メンバー紹介

TOP メンバー(イノベ) 佐々木 建吾

特命准教授佐々木 建吾 Kengo Sasaki

経歴

2000/03
早稲田大学 理工学部 物理学科卒業
2002/03
筑波大学 大学院生命環境科学研究科応用生命共存専攻 修士課程修了
2006/03
東京大学 大学院農学生命科学研究科応用生命工学専攻 博士課程修了 博士(農学)
2006/04
東京大学大学院農学生命科学研究科応用生命工学専攻特定研究員
2007/04
財団法人電力中央研究所バイオテクノロジー領域特別契約研究員
2010/04
東京大学大学院農学生命科学研究科応用生命工学専攻特任研究員
2012/07
神戸大学自然科学系先端融合研究環重点研究部特命助教
2016/04
神戸大学大学院 科学技術イノベーション研究科 特命准教授

発表論文

  1. Zhang, Y., Nakagawa, K., Shibuya, M., Sasaki, K., Takahashi, T., Shintani, T., Yoshida, T., Kamio, E., Kondo, A., Matsuyama, H. (2018) Improved permselectivity of forward osmosis membranes for efficient concentration of pretreated rice straw and bioethanol production, Journal of Membrane Science, 566, 15-24
  2. Wijaya, H., Sasaki, K., Kahar, P., Yopi, Kawaguchi, H., Sazuka, T., Ogino, C., Prasetya, B., Kondo, A. (2018) Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain, Bioresource Technology, 265, 542-547
  3. Nihei, N., Okamoto, H., Furune, T., Ikuta, N., Sasaki, K., Rimbach, G., Yoshikawa, Y., Terao, K. (2018) Dietary α-cyclodextrin modifies gut microbiota and reduces fat accumulation in high-fat-diet-fed obese mice, BioFactors, 44(4), 336-347
  4. Sasaki, K., Sasaki, D., Tsuge, Y., Morita, M., Kondo, A. (2018) Changes in the microbial consortium during dark hydrogen fermentation in a bioelectrochemical system increases methane production during a two-stage process, Biotechnology for Biofuels, 11: 173
  5. Aikawa, S., Inokuma, K., Wakai, S., Sasaki, K., Ogino, C., Chang, JS, Hasunuma, T., Kondo, A. (2018) Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition, Biotechnology for Biofuels, 11: 50
  6. Sasaki, K., Sasaki, D., Kamiya, K., Nakanishi, S., Kondo, A., Kato, S. (2018) Electrochemical biotechnologies minimizing the required electrode assemblies, Current Opinion in Biotechnology, 50, 182-188
  7. Teramura, H., Sasaki, K., Oshima, T., Kawaguchi, H., Ogino, C., Sazuka, T., Kondo, A. (2018) Effective usage of sorghum bagasse: optimization of organosolv pretreatment using 25% 1-butanol and subsequent nanofiltration membrane separation, Bioresource Technology, 252, 157-164
  8. Sasaki, D., Sasaki, K., Ikuta, N., Yasuda, T., Fukuda, I., Kondo, A., Osawa, R. (2018) Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure, Scientific Reports, 11(8), 435
  9. Sasaki, K., Tsuge, Y., Kawaguchi, H., Yasukawa, M., Sasaki, D., Sazuka, T., Kamio, E., Ogino, C., Matsuyama, H., Kondo, A. (2017) Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process, Applied Microbiology and Biotechnology, 101(15): 6007-6014
  10. Sasaki, K., Sasaki, D., Okai, N., Tanaka, K., Nomoto, R., Fukuda, I., Yoshida, K., Kondo, A., Osawa, R. (2017) Taurine does not affect the composition, diversity, or metabolism of human colonic microbiota simulated in a single-batch fermentation system, PLOS ONE, 12(7), e0180991
  11. Teramura, H., Sasaki, K., Kawaguchi, H., Matsuda, F., Kikuchi, J., Shirai, T., Sazuka, T., Yamasaki, Y., Takumi, S., Ogino, C., Kondo, A. (2017) Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment. Bioscience Biotechnology and Biochemistry, 81(8), 1650-1656
  12. Shibuya, M., Sasaki, K., Tanaka, Y., Yasukawa, M., Takahashi, T., Kondo, A., Matsuyama, H. (2017) Development of combined nanofiltration and forward osmosis process for production of ethanol from pretreated rice straw. Bioresource Technology, 235, 405-410
