Members

BioProduction Engineering Lab Members (Sci., Technol. and Innov.) Akihiro Urasaki

Associate ProfessorAkihiro Urasaki

Education and Experience

1999
  • B.S. Department of Applied Biological Sciences, Faculty of Agriculture and Veterinary Medicine, Nihon University, Japan
    • 2001
  • M.S. Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan
    • 2004-2010
  • Postdoctoral Fellow, Division of Developmental Genetics, National Institute of Genetics, Japan
    • 2010-2013
  • Postdoctoral Fellow, Stefan Schulte-Merker group, Hubrecht Institute, The Netherlands
    • 2013-2018
  • Assistant Professor, Laboratory of Systems Neurobiology and Medicine, Division of Biological Science, Nara Institute of Science and Technology, Japan
    • 2018-2023
  • Laboratory Chief, Department of Molecular Physiology, National Cerebral and Cardiovascular Center Research Institute, Japan
    • 2023
  • Project Researcher, Division of Anatomy and Developmental Biology, Department of Anatomy, Kyoto Prefectural University of Medicine, Japan
    • 2023-present
  • Visiting Scholar, Graduate School of Science, Hokkaido University, Japan
    • 2024-2024
  • Project Associate Professor, Graduate School of Science, Technology and Innovation, Kobe University, Japan
    • 2024-2024
  • Project Associate Professor, Engineering Biology Research Center, Kobe University, Japan
    • 2025-present
  • Project Associate Professor, Graduate School of Science, Technology and Innovation, Kobe University, Japan

    • Awards

    • 2007　5th European Zebrafish Genetics and Development Meeting Best Poster Award
    • 2003　日本遺伝学会 Best Paper賞

