Members

BioProduction Engineering Lab Members (Sci., Technol. and Innov.) Prihardi Kahar

Associate ProfessorPrihardi Kahar

History

1997/03
静岡大学農学部応用生物化学科　卒業
1999/03
静岡大学大学院農学研究科修士課程生物機能制御化学専攻　修了
2001/04
理化学研究所ジュニア・リサーチ・アソシエイト（常勤）
2002/03
岐阜大学院連合農学研究科博士課程生物資源利用科学専攻　修了
2002/04
科学技術振興機構(JST) 研究員（常勤）
2008/05
明星大学アジア環境研究センター　特任研究員（常勤）
2010/04
明星大学理工学部アジア環境研究プロジェクト　主任研究員（常勤）
2013/04
明星大学理工学部環境・生態学系　研究職員（非常勤）
2013/09
神戸大学大学院工学研究科応用化学専攻　特命助教
2018/04
神戸大学大学院　科学技術イノベーション研究科　特命助教
2018/09
関西化学機械製作株式会社　上級研究員
2019/09
神戸大学大学院　工学研究科　特命准教授
　
2025/04
神戸大学　先端バイオ工学研究センター　特命准教授
2025/07
神戸大学大学院　科学技術イノベーション研究科　特命准教授

Awards

  • 2021年11月　Young Asian Biological Engineer’s community (YABEC)
    　Radityo Pangestu, Prihardi Kahar, Chiaki Ogino
  • 2016年10月　応用化学専攻長感謝状 神戸大学工学研究科応用化学専攻
    　Prihardi Kahar
  • 2004年　　　The Prominent Poster Award, Effective Production and Kinetic Characteristic of Ultra-high-molecular-weight of PHB in Recombinant Escherichia coli, International Symposium on Biological Polyesters (ISBP)
    　Prihardi Kahar
  • 1998年4月　公益財団法人橋谷奨学金
    　Prihardi Kahar
  • 1997年4月　佐藤陽国際奨学金 公益財団法人佐藤陽国際奨学財団
    　Prihardi Kahar
  • 1992年4月　STAID Fellow, Indonesia Government インドネシア政府
    　Prihardi Kahar

