Members

BioProduction Engineering Lab Members (Sci., Technol. and Innov. / Eng. Biol. Res. C) Yoichiro Ito

Associate ProfessorYoichiro Ito

Education and Experience

1994
Bc.Eng.　Environmental Chemistry, Saitama University. Urawa, Japan
1996
M.Eng.　Environmental Science, Saitama University, Urawa, Japan
2000
Ph.D　Environmental Science, Saitama University, Urawa, Japan
2000-2009
Postdoctoral Fellow, Biotechnology, Osaka University. Suita, Japan
2009-2014
Visiting Researcher, TOYOTA CENTRAL R & D LABS., INC., Aichi, Japan
2014-2015
Postdoctoral Fellow, Technology Research Association of Highly Efficient Gene Design (TRAHED), Kobe, Japan
2015-2016
Assistant professor, Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan
2016-2020
Assistant professor, Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan
2018-2020
Assistant professor, Engineering Biology Research Center, Kobe University, Kobe, Japan
2020-2021
Postdoctoral Fellow, Technology Research Association of Highly Efficient Gene Design (TRAHED), Kobe, Japan
2021-present
Associate Professor, Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan
Associate Professor, Engineering Biology Research Center, Kobe University, Kobe, Japan

Publications

  1. Asama, R., Tominaga, M., Ito, S., Ito, Y., Takemura, K., Sakuraba, S., Katsurada, K., Fukuda, N., Kondo, A., Ishii, J.(2024/07) Screening of protein-based inhibitors for the intracellular domain of epidermal growth factor receptor by directed evolution using the yeast Gγ recruitment system, Journal of Bioscience and Bioengineering, 138(5), 375-381
  2. Ito, Y., Ishigami, M., Hashiba, N., Nakamura, Y., Terai, G., Hasunuma, T., Ishii, J., Kondo, A. (2022/09) Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris, Microbial Biotechnology, 15(9), 2364-2378
  3. Ito, Y., Ishigami, M., Terai, G., Nakamura, Y., Hashiba, N., Nishi, T., Nakazawa, H., Hasunuma, T., Asai, K., Umetsu, M., Ishii, J., Kondo, A. (2022/06) A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii, Communications Biology, 5, 561
  4. Kumokita, R., Bamba, T., Inokuma, K., Yoshida, T., Ito, Y., Kondo, A., Hasunuma, T. (2022/05) Construction of an ʟ-Tyrosine chassis in Pichia pastoris enhances aromatic secondary metabolites production from glycerol, ACS Synthetic Biology, 11(6), 2098-2107
  5. Nishi, T., Ito, Y., Nakamura, Y., Yamaji, T., Hashiba, N., Tamai, M., Yasohara, Y., Ishii, J.*, Kondo, A. (2022/01) One-step in vivo assembly of multiple DNA fragments and genomic integration in Komagataella phaffii, ACS Synthetic Biology, 11(2), 644-654
  6. Ito, Y., Terai, G., Ishigami, M., Hashiba, N., Nakamura, Y., Bamba, T., Kumokita, R., Hasunuma, T., Asai, K., Ishii, J.*, Kondo, A.* (2020/12) Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression, Nucleic Acids Research, 48(22), 13000-13012
  7. Ito, Y., Watanabe, T., Aikawa, S., Nishi, T., Nishiyama, T., Nakamura, Y., Hasunuma, T., Okubo, Y., Ishii, J., Kondo, A. (2018/11) Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii, FEMS Yeast Research, 18(7), foy074
  8. Nakamura, Y., Nishi, T., Noguchi, R., Ito, Y., Watanabe, T., Nishiyama, T., Aikawa, S., Hasunuma, T., Ishii, J., Okubo, Y., Kondo, A. (2018/07) A stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA, Applied and Environmental Microbiology, 84(15), e02882-17
  9. Inokuma, K., Bamba, T., Ishii, J., Ito, Y., Hasunuma, T., Kondo, A. (2016/11) Enhanced cell-surface display and secretory production of cellulolytic enzymes with Saccharomyces cerevisiae Sed1 signal peptide., Biotechnology and Bioengineering, 113(11), 2358-2366
  10. Ito, Y., Kitagawa, T., Yamanishi, M., Katahira, S., Izawa, S., Irie, K., Furutani-Seiki, M., Matsuyama, T. (2016/11) Enhancement of protein production via the strong DIT1 terminator and two RNA-binding proteins in Saccharomyces cerevisiae, Scientific Reports, 6, 36997
