メンバー紹介

特命助教猪熊 健太郎 Kentaro Inokuma

経歴

2003/03
島根大学 生物資源科学部 生命工学科 卒業
2008/03
広島大学大学院 先端物質科学研究科 分子生命機能科学専攻 博士課程後期 修了
2008/04
九州大学大学院 農学研究院 学術研究員
2010/08
富山大学 工学部 研究員
2012/04
神戸大学大学院 工学研究科 学術推進研究員
2013/03
高機能遺伝子デザイン技術研究組合 研究員
2014/04
神戸大学 自然科学系先端融合研究環 重点研究部 学術研究員
2016/04
神戸大学大学院 科学技術イノベーション研究科 特命助教

受賞等

  • 2013/09 平成25年度 日本生物工学会大会 第21回 生物工学論文賞
  • 2011/09 平成23年度 日本生物工学会大会 第19回 生物工学論文賞

発表論文

  1. Bamba, T., Inokuma, K., Hasunuma, T., Kondo, A. (2018) Enhanced cell-surface display of a heterologous protein using SED1 anchoring system in SED1-disrupted Saccharomyces cerevisiae strain., Journal of Bioscience and Bioengineering, 125(3), 306-310
  2. Aikawa, S., Inokuma, K., Wakai, S., Sasaki, K., Ogino, C., Chang, JS, Hasunuma, T., Kondo, A. (2018) Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition, Biotechnology for Biofuels, 11: 50
  3. Inokuma, K., Iwamoto, R., Bamba, T., Hasunuma, T., Kondo, A. (2017) Improvement of xylose fermentation ability under heat and acid co-stress in Saccharomyces cerevisiae using genome shuffling technique, Frontiers in Bioengineering and Biotechnology, 5, 81
  4. Liu, Z., Inokuma, K., Ho, SH., den Haan, R., van Zyl, W.H., Hasunuma, T., Kondo, A. (2017) Improvement of ethanol production from crystalline cellulose via optimizing cellulase ratios in cellulolytic Saccharomyces cerevisiae. Biotechnology and Bioengineering, 114(6), 1201-1207
  5. Takenaka, M., Kobayashi, T., Inokuma, K., Hasunuma, T., Maruyama, T., Ogino, C., Kondo, A. (2017) Mapping of endoglucanases displayed on yeast cell surface using atomic force microscopy., Colloids and Surfaces B: Biointerfaces, 151, 134-142
  6. Inokuma, K., Bamba, T., Ishii, J., Ito, Y., Hasunuma, T., Kondo, A. (2016) Enhanced cell-surface display and secretory production of cellulolytic enzymes with Saccharomyces cerevisiae Sed1 signal peptide., Biotechnology and Bioengineering, 113(11), 2358-2366
  7. Inokuma, K., Hasunuma, T., Kondo, A. (2016) Ethanol production from N-acetyl-D-glucosamine by Scheffersomyces stipitis strains., AMB Express, 6, 83
  8. Liu, Z., Ho, S.H. Sasaki, K., Haan, R., Inokuma, K., Ogino, C., van Zyl, W.H., Hasunuma, T., Kondo, A. (2016) Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production., Scientific Reports, 6: 24550
  9. Guirimand, G., Sasaki, K., Inokuma, K., Bamba T., Hasunuma, T., Kondo, A. (2016) Cell-surface engineering of Saccharomyces cerevisiae combined with membrane separation technology for xylitol production from rice straw hydrolysate., Applied Microbiology and Biotechnology, 100(8), 3477-3487
  10. Liu, Z., Inokuma, K., Ho, S.H., den Haan, R., Hasunuma, T., van Zyl, W.H., Kondo, A. (2015) Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae., Biotechnology for Biofuels, 8: 162
  11. Inokuma, K., Ishii, J., Hara, K.Y., Mochizuki, M., Hasunuma, T., Kondo, A. (2015) Complete genome sequence of Kluyveromyces marxianus NBRC1777, a nonconventional thermotolerant yeast., Genome Announcements , 3(2), e00389-15
  12. Sasaki, K., Tsuge, Y., Sasaki, D., Teramura, H., Inokuma, K., Hasunuma, T., Ogino C., Kondo, A. (2015) Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae., Bioresource Technology, 185, 263-268
  13. Inokuma, K., Yoshida, T., Ishii, J., Hasunuma, T., Kondo, A. (2015) Efficient co-displaying and artificial ratio control of α-amylase and glucoamylase on the yeast cell surface by using combinations of different anchoring domains., Applied Microbiology and Biotechnology, 99(4), 1655-1663
  14. Inokuma, K., Hasunuma T., Kondo A. (2014) Efficient yeast cell-surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter. Biotechnology for biofuels. 7, 8
  15. Inokuma, K., Takano M., Hoshino K. (2013) Direct ethanol production from N-acetylglucosamine and chitin substrates by Mucor species. Biochemical Engineering Journal, 72, 24-32
  16. Soma Y., Inokuma, K., Tanaka T., Ogino C., Kondo A., Okamoto M., Hanai T. (2012) Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system. Journal of Bioscience and Bioengineering, 114 (1), 80-85
  17. Yukihiro Tashiro, Wataru Kaneko, Yanqi Sun, Keisuke Shibata, Inokuma K., Zendo T., Sonomoto K. (2011) Continuous D-lactic acid production by a novel thermotolerant Lactobacillus delbrueckii subsp. lactis QU 41. Applied and Environmental Microbiology, 89 (6), 1741-1750
  18. Inokuma K., Liao J. C., Okamoto M., Hanai T. (2010) Improvement of isopropanol production by metabolically engineered Escherichia coli using gas stripping. Journal of Bioscience and Bioengineering, 110 (6), 696-701
  19. Sakai S., Inokuma K., Nakashimada Y., Nishio N. (2008) Degradation of glyoxylate and glycolate with ATP synthesis by a thermophilic anaerobic bacterium, Moorella sp. strain HUC22-1. Applied and Environmental Microbiology, 74 (5): 1447-1452
  20. Inokuma K., Nakashimada Y., Akahoshi T., Nishio N. (2007) Characterization of enzymes involved in the ethanol production of Moorella sp. HUC22-1. Archives of Microbiology, 188, 37-45
  21. Sakai S., Nakashimada Y., Inokuma K., Kita M., Okada H., Nishio N. (2005) Acetate and ethanol production from H2 and CO2 by Moorella sp. using a repeated batch culture. Journal of Bioscience and Bioengineering, 99, 252-258

