Members

BioProduction Engineering Lab Members (Eng. Biol. Res. C) Tomohisa Hasunuma

ProfessorTomohisa Hasunuma

Education and Experience

Mar. 1998
B.S. Department of Biotechnology, Graduate School of Engineering, Osaka University
Mar. 2000
M.S. Department of Biotechnology, Graduate School of Engineering, Osaka University
2004/03
Ph.D. Department of Biotechnology, Graduate School of Engineering, Osaka University
2004-2008
Researcher, Research Institute of Innovative Technology for the Earth (RITE)
2008-2009
Assitant Professor, Department of Chemical Science and Engineering, Kobe University
2009-2012
Lecturer, Organization of Advanced Science and Technology, Kobe University
2011-2014
Researcher of Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency
2012-2015
Associate Professor, Organization of Advanced Science and Technology, Kobe University
2015-2016
Professor, Organization of Advanced Science and Technology, Kobe University
2016-present
Professor, Graduate School of Science, Technology and Innovation, Kobe University
2018-present
Director, Engineering Biology Research Center, Kobe University

Research Interests

  • Metabolic Engineering of Microorganisms Based on Metabolomics for Bio-based Chemical Production
  • Development of Omics Platform
  • Photosynthetic Production of Functional Chemicals

Awards

  • Sep. 2025　Biotechnology Paper Award of the Society for Biotechnogy, Japan
  • Oct. 2024　Kobe University President’s Award
  • Oct. 2023　Kobe University President’s Award
  • Oct. 2022　Kobe University President’s Award
  • Oct. 2021　Kobe University President’s Award
  • Oct. 2020　Kobe University President’s Award
  • Oct. 2019　Kobe University President’s Award
  • Oct. 2018　Kobe University President’s Award
  • Oct. 2017　Kobe University President’s Award
  • Sep. 2014　Biotechnology Encouragement Prize of the Society for Biotechnogy, Japan
  • Oct. 2013　Fermentation and Metabolism Research Prize of Japan Bioindustry Association

Publications

  1. Hidese, R., Sakai, K., Takenaka, M., Fushimi, K., Kudo, H., Tanaka, K., Nasuno, R., Vavricka, CJ.,Kondo, A., Hasunuma, T. (2025/06) Identification of subfamily specific residues within highly active and promiscuous alcohol dehydrogenases, ACS Catalysis, 15, 11931-11943
  2. Chen, J., Vavricka, CJ., Wei, S., Nakazawa, Y., Matsumoto, Y., Chen, H., Tang, Y., Liang, J., Chen, J., Huang, Y., Noguchi, K., Hasunuma, T., Guan, H., Li, J., Liao, C., Han, Q. (2025/5)
    3,4-Dihydroxyphenylacetaldehyde synthase evolved an ordered structure to deliver oxygen to pyridoxal 5'-phosphate for cuticle assembly in the mosquito Aedes aegypti, Nature Communications, 16, 4486
  3. Kato, Y., Kamasaka, K., Matsuda, M., Koizumi, H., Ohbayashi, R., Ashida, H., Kondo, A., Hasunuma, T. (2025/3) Metabolic analysis reveals the contribution of mechanosensitive channel MscM to extracellular release of glutamate in glycogen-deficient Synechococcus elongatus, Journal of Bioscience and Bioengineering, 139(3), 187-193
  4. Tanaka, K., Lan, J.CW., Kondo, A., Hasunuma T.* (2025/03) Metabolic engineering and cultivation strategies for efficient production of fucoxanthin and related carotenoids. Applied Microbiology and Biotechnology, 109, 57
  5. Wang, Z., Zeng, D., Zhu, Y., Zhou, M., Kondo, A., Hasunuma, T., Zhao, X. (2025/02) Fermentation design and process optimization strategy based on machine learning ML-BASED FERMENTATION DESIGN AND OPTIMIZATION, BioDesign Research, 100002
  6. Tanaka, K., Yukawa, T., Bamba T., Wakiya, M., Kumokita, R., Jin Y.S., Kondo, A., Hasunuma, T.*(2025/01) Engineering Saccharomyces cerevisiae for growth on xylose using an oxidative pathway, Applied Microbiology and Biotechnology, 109, 30
  7. Aoki, R., Kumagawa, E., Kamata, K., Ago, H., Sakai, N., Hasunuma, T., Taoka, N., Ohta, Y., Kobayash, S. (2025/01) Engineering of acyl ligase domain in nonribosomal peptide synthetases to change fatty acid moieties of lipopeptides, Communications Chemistry, 8:17
  8. Bamba, T., Munakata, R., Ushiro, Y., Kumokita, R., Tanaka, S., Hori, Y., Kondo, A., Yazaki, K., Hasunuma, T. (2024/12) De novo production of the bioactive phenylpropanoid artepillin C using membrane-bound prenyltransferase in Komagataella phaffii. ACS Synthetic Biology, 13(12):4040-4049
  9. Hasunuma, T., Jin, YS. (2024/12) Editorial overview: Chemical biotechnology paving the way for a sustainable future, Current Opinion in Biotechnology, 90, 103215
  10. Haraguchi, Y., Kato, Y., Tsuji, A., Hasunuma, T., Shimizu, T. (2024/10) Recombinant lactate-assimilating cyanobacteria reduce high-concentration culture-associated cytotoxicity in mammalian cells, Archives of Microbiology, 206(11),425
  11. Tsuji, A., Inabe, K., Hidese, R., Kato, Y., Domingues, L., Kondo, A., Hasunuma, T. (2024/10) Pioneering precision in markerless strain development for Synechococcus sp. PCC 7002, Microbial Cell Factories, 23,268
  12. Tanaka, K.*, Kondo, A., Hasunuma, T.* (2024/09) Minimized dark consumption of Calvin cycle intermediates facilitates the initiation of photosynthesis in Synechocystis sp. PCC 6803, Plant Cell Physiology, pcae102
  13. Chu, S., Haraguchi, Y., Asahi, T., Kato, Y., Kondo, A., Hasunuma, T., Shimizu, T. (2024/08) A serum-free culture medium production system by co-culture combining growth factor-secreting cells and l-lactate-assimilating cyanobacteria for sustainable cultured meat production, Scientific Reports, 14: 19578
  14. Inokuma, K., Toyohara, K., Hamada, T., Kondo, A., Hasunuma, T. (2024/08) One-pot synthesis of cellobiose from sucrose using sucrose phosphorylase and cellobiose phosphorylase co-displaying Pichia pastoris as a reusable whole-cell biocatalyst, Scientific Reports, 14: 18540
  15. Mori, H., Matsui, M., Bamba, T., Hori, Y., Kitamura, S., Toya, Y., Hidese, R., Yasueda, H., Hasunuma, T., Shimizu, H., Taoka, N., Kobayashi, S. (2024/07) Engineering Escherichia coli for efficient glutathione production, Metabolic engineering, 84, 180-190
  16. Bamba, T., Aoki, R., Hori, Y., Ishikawa, S., Yoshida, KI., Taoka, N., Kobayashi, S., Yasueda, H., Kondo, A., Hasunuma, T. (2024/06) High-throughput evaluation of hemolytic activity through precise measurement of colony and hemolytic zone sizes of engineered Bacillus subtilis on blood agar, Biology Methods & Protocols, 9(1):bpae044

  17. Takenaka, M., Kamasaka, K., Daryong, K., Tsuchikane, K., Miyazawa, S., Fujihana, S., Hori, Y., Vavricka, CJ., Hosoyama, A., Kawasaki, H., Shirai, T., Araki, M., Nakagawa, A., Minami, H., Kondo, A., Hasunuma, T. (2024/06) Integrated pathway mining and selection of an artificial CYP79-mediated bypass to improve benzylisoquinoline alkaloid biosynthesis, Microbial cell factories, 23, 178
  18. Yoshida, E., Kato, Y., Kanamoto, A., Kondo, A., Hasunuma, T. (2024/05) Mixotrophic culture enhances fucoxanthin production in the haptophyte Pavlova gyrans, Applied Microbiology and Biotechnology, 108, 352
  19. Kato, Y., Hidese, R., Matsuda, M., Ohbayashi, R., Ashida, H., Kondo, A., Hasunuma, T. (2024/02) Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria, Communications Biology, 7, 233
  20. Tanaka, K., Bamba, T., Kondo, A., Hasunuma, T. (2024/02) Metabolomics-based development of bioproduction processes toward industrial-scale production. Current Opinion in Biotechnology, 85, 103057
  21. Kumokita, R., Bamba, T., Yasueda, H., Tsukida, A., Nakagawa, K., Kitagawa, T., Yoshioka, T., Matsuyama, H., Yamamoto, Y., Maruyama, S., Hayashi, T., Kondo, A., Hasunuma,T. (2024/02) High-level phenol bioproduction by engineered Pichia pastoris in glycerol fed-batch fermentation using an efficient pertraction system, Bioresource Technology, 393, 130144
  22. Yuan, B., Wang, WB., Wang, XQ., Liu, CG., Hasunuma, T., Kondo, A., Zhao, XQ. (2024/02) The chromatin remodeler Ino80 regulates yeast stress tolerance and cell metabolism through modulating nitrogen catabolite repression, International Journal of Biological Macromolecules, 258, 129041
  23. Yanagibashi, S., Bamba, T., Kirisako, T., Kondo, A., Hasunuma, T. (2024/01) The potency of mitochondria enlargement for mitochondria-mediated terpenoid production in yeast, Applied Microbiology and Biotechnology, 108: 110