Publications

  1. Tasia, W., Kawamoto, K., Morita, K., Mori, Y., Kahar, P., Sasaki, R., Ogino, C. (2025/4). Selective Interaction and Radio-Sensitizing Effect of Rutin-G-Modified Titanium Peroxide Nanoparticles on Breast Cancer Cells. Preprints.org
  2. Rahmasari, D., Kahar, P., de Oliveira, A. V., Putra, F. J. N., Kondo, A., Ogino, C. (2025/3). Factors Affecting D-Lactic Acid Production by Flocculant Saccharomyces cerevisiae Under Non-Neutralizing Conditions. Microorganisms, 13(3), 618.
  3. Tasia, W., Washio, A., Yamate, K., Morita, K., Mori, Y., Kahar, P., Sasaki, R., Ogino, C. (2025/1). Catalase-Knockout Complements the Radio-Sensitization Effect of Titanium Peroxide Nanoparticles on Pancreatic Cancer Cells. Molecules, 30(3), 629.
  4. Zain, N.-A. A., Tan, K. L., Kahar, P., Ogino, C. (2025/1). Lipid Production from Palm Acid Oil (PAO) as a Sole Carbon Source by Meyerozyma guilliermondii. Processes, 13(2), 311.
  5. Apriliana, P., Kahar, P., Rachmadona, N., Restu, W. K., Kondo, A., Ogino, C. (2025/1). Lipid Production in Streptomyces jeddahensis Is Enhanced by Glucose and Fatty Acid Derivatives, with Temperature Variations Influencing Gene Expression and Biosynthesis. Fermentation, 11(2), 45.
  6. Postiaux, A., Putra, F.J.N., Kahar, P., Richel, A., Ogino, C. (2024/11). Maleic Acid-Butanol Pretreatment to Enhance Cellulose Accessibility for Enzymatic Hydrolysis and Ethanol Production from Oil Palm Empty Fruit Bunch. ACS Environmental Au, 5, 1, 76–85.
  7. Rachmadona, N., Kahar, P., Juanssilfero, A.B. et al. (2024/10). Evaluation of lipid production efficiency using palm oil mill effluent as a carbon source by Lipomyces starkeyi. Biomass Conversion and Biorefinery.
  8. Kahar, P. (2024/10). Insights in Synthetic Bioengineering and Aspects of Creating a New Biotechnology. SynBio, 2(4), 344-348.
  9. Putra, F.J.N., Kahar, P., Kondo, A., Ogino, C. (2024/10). Adaptive laboratory evolution of Lipomyces starkeyi for high production of lignin derivative alcohol and lipids with comparative untargeted metabolomics-based analysis. Microbial Cell Factory, 23, 270.
  10. Zain, N.A.A., Kahar, P., Sudesh, K., Ogino, C., Kondo, A. (2024/08) Production of single cell oil by Lipomyces starkeyi from waste plant oil generated by the palm oil mill industry, Journal of Bioscience and Bioengineering, 138(2), 153-162
  11. Koh, S., Endo, R., Kahar, P., Mori, Y., Ogino, C., Tanaka, S., Tanaka, S., Imai, Y., Taguchi, S. (2024/08) Complete sequence randomness of lactate-based copolymers, LAHBs with varied lactate monomer fractions employing a series of propionyl-CoA transferases, International Journal of Biological Macromolecules, 133055
  12. Putra, F.J.N., Kahar, P., Kondo, A., Ogino, C. (2024/08) Production of lignin monomeric alcohols and lipids from oil palm empty fruit bunch by a combination of alkaline nitrobenzene depolymerization and Lipomyces starkeyi bioconversion, Biochemical Engineering Journal, 208, 109347
  13. Apriliana, P., Kahar, P., Kashiwagi, N., Kondo, A., Ogino, C. (2024/06) Editing Streptomyces genome using target AID system fused with UGI-degradation tag, Engineering in Life Sciences, 24:e2400005
    14. 　
  14. Pangestu, R., Kahar, P., Ogino, C., Kondo, A. (2024/04). Comparative responses of flocculating and nonflocculating yeasts to cell density and chemical stress in lactic acid fermentation, Yeast, 41, 192–206
  15. Elkasaby, T., Hanh, D.D., Kahar, P., Kawaguchi, H., Sazuka, T., Kondo, A., Ogino, C. (2024/01) Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum, Enzyme and Microbial Technology, 172, 110345
  16. Wijaya, H., Kahar, P., Sasaki, K., Rahmani, N., Hermiati, E., Yopi, Ogino, C., Prasetya, B., Simarmata, R., Christita, M., Kondo, A. (2023/12) Saccharification of pretreated empty bunch by concentrated xylanase and assisted by cellulase, AIP Conference Proceedings, 2972(1): 040001
  17. Narutaki, A., Kahar, P., Shimadzu, S., Maeda, S., Furuya, T., Ishizaki, K., Fukaki, H., Ogino, C., Kondo, Y. (2023/12) Sucrose signaling contributes to the maintenance of vascular cambium by inhibiting cell differentiation, Plant and Cell Physiology, S, 64(12), 1511–1522
  18. Putra, F.J.N., Kahar, P., Kondo, A., Ogino, C. (2023/11). The bioconversion of lignin derivative aldehydes into high-value aromatic alcohols and lipids via Lipomyces starkeyi, Biochemical Engineering Journal, 200, 109065
  19. Risanto, R., Nugroho Adi, Dt., Fajriutami, T., Teramura, H., Fatriasari, W., Hermiati, E., Kahar, P., Kondo, A., Ogino, C. (2023/02) Pretreatment with dilute maleic acid enhances the enzymatic digestibility of sugarcane bagasse and oil palm empty fruit bunch fiber, Bioresource Technology, 369, 128382
  20. Putra, F.J.N., Kahar, P., Kondo, A., Ogino, C. (2022/10) Valorization of Lignin and Its Derivatives Using Yeast, Processes, 10, 2004
  21. Pangestu, R., Kahar, P., Kholida, LN., Perwitasari, U., Thontowi, A., Fahrurrozi, Lisdiyanti, P., Yopi, Ogino, C., Prasetya, B., Kondo, A. (2022/08) Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization, Scientific Reports, 12, 13645