  13. Shibuya, M., Yasukawa, M., Sasaki, K., Tanaka, Y., Takahashi, T., Kondo, A., Matsuyama, H. (2017) Up-concentration of sugars in pretreated-rice straw by an osmotic pressure-driven method. Biochemical Engineering Journal, 121, 13-16
  14. Sasaki, D., Sasaki, K.,Tsuge, Y., Kondo, A. (2016) Comparative metabolic state of microflora on the surface of the anode electrode in a microbial fuel cell operated at different pH conditions., AMB Express, 6, 125
  15. Takagi, R., Sasaki, K., Sasaki, D., Fukuda, I., Tanaka, K., Yoshida, K., Kondo, A., Osawa, R. (2016) A Single-batch fermentation system to simulate human colonic microbiota for high-throughput evaluation of prebiotics., PLOS ONE,11, e0160533
  16. Sasaki, K., Okamoto, M., Shirai, T., Tsuge, Y., Fujino, A., Sasaki, D., Morita, M., Matsuda, F., Kikuchi, J., Kondo, A. (2016) Toward the complete utilization of rice straw: Methane fermentation and lignin recovery by a combinational process involving mechanical milling, supporting material and nanofiltration ., Bioresource Technology,216, 830-837
  17. Liu, Z., Ho, S.H. Sasaki, K., Haan, R., Inokuma, K., Ogino, C., van Zyl, W.H., Hasunuma, T., Kondo, A. (2016) Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production., Scientific Reports, 6: 24550
  18. Guirimand, G., Sasaki, K., Inokuma, K., Bamba T., Hasunuma, T., Kondo, A. (2016) Cell-surface engineering of Saccharomyces cerevisiae combined with membrane separation technology for xylitol production from rice straw hydrolysate., Applied Microbiology and Biotechnology, 100(8), 3477-3487
  19. Song, J.*, Sasaki, D.*, Sasaki, K., Kato, S., Kondo, A., Hashimoto, K., Nakanishi, N. (*Equally Contribution) Comprehensive metabolomic analyses of anode-respiring Geobacter sulfurreducens cells: the impact of anode-respiration activity on intracellular metabolite levels., Process Biochemistry, 51(1), 34-38
  20. Teramura, H., Sasaki, K., Oshima, T., Matsuda, F., Okamoto, M., Shirai, T., Kawaguchi, H., Ogino, C., Hirano, K., Sazuka, T., Kitano, H., Kikuchi, J., Kondo, A. (2016) Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol., Biotechnology for Biofuels 9: 27
  21. Tsuge, Y., Kawaguchi, H., Sasaki, K., Kondo, A. (Review) (2016)  Engineering cell factories for producing building block chemicals for bio-polymer synthesis., Microbial Cell Factories, 15, 19
  22. Sasaki, K., Hara, K.Y., Kawaguchi, H., Sazuka, T., Ogino, C., Kondo, A. (2016) Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae., Journal of Bioscience and Bioengineering, 121(1), 96-100
  23. Kawaguchi, H., Sasaki, K., Uematsu, K., Tsuge, Y., Teramura, H., Okai, N., Nakamura-Tsuruta, S., Katsuyama, Y., Sugai, Y., Ohnishi, Y., Hirano, K., Sazuka, T., Ogino, C., Kondo, A. (2015) 3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant Corynebacterium glutamicum., Bioresource Technology, 198: 410-417
  24. Sasaki, K., Okamoto, M., Shirai, T., Tsuge, Y., Teramura, H., Sasaki, D., Kawaguchi, H., Hasunuma, T., Ogino, C., Matsuda, F., Kikuchi, J., Kondo, A. (2015) Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content., Biotechnology for Biofuels, 8: 88