  24. Yanagibashi, S., Bamba, T., Kirisako, T., Kondo, A., Hasunuma, T. (2024/01) Beneficial effect of optimizing the expression balance of the mevalonate pathway introduced into the mitochondria on terpenoid production in Saccharomyces cerevisiae, Journal of Bioscience and Bioengineering, 137(1): 16-23
  25. Hidese, R., Ohbayashi, R., Kato, Y., Matsuda, M., Tanaka, K., Imamura, S., Ashida, H., Kondo, A., Hasunuma, T. (2023/12) ppGpp accumulation reduces the expression of the global nitrogen homeostasis-modulating NtcA regulon by affecting 2-oxoglutarate levels, Communications Biology, 6, 1285
  26. Uy, Abigail Loren Tung., Yamamoto, A., Matsuda, M., Hasunuma, T., Demura, T., Ohtani, M. (2023/12) The carbon flow shifts from primary to secondary metabolism during xylem vessel cell differentiation in Arabidopsis thaliana, Plant and Cell Physiology, 64, 1563-1575
  27. Zhao, S., Zhang, T., Hasunuma, T., Kondo, A., Zhao, XQ., Feng, JX. (2023/11) Every road leads to Rome: diverse biosynthetic regulation of plant cell wall-degrading enzymes in filamentous fungi Penicillium oxalicum and Trichoderma reesei, Critical Reviews in Biotechnology, 1-21
  28. Bamba, T., Hori, Y., Umebayashi, K., Soh, C., Hakozaki, T., Toyama, K., Osumi, M., Kondo, A., Hasunuma, T. (2023/11) Comprehensive metabolic profiling of Geotrichum candidum and comparison with Saccharomyces cerevisiae, Journal of Bioscience and Bioengineering, 137(1): 9-15
  29. Uemura, M., Maeshige, N., Yamaguchi, A., Ma, X., Matsuda, M., Nishimura, Y., Hasunuma, T., Inoue, T., Yan, J., Wang, J., Kondo, H., Fujino, H. (2023/10) Electrical stimulation facilitates NADPH production in pentose phosphate pathway and exerts an anti-inflammatory effect in macrophages, Scientific Reports, 13(1): 17819
  30. Kumokita, R., Yoshida, T., Shirai, T., Kondo, A., Hasunuma, T. (2023/9) Aromatic secondary metabolite production from glycerol was enhanced by amino acid addition in Pichia pastoris , Applied Microbiology and Biotechnology, 107(24): 7391–7401
  31. Haraguchi, Y., Kato, Y., Inabe, K., Kondo, A., Hasunuma, T., Shimizu, T. (2023/06) Circular cell culture for sustainable food production using recombinant lactate-assimilating cyanobacteria that supplies pyruvate and amino acids, Archives of Microbiology, 205(7), 266
  32. Yoshida, E., Kato, Y., Kanamoto, A., Kondo, A., Hasunuma, T. (2023/05) Metabolomic analysis of the effect of nitrogen on fucoxanthin synthesis by the haptophyte Pavlova gyrans. Algal Research, 72, 103144
  33. Kato, Y., Inabe, K., Haraguchi, Y., Shimizu, T., Kondo, A., Hasunuma, T. (2023/05) L-Lactate treatment by photosynthetic cyanobacteria expressing heterogeneous L-lactate dehydrogenase, Scientific Reports, 13(1): 7249
  34. Inokuma, K., Miyamoto, S., Morinaga, K., Kobayashi, Y., Kumokita, R., Bamba, T., Ito, Y., Kondo, A., Hasunuma, T. (2023/04) Direct production of 4-hydroxybenzoic acid from cellulose using cellulase-displaying Pichia pastoris, Biotechnology and Bioengineering, 120(4): 1097-1107
  35. Tanaka, K., Shirai, T., Vavricka, CJ., Matsuda, M., Kondo, A., Hasunuma, T. (2023/04) Dark accumulation of downstream glycolytic intermediates initiates robust photosynthesis in cyanobacteria, Plant Physiology, 191(4), 2400-2413
  36. Yukawa, T., Bamba, T., Matsuda, M., Yoshida, T., Inokuma, K., Kim, J., Lee. J.W., Jin, Y.S., Kondo, A., Hasunuma, T. (2023/02) Enhanced production of 3,4-dihydroxybutyrate from xylose by engineered yeast via xylonate re-assimilation under alkaline condition, Biotechnology and Bioengineering, 120(2): 511-523
  37. Alfaro-Sayes, DA., Amoah, J., Rachmadona, N., Hama, S., Hasunuma, T., Kondo, A., Ogino, C. (2023/01) Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads, Journal of Physics: Energy, 5, 014019
  38. Takenaka, M., Yoshida, T., Yoshida, E., Ono, S., Horie, S., Vavricka, CJ., Hiratani, M., Tsuge, K., Ishii, J., Hayakawa, Y., Kondo, A., Hasunuma, T. (2023/01) Online SFE-SFC-MS/MS colony screening: a high-throughput approach for optimizing (-)-limonene production, Journal of Chromatography B, 1215, 123588
  39. Alfaro-Sayes, D.A., Amoah, J., Aikawa, S., Matsuda, M., Hasunuma, T., Kondo, A., Ogino, C. (2022/12) Alginate immobilization as a strategy for improving succinate production during autofermentation using cyanobacteria Synechocystis sp. PCC 6803, Biochemical Engineering Journal, 188, 108681
  40. Hidese, R., Matsuda, M., Kajikawa, M., Osanai, T., Kondo, A., Hasunuma, T. (2022/11) Metabolic and microbial community engineering for four-carbon dicarboxylic acid production from CO2-derived glycogen in the Cyanobacterium Synechocystis sp. PCC6803, ACS Synthetic Biology, 11, 4054-4064
  41. Zhang, S., Guo, F., yang, Q., Jiang, Y., Yang, S., Ma, J., Xin, F., Hasunuma, T., Kondo, A. Zhang, W., Jiang, M. (2022/10) Improving methanol assimilation in Yarrowia lipolytica via systematic metabolic engineering combined with compartmentalization, Green Chemstry, 25, 183-195
  42. Oyama, T., Kato, Y., Hidese, R., Matsuda, M., Matsutani, M., Watanabe, S., Kondo, A., Hasunuma, T. (2022/09) Development of a stable semi-continuous lipid production system of an oleaginous Chlamydomonas sp. mutant using multi-omics profiling, Biotechnology for Biofuels and Bioproducts, 15, 95
  43. Chetty, BJ., Inokuma, K., Hasunuma, T., van Zyl, WH., den Haan, R. (2022/09) Improvement of cell-tethered cellulase activity in recombinant strains of Saccharomyces cerevisiae, Applied Microbiology and Biotechnology, 106(18), 6347-6361
  44. Ito, Y., Ishigami, M., Hashiba, N., Nakamura, Y., Terai, G., Hasunuma, T., Ishii, J., Kondo, A. (2022/09) Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris, Microbial Biotechnology, 15(9), 2364-2378
  45. Kobayashi, J., Sasaki, D., Hara, KY., Hasunuma, T., Kondo, A. (2022/08) Metabolic engineering of the L-serine biosynthetic pathway improves glutathione production in Saccharomyces cerevisiae, Microbial Cell Factories, 21, 153
  46. Ito, Y., Ishigami, M., Terai, G., Nakamura, Y., Hashiba, N., Nishi, T., Nakazawa, H., Hasunuma, T., Asai, K., Umetsu, M., Ishii, J., Kondo, A. (2022/06) A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii, Communications Biology, 5, 561
  47. Kumokita, R., Bamba, T., Inokuma, K., Yoshida, T., Ito, Y., Kondo, A., Hasunuma, T.* (2022/05) Construction of an ʟ-Tyrosine chassis in Pichia pastoris enhances aromatic secondary metabolites production from glycerol, ACS Synthetic Biology, 11(6), 2098-2107
  48. Yamaguchi, A., Maeshige, N., Ma, X., Uemura, M., Noguchi, H., Matsuda, M., Nishimura, Y., Hasunuma, T., Kondo, H., Fujino, H. (2022/04) Pulsed-ultrasound irradiation induces the production of itaconate and attenuates inflammatory responses in macrophages, Journal of Inflammation Research, 15, 2387-2395
  49. Vavricka, CJ., Takahashi, S., Watanabe, N., Takenaka, M., Matsuda, M., Yoshida, T., Suzuki, R., Kiyota, H., Li, J., Minami, H., Ishii, J., Tsuge, K., Araki, M., Kondo, A., Hasunuma, T. (2022/03) Machine learning discovery of missing links that mediate alternative branches to plant alkaloids, Nature Communications, 13, 1405
  50. Bamba, T.*, Guirimand, G.*, Kondo, A., Hasunuma, T. (2022/02) Enzyme display technology for lignocellulosic biomass valorization by yeast cell factories, Current Opinion in Green and Sustainable Chemistry, 33: 100584
  51. Li, YH., Zhang, P., Zhu, DY., Yao, B., Hasunuma, T., Kondo, A., Zhao, XQ. (2022/01) Efficient preparation of soluble inducer for cellulase production and saccharification of corn stover using in-house generated crude enzymes, Biochemical Engineering Journal, 178, 108296
  52. Chen, HQ., Zhang, MM., Xing, Q., Ye, PL., Hasunuma, T., Kondo, A., Zhao, XQ. (2022/01) Improved ethanol fermentation by promoter replacement of zinc responsive genes IPL1, PRP6 and RTC1 in Saccharomyces cerevisiae, Biochemical Engineering Journal, 178, 108274
  53. Kobayashi, Y., Inokuma, K., Matsuda, M., Kondo, A., Hasunuma, T. (2022/01) Resveratrol production of a recombinant Scheffersomyces stipitis strain from molasses, Biotechnology Notes, 3, 1-7
  54. Kato, Y., Inabe, K., Hidese, R., Kondo, A., Hasunuma, T. (2022/01) Metabolomics-based engineering for biofuel and bio-based chemical production in microalgae and cyanobacteria: A review, Bioresource Technology, 344(A): 126196
