Members

BioProduction Engineering Lab Members (Sci., Technol. and Innov.) Akihito Nakanishi

Assistant ProfessorAkihito Nakanishi

Education and Experience

Mar. 2007
BAc, Biological Environment Controlling, Faculty of Agriculture, Kobe University, Japan
Mar. 2009
MAc, Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan
Apr. 2011
Researcher, JSPS Research Fellowship for Young Scientists (DC-2)
Mar. 2012
DAc, Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan
(Withdrawal from school at full term)
Apr. 2012
Technical assistant, Graduate School of Engineering, Kobe University, Japan
May 2012
PhD Degree in Agriculture in Kyoto University
Jun. 2012
Postdoctoral fellow, Graduate School of Engineering, Kobe University, Japan
Apr. 2013
Postdoctoral fellow, Kobe University, Japan
Apr. 2014
Postdoctoral fellow, Kobe University, Japan
Aug. 2015
Postdoctoral fellow, EAD3, National Institute for Agricultural Research, France
Feb. 2018
Assistant professor, School of Bioscience and Biotechnology, Tokyo University of Technology, Japan
Apr. 2024
Project Assistant Professor, Engineering Biology, Kobe University, Japan
Apr. 2025
Project Assistant Professor, Graduate school of Science, Technology and Innovation, Kobe University, Japan

Awards

  • Sep. 2019
    4th East-branch chief award, The Society for Biotechnology, Japan
  • Mar. 2013
    65th Conference Topics Award, The Society for Biotechnology, Japan