  25. Teramura, H., Sasaki, K., Oshima, T., Aikawa, S., Matsuda, F., Okamoto, M., Shirai, T., Kawaguchi, H., Ogino, C., Yamasaki, M., Kikuchi, J., Kondo, A. (2015) Changes in lignin and polysaccharide components in 13 cultivars of rice straw following dilute acid pretreatment as studied by solution-state 2D 1 H-13 C NMR, PLOS ONE, 10(6), e0128417
  26. Sasaki, K., Tsuge, Y., Sasaki, D., Kawaguchi, H., Sazuka, T., Ogino, C., Kondo, A. (2015) Repeated ethanol production from sweet sorghum juice concentrated by membrane separation, Bioresource Technology, 186, 351-355
  27. Sasaki, K., Tsuge, Y., Sasaki, D., Teramura, H., Inokuma,K., Hasunuma, T., Ogino C., Kondo, A. (2015) Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae., Bioresource Technology, 185, 263-268
  28. Sasaki, D., Sasaki, K., Tsuge, Y., Morita, M., Kondo, A. (2014) Comparison of metabolomic profiles of microbial communities between stable and deteriorated methanogenic processes., Bioresource Technology, 172, 83-90
  29. Sasaki, K., Tsuge, Y., Sasaki, D., Teramura, H., Wakai, S., Kawaguchi, H., Suzuka, T., Ogino, C., Kondo, A. (2014) Increased ethanol production from sweet sorghum juice concentrated by a membrane separation process., Bioresource Technology, 169, 821-825
  30. Sasaki, K., Tsuge, Y., Sasaki, D., Hasunuma, T., Sakamoto, T., Sakihama, Y., Ogino, C., Kondo, A. (2014) Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae., Bioresource Technology, 169, 380-386
  31. Tsuge, Y., Kawaguchi, H., Sasaki, K., Tanaka, T., Kondo, A. (2014) Two-step production of D-lactate from mixed sugars by growing and resting cells of metabolically engineered Lactobacillus plantarum., Applied Microbiology and Biotechnology, 98(11), 4911-4918
  32. Joseph, A., Aikawa, S., Sasaki, K., Matsuda, F., Hasunuma, T., Kondo, A. (2014) Increased biomass production and glycogen accumulation in apcE gene deleted Synechocystis sp. PCC 6803., AMB Express, 4:17
  33. Sasaki, K., Tsuge, Y., Sasaki, D., Kondo, A. (2014) Increase in lactate yield by growing Corynebacterium glutamicum in a bioelectrochemical reactor., Journal of Bioscience and Bioengineering, 117(5), 598-601
  34. Joseph, A., Aikawa, S., Sasaki, K., Teramura, H., Hasunuma, T., Matsuda, F., Osanai, T., Hirai-Yokota, M., Kondo, A. (2014) Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803., FEBS Letters, 588(3), 466-471
  35. Tsuge, Y., Tateno, T., Sasaki, K., Hasunuma, T., Tanaka, T., Kondo, A. (2013) Direct production of organic acids from starch by cell surface engineered Corynebacterium glutamicumin anaerobic conditions., AMB Express,3(1):72
  36. Teramura, H., Oshima, T., Matsuda, F,. Sasaki, K.., Ogino, C., Yamasaki, M., Kondo, A. (2013) Glucose content in the liquid hydrolysate after dilute acid pretreatment is affected by the starch content in rice straw., Bioresource Technology, 149, 520-524
  37. Sasaki, K., Sasaki, D., Sakihama, Y., Teramura, H., Yamada, R., Hasunuma, T., Ogino, C., Kondo, A. (2013) Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration., Bioresource Technology, 147, 84-88
  38. Sasaki, K., Morita, M., Sasaki, D., Ohmura, N., Igarashi, Y. (2013) The membraneless bioelectrochemical reactor stimulates hydrogen fermentation by inhibiting methanogenic archaea., Applied Microbiology and Biotechnology, 97(15), 7005-7013
