メンバー紹介

特命助教大西 美輪 Miwa Ohnishi

経歴

1999/03
奈良女子大学 理学部 生物学科 卒業
2001/03
奈良女子大学大学院 人間文化研究科 生物科学専攻 修了
2001/04
東京ビジネスサービス株式会社
奈良女子大学 理学部 生物科学科 研究開発業務
2002/04
奈良女子大学 理学部 非常勤研究員
2003/03
科学技術振興事業団CREST 技術員
2006/04
神戸大学 理学部 学術推進研究員
2007/04
神戸大学大学院 理学研究科 学術推進研究員
2011/10
神戸大学大学院 理学研究科 特命助教
2015/04
神戸大学 自然科学系先端融合研究環 特命助教
2016/04
神戸大学 先端融合研究環 特命助教
2018/04
神戸大学大学院 科学技術イノベーション研究科 特命助教
2018/11
神戸大学 先端バイオ工学研究センター 特命助教
神戸大学大学院 科学技術イノベーション研究科 兼任

発表論文

  1. Ohnishi, M., Anegawa, A., Sugiyama, Y., Harada, K., Oikawa, A., Nakayama, Y., Matsuda, F., Nakamura, Y., Sasaki, R., Shichijo, C., Hatcher, PG., Fukaki, H., Kanaya, S., Aoki, K., Yamazaki, M., Fukusaki, E., Saito, K., Mimura, T. (2018) Molecular components of Arabidopsis intact vacuoles clarified with metabolomic and proteomic analyses. Plant and Cell Physiology, 59(7): 1353-1362
  2. Ohnishi, M., Yoshida, K., Mimura, T. (2018) Analyzing the vacuolar membrane (tonoplast) proteome, Plant Membrane Proteomics. Humana Press, New York, NY, 107-116
  3. Kurita, Y., Baba, KI., Ohnishi, M., Matsubara, R., Kosuge, K., Anegawa, A., Shichijo, C., Ishizaki, K., Kaneko, Y., Hayashi, M., Suzaki, T., Fukaki, H., Mimura, T. (2017) Inositol Hexakis Phosphate is the Seasonal Phosphorus Reservoir in the Deciduous Woody Plant Populus alba L, Plant and Cell Physiology, 58(9): 1477-1485
  4. Hayashi, M., Tanaka, M., Yamamoto, S., Nakagawa, T., Kanai, M., Anegawa, A., Ohnishi, M., Mimura, T., Nishimura, M. (2017) Plastidial Folate Prevents Starch Biosynthesis Triggered by Sugar Influx into Non-photosynthetic Plastids of Arabidopsis, Plant and Cell Physiology, 58(8): 1328-1338
  5. Sharma, SS., Yamamoto, K., Hamaji, K., Ohnishi, M., Anegawa, A., Sharma S., Thakur, S., Kumar, V., Uemura, T., Nakano, A., Mimura, T. (2017) Cadmium-induced changes in vacuolar aspects of Arabidopsis thaliana, Plant Physiology and Biochemistry, 114: 29-37
  6. 高橋勝利, 姉川彩, 大西美輪, 山本浩太郎, 石崎公庸, 深城英弘, 三村徹郎, (2016) 植物組織における低分子量物質分布の質量顕微鏡による可視化, Plant Morphology, 28, 23-27
  7. Chiang, CP., Yim, WC., Sun, YH., Ohnishi, M., Mimura, T., Cushman, JC., Yen, HE. (2016) Identification of Ice Plant (Mesembryanthemum crystallinum L.) MicroRNAs Using RNA-Seq and Their Putative Roles in High Salinity Responses in Seedlings, Frontiers in Plant Science, 7: 1143
  8. Yamamoto, K., Takahashi, K., Mizuno, H., Anegawa, A., Ishizaki, K., Fukaki, H., Ohnishi, M., Yamazaki, M., Masujima, T., Mimura, T. (2016) Cell-specific localization of alkaloids in Catharanthus roseus stem tissue measured with Imaging MS and Single-cell MS, Proc Natl Acad Sci U S A, 113(14): 3891-3896
  9. Ohnishi, M., Kadohama, N., Suzuki, Y., Kajiyama, T., Shichijo, C., Ishizaki, K., Fukaki, H., Iida, H., Kambara, H., Mimura, T. (2015) Involvement of Ca2+ in vacuole degradation caused by a rapid temperature decrease in Saintpaulia palisade cells: A case of gene expression analysis in a specialized small tissue, Plant & Cell Physiology, 56(7): 1297-1305
  10. Okazaki, Y., Nishizawa, T., Takano, K., Ohnishi, M., Mimura, T., Saito, K. (2015) Induced accumulation of glucuronosyldiacylglycerol in tomato and soybean under phosphorus deprivation, Physiologia Plantarum, 155(1): 33-42<
  11. Anegawa, A., Ohnishi, M., Takagi, D., Miyake, C., Shichijo, C., Ishizaki, K., Fukaki, H., Mimura, T. (2015) Altered Levels of Primary Metabolites in Response to Exogenous Indole-3-Acetic Acid in Wild Type and Auxin Signaling Mutants of Arabidopsis thaliana : A Capillary Electrophoresis-Mass Spectrometry Analysis, Plant Biotechnology, 32: 65-79
  12. Kanno, S., Kurita, Y., Ohnishi, M., Mimura, T. (2014) Autoradiography of Pi Distribution in Barley Seedlings, Bio-protocol, 4(13): e1175, http://www.bio-protocol.org/e1175
  13. Kurita, Y., Kanno, S., Ohnishi, M., Mimura, T. (2014) Extraction of Ions from Leaf Sections, Bio-protocol, 4(13): e1174, http://www.bio-protocol.org/e1174
