People

BioProduction Engineering Lab People (Eng. Biol. Res. C) Hitoshi Mitsunobu

Associate ProfessorHitoshi Mitsunobu

Education and Experience

2005
B.S., Agriculture,　Kyushu University, Fukuoka, Japan
2007
M.S., Agriculture (Molecular Biology),　Kyushu University, Fukuoka, Japan
2010
Ph.D., Agriculture (Molecular Biology),　Kyushu University, Fukuoka, Japan
2009-2016
Postdoctoral Fellow,　Harvard Medical School, Charles C. Richardson Lab Boston, MA
2016-2021
Research assistant professor,　Kobe University, Akihiko Kondo Lab, Hyogo, Japan
2021-2023
Senior scientist, Bio Palette Co., Ltd., Hyogo, Japan
2023-present
Project associate professor, Engineering Biology Research Center, Kobe University

Awards

  • 2006　Heart insect research foundation excellence award

Publications

  1. Mitsunobu, H., Kita, Y., Nambu-Nishida, Y., Miyazaki, S., Nakajima, K., Taoka, K., Kondo, A., Nishida, K. (2025/4) Development of a highly efficient base editing system for Lactobacilli to improve probiotics and dissect essential functions Applied Microbiology and Biotechnology, 109(1), 96
  2. Li, A., Mitsunobu, H., Yoshioka, S., Suzuki, T., Kondo, A., Nishida, K. (2022/8) Cytosine base editing systems with minimized off-target effect and molecular size. Nat Commun., 13(1):4531
  3. Katayama, K., Mitsunobu, H., Nishida, K. (2019/10) Mammalian synthetic biology by CRISPRs engineering and applications, Current Opinion in Chemical Biology 52 79-84
  4. Banno S., Nishida K., Arazoe T., Mitsunobu H., Kondo A. (2018/2) Deaminase-mediated multiplex genome editing in Escherichia coli, Nature Microbiology, 3(4):423-429
  5. Mitsunobu, H., Teramoto, J., Nishida, K., Kondo, A. (2017/10) Beyond Native Cas9: Manipulating Genomic Information and Function. Trends Biotechnol., 35(10):983-996
  6. Zhu, B., Wang, L., Mitsunobu, H., Lu, X., Hernandez, A. J., Yoshida-Takashima, Y., Nunoura, T., Tabor, S., Richardson, C. C. (2017/2) Deep-sea vent phage DNA polymerase specifically initiates DNA synthesis in the absence of primers, Proc Natl Acad Sci U S A., 114(12):E2310-E2318
  7. Li, Z., Mon H., Mitsunobu, H., Zhu, L., Xu, J., Lee, J. M., Kusakabe, T. (2014/12) Dynamics of polycomb proteins-mediated histone modifications during UV irradiation-induced DNA damage. Insect Biochem Mol Biol, 55, 9-18
  8. Mitsunobu, H., Zhu, B., Lee, S. J., Tabor, S., Richardson, C. C. (2014/3) Flap endonuclease of bacteriophage T7: Possible roles in RNA primer removal, recombination and host DNA breakdown. Bacteriophage, 4, e28507
  9. Tatsuke, T., Zhu, L., Li, Z., Mitsunobu, H., Yoshimura, K., Mon, H., Lee, J. M., Kusakabe, T. (2014/2) Roles of Piwi proteins in transcriptional regulation mediated by HP1s in cultured silkworm cells. PLoS One, 9(3), e92313
  10. Mitsunobu, H., Zhu, B., Lee, S. J., Tabor, S., Richardson, C. C. (2014) Flap endonuclease activity of gene 6 exonuclease of bacteriophage T7. J Biol Chem, 289(9), 5860-75
  11. Lee, J. M., Kawakami, N., Mon, H., Mitsunobu, H., Iiyama, K., Ninaki, S., Maenaka, K., Park, E. Y., Kusakabe, T. (2012/6,10) Establishment of a Bombyx mori nucleopolyhedrovirus (BmNPV) hyper-sensitive cell line from the silkworm e21 strain. Biotechnol Lett., 34(10), 1773-9
  12. Mitsunobu, H., Izumi, M., Mon, H., Tatsuke, T., Lee, J. M., Kusakabe, T. (2011/12) Molecular characterization of heterochromatin proteins 1a and 1b from the silkworm, Bombyx mori. Insect Mol Biol., 21(1), 9-20
  13. Mon, H., Izumi, M., Mitsunobu, H., Tatsuke, T., Iiyama, K., Jikuya, H., Lee, J. M., Kusakabe, T. (2011/11) Post-translational modifications of the N-terminal tail of histone H3 in holocentric chromosomes of Bombyx mori. Insect Biochem Mol Biol., 41(11), 902-8
  14. Hong, S. M., Yamashita, J., Mitsunobu, H., Uchino, K., Kobayashi, I., Sezutsu, H., Tamura, T., Nakajima, H., Miyagawa, Y., Lee, J., Mon, H., Miyata, Y., Kawaguchi, Y., Kusakabe, T. (2010/5) Efficient soluble protein production on transgenic silkworms expressing cytoplasmic chaperones. Appl Microbiol Biotechnol., 87(6), 2147-56
  15. Mitsunobu, H., Izumi, M., Iiyama, K., Jikuya, H., Lee, J. M., Mon, H., Kawaguchi, Y., Kusakabe, T. (2010/5) Molecular characterization of core histones in the silkworm, Bombyx mori. J. Insect Biotech Seric., 79, 75-83
  16. Hong, S. M., Noh, S. K., Kim, K. A., Mitsunobu, H., Mon, H., Lee, J. M., Kawaguchi, Y., Kusakabe, T. (2009/5) Molecular characterization, localization, and distribution of innexins in the silkworm, Bombyx mori. Mol Biotechnol., 43(1), 52-8
  17. Lee, J., Takahashi, M., Mon, H., Mitsunobu, H., Koga, K., Kawaguchi, Y., Nakajima, Y., Kusakabe, T. (2007/8) Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori. J Biotechnol., 133(1), 9-17
  18. Sugahara, R., Mon, H., Yamashita, J., Mitsunobu, H., Lee, J., Kawaguchi, Y., Koga, K., and Kusakabe, T. (2007) Heterotrimeric complex of replication protein A, a single-stranded DNA binding protein, from the silkworm, Bombyx mori., J Insect Biotec Seric., 76, 129-135
  19. Yamashita, J., Miyagawa, Y., Sugahara, R., Mon, H., Mitsunobu, H., Lee, J. M., Kawaguchi, Y., and Kusakabe, T. (2007/10) Molecular cloning of silkworm Cdc37 and its interaction with Hsp90 chaperon. ,J Insect Biotech Seric., 76, 137-143
  20. Mitsunobu, H., Sakashita, K., Mon, H., Lee, J. M., Kawaguchi, Y., Koga, K., Kusakabe, T. (2006/10) Construction of gateway-based destination vectors for detecting subcellular localization of proteins in the silkworm, Bombyx mori. J Insect Biotech Seric., 75, 141-6.10.

Books

  1. Ohnishi, M., Mitsunobu, H., Nishida, K., (2021/2) Recent Advances and Applications of Genome Editing Technologies, CMC Publishing CO.,LTD., 技術情報協会, Chapter 5
  2. Li, A., Mitsunobu, H., Nishida, K., (2021/2) ゲノム編集技術を応用した製品開発とその実用化～研究開発動向・課題解決策・技術予測と市場展望, 第3章 5節
  3. Mitsunobu, H., Nishida, K., (2020/10) 遺伝子医学 34 号, メディカル ドゥ, Main theme 2