  55. Ismail, KSK., Matano, Y., Sakihama, Y., Inokuma, K., Nambu, Y., Hasunuma, T.*, Kondo, A. (2022/01) Pretreatment of extruded Napier grass by hydrothermal process with dilute sulfuric acid and fermentation using a cellulose-hydrolyzing and xylose-assimilating yeast for ethanol production, Bioresource Technology, 343, 126071
  56. Kawaguchi, H., Hasunuma, T., Ohnishi, Y., Sazuka, T., Kondo, A., Ogino, C. (2021/12) Enhanced production of γ-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation, Microbial Cell Factories, 20: 228
  57. Oyama, T., Kato, Y., Satoh, K., Oono, Y., Matsuda, M., Hasunuma, T.*, Kondo, A. (2021/12) Development of mutant microalgae that accumulate lipids under nitrate-replete conditions, Algal Research, 60, 102544
  58. Inabe, K., Miichi, A., Matsuda, M., Yoshida, T., Kato, Y., Hidese, R., Kondo, A., Hasunuma, T. (2021/12) Nitrogen availability affects the metabolic profile in Cyanobacteria, Metabolites, 11, 867
  59. Kobayashi, Y., Inokuma, K., Matsuda, M., Kondo, A., Hasunuma, T. (2021/12) Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain, Metabolic Engineering Communications, 13: e00188
  60. Zhang, C., Hasunuma, T., Lam, SS., Kondo, A., Ho, SH. (2021/11) Salinity-induced microalgal-based mariculture wastewater treatment combined with biodiesel production, Bioresource Technology, 340, 125638
  61. Cunha, JT. Gomes, DG. Romaní, A., Inokuma, K., Hasunuma, T., Kondo, A., Domingues, L. (2021/09) Cell surface engineering of Saccharomyces cerevisiae for simultaneous valorization of corn cob and cheese whey via ethanol production, Energy Conversion and Management, 243, 114359
  62. Inokuma, K., Kitada, Y., Bamba, T., Kobayashi, Y., Yukawa, T., den Haan, R., van Zyl, W. H., Kondo, A., Hasunuma, T.* (2021/08) Improving the functionality of surface-engineered yeast cells by altering the cell wall morphology of the host strain, Applied Microbiology and Biotechnology, 105(14-15): 5895-5904
  63. Chen, JH., Kato, Y., Matsuda, M., Chen, CY., Nagarajan, D., Hasunuma, T., Kondo, A., Chang, JS. (2021/08) Lutein production with Chlorella sorokiniana MB-1-M12 using novel two-stage cultivation strategies - metabolic analysis and process improvement, Bioresource Technology, 334, 125200
  64. Xu, M., Harmon, J., Yuan, D., Yan, S., Lei, C., Hiramatsu, K., Zhou, Y., Loo, MH., Hasunuma, T., Isozaki, A., Goda, K. (2021/06) Morphological Indicator for Directed Evolution of Euglena gracilis with a High Heavy Metal Removal Efficiency, Environmental Science and Technology, 55(12): 7880-7889
  65. Isogai, S., Okahashi, N., Asama, R., Nakamura, T., Hasunuma, T., Matsuda, F., Ishii, J.*, Kondo, A.* (2021/06) Synthetic production of prenylated naringenins in yeast using promiscuous microbial prenyltransferases, Metabolic Engineering Communications, 12, e00169
  66. Ogawa, S., Shimidzu, H., Fukuda, K., Tsunekawa, N., Hirano, T., Sato, F., Yura, K., Hasunuma, T., Ochi, K., Yamamoto, M., Sakamoto, W., Hashimoto, K., Ogata, H., Kanao, T., Nemoto, M., Inagaki, K., Tamura, T. (2021/05) Multiple mutations in RNA polymerase β-subunit gene rpoB in Streptomyces incarnatus NRRL8089 enhance production of antiviral antibiotic sinefungin: Modeling rif cluster region by density functional theory, Bioscience, Biotechnology, and Biochemistry, 85(5), 1275-1282
  67. Guirimand, G., Kulagina, N., Papon, N., Hasunuma, T.*, Courdavault, V.* (2021/05) Innovative tools and strategies for optimizing yeast cell factories, Trends in Biotechnology, 39(5), 488-504
  68. Kato, Y., Oyama, T., Inokuma, K., Vavricka, CJ., Matsuda, M., Hidese, R., Satoh, K., Oono, Y., Chang, JS., Hasunuma, T.*, Kondo, A. (2021/04) Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching enzyme, Communications Biology, 4: 450
  69. Kanamoto, A., Kato, Y., Yoshida, E., Hasunuma, T.*, Kondo, A. (2021/03) Development of a method for fucoxanthin production using the haptophyte marine microalga Pavlova sp. OPMS 30543, Marine Biotechnology, 23, 331-341 (4月号表紙採用)
  70. Shibata, N., Kakeshita, H., Igarashi, K., Takimura, Y., Shida, Y., Ogasawara, W., Koda, T., Hasunuma, T.*, Kondo, A. (2021/02) Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose, Biotechnology for Biofuels, 14(1): 39
  71. Takenaka, M., Yoshida, T., Hori, Y., Bamba, T., Mochizuki, M., Vavricka, CJ., Hattori, T., Hayakawa, Y., Hasunuma, T.*, Kondo, A. (2021/01) An ion-pair free LC-MS/MS method for quantitative metabolite profiling of microbial bioproduction systems, Talanta, 222, 121625
  72. Yukawa, T., Bamba, T., Guirimand, G., Matsuda, M., Hasunuma, T.*, Kondo, A*. (2021/01) Optimization of 1,2,4-butanetriol production from xylose in Saccharomyces cerevisiae by metabolic engineering of NADH/NADPH balance, Biotechnology and Bioengineering, 118(1), 175-185
  73. Yamamoto, H., Hayakawa, E., Tsugawa, H., Moriya, Y., Fukusaki, E., Goto, S., Hasunuma, T., Miura, N., Yoshizawa, A,C. (2020/12) Japan Computational Mass Spectrometry Meeting 2020 Activity Report, Journal of Proteome Data and Methods, 2: 2020. 0005
  74. Ito, Y., Terai, G., Ishigami, M., Hashiba, N., Nakamura, Y., Bamba, T., Kumokita, R., Hasunuma, T., Asai, K., Ishii, J.*, Kondo, A.* (2020/12) Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression, Nucleic Acids Research, 48(22), 13000-13012
  75. Cunha, JT., Romani, A., Inokuma, K., Johansson, B., Hasunuma, T., Kondo, A., Domingues, L. (2020/12) Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts, Biotechnology for Biofuel, 13(1), 1-15
  76. Nitta, N., Iino, T., Isozaki, A., Yamagishi, M., Kitahama, Y., Sakuma, S., Suzuki, Y., Tezuka, H., Oikawa, M., Arai, F., Asai, T., Deng, D., Fukuzawa, H., Hase, M., Hasunuma, T., Hayakawa, T., Hiraki, K., Hiramatsu, K., Hoshino, Y., Inaba, M., Inoue, Y., Ito, T., Kajikawa, M., Karakawa, H., Kasai, Y., Kato, Y., Kobayashi, H., Lei, C., Matsusaka, S., Mikami, H., Nakagawa, A., Numata, K., Ota, T., Sekiya, T., Shiba, K., Shirasaki, Y., Suzuki, N., Tanaka, S., Ueno, S., Watarai, H., Yamano, T., Yazawa, M., Yonamine, Y., Di Carlo, D., Hosokawa, Y., Uemura, S., Sugimura, T., Ozeki, Y., Goda, K. (2020/07) Raman image-activated cell sorting, Nature Communications, 11, 3452
  77. Qu, W., Show, PL., Hasunuma, T., Ho, SH. (2020/06) Optimizing real swine wastewater treatment efficiency and carbohydrate productivity of newly microalga Chlamydomonas sp. QWY37 used for cell-displayed bioethanol production, Bioresource Technology, 305, 123072
  78. Isozaki, A., Nakagawa, Y., Loo, MH., Shibata, Y., Tanaka, N., Setyaningrum, DL., Park, JW., Shirasaki, Y., Mikami, H., Huang, D., Tsoi, H., Riche, CT., Ota, T., Miwa, H., Kanda, Y., Ito, T., Yamada, K., Iwata, O., Suzuki, K., Ohnuki, S., Ohya, Y., Kato, Y., Hasunuma, T., Matsusaka, S., Yamagishi, M., Yazawa, M., Uemura, S., Nagasawa, K., Watarai, H., Di Carlo, D., Goda, K. (2020/05) Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments, Science Advances, 6(22), eaba6712
  79. Hidese, R.#, Matsuda, M.#, Osanai, T., Hasunuma, T.*, Kondo, A. (2020/01) Malic enzyme facilitates D-lactate production through increased pyruvate supply during anoxic dark fermentation in Synechocystis sp. PCC 6803, ACS Synthetic Biology, 9, 260-268
    # equally contributed authors
  80. Vavricka, CJ., Hasunuma, T., Kondo, A. (2020/01) (Review) Dynamic metabolomics for engineering biology: Accelerating learning cycles for bioproduction, Trends in Biotechnology, 38(1), 68-82
  81. Inokuma, K., Kurono, H., den Haan, R., van Zyl, W. H., Hasunuma, T.*, Kondo, A.* (2020/01) Novel strategy for anchorage position control of GPI-attached proteins in the yeast cell wall using different GPI-anchoring domains, Metabolic Engineering, 57, 110-117
  82. Aikawa, S., Nishida, A., Hasunuma, T.*, Chang, JS. Kondo, A. (2019/12) Short-term temporal metabolic behavior in halophilic cyanobacterium Synechococcus sp. strain PCC 7002 after salt shock, Metabolites, 9(12), 297
  83. Bamba, T., Yukawa, T., Guirimand, G., Inokuma, K., Sasaki, K., Hasunuma, T.*, Kondo, A. (2019/12) Production of 1,2,4-butanetriol from xylose by Saccharomyces cerevisiae through Fe metabolic engineering, Metabolic Engineering, 56: 17-27