  39. Sasaki, D., Sasaki, K., Watanabe, A., Morita, M., Igarashi, Y., Ohmura, N. (2013) Efficient production of methane from artificial garbage waste by a cylindrical bioelectrochemical reactor containing carbon fiber textiles, Applied Microbiology and Biotechnology Express, 13;3(1), 17
  40. Sasaki, D., Morita, M., Sasaki, K., Watanabe, A., Ohmura, N. (2013) Increased growth of a hydrogenotrophic methanogen in a co-culture with a cellulolytic bacterium under cathodic electrochemical regulation, Bioscience, Biotechnology, and Biochemistry, 77, 1096-1099
  41. Joseph, A., Aikawa, S., Sasaki, K., Tsuge, Y., Matsuda, F., Tanaka, T., Kondo, A. (2013) Utilization of lactic acid bacterial genes in Synechocystis sp. PCC 6803 for the production of lactic acid., Bioscience, Biotechnology, and Biochemistry, 77(5), 966-970
  42. Hirano, S., Matsumoto, N., Morita, M., Sasaki, K., Ohmura, N. (2013) Electrochemical control of redox potential affects methanogenesis of the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus, Letters in Applied Microbiology, 56, 315-321
  43. Sasaki, K., Matsuda, F., Hasunuma, T., Ogino, C., Urairi, M., Yoshida, K., Kondo, A. (2013) Ability of a perfluoropolymer membrane to tolerate by-products of ethanol fermentation broth from dilute acid-pretreated rice straw., Biocehmical Engineering Journal, 70, 135-139
  44. Sasaki, D., Sasaki, K., Watanabe, A., Morita, M., Matsumoto, N., Igarashi, Y., Ohmura, N. (2013) Operation of a cylindrical bioelectrochemical reactor containing carbon fiber fabric for efficient methane fermentation from thickened sewage sludge, Bioresource Technology, 129, 366-373
  45. Sasaki, K., Morita, M., Sasaki, D., Matsumoto, N., Ohmura, N., Igarashi, Y. (2012) Single-chamber bioelectrochemical hydrogen fermentation from garbage slurry., Biochemical Engineering Journal, 68, 104-108
  46. Sasaki, D., Morita, M., Sasaki, K., Watanabe, A., Ohmura, N. (2012) Acceleration of cellulose degradation and shift of product via methanogenic co-culture of a cellulolytic bacterium with a hydrogenotrophic methanogen., Journal of Bioscience and Bioengineering, 114(4), 435-439
  47. Sasaki, K., Morita, M., Matsumoto, N., Sasaki, D., Hirano, S., Ohmura, N., Igarashi, Y. (2012) Construction of hydrogen fermentation from garbage slurry using the membrane free bioelectrochemical system., Journal of Bioscience and Bioengineering, 114(1), 64-69
  48. Sasaki, D., Sasaki, K., Morita, M., Hirano, S., Matsumoto, N., Ohmura, N., (2012) Bioelectrochemical regulation accelerates facultatively syntrophic proteolysis., Journal of Bioscience and Bioengineering, 114(1), 59-63
  49. Morita, M*., Sasaki, K.* (*:Equally contribution), (2012) MINI-REVIEW: Factors influencing the degradation of garbage in methanogenic bioreactors and impacts on biogas formation., Applied Microbiology and Biotechnology, 94(3), 575-582
  50. Sasaki, K., Morita, M., Sasaki, D., Nagaoka, J., Matsumoto, N., Ohmura, N., Shinozaki, H. (2011) Syntrophic degradation of proteinaceous materials by the thermophilic strains Coprothermobacter proteolyticus and Methanothermobacter thermautotrophicus., Journal of Bioscience and Bioengineering, 112(5), 469-472
  51. Sasaki, K., Morita, M., Sasaki, D., Hirano, S., Matsumoto, N., Watanabe, A., Ohmura, N., Igarashi, Y. (2011) A bioelectrochemical reactor containing carbon fiber textiles enables efficient methane fermentation from garbage slurry., Bioresource Technology, 102(13), 6837-6842