総説・解説等

  1. Inokuma, K., Hasunuma, T., Kondo, A. Emerging Areas in Bioengineering:Whole cell biocatalysts using enzymes displayed on yeast cell surface., Chang HN (Ed) Wiley-VCH, 81-92 (2018)
  2. 猪熊健太郎,竹中武蔵,荻野千秋,蓮沼誠久,近藤昭彦,原子間力顕微鏡による酵母細胞表層セルラーゼの局在評価,生物工学,94,698-700(2016)
  3. 猪熊健太郎,蓮沼誠久,近藤昭彦,バイオマスエネルギーの技術と市場 第4章 次世代バイオエタノール製造技術,シーエムシー出版,33-45 (2016)
  4. 猪熊健太郎, 蓮沼誠久, 近藤昭彦, CBPによるバイオエタノール生産技術の開発, バイオインダストリー, Vol.32, No.5, 26-31(2015)
  5. 猪熊健太郎, James C. Liao, 岡本正宏, 花井泰三, ガスストリッピングを用いた組換え大腸菌によるイソプロパノール生産の向上. 生物工学会誌, 90 (2), 78 (2012)
  6. 猪熊健太郎, 花井泰三, 合成代謝経路を導入した大腸菌によるイソプロパノール生産, 化学と生物, 50 (2), 79-80 (2012)