  84. Huang, X., Bai, S., Liu, Z., Hasunuma, T., Kondo, A., Ho, SH. (2019/11) Fermentation of pigment-extracted microalgal residue using yeast cell-surface display: direct high-density ethanol production with competitive life cycle impacts, Green Chemistry, 22(1), 153-162
  85. Hasunuma, T.*, Takaki, A., Matsuda, M., Kato, Y., Vavricka, CJ., Kondo, A. (2019/10) Single-stage astaxanthin production enhances the nonmevalonate pathway and photosynthetic central metabolism in Synechococcus sp. PCC 7002, ACS Synthetic Biology, 8(12), 2701-2709
  86. Chen, JH., Kato, Y., Matsuda, M., Chen, CY., Nagarajan, D., Hasunuma, T., Kondo, A., Dong, CD., Lee, DJ., Chang, JS. (2019/10) A novel process for the mixotrophic production of lutein with Chlorella sorokiniana MB-1-M12 using aquaculture wastewater, Bioresource Technology, 290, 121786
  87. Guirimand, GGY., Bamba, T., Matsuda, M., Inokuma, K., Morita, K., Kitada, Y., Kobayashi, Y., Yukawa, T., Sasaki, K., Ogino, C., Hasunuma, T., Kondo, A. (2019/09) Combined cell surface display of β-D-glucosidase (BGL), maltose transporter (MAL11) and overexpression of cytosolic xylose reductase (XR) in Saccharomyces cerevisiae enhance cellobiose/xylose coutilization for xylitol bio-production from lignocellulosic biomass, Biotechnology Journal, 14(9): e1800704 (9月号表紙採用)
  88. Miyagishima, S., Era, A., Hasunuma, T., Matsuda, M., Hirooka, S., Sumiya, N., Kondo, A. Fujiwara, T. (2019/07) Day/night separation of oxygenic energy metabolism and nuclear DNA replication in the unicellular red alga Cyanidioschyzon merolae, mBio, 10(4): e00833-19
  89. Guirimand, G., Inokuma, K., Bamba, T., Matsuda, M., Morita, K., Sasaki, K., Ogino, C., Berrin, JG., Hasunuma, T., Kondo, A. (2019/05) Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass, Green Chemistry, 21, 1795-1808
  90. Vavricka, CJ., Yoshida, T., Kuriya, Y., Takahashi, S., Ogawa, T., Ono, F., Agari, K., Kiyota, H., Li, J., Ishii, J., Tsuge, K., Minami, H., Araki, M.*, Hasunuma, T.*, Kondo, A. (2019/05) Mechanism-based tuning of insect 3,4-dihydroxyphenylacetaldehyde synthase for synthetic bioproduction of benzylisoquinoline alkaloids, Nature Communications, 10, 2015
  91. Chen, JH., Chen, CY., Hasunuma, T., Kondo, A., Chang, CH. Ng, IS., Chang, JS. (2019/04) Enhancing lutein production with mixotrophic cultivation of Chlorella sorokiniana MB-1-M12 using different bioprocess operation strategies, Bioresource Technology, 278, 17-25
  92. Sakihama, Y., Hidese, R., Hasunuma, T.*, Kondo, A. (2019/03) Increased flux in acetyl-CoA synthetic pathway and TCA cycle of Kluyveromyces marxianus under respiratory conditions. Scientific Reports, 9(1): 5319
  93. Kato, Y., Fujihara, Y., Vavricka, CJ., Chang, JS., Hasunuma, T.*, Kondo, A. (2019/02) Light/dark cycling causes delayed lipid accumulation and increased photoperiod-based biomass yield by altering metabolic flux in oleaginous Chlamydomonas sp., Biotechnology for Biofuels, 12: 39
  94. Kobayashi, J., Sasaki, D., Bamba, T., Hasunuma, T., Kondo, A. (2019/02) Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae, Applied Microbiology and Biotechnology, 103(3), 1243-1254
  95. J. Amoah, Hasunuma, T., Ogino, C., Kondo, A. (2019/02) 5-Hydroxymethylfurfural production from salt-induced photoautotrophically cultivated Chlorella sorokiniana, Biochemical Engineering Journal, 142, 117-123
  96. Ito, Y., Watanabe, T., Aikawa, S., Nishi, T., Nishiyama, T., Nakamura, Y., Hasunuma, T., Okubo, Y., Ishii, J., Kondo, A. (2018/11) Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii, FEMS Yeast Research, 18(7), foy074
  97. Inokuma, K., Matsuda M., Sasaki, D., Hasunuma, T., Kondo, A. (2018/09) Widespread effect of N-acetyl-D-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis, Microbial Cell Factories, 17, 153
  98. Eguchi, Y., Makanae, K., Hasunuma, T., Ishibashi, Y., Kito, K., Moriya, H. (2018/08) Estimating the protein burden limit of yeast cells by measuring expression limits of glycolytic proteins, eLife, 7: e34595
  99. Hasunuma, T.*, Matsuda, M., Kato, Y., Vavricka, CJ., Kondo, A. (2018/07) Temperature enhanced succinate production concurrent with increased central metabolism turnover in the cyanobacterium Synechocystis sp. PCC 6803, Metabolic Engineering, 48, 109-120
  100. Nakamura, Y., Nishi, T., Noguchi, R., Ito, Y., Watanabe, T., Nishiyama, T., Aikawa, S., Hasunuma, T., Ishii, J., Okubo, Y., Kondo, A. (2018/07) A stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA, Applied and Environmental Microbiology, 84(15), e02882-17
  101. Tominaga, D., Kawaguchi, H., Hori, Y., Hasunuma, T., Ogino, C., Aburatani, S. (2018/05) Mathematical model for small size time series data of bacterial secondary metabolic pathways, Bioinformatics and Biology Insights, 12: 1-7
  102. Kawaguchi, H., Yoshihara, K., Hara, K. Y., Hasunuma, T., Ogino, C., Kondo, A. (2018/05) Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose, Microbial Cell Factories, 17: 76
  103. Nambu-Nishida, Y., Nishida, K., Hasunuma, T., Kondo, A. (2018/04) Genetic and physiological basis for antibody production by Kluyveromyces marxianus, AMB Express, 8: 56
  104. Bamba, T., Inokuma, K., Hasunuma, T., Kondo, A. (2018/03) Enhanced cell-surface display of a heterologous protein using SED1 anchoring system in SED1-disrupted Saccharomyces cerevisiae strain., Journal of Bioscience and Bioengineering, 125(3), 306-310
  105. Aikawa, S., Inokuma, K., Wakai, S., Sasaki, K., Ogino, C., Chang, JS, Hasunuma, T., Kondo, A. (2018/02) Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition, Biotechnology for Biofuels, 11: 50
  106. Nambu-Nishida, Y., Sakihama, Y., Ishii, J., Hasunuma, T., Kondo, A. (2018/01) Selection of yeast Saccharomyces cerevisiae promoters available for xylose cultivation and fermentation, Journal of Bioscience and Bioengineering, 125(1), 76-86
  107. Inokuma, K., Iwamoto, R., Bamba, T., Hasunuma, T., Kondo, A. (2017/12) Improvement of xylose fermentation ability under heat and acid co-stress in Saccharomyces cerevisiae using genome shuffling technique, Frontiers in Bioengineering and Biotechnology, 5, 81
  108. J. Amoah, Ho, SH., Hama, S., Yoshida, A., Nakanishi, A., Hasunuma, T., Ogino, C., Kondo, A. (2017/12) Conversion of Chlamydomonas sp. JSC4 lipids to biodiesel using Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cell as biocatalyst, Algal Research, 28, 16-23
  109. Kato, Y., Ho, SH., Vavricka, CJ., Chang, JS., Hasunuma, T.*, Kondo, A. (2017/12) Evolutionary engineering of salt-resistant Chlamydomonas sp. strains reveals salinity stress-activated starch-to-lipid biosynthesis switching, Bioresource Technology, 245(Part B), 1484-1490
  110. Mert, MJ., Rose, SH., la Grange, DC., Bamba, T., Hasunuma, T., Kondo, A., van Zyl, WH. (2017/10) Quantitative metabolomics of a xylose‑utilizing Saccharomyces cerevisiae strain expressing the Bacteroides thetaiotaomicron xylose isomerase on glucose and xylose, Journal of Industrial Microbiology and Biotechnology, 44(10), 1459-1470
  111. Nambu-Nishida, Y., Nishida, K., Hasunuma, T., Kondo, A. (2017/08) Development of a comprehensive set of tools for genome engineering in a cold- and thermo-tolerant Kluyveromyces marxianus yeast strain, Scientific Reports, 7(1): 8993
  112. Vavricka, CJ., Muto, C., Hasunuma, T., Kimura, Y., Araki, M., Wu, Y., Gao, GF., Ohrui, H., Izumi, M., Kiyota, H. (2017/08) Synthesis of sulfo-sialic acid analogues: potent neuraminidase inhibitors in regards to anomeric functionality, Scientific Reports, 7(1): 8239
  113. Liu, Z., Inokuma, K., Ho, SH., den Haan, R., van Zyl, W.H., Hasunuma, T., Kondo, A. (2017/06) Improvement of ethanol production from crystalline cellulose via optimizing cellulase ratios in cellulolytic Saccharomyces cerevisiae. Biotechnology and Bioengineering, 114(6), 1201-1207
  114. Ho, SH., Nakanishi, A., Kato, Y., Yamasaki, H., Chang, JS., Misawa, N., Hirose, Y., Minagawa, J., Hasunuma, T.*, Kondo, A. (2017/04) Dynamic metabolic profiling together with transcription analysis reveals salinity-induced starch-to-lipid biosynthesis in alga Chlamydomonas sp. JSC4, Scientific Reports, 4;7: 45471
  115. J. Amoah, Quayson, E., Hama, S., Yoshida, A., Hasunuma, T., Ogino, C., Kondo, A. (2017/03) Simultaneous conversion of free fatty acids and triglycerides to biodiesel by immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase., Biotechnology Journal, 12(3), 1600400
