Members

BioProduction Engineering Lab Members (Sci., Technol. and Innov.) Yuki Kimura

Assistant ProfessorYuki Kimura

Education and Experience

March 2020
Ph.D., Department of Applied Chemistry and Biotechnology, Chiba University, Japan
April 2017–March 2020
JSPS Research Fellow (DC1)
April 2020–March 2021
Postdoctoral fellow, Chiba University, Japan
April 2021–March 2025
Assistant Professor (without tenure), Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, Japan
April 2025–present
Project Assistant Professor, Graduate school of Science, Technology and Innovation, Kobe University, Japan

Publications

  1. Yanai, Y., Tsukada, M., Kimura, Y., Umeno, D.(2025/04) Fabrication, evolution, and mutual conversion of D-fucose-activatable and -repressible acetyltransferase upon mutations, ACS Synthetic Biology, 14(6), 1936–1947
  2. Abe, S., Ayuba, R., Ouchi, K., Tanaka, Y., Fujimoto, A., Kitamura, A., Ogra, Y., Kimura, Y., Umeno, D., Kawai-Noma, S.(2025/04) Enhancement of sensitivity in aggregation-based whole-cell arsenite sensor utilizing arsenic metabolism regulation, ACS Omega, 10(14), 14199–14208
  3. Yanai, Y., Hoshino, T., Kimura, Y., Kawai-Noma, S., Umeno, D.(2024/11) Directed evolution of highly sensitive and stringent choline-induced gene expression controllers, The Journal of General and Applied Microbiology, 70(3), 117-127
  4. Watanabe, T., Kimura, Y., Umeno, D.(2024/07) Systematic promoter design for plasmid–encoded S-adenosylmethionine sensing systems, The Journal of General and Applied Microbiology, 70(1), 51-58
  5. Watanabe, T., Kimura, Y., Umeno, D.(2024/01) MetJ-based mutually interfering SAM-ON/SAM-OFF biosensors, ACS Synthetic Biology, 13(2), 624–633
  6. Kimura, Y., Kawai-Noma, S., Umeno, D.(2023/11) Mutational destabilisation accelerates the evolution of novel sensory and network functions, bioRxiv
  7. Umeno, D., Kimura, Y., Kawai-Noma, S.(2021/05) Transcription factors as evolvable biosensors, Analytical Sciences, 37(5), 699–705
  8. Kimura, Y., Umeno, D.(2020/05) Chapter 8: Directed evolution of transcriptional switches using dual-selector systems, Methods in Enzymology, 644, 191–207
  9. Kimura, Y., Kawai-Noma, S., Saito, K., Umeno, D.(2020/01) Directed evolution of the stringency of the LuxR Vibrio fischeri quorum sensor without OFF-state selection, ACS Synthetic Biology, 9(3), 567–575.
  10. Mu, T., Toyoda, H., Kimura, Y., Yamada, M., Utoh, R., Umeno, D., Seki, M.(2019/12) Laborless, automated microfluidic tandem cell processor for visualizing intracellular molecules of mammalian cells, Analytical Chemistry, 92(3), 2580–2588
  11. Tashiro, M., Ono, K., Kimura, Y., Kawai-Noma, S., Saito, K., Umeno, D.(2018/06) Tweezing the cofactor preference of gymnosperm pinene synthase, Bioscience, Biotechnology, and Biochemistry, 82(6), 1058–1061
  12. Tashiro, Y., Kimura, Y., Furubayashi, M., Tanaka, A., Terakubo, K., Saito, K., Kawai-Noma, S., Umeno, D.(2016/11) Directed evolution of the autoinducer selectivity of Vibrio fischeri LuxR, The Journal of General and Applied Microbiology, 62(5), 240–247
  13. Kimura, Y., Tashiro, Y., Saito, K., Kawai-Noma, S., Umeno, D.(2016/05) Directed evolution of Vibrio fischeri LuxR signal sensitivity, Journal of Bioscience and Bioengineering, 122(5), 533–538

Reviews & Commentaries

  1. 渡邊太郎，木村友紀，梅野太輔，大腸菌におけるSAMバイオセンシングシステム，バイオサイエンスとインダストリー，82(5)，497-499 (2024/09)
  2. 木村友紀，安定性変化を利用したタンパク質機能の集積化, 千葉大学博士論文 (2020/03)
  3. 木村友紀，関貴洋，大谷悠介，栗原健人，梅野太輔，バイオセンサーを利用したハイスループット評価技術，久原哲（監修）スマートセルインダストリー　－微生物細胞を用いた物質生産の展望－，シーエムシー出版， 2(2)，44-50 (2018/06)