵素,51, 61-67 (2006)
  4. 柘植謙爾, オペロンデザインへの挑戦,生物工学会誌,85, 415 (2007)
  5. 柘植謙爾, 板谷光泰, ゲノムビルダーおよびゲノムデザイナーとしての枯草菌,日本生物工学会誌 90, 280-283 (2012)
  6. 板谷光泰, 柘植謙爾, 長鎖DNAの合成と合成生物工学での活用,日本生物工学会誌,91, 319-321(2013)
  7. 柘植謙爾, 板谷光泰, 枯草菌を用いた遺伝子集積法のOGAB法による長鎖DNA合成,日本生物工学会誌,93, 527-529 (2015)
  8. 柘植謙爾, 板谷光泰, 第二世代OGAB法が可能にした50個以上のDNA断片集積,バイオサイエンスとインダストリー,73, 471-475 (2015)
  9. 柘植謙爾, 板谷光泰, 合成生物学を意識した,核酸改変技術の現状と展望-5,ゲノム再編集のための遺伝子クラスター単位の構築,化学と生物,印刷中 (2016)

著書

  1. 柘植謙爾, 板谷光泰, 枯草菌による革新的遺伝子集積法と物質生産微生物機能を活用した革新的生産技術の最前線 シー・エム・シー出版社 65-76 (2007)
  2. Tsuge, K., Itaya, M. Chapter 5: Gene Cluster or Operon Design by Ordered Gene Assembly in Bacillus subtilis (OGAB) Method. In Bioengineering: Principles, Methodologies and Applications, Nova Science Publishers, 153-168 (2010)
  3. Itaya, M., Tsuge, K. Construction and manipulation of giant DNA by use of a genome vector. Methods in Enzymol., 498, 427-448 (2011)
  4. 柘植謙爾(他共同執筆者多数), ひらく、ひらく「バイオの世界」14歳からの生物工学入門 日本生物工学会編 (2012)
  5. 柘植謙爾, 板谷光泰, 第1章ゲノムからの視点「ゲノム再構築技術の応用と課題:汎用性,迅速性,コスト」(pp1-9)合成生物工学の隆起―有用物質の新たな生産法構築をめざして― シー・エム・シー出版(2012)
  6. Itaya, M., Tsuge, K. Chapter 9 Genome integrity, instability and construction In Escherichia coli and Bacillus subtilis; the frontiers of molecular microbiology revisited. Sadaie, Y., and Matsumoto, K. eds. Research Signpost (2012)
  7. Itaya, M., Kaneko, S., Tsuge, K. Chapter 4 Efficient and accurate production of de novo designed large-size gene clusters by a novel Bacillus subtilis-based system. In Microbial Production, Anazawa, H., and Shimizu, S. eds. Springer (2014)