  116. Kobayashi, J., Sasaki, D., Hara, K.Y., Hasunuma, T., Kondo, A. (2017/03) Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae, Microbial Cell Factories, 16:44
  117. Hsu, H., Araki, M., Mochizuki, M., Hori, Y., Murata, M., Kahar, P., Yoshida, T., Hasunuma, T., Kondo, A. (2017/03) A systematic approach to time-series metabolite profiling and RNA-seq analysis of Chinese hamster ovary cell culture, Scientific Reports, 7:43518
  118. Takenaka, M., Kobayashi, T., Inokuma, K., Hasunuma, T., Maruyama, T., Ogino, C., Kondo, A. (2017/03) Mapping of endoglucanases displayed on yeast cell surface using atomic force microscopy., Colloids and Surfaces B: Biointerfaces, 151, 134-142
  119. Zhang. F., Wang, HW., Tominaga, K., Hayashi, M., Hasunuma, T., Kondo, A. (2017/02) Application of THz vibrational spectroscopy to molecular characterization and the theoretical fundamentals: an illustration using saccharide molecules., Chemistry - An Asian Journal, 12(3), 324-331
  120. Hsu, H., Hasunuma, T., Araki, M., Yoshida, T., Hori, Y., Murata, M., Kondo, A. (2017/02) Application of LC-MS/MS analysis for time-lapse amino acid metabolomics in CHO cell culture., Shimadzu Journal, 5 (1), 17-21
  121. Kono, T., Mehrotra, S., Endo, C., Kizu, N., Matsuda, M., Kimura, H., Mizohata, E., Inoue, T., Hasunuma, T., Yokota, A., Matsumura, H., Ashida, H. (2017/01) A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea., Nature Communications, 8 : 14007
  122. Tomita, Y., Yoshioka, K., Iijima, H., Nakashima, A., Iwata, O., Suzuki, K., Hasunuma, T., Kondo, A., Yokota, M., Osanai, T. (2016/12) Succinate and lactate production from Euglena gracilis during dark, anaerobic conditions., Frontiers in Microbiology, 7: 2050
  123. Inokuma, K., Hasunuma, T., Kondo, A. (2016/12) Ethanol production from N-acetyl-D-glucosamine by Scheffersomyces stipitis strains., AMB Express, 6, 83
  124. Kawaguchi, H., Hasunuma, T., Ogino, C., Kondo, A. (Review) (2016/12) Bioprocessing of bio-based chemicals produced from lignocellulosic feedstocks., Current Opinion in Biotechnology, 42: 30-39
  125. Ishigaki, M., Nakanishi, A., Hasunuma, T., Kondo, A., Morishima, T., Okuno, T., Ozaki, Y. (2016/11) High-speed scanning for the quantitative evaluation of glycogen concentration in bioethanol feedstock Synechocystis sp. PCC6803 by a near-infrared hyperspectral imaging system with a new near-infrared spectral camera., Applied Spectroscopy, 71, 3: 463-471
  126. Ohtani, M., Morisaki, K., Sawada, Y., Sano, R., Uy, L. T. A., Yamamoto, A., Kurata, T., Nakano, Y., Suzuki, S., Matsuda, M., Hasunuma, T., Hirai, M., Demura, T. (2016/11) Primary metabolism during biosynthesis of secondary wall polymers of protoxylem vessel elements., Plant Physiology,172(3), 1612-1624
  127. Ueno, M., Sae-Tang, P., Kusama, Y., Hihara, Y., Matsuda, M., Hasunuma, T., Nishiyama, Y. (2016/11) Moderate heat stress stimulates repair of photosystem II during photoinhibition in Synechocystis sp. PCC 6803., Plant and Cell Physiology, 57(11), 2417-2426
  128. Inokuma, K., Bamba, T., Ishii, J., Ito, Y., Hasunuma, T., Kondo, A. (2016/11) Enhanced cell-surface display and secretory production of cellulolytic enzymes with Saccharomyces cerevisiae Sed1 signal peptide., Biotechnology and Bioengineering, 113(11), 2358-2366
  129. Iijima, H., Shirai, T., Okamoto, M., Pinto, F., Tamagnini, P., Hasunuma, T., Kondo, A., Hirata, Y. M., Osanai, T. (2016/09) Metabolomics-based analysis revealing the alteration of primary carbon metabolism by the genetic manipulation of a hydrogenase HoxH in Synechocystis sp.PCC 6803., Algal Research,18, 305-313
  130. Liu, Z., Ho, S.H., Hasunuma, T., Chang, J.S., Ren, N., Kondo, A. (2016/09) Recent advances in yeast cell-surface display technologies for waste biorefineries., Bioresource Technology, 215,324-333
  131. Wang, Y., Chiu, SY., Ho, HS., Liu, Z., Hasunuma, T., Chang, TT., Chang, FK., Ren, QN., Kondo, A. (2016/08) Improving carbohydrate production of Chlorella sorokiniana NIES-2168 through semi-continuous process coupled with mixotrophic cultivation, Biotechnology Journal,11(8), 1072-1081
  132. Amoah, J., Ho, S.H., Hama, S., Yoshida, A., Nakanishi, A., Hasunuma, T., Ogino, C., Kondo, A. (2016/07) Lipase cocktail for efficient conversion of oils containing phospholipids to biodiesel, Bioresource Technology, 211, 224-230
  133. Hasunuma, T*., Matsuda, M., Kondo, A. (2016/05) Improved sugar-free succinate production by Synechocystis sp. PCC 6803 following identification of the limiting steps in glycogen catabolism., Metabolic Engineering Communications,3, 130-141
  134. Liu, Z., Ho, S.H. Sasaki, K., Haan, R., Inokuma, K., Ogino, C., van Zyl, W.H., Hasunuma, T., Kondo, A. (2016/04) Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production., Scientific Reports, 6: 24550
  135. Guirimand, G., Sasaki, K., Inokuma, K., Bamba T., Hasunuma, T., Kondo, A. (2016/04) Cell-surface engineering of Saccharomyces cerevisiae combined with membrane separation technology for xylitol production from rice straw hydrolysate, Applied Microbiology and Biotechnology, 100(8), 3477-3487
  136. Bamba, T., Hasunuma, T., Kondo, A. (2016/03) Disruption of PHO13 improves ethanol production via the xylose isomerase pathway., AMB Express, 6(1): 4
  137. Tsuge, Y., Kudou, M., Kawaguchi, H., Ishii, J., Hasunuma, T., Kondo, A. (2016/03) FudC, a protein primarily responsible for furfural detoxification in Corynebacterium glutamicum., Applied Microbiology and Biotechnology, 100(6), 2685-2692
  138. Hasunuma, T., Sakamoto, T., Kondo, A. (2016/01) Inverse metabolic engineering based on transient acclimation of yeast improves acidcontaining xylose fermentation and tolerance to formic and acetic acids, Applied Microbiology and Biotechnology, 100(2), 1027-1038
  139. Amoah, J., Ho, S.H., Hama, S., Yoshida, A., Nakanishi, A., Hasunuma, T., Ogino, C., Kondo, A. (2016/01) Converting oils high in phospholipids to biodiesel using immobilized Aspergillus oryzae wholecell biocatalysts expressing Fusarium heterosporum lipase., Biochemical Engineering Journal, 105, 10-15
  140. Hasunuma, T., Ishii, J., Kondo, A. (Review) (2015/12) Rational design and evolutional fine tuning of Saccharomyces cerevisiae for biomass breakdown, Current Opinion in Chemical Biology, 29: 1-9
  141. Hondo, S., Osanai, T., Matsuda, M., Hasunuma, T., Nakahira, Y., Chonan, S., Hasegawa, M., Asayama, M. (2015/11) Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria, Journal of Bioscience and Bioengineering, 120(5), 510-517
  142. Sumiya, N., Kawase, Y., Hayakawa, J., Matsuda, M., Nakamura, M., Era, A., Tanaka, K., Kondo, A., Hasunuma, T., Imamura, S., Miyagishima, S. (2015/10) Expression of cyanobacterial acyl-ACP reductase elevates the triacylglycerol level in the red alga Cyanidioschyzon merolae, Plant and Cell Physiology, 56(10), 1962-1980
  143. Liu, Z., Inokuma, K., Ho, S.H., den Haan, R., Hasunuma, T., van Zyl, W.H., Kondo, A. (2015/09) Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae, Biotechnology for Biofuels, 8: 162
  144. Sasaki, K., Okamoto, M., Shirai, T., Tsuge, Y., Teramura, H., Sasaki, D., Kawaguchi, H., Hasunuma, T., Ogino, C., Matsuda, F., Kikuchi, J., Kondo, A. (2015/06) Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content, Biotechnology for Biofuels, 8: 88
  145. Aikawa, S., Ho, S.H., Nakanishi, A., Chang, J.S., Hasunuma, T., Kondo, A. (Review) (2015/06) Improving polyglucan production in cyanobacteria and microalgae via cultivation design and metabolic engineering, Biotechnology Journal, 10(6), 886-898
  146. Sasaki, K., Tsuge, Y., Sasaki, D., Teramura, H., Inokuma,K., Hasunuma, T., Ogino C., Kondo, A. (2015/06) Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae, Bioresource Technology, 185, 263-268
  147. Ye, X., Morikawa, K., Ho, S.H., Nishida, K., Araki, M., Hasunuma, T., Hara, K.Y., Kondo, A. (2015/04) Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity, Microbial Cell Factories, 14: 56
  148. Inokuma, K., Ishii, J., Hara, K.Y., Mochizuki, M., Hasunuma, T., Kondo, A. (2015/04) Complete genome sequence of Kluyveromyces marxianus NBRC1777, a nonconventional thermotolerant yeast, Genome Announcements, 3(2), e00389-15