  11. Ito, Y., Yamanishi, M., Ikeuchi, A., Imamura, C., Matsuyama, T. (2015/12) Combinatorial screening for transgenic yeast with high cellulose activities in combination with a tunable expression system, PLoS ONE, e0144870
  12. Ito, Y., Yamanishi, M., Ikeuchi, A., Matsuyama, T. (2015/01) A Highly Tunable System for the Simultaneous Expression of Multiple Enzymes in Saccharomyces cervisiae., ACS Synthetic Biol., 4, 12-16
  13. Ito, Y., Yamanishi, M., Ikeuchi, A., Imamura, C., Tokuhiro, K., Kitagawa, T., Matsuyama, T. (2013/12) Characterization of five terminator regions that increase the protein yield of a transgene in Saccharomyces cerevisiae., J. Biotechnol., 168, 486-492
  14. Yamanishi, M., Ito, Y., Kintaka, R., Imamura, C., Katahira, S., Ikeuchi, A., Moriya, H., Matsuyama, T. (2013/02) A Genome-Wide Activity Assessment of Terminator Region in Saccharomyces cervisiae Provides a “Terminatome” Toolbox., ACS Synthetic Biol., 2, 337-347
  15. Ito, Y., Ikeuchi, A., Imamura, C. (2013/01) Advanced evolutionary molecular engineering to produce thermostable cellulase by using a small but efficient library, Protein Eng. Des. Sel., 26(1), 73-79
  16. Suzuki, M., Tanaka, S., Ito, Y., Inoue, M., Sakai, T., Nishigaki, K. (2012/02) Simple and tunable Förster resonance energy transfer-based bioprobes for high-throughput monitoring of caspase-3 activation in living cells by using flow cytometry, Biochem. Biophys. Acta 1823, 215-226
  17. Matsumoto, Y., Ito, Y., Tsuru, S., Ying, B. W., Yomo, T. (2011/11) Bacterial Cells Carrying Synthetic Dual-Function Operon Survived Starvation, J. Biomed. Biotechnol, Article ID 489265 doi:10.1155/2011/489265
  18. Shimizu, Y., Tsuru, S., Ito, Y., Ying, B. W., Yomo, T. (2011/09) Stochastic Switching Induced Adaptation in a Starved Escherichia coli Population, PLoS ONE, 6(9), e23953
  19. Ying, B.W., Ito, Y., Shimidzu, Y., Yomo, T. (2010/11) Refined method for the genomic integration of complex synthetic circuits, J. Biosci. Bioeng., 110(5), 529-536
  20. Ito, Y., Toyota, H., Kaneko, K., Yomo, T. (2009/04) How selection affects phenotypic fluctuation, Mol. Sys. Biol., 5, 264
  21. Suzuki, M., Ito, Y., Sakata, I., Sakai, T., Husimi, Y., Douglas, K.T. (2005/05) Caspase-3 sensitive signaling in vivo in apoptotic HeLa cells by chemically engineered intramolecular fluorescence resonance energy transfer mutants of green fluorescent protein, Biochem. Biophys. Res. Commun., 330, 454-460
  22. Zhang, W., Suzuki, M., Ito, Y., Douglas, K.T. (2005/04) A Chemically Modified Green-Fluorescent Protein that Responds to Cleavage of an Engineered Disulphide Bond by Fluorescence Resonance Energy Transfer (FRET)-Based Changes, Chem. Lett., 34(6), 766-767
  23. Suzuki, M., Ito, Y., Savage, H.E., Husimi, Y,. Douglas, K.T. (2004/09) Protease-sensitive signalling by chemically engineered intramolecular fluorescent resonance energy transfer mutants of green fluorescent protein, Biochim. Biophys. Acta., 1679, 222-229
  24. Ito, Y., Kawama, T., Urabe, I., Yomo, T. (2004/02) Evolution of an Arbitrary Sequence in Solubility, J. Mol. Evol., 58, 196-202
  25. Sato, K., Ito, Y., Yomo, T., Kaneko, K. (2003/11) On the relation between fluctuation and response in biological systems, PNAS, 100(24), 14086-14090
  26. Suzuki, M., Ito, Y., Savage, H.E., Husimi, Y., Douglas, K.T. (2003/02) Intramolecular Fluorescent Resonance Energy Transfer (FRET) by BODIPY Chemical Modification of Cysteine-engineered Mutants of Green Fluorescent Protein, Chem. Lett., 32(3), 306-307
  27. Ito, Y., Suzuki, M., Husimi, Y. (2000/03) A T-extended vector using a green fluorescent protein as an indicator, Gene, 245, 59-63
  28. Ito, Y., Suzuki, M., Husimi, Y. (1999/10) A Novel Mutant of Green Fluorescent Protein with Enhanced Sensitivity for Microanalysis at 488nm Excitation, Biochem. Biophys. Res. Commun., 264, 556-560

Reviews & Commentaries

  1. 中村泰之, 伊藤洋一郎, 梅津光央, 石井純, 近藤昭彦, 低分子抗体医薬の開発展望, バイオインダストリー, Vol.34, No.6, 54-62 (2017/06)