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特命准教授伏見　圭司 Keiji Fushimi

経歴

2007/03
九州東海大学（現：東海大学）　農学部　応用動物科学科 卒業
2009/03
静岡大学　大学院農学研究科　応用生物化学専攻　修士課程　修了
2012/03
静岡大学　創造科学技術大学院　自然科学系教育部　バイオサイエンス専攻　博士後期課程　修了
博士（農学）取得
2012/04
静岡大学　教育学部　協力研究員
2013/04
日本分析化学専門学校　専任講師
2014/04
静岡大学　理学部　学術研究員
2017/04
静岡大学　理学部　特任助教
2018/10
University of California, Davis (John Clark Lagarias 研究室)　客員研究員
2021/01
神戸大学　大学院科学技術イノベーション研究科　特命准教授

受賞等

  • 2021年10月　最優秀ポスター賞, 神戸大学　第１回先端バイオ工学研究センター成果発表会
  • 2011年 6月　第10回新規素材探索研究会奨励賞, 新規素材探索研究会
    フミヅキタケ（Agrocybe praecox）の産生する植物成長調節物質の探索

発表論文

  1. Takemura, H., Choi, JK., Fushimi, K., Narikawa, R., Wu, J., Kondo, M., Nelson, DC., Suzuki, T., Ouchi, H., Inai, M., Hirai, H., Kawagishi, H. (2023) Role of hypoxanthine-guanine phosphoribosyltransferase in the metabolism of fairy chemicals in rice, Org. Biomol. Chem., 21(12), 2556-2561
  2. Kuwasaki, Y., Suzuki, K., Yu, G., Yamamoto, S., Otabe, T., Kakihara, Y., Nishiwaki, M., Miyake, K., Fushimi, K., Bekdash, R., Shimizu,Y., Narikawa, R., Nakajima, T., Yazawa, M., Sato, M. (2022) MagRed: A semi-synthetic photoswitch for red light optogenetics, >Nat. Biotechnol., 40(11), 1672-1679
  3. Y. Makita#, S. Suzuki#, K. Fushimi#, S. Shimada, A. Suehisa, M. Hirata, T. Kuriyama, Y. Kurihara, H. Hamasaki, E. Okubo-Kurihara, K. Yoshitake, T. Watanabe, M. Sakuta, T. Gojobori, T. Sakami, R. Narikawa, H. Yamaguchi, M. Kawachi, M. Matsui, Identification of a dual orange/far-red and blue light photoreceptor from an oceanic green picoplanktonin, Nat. Commun., 21(1), 3593
  4. SR. Tachibana, L. Tang, L. Zhu, Y. Takeda, K. Fushimi, Y. Ueda, T. Nakajima, Y. Kuwasaki, M. Sato, R. Narikawa, C. Fang, (2021/05) An engineered biliverdin-compatible cyanobacteriochrome enables a unique ultrafast reversible photoswitching pathway, Int. J. Mol. Sci., 22(10), 5252
  5. K. Fushimi*, R. Narikawa*, (2021/03) Unusual ring D fixation by three crucial residues promotes phycoviolobilin formation in the DXCF-type cyanobacteriochrome without the second Cys, Biochem. J., 478(5), 1043-1059
  6. SR. Tachibana, L. Tang, C. Chen, L. Zhu, Y. Takeda, K. Fushimi, TK. Seevers, R. Narikawa, M. Sato, C. Fang (2021) Transient electronic and vibrational signatures during reversiblephotoswitching of a cyanobacteriochrome photoreceptor, Spectrochim. Acta A Mol. Biomol. Spectrosc., 250, 119379
  7. K. Fushimi, H. Hoshino, N. Shinozaki-Narikawa, Y. Kuwasaki, K. Miyake, T. Nakajima, M. Sato, F. Kano, R. Narikawa, (2020) The cruciality of single amino acid replacement for the spectral tuning of biliverdin-binding cyanobacteriochromes, Int. J. Mol. Sci., 21(17), 6278
  8. K. Fushimi, T. Matsunaga, R. Narikawa, (2020) A photoproduct of DXCF cyanobacteriochromes without reversible Cys ligation is destabilized by rotating ring twist of the chromophore, Photochem. Photobiol. Sci., 19, 1289-1299
  9. K. Fushimi, M. Hasegawa, T. Ito, NC. Rockwell, G. Enomoto, N-N-Win, JC. Lagarias, M. Ikeuchi, R. Narikawa, (2020) Evolution-inspired design of multicolored photoswitches from a single cyanobacteriochrome scaffold, Proc. Natl. Acad. Sci. USA, 117(27), 15573-15580
  10. K. Miyake, K. Fushimi, T. Kashimoto, K. Maeda, N-N-Win, H. Kimura, M. Sugishima, M. Ikeuchi, R. Narikawa, (2020) Functional diversification of two bilin reductases for light perception and harvesting in unique cyanobacterium Acaryochloris marina MBIC 11017, FEBS Journal, 287(18), 4016-4031
  11. Y. Kuwasaki, K. Miyake, K. Fushimi, Y. Takeda, Y. Ueda, T. Nakajima, M. Ikeuchi, M. Sato, R. Narikawa, (2019) Protein engineering of dual-Cys cyanobacteriochrome AM1_1186g2 for biliverdin incorporation and far-red/blue reversible photoconversion, Int. J. Mol. Sci., 20(12), 2935