  149. Kawaguchi, H., Teramura, H., Uematsu, K., Hara, K.Y., Hasunuma, T., Hirano, K., Sazuka, T., Kitano, H., Tsuge, Y., Kahar, P., Niimi-Nakamura, S., Oinuma, K., Takaya, N., Kasuga, S., Ogino, C., Kondo, A. (2015/04) Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse, Bioresource Technology, 182:169-178
  150. Ho, S.H., Nakanishi, A., Ye, X., Chang, J.S., Chen, C.Y., Hasunuma, T.*, Kondo, A. (2015/03) Dynamic metabolic profiling of the marine microalga Chlamydomonas sp. JSC4 and enhancing its oil production by optimizing light intensity, Biotechnology for Biofuels, 8: 48
  151. Tsuge, Y., Hasunuma, T., Kondo, A. (Review) (2015/03) Recent advances in the metabolic engineering of Corynebacterium glutamicum for the production of lactate and succinate from renewable resources, Journal of Industrial Microbiology & Biotechnology, 42(3), 375-389
  152. Sakihama, Y., Hasunuma, T., Kondo, A. (2015/03) Improved ethanol production from xylose in the presence of acetic acid by the overexpression of the HAA1 gene in Saccharomyces cerevisiae, Journal of Bioscience and Bioengineering, 119(3), 297-302
  153. Tanaka, K., Iwasaki, K., Morimoto, T., Matsuse, T., Hasunuma, T., Takenaka, S., Chumsakul, O., Ishikawa, S., Ogasawara, N., Yoshida, K. (2015/02) Hyperphosphorylation of DegU cancels CcpA-dependent catabolite repression of rocG in Bacillus subtilis, BMC Microbiology, 15: 43
  154. Wan, C., Zhang, M., Fang, Q., Xiong, L., Zhao, X., Hasunuma, T., Bai, F., Kondo, A. (2015/02) The impact of zinc sulfate addition on the dynamic metabolic profiling of Saccharomyces cerevisiae subjected to long term acetic acid stress treatment and indentification of key metabolites involved in the antioxidant effect of zinc, Metallomics, 7(2), 322-332
  155. Inokuma, K., Yoshida, T., Ishii, J., Hasunuma, T., Kondo, A. (2015/02) Efficient co-displaying and artificial ratio control of α-amylase and glucoamylase on the yeast cell surface by using combinations of different anchoring domains, Applied Microbiology and Biotechnology, 99(4), 1655-1663
  156. Hasunuma, T.*, Matsuda, M., Senga, Y., Aikawa, S., Toyoshima, M., Shimakawa, G., Miyake C., Kondo, A. (2014/12) Overexpression of flv3 improves photosynthesis in the cyanobacterium Synechocystis sp. PCC6803 by enhancement of alternative electron flow, Biotechnology for Biofuels, 7: 493
  157. Hara, K.Y., Araki, M., Okai, N., Wakai, S., Hasunuma, T., Kondo A. (Review) (2014/12) Development of bio-based fine chemical production through synthetic bioengineering, Microbial Cell Factories, 13(1): 173
  158. Ismail, K.S.K., Sakamoto, T., Hasunuma, T., Zhao, X., Kondo, A. (2014/12) Zinc, magnesium and calcium ion supplementation confers tolerance to acetic acid stress in industrial Sacccharomyces cerevisiae utilizing xylose, Biotechnology Journal, 9(12), 1519-1525
  159. Ho, S.H., Ye, X., Hasunuma, T., Chang, J.S., Kondo, A. (Review) (2014/12) Perspectives on engineering strategies for improving biofuel production from microalgae - A critical review, Biotechnology Advances, 32, 1448-1459
  160. Hasunuma, T., Hori, Y., Sakamoto, T., Ochiai, M., Hatanaka, H., Kondo, A. (2014/10) Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicellulose, Microbial Cell Factories, 13(1): 145
  161. Tsuge, Y., Hori, Y., Kudou, M., Ishii, J., Hasunuma, T., Kondo, A. (2014/10) Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions, Applied Microbiology and Biotechnology, 98(20), 8675-8683
  162. Sasaki, K., Tsuge, Y., Sasaki, D., Hasunuma, T., Sakamoto, T., Sakihama, Y., Ogino, C., Kondo, A. (2014/10) Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae, Bioresource Technology, 169, 380-386
  163. Shimakawa, G., Hasunuma, T., Kondo, A., Matsuda, M., Makino, A., Miyake, C. (2014/08) Respiration accumulates Calvin cycle intermediates for the rapid start of photosynthesis in Synechocystis sp. PCC 6803, Bioscience, Biotechnology, and Biochemistry, 78(12), 1997-2007
  164. Kawaguchi, H., Uematsu, K., Ogino, C., Teramura, H., Niimi-Nakamura, S., Tsuge, Y., Hasunuma, T., Oinuma, K., Takaya, N., Kondo, A. (2014/07) Simultaneous saccharification and fermentation of kraft pulp by recombinant Escherichia coli for phenyllactic acid production, Biochemical Engineering Journal, 88, 188-194
  165. Morita, H., Hasunuma, T., Vassileva, M., Kondo, A., Tsenkova, R. (2014/06) A new screening method for recombinant Saccharomyces cerevisiae strains based on their xylose fermentation ability measured by near infrared spectroscopy, Analytical Methods, 6, 6628-6634
  166. Ho, S.H., Nakanishi, A., Ye, X., Chang, J.S., Hara, K.Y., Hasunuma, T.*, Kondo, A. (2014/06) Optimizing biodiesel production in marine Chlamydomonas sp. JSC4 through metabolic profiling and an innovative salinity-gradient strategy, Biotechnology for Biofuels, 7: 97
  167. Aikawa, S., Nishida, A., Ho, S.H., Chang, J.S., Hasunuma, T., Kondo, A. (2014/06) Glycogen production for biofuels by the euryhaline cyanobacteria Synechococcus sp. strain PCC 7002 from an oceanic environment, Biotechnology for Biofuels, 7: 88
  168. Joseph, A., Aikawa, S., Sasaki, K., Matsuda, F., Hasunuma, T., Kondo, A. (2014/03) Increased biomass production and glycogen accumulation in apcE gene deleted Synechocystis sp. PCC 6803., AMB Express, 4: 17
  169. Hasunuma, T., Ismail, K.S.K.,Nambu, Y., Kondo, A. (2014/02) Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural, Journal of Bioscience and Bioenginnering, 117(2), 165-169
  170. Inokuma, K., Hasunuma, T., Kondo, A. (2014/01) Efficient yeast cell-surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter, Biotechnology for Biofuels, 7(1): 8
  171. Joseph, A., Aikawa, S., Sasaki, K., Teramura, H., Hasunuma, T., Matsuda, F., Osanai, T., Hirai-Yokota, M., Kondo, A. (2014/01) Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803., FEBS Letters, 588(3), 466-471
  172. Nakanishi, A., Aikawa, S., Ho, S.H., Chen, C.Y., Chang, J.S., Hasunuma, T.*, Kondo, A. (2014/01) Development of lipid productivities under different CO2 conditions of marine microalgae Chlamydomonas sp. JSC4., Bioresource Technology, 152, 247-252
  173. Tsuge, Y., Tateno, T., Sasaki, K., Hasunuma, T.,Tanaka, T., Kondo, A. (2013/12) Direct production of organic acids from starch by cell surface engineered Corynebacterium glutamicumin anaerobic conditions, AMB Express, 3(1): 72
  174. Sasaki, K., Sasaki, D., Sakihama, Y., Teramura, H., Yamada, R., Hasunuma, T., Ogino, C., Kondo, A. (2013/11) Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration, Bioresource Technology, 147, 84-88
  175. Yamada, R., Hasunuma, T., Kondo, A. (Review) (2013/11) Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing, Biotechnology Advances, 31(6), 754-763
  176. Ismail, K.S.K., Sakamoto, T., Hasunuma, T., Kondo, A. (2013/09) Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production, Journal of Industrial Microbiology Biotechnology, 40(9), 1039-1050
  177. Kato, H., Matsuda, F., Yamada, R., Nagata, K., Shirai, T., Hasunuma, T., Kondo, A. (2013/09) Cocktail δ-integration of xylose assimilation genes for efficient ethanol production from xylose in Saccharomyces cerevisiae, Journal of Bioscience and Bioengineering, 116(3), 333-336
  178. Ho, SH., Kondo, A.,Hasunuma, T., Chang, JS. (2013/09) Engineering strategies for improving the CO2 fixation and carbohydrate productivity of Scenedesmus obliquus CNW-N used for bioethanol fermentation, Bioresource Technology, 143, 163-171
  179. Hasunuma, T.*, Kikuyama, F., Matsuda, M., Aikawa, S., Izumi, Y., Kondo, A. (2013/07) Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion, Journal of Experimental Botany, 64(10), 2943-2954
  180. Izumi, Y., Aikawa, S., Matsuda, F., Hasunuma, T., Kondo, A. (2013/07) Aqueous size-exclusion chromatographic method for the quantification of cyanobacterial native glycogen, Journal of Chromatography B, 930, 90-97
  181. Yamashita, Y., Yamaoka, M., Hasunuma, T., Ashida, H., Yoshida, K. (2013/05) Detection of orally administered inositol stereoisomers in mouse blood plasma and their effects on translocation of glucose transporter 4 in skeletal muscle cells, Journal of Agricultural and Food Chemistry, 61(20), 4850-4854