  52. Sasaki, K., Morita, M., Hirano, S., Ohmura, N., Igarashi, Y. (2011) Decreasing ammonia inhibition in thermophilic methanogenic bioreactors using carbon fiber textiles., Applied Microbiology and Biotechnology, 90(4), 1555-1561
  53. Sasaki, K., Morita, M., Sasaki, D., Hirano, S., Matsumoto, N., Ohmura, N., Igarashi, Y. (2011) Methanogenic communities on the electrodes of bioelectrochemical reactors without membranes., Journal of Bioscience and Bioengineering, 111(1), 47-49
  54. Sasaki, K., Hirano, S., Morita, M., Sasaki, D., Ohmura, N., Igarashi, Y. (2011) Bioelectrochemical system accelerates microbial growth and degradation of filter paper., Applied Microbiology and Biotechnology, 89(2), 449-455
  55. Yoshida, N., Ohmura, N., Matsumoto, N., Sasaki, K., Saiki, H., Arai, H., Ishii, M., Igarashi, Y. (2010) Dissimilatory Fe(III) reduction by cytochrome c-552 in a thermophilic, obligately chemolithoautotrophic bacterium, Hydrogenobacter thermophilus TK-6., Journal of Japanese Society for Extremophiles, 9(2), 61-66
  56. Sasaki, K., Morita, M., Hirano, S., Sasaki, D., Ohmura, N., Igarashi, Y. (2010) Efficient degradation of rice straw in the reactors packed by carbon fiber textiles., Applied Microbiology and Biotechnology, 87(4), 1579-1586
  57. Sasaki, K., Sasaki, D., Morita, M., Hirano, S., Matsumoto, N., Ohmura, N., Igarashi, Y. (2010) Bioelectrochemical system stabilizes methane fermentation from garbage slurry., Bioresource Technology, 101(10), 3415-3422
  58. Sasaki, K., Sasaki, D., Morita, M., Hirano, S., Matsumoto, N., Ohmura, N., Igarashi, Y. (2010) Efficient treatment of garbage slurry in methanogenic bioreactor packed by fibrous sponge with high porosity., Applied Microbiology and Biotechnology, 86(5), 1573-1583
  59. Sasaki, K., Morita, M., Hirano, S., Ohmura, N., Igarashi, Y. (2009) Effect of adding carbon fiber textiles to methanogenic bioreactors used to treat an artificial garbage slurry., Journal of Bioscience and Bioengineering, 108(2), 130-135
  60. Sasaki, K., Haruta, S., Ueno, Y., Ishii, M., Igarashi, Y. (2007) Microbial population in the biomass adhering to supporting material in a packed-bed reactor degrading organic solid waste., Applied Microbiology and Biotechnology, 75(4), 941-952
  61. Sasaki, K., Haruta, S., Ueno, Y., Ishii, M., Igarashi, Y. (2006) Archaeal population on supporting material in a methanogenic packed-bed reactor., Journal of Bioscience and Bioengineering, 102 (3), 244-246
  62. Sasaki, K., Haruta, S., Tatara, M., Yamazawa, A., Ueno, Y., Ishii, M., Igarashi, Y. (2006) Microbial community in methanogenic packed-bed reactor successfully operating at short hydraulic retention time., Journal of Bioscience and Bioengineering, 101 (3), 271-273

総説・解説等

  1. 生田直子, 篠原涼平, 佐々木大介, 佐々木建吾, 培養系ヒト腸内細菌叢モデルKUHIMMを利用した食物繊維の影響評価, New Food Industry, Vol.60, No.5, 37-43 (2018)
  2. 佐々木建吾, In vitro培養系ヒト大腸菌叢モデルを利用したプレバイオティクスおよびタウリンの影響評価, 日本醸造協会誌, 第113巻, 第2号, 71-78 (2018)
  3. 澁谷真史,中川敬三,佐々木建吾,近藤昭彦,松山秀人, FOプロセスの適用によるバイオエタノール生産の向上, 膜,43(1), 21-26 (2018)
  4. 佐々木建吾, 星奈美子, 16S rRNA遺伝子から見る腸内細菌叢の魅力, 生物工学会誌, 第95巻, 第7号, 397-400 (2017)
  5. 佐々木建吾,近藤昭彦,次世代のバイオ水素エネルギー,1章 バクテリアによる水素発酵および電気化学の適用,化学同人,44-50(2014)
  6. 佐々木建吾,佐々木大介,森田仁彦,五十嵐泰夫,近藤昭彦,特集 電気化学システムを用いた模擬生ごみからの水素発酵の制御,一般社団法人 水素エネルギー協会(HESS)会誌,38(1),33-37 (2013)