  12. K. Fushimi, T. Miyazaki, Y. Kuwasaki, T. Nakajima, T. Yamamoto, K. Suzuki, Y. Ueda, K. Miyake, Y. Takeda, J-H. Choi, H. Kawagishi, EY. Park, M. Ikeuchi, M. Sato, R. Narikawa, (2019) Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin, Proc. Natl. Acad. Sci. USA, 116(17), 8301-8309
  13. 崔 宰熏, 呉 静, 伏見 圭司, 平井 浩文, 河岸 洋和, (2018) シバ Agrostis stolonifera 由来のコムラサキシメジ菌糸体に対する生育抑制物質, 日本きのこ学会誌, 25(4), 141-144
  14. M. Hasegawa, K. Fushimi, K. Miyake, T. Nakajima, Y. Oikawa, G. Enomoto, M. Sato, M. Ikeuchi, R. Narikawa, (2018) Molecular characterization of DXCF cyanobacteriochromes from the cyanobacterium Acaryochloris marina identifies a blue-light power sensor, J. Biol. Chem., 293(5), 1713-1727
  15. K. Fushimi, M. Ikeuchi, R. Narikawa, (2017) The expanded red/green cyanobacteriochrome lineage: An evolutionary hot spot, Photochem. Photobiol., 93(3), 903-906
  16. K. Fushimi, G. Enomoto, M. Ikeuchi, R. Narikawa, (2017) Distinctive properties of dark reversion kinetics between two red/green-type cyanobacteriochromes and their application in the photoregulation of cAMP synthesis, Photochem. Photobiol., 93(3), 681-691
  17. K. Fushimi, NC. Rockwell, G. Enomoto, N-N-Win, SS. Martin, F. Gan, DA. Bryant, M. Ikeuchi, JC. Lagarias, R. Narikawa, (2016) Cyanobacteriochrome photoreceptors lacking the canonical Cys residue, Biochemistry, 55(50), 6981-6995
  18. K. Fushimi, T. Nakajima, Y. Aono, T. Yamamoto, N-N-Win, M. Ikeuchi, M. Sato, R. Narikawa, (2016) Photoconversion and fluorescence properties of a red/green-type cyanobacteriochrome AM1_C0023g2 that binds not only phycocyanobilin but also biliverdin, Front. Microbiol., 7, 588
  19. R. Narikawa, K. Fushimi, N-N-Win, M. Ikeuchi, (2015) Red-shifted red/green-type cyanobacteriochrome AM1_1870g3 from the chlorophyll d-bearing cyanobacterium Acaryochloris marina, Biochem. Biophys. Res. Commun., 461(2), 390-395
  20. T. Asai, J-H. Choi, T. Ikka, K. Fushimi, N. Abe, H. Tanaka, Y. Yamakawa, H. Kobori, Y. Kiriiwa, R. Motohashi, VK. Deo, T. Asakawa, T. Kan, A. Morita, H. Kawagishi, (2015) Effect of 2-azahypoxanthine (AHX) produced by the fairy-ring-forming fungus on the growth and the grain yield of rice, Jpn. Agric. Res. Quart., 49(1), 45-49
  21. R. Narikawa, T. Nakajima, Y. Aono, K. Fushimi, G. Enomoto, N-N-Win, S. Itoh, M. Sato, M. Ikeuchi, (2015) A biliverdin-binding cyanobacteriochrome from the chlorophyll d-bearing cyanobacterium Acaryochloris marina, Sci. Rep., 5, 7950
  22. R. Narikawa, G. Enomoto, N-N-Win, K. Fushimi, M. Ikeuchi, (2014) A new type of dual-Cys cyanobacteriochrome GAF domain found in cyanobacterium Acaryochloris marina, which has an unusual red/blue reversible photoconversion cycle, Biochemistry, 53(31), 5051-5059
  23. K. Fushimi, K. Anzai, S. Tokuyama, Y. Kiriiwa, N. Matsumoto, A. Sekiya, D. Hashizume, K. Nagasawa, H. Hirai, H. Kawagishi, (2012) Agrocybynes A-E from the culture broth of Agrocybe praecox, Tetrahedron, 68(4), 1262-1265
  24. J. Wu, K. Fushimi, S. Tokuyama, M. Ohno, T. Miwa, T. Koyama, K. Yazawa, K. Nagai, T. Matsumoto, H. Hirai, H. Kawagishi, (2011) Functional-food constituents in the fruiting bodies of Stropharia rugosoannulata, Biosci. Biotechnol. Biochem. 75(8), 1631-1634
  25. K. Fushimi, M. Horikawa, K. Suzuki, A. Sekiya, S. Kanno, S. Shimura, H. Kawagishi, (2010) Applanatines A-E from the culture broth of Ganoderma applanatum, Tetrahedron, 66(48), 9332-9335
  26. J-H. Choi, K. Fushimi, N. Abe, H. Tanaka, S. Maeda, A. Morita, M. Hara, R. Motohashi, J. Matsunaga, Y. Eguchi, N. Ishigaki, D. Hashizume, H. Koshino, H. Kawagishi, (2010) Disclosure of the “Fairy” of fairy-ring-forming fungus Lepista sordida, ChemBioChem, 11(10), 1373-1377
  27. J-H. Choi, N. Abe, H. Tanaka, K. Fushimi, Y. Nishina, A. Morita, Y. Kiriiwa, R. Motohashi, D. Hashizume, H. Koshino, H. Kawagishi, (2010) Plant-growth regulator, imidazole-4-carboxamide produced by fairy-ring forming fungus Lepista sordida, J. Agr. Food Chem., 58(18), 9956-9959