  182. Aikawa, S., Joseph, A., Yamada, R., Izumi, Y., Yamagishi, T., Matsuda, F., Kawai, H., Chang, JS., Hasunuma, T., Kondo, A. (2013/05) Direct conversion of Spirulina to ethanol without pretreatment or enzymatic hydrolysis processes, Energy & Environmental Science, 6(6), 1844-1849
  183. Hasunuma, T., Okazaki, F., Okai, N., Hara, K.Y., Ishii, J., Kondo, A. (Review) (2013/05) A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology, Bioresourse Technology, 135, 513-522
  184. Matano, Y., Hasunuma, T., Kondo, A. (2013/05) Cell recycle batch fermentation of high-solid lignocellulose using a recombinant cellulase-displaying yeast strain for high yield ethanol production in consolidated bioprocessing, Bioresourse Technology, 135, 403-409
  185. Ho, SH., Huang, SW., Chen, CY., Hasunuma, T., Kondo, A., Chang, JS. (2013/05) Bioethanol production, using carbohydrate-rich microalgae biomass as feedstock, Bioresource Technology, 135, 191-198
  186. Ho, SH., Huang, SW., Chen, CY., Hasunuma, T., Kondo, A. (2013/05) Characterization and optimization of carbohydrate production from an indigenous microalga Chlorella vulgaris FSP-E., Bioresource Technology, 135, 157-165
  187. Matano, Y., Hasunuma,T., Kondo, A. (2013/03) Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulase with a cell surface-engineered yeast strain, Applied Microbiology and Biotechnology, 97(5), 2231-2237
  188. Suga, H., Matsuda, F., Hasunuma,T., Ishii, J., Kondo, A. (2013/02) Implementation of a transhydrogenase-like shunt to counter redox imbalance during xylose fermentation in Saccharomyces cerevisiae, Applied Microbiology and Biotechnology, 97(4), 1669-1678
  189. Kondo, A., Ishii, J., Hara, K.Y., Hasunuma, T., Matsuda, F. (Review) (2013/01) Development of microbial cell factories for bio-refinery through synthetic bioengineering, Journal of Biotechnololgy, 163(2), 204-216
  190. Ishii, J., Yoshimura, K., Hasunuma, T., Kondo, A., (2013/01) Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway, Applied Microbiology and Biotechnology, 97(6), 2597-2607
  191. Sasaki, K., Matsuda, F., Hasunuma, T., Ogino, C., Urairi, M., Yoshida, K., Kondo, A. (2013/01) Ability of a perfluoropolymer membrane to tolerate by-products of ethanol fermentation broth from dilute acid-pretreated rice straw, Biocehmical Engineering Journal, 70, 135-139
  192. Ismail, K.S.K., Sakamoto, T., Hatanaka, Hasunuma, T., Kondo, A. (2013/01) Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose, Journal of Biotechnology, 163, 50-60
  193. Hasunuma, T., Kondo, A. (Review) (2012/11) Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering, Biotechnology Advances, 30(6), 1207-1218
  194. Hasunuma, T.*, Kondo, A. (Review) (2012/09) Consolidated bioprocessing and simultaneous saccharification and fermentation of lignocellulose to ethanol with thermotolerant yeast strains, Process Biochemistry, 47(9), 1287-1294
  195. Kato, H., Suyama, H., Yamada, R., Hasunuma, T., Kondo, A. (2012/06) Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains, Applied Microbiology and Biotechnology, 94(6), 1585-1592
  196. Kato, H., Izumi, Y., Hasunuma, T., Matsuda, F., Kondo, A. (2012/05) Widely targeted metabolic profiling analysis of yeast central metabolites, Journal of Bioscience and Bioengineering, 113(5), 665-673
  197. Fujitomi, K., Sanda, T., Hasunuma, T., Kondo, A., (2012/05) Deletion of the PHO13 gene in Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysate in the presence of acetic and formic acids, and furfural, Bioresource Technology, 111, 161-166
  198. Sakamoto, T., Hasunuma, T., Hori, Y., Yamada, R., Kondo, A. (2012/04) Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells, Journal of Biotechnology, 158(4), 203-210
  199. Matano, Y., Hasunuma, T., Kondo, A. (2012/03) Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass, Bioresource Technology, 108, 128-133
  200. Aikawa, S., Izumi, Y., Matsuda, F., Hasunuma, T., Chang, JS., Kondo, A. (2012/03) Synergistic enhancement of glycogen production in Arthrospira platensis by optimization of light intensity and nitrate supply, Bioresource Technology, 108, 211-215
  201. Sanda, T., Hasunuma, T., Matsuda, F., Kondo, A. (2011/09) Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids, Bioresourse Technology, 102, 7917-7924
  202. Matsuda, F., Yamasaki, M., Hasunuma, T., Ogino C., Kondo, A. (2011/08) Variation in biomass properties among rice diverse cultivars, Bioscience, Biotechnology, and Biochemistry, 75(8), 1603-1605
  203. Morita, H., Hasunuma, T., Vassileva M., Tsenkova, R., Kondo, A. (2011/06) Near infrared spectroscopy as high-throughput technology for screening of xylose-fermenting recombinant Saccharomyces cerevisiae strains, Analytical Chemistry, 83(11), 4023-4029
  204. Hasunuma, T., Sung, KM., Sanda, T., Yoshimura, K., Matsuda, F., Kondo, A. (2011/05) Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae, Applied Microbiology and Biotechnology, 90(3), 997-1004
  205. Hasunuma, T., Sanda, T., Yamada, R., Yoshimura, K., Ishii, J., Kondo, A. (2011/01) Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae, Microbial Cell Factories, 10(1): 2
  206. Kondo, A., Tanaka, T., Hasunuma, T., Ogino, C. (Review) (2010/11) Applications of yeast cell-surface display in bio-refinery, Recent Patent on Biotechnology, 4(3): 226-234
  207. Tanino, T., Hotta, A., Ito, T., Ishii, J., Yamada, R., Hasunuma, T., Ogino, C., Ohmura, N., Ohshima, T., Kondo, A. (2010/11) Construction of a xylose-metabolizing yeast by genome integration of xylose isomerase gene and investigation of the effect of xylitol on fermentation, Applied Microbiology and Biotechnology, 88, 1215-1221
  208. Yanase, S., Hasunuma, T., Yamada, R., Tanaka, T., Ogino, C., Fukuda, H., Kondo, A. (2010/09) Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes, Applied Microbiology and Biotechnology, 88(1), 381-388
  209. Saito, S., Hasunuma, T., Tanaka, T., Kondo, A. (2010/08) Co-fermentation of cellobiose and xylose using beta-glucosidase displaying diploid industrial yeast strain OC-2, Applied Microbiology and Biotechnology, 87(5), 1975-1982
  210. Yanase, S., Yamada, R., Kaneko, S., Noda, H., Hasunuma, T., Tanaka, T., Ogino, C., Fukuda, H., Kondo, A. (2010/05) Ethanol production from cellulosic materials using cellulase expressing yeast, Biotechnology Journal, 5, 449-455
  211. Hasunuma, T., Harada, K., Miyazawa, S., Kondo, A., Fukusaki, E., Miyake, C. (2010/02) Metabolic turnover analysis by a combination of in vivo C-13-labelling from (CO2)-C-13 and metabolic profiling with CE-MS/MS reveals rate-limiting steps of the C-3 photosynthetic pathway in Nicotiana tabacum leaves, Journal of Experimental Botany, 61(4), 1041-1051
  212. Hasunuma, T., Kondo, A., Miyake, C. (2009/03) Metabolic pathway engineering by plastid transformation is a powerful tool for production of compounds in higher plants, Plant Biotechnology, 26(1), 39-46
  213. Hasunuma, T., Miyazawa, S., Yoshimura, S., Shinzaki, Y., Tomizawa, K., Shindo, K., Choi, S., Misawa, N., Miyake, C. (2008/09) Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering, Plant Journal, 55(5), 857-868
  214. Hasunuma, T., Takeno, S., Hayashi, S., Sendai, M., Bamba, T., Yoshimura, S., Tomizawa, K., Fukusaki, E., Miyake, C. (2008/05) Overexpression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene in chloroplast contributes to increment of isoprenoid production, Journal of Bioscience and Bioengineering, 105(5), 518-526
  215. Shindo, K., Hasunuma, T., Asagi, E., Sano, A., Hotta, E., Minemura, N., Miyake, C., Maoka, T., Misawa, N. (2008/03) 4-Ketoantheraxanthin, a novel carotenoid produced by the combination of the bacterial enzyme β-carotene ketolase CrtW and endogenous carotenoid biosynthehtic enzymes in higher plants, Tetrahedron Letters, 49(20), 3294-3296
  216. Hasunuma, T., Fukusaki, E., Kobayashi, A. (2004/08) Expression of fungal pectin methylesterase in transgenic tobacco leads to alteration in cell wall metabolism and a dwarf phenotype, Journal of Biotechnology, 111(3), 241-251
  217. Hasunuma, T., Kuwabata, S., Fukusaki, E., Kobayashi, A. (2004/03) Real-time quantification of methanol in plants using a hybrid alcohol oxidase-peroxidase biosensor, Analytical Chemistry, 76(5), 1500-1506
  218. Hasunuma, T., Fukusaki, E., Kobayashi, A. (2003/12) Methanol production is enhanced by expression of an Aspergillus niger pectin methylesterase in tobacco cells, Journal of Biotechnology, 106(1), 45-52