Publications

  1. Nakanishi, A., Yomogita, M., Horimoto, T. (2024) Evaluation of cellular responses by Chlamydomonas reinhardtii in media containing dairy-processing residues derived from cheese as nutrients by analyzing cell growth activity and comprehensive gene transcription levels, Microorganisms, 12: 715 (Corresponding author)
  2. Nakanishi, A., Omino, N., Nakamura, T., Goto, S., Matsumoto, R., Yomogita, M., Narisawa, N., Kimijima, M., Iritani, K. (2024) Evaluation of cellular responses of heterotrophic Escherichia coli cultured with autotrophic Chlamydomonas reinhardtii as a nutrient source by analyses based on microbiology and transcriptome, Microorganisms, 12: 452 (Corresponding author)
  3. Nakanishi, A., Mori, M., Yamamoto, N., Nemoto, S., Kanamaru, N., Yomogita, M., Omino, N., Matsumoto, R. (2023) Evaluation of cell responses of Saccharomyces cerevisiae under cultivation using wheat bran as a nutrient resource by analyses of growth activities and comprehensive gene transcription levels, Microorganisms, 11: 2674 (Corresponding author)
  4. Nakanishi, A., Nemoto, S., Yamamoto, N., Iritani, K., Watanabe, M. (2023) Identification of cell-attachment factors derived from green algal cells disrupted by sonication in fabrication of cell plastics, Bioengineering, 10: 893 (Corresponding author)
  5. Iritani, K., Nakanishi, A., Nihei, R., Sugitani, S., Yamashita, T. (2023) Development of epoxy and urethane thermosetting resin using Chlorella sp. as curing agent for materials with low environmental impact, Polymers, 15: 2968 (Corresponding author)
  6. Nakanishi, A., Ozawa, N., Watanabe, M. (2023) Evaluation of shifts of gene transcription levels of unicellular green alga Chlamydomonas reinhardtii due to UV-C irradiation, Microorganisms, 11: 633 (Corresponding author)
  7. Nakanishi, A., Yamamoto, N., Sakihama, Y., Okino, T., Matoba, N. (2023) Development of targeted protein-displaying technology with a novel carbon material, BioTech, 12: 2 (Corresponding author)
  8. Nakanishi, A., Fukunishi, H., Matsumoto, R., Eguchi, F. (2022) Development of a prediction method of cell density in autotrophic/heterotrophic microorganism mixtures by machine learning using absorbance spectrum data, BioTech, 11: 46 (Corresponding author)
  9. Nakanishi, A., Zhang, K., Matsumoto, R., Yamamoto, N. (2022) Estimation of carbon metabolism in Saccharomyces cerevisiae acclimatized to glycerol assimilation with quantitative PCR, Microorganisms, 10: 1173 (Corresponding author)
  10. Nakanishi, A., Iritani, K., Tsuruta, A., Yamamoto, N., Watanabe, M., Ozawa, N., Wanatabe, M., Zhang, K., Tokudome, A. (2022) Fabrication of cell-plastics composed only of unicellular green alga Chlamydomonas reinhardtii as a raw material. Applied Microbiology and Biotechnology, 106: 4459–4468 (Corresponding author)
  11. Nakanishi, A., Ozawa, N., Wanatabe, M., Sakihama, Y. (2021) Evaluation of cell-viability, intracellular lipid-component and efficiency of lipid-extraction of Chlamydomonas reinhardtii cells treated by UV-C irradiation aiming to use cell directly, Austin Journal of Biotechnology & Bioengineering, 8: 1108 (Corresponding author)
  12. Iritani, K., Nakanishi, A., Ota, A., Yamashita, T. (2021) Fabrication of novel functional cell-plastic using polyvinyl alcohol: effects of cross-linking structure and mixing ratio of components on the mechanical and thermal properties, Global Challenges, 5: 2100026
  13. Nakanishi, A., Sakihama, Y., Ozawa N. (2021) Improvement of growth of Chlamydomonas reinhardtii in CO2–stepwisely aerating condition, Journal of Applied Biotechnology Report, 8: 37–40 (Corresponding author)
  14. Nakanishi, A., Iritani K., Sakihama, Y., Watanabe, M., Mochiduki, A., Tsuruta, A., Sakamoto, S., Ota, A. (2021) Fabrication and biodegradability of starch cell-plastics as recyclable resources, Applied Science, 11: 847 (Corresponding author)
  15. Nakanishi, A., Iritani K., Sakihama, Y., Watanabe, M. (2020) Investigation of the mechanical strength of cell-plastics fabricated using unicellular green algal cells and varying weight ratios of biodegradable polybutylene succinate, International Journal of Microbiology and Biotechnology, 5: 159–164 (Corresponding author)
  16. Nakanishi, A., Iritani K., Sakihama, Y., Ozawa, N., Mochizuki, A., Watanabe, M. (2020) Construction of cell-plastics as neo-plastics consisted of cell-layer provided green alga Chlamydomonas reinhardtii covered by two-dimensional polymer. AMB-Express, 10: 112 (Corresponding author)
  17. Amoah, J., Ho, S.H., Hama, S., Yoshida, A., Nakanishi, A., Hasunuma, T., Ogino, C., Kondo, A. (2017) Conversion of Chlamydomonas sp. JSC4 lipids to biodiesel using Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cell as biocatalyst, Algal Research, 28: 16–23
  18. Ho, S.H., Nakanishi, A., Kato, Y., Yamasaki, H., Chang, JS., Misawa, N., Hirose, Y., Minagawa, J., Hasunuma, T., Kondo, A. (2017) Dynamic metabolic profiling together with transcription analysis reveals salinity-induced starch-to-lipid biosynthesis in alga Chlamydomonas sp. JSC4, Scientific Report, 7: 45471
  19. Ishigaki, M., Nakanishi, A., Hasunuma, T., Kondo, A., Morishima, T., Okuno,T., Ozaki, Y. (2017) High-speed scanning for the quantitative evaluation of glycogen concentration in bioethanol feedstock Synechocystis sp. PCC6803 using a near-infrared hyperspectral imaging system with a new near-infrared spectral camera, Applied Spectroscopy, 71: 463–471
  20. Amoah, J., Ho, S.H., Hama, S., Yoshida A., Nakanishi, A., Hasunuma, T., Ogino, C., Kondo, A. (2016) Lipase cocktail for efficient conversion of oils containing phospholipids to biodiesel, Bioresource Technology, 211: 224–230
  21. Amoah, J., Ho, S.H., Hama, S., Yoshida, A., Nakanishi, A., Hasunuma, T., Ogino, C., Kondo, A. (2016) Converting oils high in phospholipids to biodiesel using immobilized Aspergillus oryzae whole-cell biocatalysts expressing Fusarium heterosporum lipase, Biochemical Engineering Journal, 105: 10–15
  22. Ho, S.H., Nakanishi, A., Ye, X., Chang, J-S., Chen, C-Y., Hasunuma, T., Kondo, A. (2015) Dynamic metabolic profiling of the marine microalga Chlamydomonas sp. JSC4 and enhancing its oil production by optimizing light intensity, Biotechnology for Biofuel, 8: 48
  23. Ho, S.H., Nakanishi, A., Ye, X., Chang, J-S., Hara, K., Hasunuma, T., Kondo, A. (2014) Optimizing biodiesel production in marine Chlamydomonas sp. JSC4 through metabolic profiling and an innovative salinity-gradient strategy, Biotechnology for Biofuel, 7: 97
  24. Nakanishi, A., Aikawa, S., Ho, S.H., Chen, C-Y., hang, J-S., Hasunuma, T., Kondo, A. (2014) Development of lipid productivities under different CO2 conditions of marine microalgae Chlamydomonas sp. JSC4, Bioresource Technology, 152: 247–252
  25. Nakanishi, A., Kuroda, K., Ueda, M. (2012) Direct fermentation of newspaper after laccase-treatment using yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase, Renewable Energy, 44: 199–205
  26. Nakanishi, A., Bae, J., Kuroda, K., Ueda, M. (2012) Construction of a novel selection system for endoglucanases exhibiting carbohydrate-binding modules optimized for biomass using yeast cell-surface engineering, AMB-Express, 2: 56
  27. Nakanishi, A., Bae, J., Fukai, K., Tokumoto, N., Kuroda, K., Ogawa, J., Nakatani, M., Shimizu, S., Ueda, M. (2012) Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation, Applied Microbiology and Biotechnology, 94: 939–948
  28. Tamaru, Y., Miyake, H., Kuroda, K., Nakanishi, A., Matsuhima, C., Doi, R.H., Ueda, M. (2011) Comparison of the mesophilic cellulosome-producing Clostridium cellulovorans genome with other cellulosome-related clostridial genomes, Microbial Biotechnology, 4: 64–73
  29. Tamaru, Y., Miyake, H., Kuroda, K., Nakanishi, A., Kawade, Y., Yamamoto, K., Uemura, M., Fujita, Y., Doi, R.H., Ueda, M. (2010) Genome sequence of the cellulosome-producing mesophilic organism Clostridium cellulovorans 743B, Journal of Bacteriology, 192: 901–902

