メンバー紹介

経歴

2009/03

神戸大学　工学部　応用化学科　卒業

2011/03

神戸大学大学院　工学研究科　応用化学専攻　博士前期課程　修了

2011/04

日本学術振興会特別研究員 (DC1)

2014/03

神戸大学大学院　工学研究科　応用化学専攻　博士後期課程　修了　博士(工学)

2014/04

高機能遺伝子デザイン技術研究組合(TRAHED)　研究員

2015/04

神戸大学大学院　工学研究科　学術研究員

2016/04

神戸大学大学院　科学技術イノベーション研究科　学術研究員

2018/04

神戸大学大学院　科学技術イノベーション研究科　特命助教

2018/07

神戸大学　先端バイオ工学研究センター　兼任

受賞等

• 2018. 11.　i-BioN 2018 The Symposium on Biorefinery and Bioprocess Topics, Best Poster Award
• 2013. 12.　神戸大学研究基盤センター「若手フロンティア研究会」, ポスター賞
• 2013. 09.　化学工学会第45回秋季大会バイオ部会, ポスター賞

発表論文

1. Ito, Y., Watanabe, T., Aikawa, S., Nishi, T., Nishiyama, T., Nakamura, Y., Hasunuma, T., Okubo, Y., Ishii, J., Kondo, A. (2018/11) Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii, FEMS Yeast Research, 18(7)
2. Nakamura, Y., Nishi, T., Noguchi, R., Ito, Y., Watanabe, T., Nishiyama, T., Aikawa, S., Hasunuma, T., Ishii, J., Okubo, Y., Kondo, A. (2018) A stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA, Applied and Environmental Microbiology, 84(15), e02882-17
3. Hashi, H., Nakamura, Y., Ishii, J., Kondo, A. (2018) Modifying expression modes of human neurotensin receptor type 1 alters sensing capabilities for agonists in yeast signaling biosensor, Biotechnology Journal, 13(4), e1700522
4. Ryo, S., Ishii, J., Matsuno, T., Nakamura, Y., Matsubara, D., Tominaga, M., Kondo, A. (2017) Positive feedback genetic circuit incorporating a constitutively active mutant Gal3 into yeast GAL induction system., ACS Synthetic Biology, 6(6), 928-935
5. Nakamura, Y., Hashimoto, T., Ishii, J., Kondo, A. (2016) Dual-color reporter switching system to discern dimer formations of G-protein-coupled receptors using Cre/loxP site-specific recombination in yeast., Biotechnology and Bioengineering, 113(10), 2178-2190
6. Nakamura, Y., Ishii, J., Kondo, A.(Review) (2016) Current techniques for studying oligomer formations of G-protein-coupled receptors using mammalian and yeast cells., Current Medicinal Chemistry,23(16)1638-1656
7. Nakamura, Y., Ishii, J., Kondo, A. (2015) Applications of yeast-based signaling sensor for characterization of antagonist and analysis of site-directed mutants of the human serotonin 1A receptor., Biotechnology and Bioengineering, 112(9), 1906-1915
8. Nakamura, Y., Ishii, J., Kondo, A. (2014) Construction of a yeast-based signaling biosensor for human angiotensin II type 1 receptor via functional coupling between Asn295-mutated receptor and Gpa1/Gi3 chimeric Gα., Biotechnology and Bioengineering, 111(11), 2220-2228
9. Nakamura, Y., Ishii, J., Kondo, A. (2014) Signaling assays for detection of human G-protein-coupled receptors in yeast., Bio-protocol, 4(16), e1206
10. Nakamura, Y., Takemoto, N., Ishii, J., Kondo, A. (2014) Simultaneous method for analyzing dimerization and signaling of G-protein-coupled receptor in yeast by dual-color reporter system., Biotechnology and Bioengineering, 111(3), 586-596 [Featured Spotlight Article]
11. Nakamura, Y., Ishii, J., Kondo, A. (2013) Bright fluorescence monitoring system utilizing Zoanthus sp. green fluorescent protein (ZsGreen) for human G-protein-coupled receptor signaling in microbial yeast cells., PLOS ONE, 8(12), e82237
12. Nakamura, Y., Ishii, J., Kondo, A. (2013) Rapid, facile detection of heterodimer partners for target human G-protein-coupled receptors using a modified split-ubiquitin membrane yeast two-hybrid system., PLOS ONE, 8(6), e66793

総説・解説等

1. 中村泰之, 伊藤洋一郎, 梅津光央, 石井純, 近藤昭彦, 低分子抗体医薬の開発展望, バイオインダストリー, Vol.34, No.6, 54-62 (2017)

著書

1. Nakamura, Y., Kondo, A., Ishii, J., Biosensing techniques in yeast: G-protein signaling and protein-protein interaction assays for monitoring ligand stimulation and oligomer formation of heterologous GPCRs, Peripheral Membrane Proteins, ISBN 978-1-78923-513-5, IntechOpen, Chapter 3, 25-48 (2018)