  22. Kahar, P., Itomi, A., Tsuboi, H., Ishizaki, M., Yasuda, M., Kihira, C., Otsuka, H., Azmi, N.b., Matsumoto, H., Ogino, C., Kondo, A. (2022/07) The flocculant Saccharomyces cerevisiae strain gains robustness via alteration of the cell wall hydrophobicity, Metabolic Engineering, 72, 82-96
  23. Ratnakomala, S., Kahar, P., Kashiwagi, N., Lee, J., Kudou, M., Matsumoto, H., Apriliana, P., Yopi, Y., Prasetya, B., Ogino, C., Kondo, A. (2022/01) Manno-Oligosaccharide Production from Biomass Hydrolysis by Using Endo-1,4-β-Mannanase (ManNj6-379) from Nonomuraea jabiensis ID06-379, Processes, 10, 269
  24. Kahar, P., Rachmadona, N., Pangestu, R., Palar, R., Nugroho Adi, DT., Juanssilfero, AB., Yopi, Manurung, I., Hama, S., Ogino, C.* (2022/01) An integrated biorefinery strategy for the utilization of palm-oil wastes, Bioresource Technology, 344(B): 126266
  25. Kawaguchi, H., Takada, K., Elkasaby, T., Pangestu, R., Toyoshima, M., Kahar, P., Ogino, C., Kaneko, T., Kondo, A. (2022/01) Recent advances in lignocellulosic biomass white biotechnology for bioplastics, Bioresource Technology, 344(B): 126165
  26. Wijaya, H., Sasaki, K., Kahar, P., Rahmani, N., Hermiati, E., Yopi, Ogino, C., Prasetya, B., Kondo, A. (2020/05) High enzymatic recovery and purification of xylooligosaccharides from empty fruit bunch via nanofiltration, Processes, 8, 619
  27. Wijaya, H., Sasaki, K., Kahar, P., Quayson, E., Rachmadona, N., Amoah, J., Hama, S., Ogino, C., Kondo, A. (2020/04) Concentration of lipase from Aspergillus oryzae expressing Fusarium heterosporum by nanofiltration to enhance transesterification, Processes, 8(4), 450
  28. Amza, RL., Kahar, P., Juanssifero, AB., Miyamoto, N., Otsuka, H., Kihira, C., Ogino, C., Kondo, A. (2019/09) High cell density cultivation of Lipomyces starkeyi for achieving highly efficient lipid production from sugar under low C/N ratio, Biochemical Engineering Journal, 149, 107236
  29. Juanssilfero, AB., Kahar, P., Amza, RL., Yopi, Sudesh, K., Ogino, C., Prasetya, B., Kondo, A. (2019/06) Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks, Journal of Bioscience and Bioengineering, 127(6), 726-731
  30. Takenaka, M., Lee, JM., P. Kahar, Ogino, C., Kond, A. (2019/03) Efficient and supplementary enzyme cocktail from Actinobacteria and plant biomass induction, Biotechnology Journal, 1700744 (3月号表紙採用)
  31. Rahmani, N., P. Kahar, Lisdiyanti, P., Lee, J., Yopi., Prasetya, B., Ogino, C., Kondo, A. (2019/01) GH-10 and GH-11 Endo-1,4-β-xylanase enzymes from Kitasatospora sp. produce xylose and xylooligosaccharides from sugarcane bagasse with no xylose inhibition, Bioresource Technology, 272, 315-325
  32. Juanssilfero, BA., P. Kahar, Amza, LR., Miyamoto, N., Otsuka, H., Matsumoto, H., Kihira, C., Thontowi, A., Yopi., Ogino, C., Prasetya, B., Kondo, A. (2018) Selection of oleaginous yeasts capable of high lipid accumulation during challenges from inhibitory chemical compounds, Biochemical Engineering Journal, 137, 182-191
  33. Rahmani, N., P. Kahar, Lisdiyanti, P., Hermiati, Euis, Lee, J., Yopi, Y., Prasetya, B., Ogino, C., Kondo, A. (2018) Xylanase and feruloyl esterase from actinomycetes cultures could enhance sugarcane bagasse hydrolysis in the production of fermentable sugars, Bioscience, Biotechnology, and Biochemistry, 23: 1-12
  34. Wijaya, H., Sasaki, K., P. Kahar, Yopi, Kawaguchi, H., Sazuka, T., Ogino, C., Prasetya, B., Kondo, A. (2018) Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain, Bioresource Technology, 265, 542-547
  35. Juanssilfero, AB., P. Kahar, Amza, RL., Miyamoto, N., Otsuka, H., Matsumoto, H., Kihira, C., Thontowi, A., Yop, Ogino, C., Prasetya, B., Kondo, A. (2018/06) Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi, Journal of Bioscience and Bioengineering, 126(6), 695-702
  36. P. Kahar, E. I. Riyanti, Otsuka, H., Matsumoto, H., Kihira, C., Ogino, C., Kondo, A. (2017) Challenges of non-flocculating Saccharomyces cerevisiae haploid strain against inhibitory chemical complex for ethanol production, Bioresource Technology, 245, 1436-1446
  37. A. Prima, Hara, Y. K., A. C. Djohan, Kashiwagi, N., P. Kahar, Ishii, J., Nakayama, H., Okazaki, F., B. Prasetya, Kondo, A., Yopi, Ogino, C. (2017) Glutathione production from mannan-based bioresource by mannanase/mannosidase expressing Saccharomyces cerevisiae, Bioresource Technology, 245, 1400-1406
  38. Kawaguchi, H., Katsuyama, Y., Danyao, D., P. Kahar, Nakamura-Tsuruta, S., Teramura, H., Wakai, K., Yoshihara K., Minami, H., Ogino, C., Ohnishi, Y., Kondo, A. (2017) Caffeic acid production by simultaneous saccharification and fermentation of kraft pulp using recombinant Escherichia coli, Appl Microbiol Biotechnoliol, 101(13), 5279–5290
  39. N. Rahmani, Kashiwagi, N., Lee, J., Niimi-Nakamura, S., Matsumoto, H., P. Kahar, Lisdiyanti, P., Yopi, Y., Prasetya, B., Ogino, C., Kondo, A. (2017) Mannan endo-1,4-β-mannosidase from Kitasatospora sp. isolated in Indonesia and its potential for production of mannooligosaccharides from mannan polymers, AMB Express, 7(1): 100