Reviews & Commentaries

  1. 中西 昭仁, 入谷 康平. 第5章 第13節 二酸化炭素を炭素源とした光合成微生物による細胞プラスチックスの開発, CO2の有効利用技術の開発，技術情報協会 (2023/07)
  2. 中西 昭仁, 福西 広晃. AIを用いた微生物混合液の解析手法開発～バイオプラスチックスや食品の発酵生産などの応用にむけて～, 月刊 BIO INDUSTRY, シーエムシー出版 (2023/03)
  3. 中西 昭仁, 入谷 康平. 炭素循環型社会を志向した新規バイオマス材料：細胞プラスチックス, 月刊ファインケミカル, シーエムシー出版 (2023/03)
  4. 中西 昭仁. 農工融合領域における研究から提案する新規プラスチックスの素材について–細胞を用いた新規プラスチックス素材の提案–, 化学と生物, 日本農芸化学会, 59: 62–63 (2021/02)
  5. Nakanishi, A.*,†, Iritani, K.†, Recent progress of cell-plastics as neo bioplastics: a review, Annals of biological research, 12: 58–61 (2021)
  6. Nakanishi, A.*,†, Iritani, K.†, Sakihama, Y. Developing neo-bioplastics for the realization of carbon sustainable society, Journal of Nanotechnology and Nanomaterials, 1: 72–85 (2020)
  7. Nakanishi, A., Yoshida, W., Karube, I.* Organic matter BOD biosensor monitoring, Handbook of Cell Biosensors, 911–927 (2019)
  8. 中西 昭仁. 若手研究者からのメッセージ, バイオテクノロジー部会 NEWS LETTER, 日本科学会, 22: 21–25 (2018)
  9. Aikawa, S., Ho, S.H., Nakanishi, A., Chang, J-S., Hasunuma, T., Kondo, A. Improving polyglucan production in cyanobacteria and microalgae via cultivation design and metabolic engineering, Biotechnology Journal, 10: 886–898 (2015)
  10. Jungu B., 中西 昭仁, 植田 充美, 黒田 浩一 効率的バイオリファイナリーに向けた バイオ前処理技術と耐性育種戦略, 生物工学会誌, 日本生物工学会, 90: 7 (2012)
  11. 中西 昭仁, Jungu, B., 黒田 浩一, 植田 充美, 第5編 セルラーゼの高機能化, 第18章 細胞表層工学を利用した最適なセルラーゼカクテルの構築, バイオマス分解酵素研究の最前線:セルラーゼ・ヘミセルラーゼを中心として, シーエムシー出版, 202–207 (2012)
  12. 中西 昭仁, 黒田 浩一, 植田 充美, 第7章　バイオプロダクトと新プラットフォーム形成, バイオリファイナリーからのフェノールプラットフォーム－フェノール化合物への変換, エコバイオリファイナリー《普及版》脱石油社会へ移行するための環境ものづくり戦略, シーエムシー出版, 229－236 (2010)