  14. Kurita, Y., Baba, K., Ohnishi, M., Anegawa, A., Shichijo, C., Kosuge, K., Fukaki, H., Mimura, T. (2014) Establishment of a shortened annual cycle system; a tool for the analysis of annual re-translocation of phosphorus in the deciduous woody plant (Populus alba L.), Journal of Plant Research Jul, 127(4): 545-551
  15. Kadohama, N., Goh, T., Ohnishi, M., Fukaki, H., Mimura, T., Suzuki, Y. (2013) Sudden collapse of vacuoles in Saintpaulia sp. palisade cells induced by a rapid temperature decrease, PLoS One, 8(2): e57259
  16. Nagai, M., Ohnishi, M., Uehara, T., Yamagami, M., Miura, E., Kamakura, M., Kitamura, A., Sakaguchi, S., Sakamoto, W., Shimmen, T., Fukaki, H., Reid, RJ., Furukawa, A., Mimura, T. (2013) Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley, Plant Cell & Environment, 36(10): 1826-1837
  17. Yoshida, K., Ohnishi, M., Fukao, Y., Okazaki, Y., Fujiwara, M., Song, C., Nakanishi, Y., Saito, K., Shimmen, T., Suzaki, T., Hayashi, F., Fukaki, H., Maeshima, M., Mimura, T. (2013) Studies on Vacuolar Membrane Microdomains Isolated from Arabidopsis Suspension-Cultured Cells: Local Distribution of Vacuolar Membrane Proteins, Plant & Cell Physiology, 54(10): 1571-1584
  18. Hamaji, K., Nagira, M., Yoshida, K., Ohnishi, M., Oda, Y., Uemura, T., Goh, T., Sato, MH., Morita, MT., Tasaka, M., Hasezawa, S., Nakano, A., Hara-Nishimura, I., Maeshima, M., Fukaki, H., Mimura, T. (2009) Dynamic aspects of ion accumulation by vesicle traffic under salt stress in Arabidopsis, Plant & Cell Physiology, 50(12): 2023-2033
  19. Mimura, T., Ohnishi, M., Shimaoka, T., Tomizawa, K. (2008) Proteome analysis of vacuolar membrane, Plant Genetic Engineering vol 9: Plant membrane and vacuolar transporters, Ed. by Jaiwal PK. pp. 301-343, CABInternatonal
  20. Mitsuhashi, N., Kondo, M., Nakaune, S., Ohnishi, M., Hayashi, M., Hara-Nishimura, I., Richardson, A., Fukaki, H., Nishimura, M., Mimura, T. (2008) Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis, J Exp Bot, 59(11): 3069-3076
  21. 三村徹郎,大西美輪,深城英弘, リン環境と植物, 植物における環境と生物ストレスに対する応答, 蛋白質核酸酵素—別冊52:625-632, 共立出版
  22. Ohnishi, M.*, Mimura, T.*, Tsujimura, T., Mitsuhashi, N., Washitani-Nemoto, S., Maeshima, M., Martinoia E. (* These authors are equally contributed) (2007) Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status, Planta, 225(3):711-718
  23. Mitsuhashi, N., Ohnishi, M., Sekiguchi, Y., Kwon, YU., Chang, YT., Chung, SK., Inoue, Y., Reid, RJ., Yagisawa, H., Mimura, T. (2005) Phytic Acid Synthesis and Vacuolar Accumulation in Suspension-Cultured Cells of Catharanthus roseus Induced by High Concentration of Inorganic Phosphate and Cations, Plant Physiology, 138: 1607-1614
  24. Kobae, Y., Uemura, T., Sato, MH., Ohnishi, M., Mimura, T., Nakagawa, T., Maeshima, M. (2004) Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis, Plant & Cell Physiology, 45: 1749-58
  25. Kataoka, T., Watanabe-Takahashi, A., Hayashi, N., Ohnishi, M., Mimura, T., Buchner, P., Hawkesford MJ., Yamaya, T., Takahashi, H. (2004) Vacuolar Sulfate Transporters Are Essential Determinants Controlling Internal Distribution of Sulfate in Arabidopsis, The Plant Cell, 16: 2693-2704
  26. Shimaoka, T.*, Ohnishi, M.*, Sazuka, T., Mitsuhashi, N., Hara-Nishimura, I., Shimazaki, K., Maeshima, M., Yokota, A., Tomizawa, K., Mimura, T. (* These authors are equally contributed) (2004) Isolation of Intact Vacuoles and Proteomic Analysis of Tonoplast from Suspension-Cultured Cells of Arabidopsis thaliana, Plant & Cell Physiology, 45: 672-683
  27. 三村徹郎,三橋尚登,関口陽子,大西美輪, (2003) リン酸の輸送と細胞内分配 植物の代謝コミュニケーション, 蛋白質核酸酵素—別冊48:2044-2051, 共立出版
  28. Mimura, T, Kura-Hotta, M, Tsujimura, T, Ohnishi, M., Miura, M, Okazaki, Y, Mimura, M, Maeshima, M, Washitani-Nemoto, S. (2003) Rapid increase of vacuolar volume in response to salt stress, Planta, 216(3): 397-402