  40. Hsu, H., Araki, M., Mochizuki, M., Hori, Y., Murata, M., P. Kahar, Yoshida, T., Hasunuma, T., Kondo, A. (2017) A systematic approach to time-series metabolite profiling and RNA-seq analysis of Chinese hamster ovary cell culture, Scientific Reports, 7:43518
  41. Ishii, J., Okazaki, F., Djohan, C. Apridah., Hara, Y. K., Asai-Nakashima, N., Teramura, H., Andriani, A., Tominaga, M., Wakai, S., P. Kahar, Yopi, Prasetya, B., Ogino, C., Kondo, A. (2016) From mannan to bioethanol: cell surface co-display of β-mannanase and βmannosidase on yeast Saccharomyces cerevisiae., Biotechnology for Biofuels, 9, 188
  42. Kawaguchi, H., Teramura, H., Uematsu, K., Hara, K.Y., Hasunuma, T., Hirano, K., Sazuka, T., Kitano, H., Tsuge, Y., P. Kahar,, Niimi-Nakamura, S., Oinuma, K., Takaya, N., Kasuga, S., Ogino, C., Kondo, A. (2015) Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse., Bioresource Technology, 182:169-178
  43. P. Kahar, Tanaka, S. (2014) A xylose-fermenting yeast hybridized by intergeneric fusion between Saccharomyces cerevisiae and Candida intermedia mutants for ethanol production, Sustainable Chemical Processes, 2(17), 1-12, https://link.springer.com/article/10.1186/s40508-014-0017-y
  44. P. Kahar, Taku, K., Tanaka, S. (2013) Multiple effects of swelling by sodium bicarbonate after delignification on enzymatic saccharification of rice straw, Journal of Bioscience and Bioengineering, 116(6), 725-733, https://www.ncbi.nlm.nih.gov/pubmed/23830033
  45. P. Kahar, Taku, K., Tanaka, S. (2011) Enhancement of xylose uptake in 2-deoxyglucose tolerant mutant of Saccharomyces cerevisiae, Journal of Bioscience and Bioengineering, 111(5), 557-563
  46. J. Agus, P. Kahar, M. Hyakutake, S. Tomizawa, H. Abe, T. Tsuge, Y. Satoh, K. Tajima. (2010) Unusual change in molecular weight of polyhydroxyalkanoate (PHA) during cultivation of PHA-accumulating Escherichia coli, Polymer Degradation and Stability, 95(12), 2250-2254
  47. Febriani, R. Hertadi, P. Kahar, Akhmaloka, F. Madayanti. (2010) Isolation and purification of novel thermostable alkaline lipase from local thermophilic microorganism, Biosciences Biotechnology Research Asia, 7(2), 617-622
  48. P. Kahar, K. Taku, S. Tanaka. (2010) Enzymatic digestion of corncobs pretreated with low strength of sulfuric acid for bioethanol production, Journal of Bioscience and Bioengineering, 110(4), 453-458
  49. J. Agus, P. Kahar, H. Abe, Y. Doi, T. Tsuge. (2006) Altered expression of polyhydroxyalkanoate synthase gene and its effect on poly[(R)-3-hydroxybutyrate] synthesis in recombinant Escherichia coli, Polymer Degradation and Stability, 91(8), 1645-1650
  50. J. Agus, P. Kahar, H. Abe, Y. Doi, T. Tsuge. (2006) Molecular weight characterization of poly[(R)-3-hydroxybutyrate] synthesized by genetically engineered strains of Escherichia coli, Polymer Degradation and Stability, 91(5), 1138-1146
  51. P. Kahar, J. Agus, Y. Kikkawa, K. Taguchi, Y. Doi, T. Tsuge. (2005) Effective Production and Kinetic Characterization of Ultra-High-Molecular-Weight Poly([R]-3-hydroxy butyrate) in Recombinant Escherichia coli, Polymer Degradation and Stability, 87(1), 161-169
  52. P. Kahar, T. Tsuge, K. Taguchi, Y. Doi. (2004) High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain, Polymer Degradation and Stability, 83(1), 79-86
  53. L. Dwiarti, K. Yamane, H. Yamatani, P. Kahar, M. Okabe. (2004) Purification and characterization of cis-aconitic acid decarboxylase from Aspergillus terreus TN484-M1, Journal of Bioscience and Bioengineering, 94(1), 29-33
  54. P. Kahar, K. Kobayashi, T. Iwata, J. Hiraki, M. Kojima, M. Okabe. (2002) Production of ε-polylysine in an airlift bioreactor (ABR), Journal of Bioscience and Bioengineering, 93(3), 274-280
  55. G. Huang, M. Okabe, P. Kahar, H. Tsunekawa, Y. S. Park. (2001) Optimization of tylosin feeding rate profile in production of acetyl-isovaleryl tylosin (AIV) from tylosin by Streptomyces thermotolerans YN554, Journal of Bioscience and Bioengineering, 91(5), 504-508
  56. P. Kahar, T. Iwata, J. Hiraki, E. Y. Park, M. Okabe. (2001) Enhancement of ε-polylysine production by Streptomyces albulus strain 410 using pH control, Journal of Bioscience and Bioengineering, 91(2), 190-194
  57. S. Jia, G. Chen, P. Kahar, D. B. Choi, M. Okabe. (1999) Effect of soybean oil on oxygen transfer in the production of tetracycline with an airlift bioreactor, Journal of Bioscience and Bioengineering, 87(6), 825-827
  58. E. Y. Park, M. Ichida, P. Kahar, M. Okabe. (1999) Kinetics of soybean oil consumption and cephamycin C production in culture of Streptomyces sp. using mineral support, Journal of Bioscience and Bioengineering, 87(3), 390-393
  59. S. Jia, M. Wang, P. Kahar, Y. S. Park, M. Okabe. (1997) Enhancement of yeast fermentation by addition of oxygen vectors in air-lift bioreactor, Journal of Fermentation and Bioengineering, 84(2), 176-178