    Publications

    1. Iwasa, T., Urasaki, A., Kakihana, Y., Nagata-Akaho, N., Harada, Y., Takeda, S., Kawamura, T., Shiraishi, I., Kurosaki, K., Morisaki, H., Yamada, O., Nakagawa, O. (2023/07) Computational and Experimental Analyses for Pathogenicity Prediction of ACVRL1 Missense Variants in Hereditary Hemorrhagic Telangiectasia., Journal of Clinical Medicine, 12(15), 5002
    2. Harada, Y., Tanaka, T., Arai, Y., Isomoto, Y., Nakano, A., Nakao, S., Urasaki, A., Watanabe, Y., Kawamura, T., Nakagawa, O. (2021/06) ETS-dependent enhancers for endothelial-specific expression of serum/glucocorticoid-regulated kinase 1 during mouse embryo development. Genes Cells, 26(8), 611-626
    3. Kinugasa-Katayama, Y., Watanabe, Y., Hisamitsu, T., Arima, Y., M Liu, N., Tomimatsu, A., Harada, Y., Arai, Y., Urasaki, A., Kawamura, T., Saito, Y., Nakagawa, O. (2021/03) Tmem100-BAC-EGFP mice to selectively mark and purify embryonic endothelial cells of large caliber arteries in mid-gestational vascular formation. genesis, 59(4), e23416
    4. Urasaki, A., Morishita, S., Naka,K., Uozumi,M., Abe,K., Huang,L., Watase, E., Nakagawa, O., Kawakami, K., Matsui, T., Bessho, Y., Inagaki, N.(2019/08) Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system., Scientific Reports, 9(1), 12156
    5. Huang, L., Urasaki, A., Inagaki, N. (2019/02) Rab33a and Rab33ba mediate the outgrowth of forebrain commissural axons in the zebrafish brain., Scientific Reports, 9(1), 1799
    6. Higashiguchi, Y., Katsuta, K., Minegishi, T., Yonemura, S., Urasaki, A., Inagaki, N. (2016/05) Identification of a shootin1 isoform expressed in peripheral tissues. Cell and Tissue Research, 366(1), 75-87
    7. Hermkens, D.M., Impel, A. van., Urasaki, A., Bussmann, J., Duckers, H.J., Schulte-Merker, S. (2015/05) Sox7 controls arterial specification in conjunction with hey2 and efnb2 function., Development, 142(9), 1695-1704
    8. Kishimoto, N., Alfaro-Cervello, C., Shimizu, K., Asakawa, K., Urasaki, A., Nonaka, S., Kawakami, K., Garcia-Verdugo, J.M., Sawamoto, K. (2011/12) Migration of neuronal precursors from the telencephalic ventricular zone into the olfactory bulb in adult zebrafish., The Journal of Comparative Neurology, 519(17), 3549-3565
    9. Kawakami, K., Abe, G., Asada, T., Asakawa, K., Fukuda, R., Ito, A., Lal, P., Mouri, N., Muto, A., Suster, M.L., Takakubo, H., Urasaki, A, Wada, H., Yoshida, M. (2010/10) zTrap: zebrafish gene trap and enhancer trap database., BMC Developmental Biology, 10, 105
    10. Bussmann, J., Bos, F.L., Urasaki, A., Kawakami, K., Duckers, H.J., Schulte-Merker, S. (2010/08) Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk., Development, 137(16), 2653-2657
    11. Yagita, K., Horie, K., Koinuma, S., Nakamura, W., Yamanaka, I., Urasaki, A., Shigeyoshi, Y., Kawakami, K., Shimada, S., Takeda, J., Uchiyama, Y. (2010/02) Development of the circadian oscillator during differentiation of mouse embryonic stem cells in vitro. Proceedings of the National Academy of Sciences, 107(8), 3846-3851
    12. Suster, M.L., Kikuta, H., Urasaki, A., Asakawa, K., Kawakami, K. (2009/01) Transgenesis in zebrafish with the Tol2 transposon system., Methods in Molecular Biology, 561, 41-63
    13. Koide, T., Miyasaka, N., Morimoto, K., Asakawa, K., Urasaki, A., Kawakami, K., Yoshihara, Y. (2009/06) Olfactory neural circuitry for attraction to amino acids revealed by transposon-mediated gene trap approach in zebrafish., Proceedings of the National Academy of Sciences, 106(24), 9884-9889
    14. Urasaki, A., Kawakami, K. (2009/01) Analysis of genes and genome by the Tol2-mediated gene and enhancer trap methods., Methods in Molecular Biology, 546, 85-102
    15. Urasaki, A., Asakawa, K., Kawakami, K. (2008/12) Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish., Proceedings of the National Academy of Sciences, 105(50), 19827-19832
    16. Urasaki, A., Mito, T., Noji, S., Ueda, R., Kawakami, K. (2008/12) Transposition of the vertebrate Tol2 transposable element in Drosophila melanogaster., Gene, 425(1-2), 64-68
    17. Asakawa, K., Suster, M.L., Mizusawa, K., Nagayoshi, S., Kotani, T., Urasaki, A., Kishimoto, Y., Hibi, M., Kawakami, K. (2008/01) Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish., Proceedings of the National Academy of Sciences, 105(4), 1255-1260
    18. Nagayoshi, S., Hayashi, E., Abe, G., Osato, N., Asakawa, K., Urasaki, A., Horikawa, K., Ikeo, K., Takeda, H., Kawakami, K. (2008/01) Insertional mutagenesis by the Tol2 transposon-mediated enhancer trap approach generated mutations in two developmental genes: tcf7 and synembryn-like., Development, 135(1), 159-169
    19. Fisher, S., Grice, E.A., Vinton, R.M., Bessling, S.L., Urasaki, A., Kawakami, K., McCallion, A.S. (2006/10) Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish., Nature Protocols, 1(3), 1297-1305
    20. Urasaki, A., Morvan, G., Kawakami, K. (2006/10) Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition., Genetics, 174(2), 639-649
    21. Kotani, T., Nagayoshi, S., Urasaki, A., Kawakami, K. (2006/07) Transposon-mediated gene trapping in zebrafish, Methods, 39(3), 199-206
    22. Urasaki, A., Sekine, Y., Ohtsubo, E. (2002/09) Transposition of cyanobacterium insertion element ISY100 in Escherichia coli., Journal of Bacteriology, 184(18), 5104-5112

    Reviews & Commentaries

    1. 馬場健太郎, 浦﨑明宏, 稲垣直之, ラージゲルプロテオミクスを基盤とした神経細胞の軸索形成とガイダンスの解析, 生物物理化学, 58(2), 49-52 (2014/10)
    2. 鈴木良知, 浦﨑明宏, 浅見幸夫, 井坂恵一, 川上浩一, Tol2トランスポゾンによる子宮内膜腺癌株(イシカワ株)への効果的な遺伝子導入：着床障害不妊症の遺伝子治療に対する有益なDNAベクター, 東京醫科大學雜誌, 68(4), 396-402 (2010/10)
    3. 浦﨑明宏, 浅川和秀, 川上浩一, メダカトランスポゾンTol2が開く新しいゼブラフィッシュ研究, 細胞工学, 28(6), 586-591 (2009/05)
    4. 浦﨑明宏, 川上浩一, 【ゲノム上を"動く遺伝子"トランスポゾン エピジェネティクス・クロマチン動態との関連性から発生・生殖を司る機能まで】 脊椎動物におけるトランスポゾンを用いた遺伝学的方法論, 実験医学, 25(16), 2507-2512 (2007/10)