総説・解説等

  1. 伏見 圭司, 成川 礼, シアノバクテリオクロムの進化的系譜から着想を得た多彩な光変換分子の合理的設計, 光合成研究, Vol.30, No.2, 96-109 (2020)
  2. 伏見 圭司, 成川 礼, 光を感知して色が変わるシアノバクテリオクロム, バイオサイエンスとインダストリー, Vol.77, No.6, 436-437 (2019)
  3. 伏見 圭司, 成川 礼, 哺乳類内在性色素を結合するシアノバクテリオクロム, バイオサイエンスとインダストリー, Vol.77, No.6, 472-474 (2019)
  4. K. Fushimi, R. Narikawa, Cyanobacteriochromes: Photoreceptors covering the entire UV-to-visible spectrum, Curr. Opin. Struct. Biol., 39-46 (2019)
  5. 伏見 圭司, 成川 礼, 光合成生物における開環テトラピロール結合型光受容体, 生物物理, Vol.58, No.6, 303-307 (2018)
  6. 伏見 圭司, 成川 礼, 遠赤色光を吸収して光変換しつつ蛍光を発するタンパク質の発見と応用利用, ケミカルエンジニアリング, Vol.61, No.7, 13-17 (2016)

著書

  1. K. Fushimi, R. Narikawa, Phytochromes and cyanobacteriochromes: Photoreceptor Molecules Incorporating a Linear Tetrapyrrole Chromophore, Optogenetics: Light-sensing Proteins and Their Applications in Neuroscience and Beyond (Second Edition), Springer, Chapter 10, 167-187 (2021/01/06)
  2. K. Miyake, K. Fushimi, R. Narikawa, The Diversity of Cyanobacterial Photoresponsive Systems, Green Science and Technology, CRC Press, Chapter 8, 116-126 (2019/11/12)