Reviews & Commentaries

  1. Kahar, P., Zain, N. A. A., Pangestu, R., Ogino, C. Important Key Points Regarding the Circular Bioeconomy in the Palm Oil Industry: Current Practices and Future Perspectives, The Palm Oil Export Market: Trends, Challenges, and Future Strategies for Sustainability, 183-192(2025/3)
    2. 　
  2. Putra, F.J.N., Kahar, P., Ogino C, Kondo. A. Advanced Lignin Valorization for Biorefinery Application, Advanced Lignin Technologies [Working Title], IntechOpen (2024/04)
  3. Kahar, P., Guirimand, G., Hasunuma, T., Ethanol Production by Recombinant CBP Yeasts, Handbook of Biorefinery Research and Technology, Springer, 1-22 (2023/10)
  4. 川口 秀夫, Prihardi Kahar, 荻野 千秋, 草本バイオマスを原料としたバイオリファイナリー利用, 草本バイオマスの増産と利用, アグリバイオ, 510-514 (2023/06)
  5. Kahar, P., Synergistic effects of pretreatment process on enzymatic digestion of rice straw for efficient ethanol fermentation, Environmental Biotechnology, InTech, Chapter 4, 65-87 (2013)
  6. Kahar, P. An optimization of ε-polylysine production by Streptomyces albulus strain, 岐阜大学大学院博士号学術論文 (2002)