  219. Fukusaki, E., Hasunuma, T., Kajiyama, S., Okazawa, A., Itoh, TJ., Kobayashi, A. (2001/11) SELEX for tubulin affords specific T-rich DNA aptamers. Systematic evolution of ligands by exponeential enrichment, Bioorganic & Medicinal Chemistry Letters, 11(33), 2927-2930

Reviews & Commentaries

  1. 番場崇弘, 棟方涼介, 矢﨑一史, 蓮沼誠久 プロポリス生理活性成分アルテピリンCの酵母による生産, バイオサイエンスとインダストリー(B&I), vol.83(3), 246-247 (2025/05)
  2. 蓮沼誠久, 第10章 循環型未来社会への取組み -6.バイオ燃料, 応用微生物学 第4版, 文永堂出版, 180-323 (2025/03)
  3. 蓮沼誠久, 第15章 材料/製造, 5.スマートセル・バイオものづくり, テクノロジー・ロードマップ 2025-2034 全産業編, 日経BP (2024/11)
  4. 蓮沼誠久, ppGpp に着目した細胞増殖制御による光合成的芳香族生産, BIO INDUSTRY, 41(10)通巻487号, 1-7 (2024/10)
  5. 松尾真紀子, 松本拓郎, 石塚大輔, 吉田彩乃, 城山英明, 蓮沼誠久, 立川雅司, 水無渉, バイオエコノミーの実現に向けたバイオものづくりの推進における課題と政策的検討を⾏う上での必須事項に関するフレームワーク, ワーキングペーパー, No.32 (2024/09)
  6. 蓮沼誠久, ppGppによるラン藻の窒素利用制御, 光アライアンス, 35(9), 18-21 (2024/09)
  7. 蓮沼誠久, 産業と行政　バイオものづくりに資するテンプレート酵素の探索と応用, Bioscience & Industry, 82(4), 432-434 (2024/07)
  8. 蓮沼誠久, 第8章 バイオマスの生物化学的変換　５節 代謝と遺伝子工学の利用, バイオマスの科学と技術 -未来を創るエネルギー-, コロナ社, 403-418 (2024/07)
  9. 蓮沼誠久，第11章 微生物によるカロテノイド高生産とメタボローム解析の応用, カロテノイドの科学 -基礎, 研究の新展開, 生理活性-, シーエムシー出版, 123-131 (2024/03)
  10. 蓮沼誠久, 14-6 スマートセルインダストリー, テクノロジー・ロードマップ 2024-2033 全産業編, 日経BP (2023/11)
  11. Kahar, P., Guirimand, G., Hasunuma, T., Ethanol Production by Recombinant CBP Yeasts, Handbook of Biorefinery Research and Technology, Springer, 1-22 (2023/10)
  12. 加藤悠一，田中謙也，蓮沼誠久，第5章 第14節 シアノバクテリアを利用した二酸化炭素からの有用物質生産技術，CO2の有効利用技術の開発，技術情報協会，293-300 (2023/7)
  13. 秀瀬涼太, 蓮沼誠久, 第5章 CO2からの有用物質生産 -2. 水素酸化細菌によるCO2吸収-, 微生物を活用した有用物質の製造技術, シーエムシー出版, 170-179 (2023/05)
  14. 蓮沼誠久, 第2章 高度なバイオものづくり用微生物を開発するための要素技術 -6. 微生物物質生産に資するオミクス技術-, 微生物を活用した有用物質の製造技術, シーエムシー出版, 73-80 (2023/05)
  15. 蓮沼誠久(監修), はじめに, 微生物を活用した有用物質の製造技術, シーエムシー出版 (2023/05)
  16. 田中謙也, 蓮沼誠久,「光合成が始まる瞬間の代謝機構」, 光合成研究，33，23-33 (2023/04)
  17. 蓮沼誠久, I編 生物資源利用の基礎：3. 物理・化学的変換技術の理論「3.3 バイオリファイナリー」, 119-128,
    Ⅱ編 生物資源の応用技術：2. エネルギー利用技術「2.4 バイオリファイナリー産物」, 229-237, 農業・食料生産分野におけるバイオマス利用工学 -循環型社会のための生物資源利用-, コロナ社 (2023/04)
  18. 蓮沼誠久, 特集 CO2から有用物質を生産し低炭素社会実現へ：AIと代謝工学を組み合わせ生産株開発を加速, JST news, 1, 10-11 (2023/01)
  19. 蓮沼誠久, これからの生物育種 -代謝工学による育種のこれから-, 日本生物工学会100年史, 第4章 生物工学のこれから, 58-60 (2022/10)
  20. 近藤昭彦, 蓮沼誠久, 秀瀬涼太, 育種技術 -突然変異から代謝工学へ-, 日本生物工学会100年史, 第3章 生物工学の研究100年, 29-31 (2022/10)
  21. 蓮沼誠久, 秀瀬涼太, 番場崇弘, [特集]「生物機能を利用したモノづくり」に貢献するプロセス強化　微生物の高速育種を実現するスマートセル創出プラットフォーム, 化学工学, 86(4), 157-160 (表紙採用) (2022/04)
  22. 猪熊健太郎, 蓮沼誠久, 進化する酵母細胞表層工学 -バイオインターフェイスの高度利用に向けた技術戦略-, オレオサイエンス, 22(3), 99-105 (2022/03)
  23. 猪熊健太郎，蓮沼誠久，クリーンエネルギーの技術と市場 2022　第9章　バイオマス燃料生産の技術動向，シーエムシー出版，84-91 (2022/02)
  24. 加藤悠一, 秀瀬涼太, 蓮沼誠久, 特集 藻類バイオマス利用のための新しい生物工学（後半）, 光合成メタボロミクスの物質生産への応用, 生物工学会誌, 99(9), 456-460 (2021/09)
  25. 蓮沼誠久, 新井宗仁, 特集 藻類バイオマス利用のための新しい生物工学, 生物工学会誌, 99(8), 403 (2021/08)
  26. 蓮沼誠久, 醸造の辞典, 第2章 醸造の科学, 最近の育種技術, 朝倉書店, 150-151 (2021/06)
  27. 蓮沼誠久, バイオリアクターのスケールアップと物質生産事例集, 第11章 スマートセルの動向と効率的創製に向けたAI活用, 第1節 国内におけるスマートセルの動向, 481-489, 技術情報協会 (2021/04)
  28. Kato, Y., Hasunuma, T. Metabolic Engineering for Carotenoid Production Using Eukaryotic Microalgae and Prokaryotic Cyanobacteria, Carotenoids: Biosynthetic and Biofunctional Approaches, Springer, 121-135 (2021/04)
  29. 蓮沼誠久, メタボロミクス 実践ガイド, 応用・展望編 Ⅰ. 応用研究　代謝工学分野へのメタボロミクスの応用, 羊土社, 302-304 (2021/04)
  30. 蓮沼誠久, 脱石油に向けたCO2資源化技術―化学・生物プロセスを中心に―, 第Ⅲ編　生物プロセス, 第2章　ラン藻によるバイオコハク酸生産, シーエムシー, 247-254 (2020/07)
  31. 番場崇弘, 湯川貴弘, 蓮沼誠久, 鉄代謝改変酵母による1,2,4-ブタントリオール生産, バイオサイエンスとインダストリー(B&I), vol.78(2), 114-115 (2020/03)
  32. 蓮沼誠久, ＜巻頭言＞スマートセルインダストリーの形成に向けたバイオ×デジタルの技術開発, 化学工学, Vol. 84(2), 51 (2020/02)
  33. 蓮沼誠久, Christopher J. Vavricka, 荒木通啓, 新たに設計した代謝経路を用いた大腸菌によるアルカロイド高生産, バイオサイエンスとインダストリー, Vol. 78(1), 32-33 (2020/01)
  34. 番場崇弘, 猪熊健太郎, Gregory Guirimand, 蓮沼誠久, 細胞表層提示酵母を利用したクラフトパルプからのキシリトール生産, バイオサイエンスとインダストリー(B&I), vol.77(5), 390-392 (2019/09)
  35. 蓮沼誠久, スマートセル開発に資するメタボロミクス技術, 特集 NEDOスマートセルプロジェクト, メタボローム解析技術開発, バイオサイエンスとインダストリー(B&I), vol.77(5), 410-411 (2019/09)
  36. 西村勇哉, 蓮沼誠久. 骨格筋研究を核とした筋スマート社会, 第3章 7. 組織内の分子を網羅的に測る技術, シーエムシー・リサーチ, 94-99 (2019/06)
  37. 蓮沼誠久, Branch Spirit, 生物工学会誌, Vol. 97(3), 150-151 (2019/03)
  38. 蓮沼誠久, チャンスを掴むための準備, バイオ系のキャリアデザイン, 生物工学会誌, vol96(10), 598-601 (2018/10)
  39. 蓮沼誠久, 田村具博, 近藤昭彦, 総論, スマートセルインダストリー -微生物細胞を用いた物質生産の展望-, 1-9, シーエムシー出版 (2018/06)
  40. 石井純, 西晶子, 北野美保, 中村朋美, 庄司信一郎, 秀瀬涼太, 蓮沼誠久, 近藤昭彦, 微生物を用いた物質生産とハイスループット微生物構築技術, スマートセルインダストリー -微生物細胞を用いた物質生産の展望-, 第1編 ハイスループット合成・分析・評価技術, 第2章 ハイスループット微生物構築・評価技術, 39-43, シーエムシー出版 (2018/06)
  41. 蓮沼誠久, スマートセル設計に資するメタボローム解析, スマートセルインダストリー -微生物細胞を用いた物質生産の展望-, 第1編 ハイスループット合成・分析・評価技術, 第3章 オミクス解析技術, 62-68, シーエムシー出版 (2018/06)
  42. 高久洋暁, 荒木秀雄, 小笠原渉, 田代康介, 蓮沼誠久, 油谷幸代, 矢追克郎, 第9章 油脂酵母による油脂発酵生産性改善へ向けた技術開発, スマートセルインダストリー -微生物細胞を用いた物質生産の展望-, 第3編 産業応用へのアプローチ, 206-214, シーエムシー出版 (2018/06)
  43. Inokuma, K., Hasunuma, T., Kondo, A. Emerging Areas in Bioengineering：Whole cell biocatalysts using enzymes displayed on yeast cell surface., Chang HN (Ed) Wiley-VCH, 81-92 (2018/01)
  44. 蓮沼誠久, バイオリファイナリー実現を加速する先端バイオ技術と情報技術の融合, アグリバイオ, 11月号, vol.1(12), 24-29 (2017/11)
  45. Hasunuma, T., Kondo, A. Industrial Biotechnoligy : Products and Processes, Chapter 5, Production of fuels and chemicals from biomass by integrated bioprocesses, Wiley, 161-186 (2016/11)
  46. 猪熊健太郎, 竹中武蔵, 荻野千秋, 蓮沼誠久, 近藤昭彦, 原子間力顕微鏡による酵母細胞表層セルラーゼの局在評価, 生物工学, 94, 698-700 (2016/11)
  47. 加藤悠一, 蓮沼誠久, 微細藻類を利用したバイオ燃料とカロテノイド類の生産, レーザー研究, 11，731-734 (2016/11)
  48. 猪熊健太郎, 蓮沼誠久, 近藤昭彦, バイオマスエネルギーの技術と市場, 第4章 次世代バイオエタノール製造技術, シーエムシー出版, 33-45 (2016/10)
  49. 蓮沼誠久, 応用微生物学, 第10章 低炭素化社会への取組み, バイオ燃料, 文永堂出版, 293-297 (2016/08)
  50. 日原由香子, 朝山宗彦, 蘆田弘樹, 天尾豊, 新井宗仁, 粟井光一郎, 得平茂樹, 小山内崇, 鞆達也, 成川礼, 蓮沼誠久, 増川一, 多彩な戦略で挑むシアノバクテリア由来の燃料生産 持続可能な第三世代バイオ燃料生産の最前線, 化学と生物, Vol.55, No.2, 88-977 (2017/02)
  51. 蓮沼誠久, 代謝プロファイリング法の微生物育種技術への応用, Journal of Environmental Biotechnology, Vol. 16, No. 1, 51–58, (2016)
  52. 蓮沼誠久, 石井純, 荻野千秋, 近藤昭彦, バイオリファイナリーの現状と展望, 化学と生物, Vol.53, No.10, 689-695 (2015/09)
  53. 石井純, 荒木通啓, 中津井雅彦, 崎濱由梨, 柘植陽太, 蓮沼誠久, 近藤昭彦, 合成生物工学によるモノづくり微生物のデザインに向けて, 生物工学会誌, 第93巻, 第9号, 523-526 (2015/09)
  54. 猪熊健太郎, 蓮沼誠久, 近藤昭彦, CBPによるバイオエタノール生産技術の開発, バイオインダストリー, Vol.32, No.5, 26-31 (2015/05)
  55. 蓮沼誠久, 代謝プロファイリングに基づく微生物育種技術の開発と応用, 生物工学会誌, 93(3), 122–129 (2015/03)
  56. 蓮沼誠久, 近藤昭彦, アーミング酵母による統合型バイオプロセスの開発, 日本エネルギー学会, Vol.93，No.7，580-585 (2014/07)
  57. Bisaria, V., Kondo, A. Bioprocessing of Renewable Resources to Commodity Bioproducts, Wiley (2014/04) Chapter 8, Ethanol production from yeasts, Hasunuma, T., Yamada, R., Kondo, A. 201-226
  58. 蓮沼誠久, 近藤昭彦, リグノセルロースからの高効率エタノール生産に向けた新規代謝改変酵母の創製, オレオサイエンス, Vol.14(3), 95-101 (2014/03)
  59. 化学便覧　応用化学編　28章　バイオマス利用技術　
    28.1 近藤昭彦,　28.2.3 荻野千秋,　28.5.1 蓮沼誠久・近藤昭彦,　28.5.3 原清敬・近藤昭彦,　28.6.2 岡井直子・近藤昭彦,　28.6.3 石井純・近藤昭彦,　28.6.6 山田亮祐・近藤昭彦, 丸善出版, 1615-1651 (2014/01)
  60. 蓮沼誠久, 近藤昭彦, 次世代パワートレイン開発と燃料技術, 第３編 第５章 3, 次世代バイオエタノール製造技術, シーエムシー出版, 184-193 (2013/11)
  61. 石井純, 蓮沼誠久, 松田史生, 近藤昭彦, バイオリファイナリー社会に向けた燃料・化学品生産, 安全工学,　Vol.52, No.4, 249-255 (2013/08)
  62. 石井純, 蓮沼誠久, 松田史生, 近藤昭彦, 革新的なものづくり実現のための「合成生物工学」, 生物工学会誌, 91(6), 314-318 (2013/06)
  63. 蓮沼誠久, 荻野千秋, 近藤昭彦, リサイクルバイオテクノロジーの最前線, 第I編 第1章, 1. 未来型CBP法によるバイオエタノール生産技術の展開, シーエムシー出版, 1-9 (2013/05)
  64. 蓮沼誠久, リサイクルバイオテクノロジーの最前線, 第I編 第1章, 8. 微細藻類・シアノバクテリアからのバイオエタノール生産, シーエムシー出版, 57-65 (2013/05)
  65. 崎濱由梨, 蓮沼誠久, 近藤昭彦, ひらく、ひらく「バイオの世界」, Part 4, バイオエタノールは地球にやさしいの？, 化学同人, 98-99 (2012/10)
  66. 岡井直子, 蓮沼誠久, 近藤昭彦, バイオリファイナリーとバイオプラスチック, ペトロテック, Vol. 35, No.10, 700-706 (2012/10)
  67. 蓮沼誠久, 近藤昭彦, 藻類ハンドブック, 第３章第５節３, システムバイオロジー技術, エヌ・ティー・エス, 535-540 (2012/07)
  68. 蓮沼誠久, 近藤昭彦, 合成生物工学によるバイオ燃料生産のための微生物細胞工場の創製, 生物工学会誌, 90(7), 386-391 (2012/07)
  69. 蓮沼誠久, 近藤昭彦, 微細藻類によるエネルギー生産と事業展望, 第17章, バイオリファイナリーの微細藻類への展開, シーエムシー出版, 137-143 (2012/07)
  70. Hasunuma, T., Matsuda, F., Kondo, A. System Metabolic Engineering, Chapter 10, Design of superior cell factories for a sustainable biorefinery by synthetic bioengineering, 329-348 (2012)
  71. 蓮沼誠久, 近藤昭彦, 合成生物工学の隆起, 第10章, 合成生物工学によるバイオ燃料生産のための微生物細胞工場の創製, シーエムシー出版, 150-158 (2012/04)
  72. 蓮沼誠久, 近藤昭彦, 微生物機能学, １１章, 微生物を利用して有限な化石資源から脱却する, 三共出版, 119-133 (2012/03)
  73. 山田亮祐, 蓮沼誠久, 近藤昭彦, バイオマス分解酵素研究の最前線, 28章, セルラーゼ細胞表層提示酵母を用いたバイオマス変換, シーエムシー出版, 265-271 (2012/03)
  74. 蓮沼誠久, 近藤昭彦, バイオマス分解酵素研究の最前線, 序章2, バイオマス分解酵素研究の新たな展開, シーエムシー出版, 6-9 (2012/03)
  75. 吉田健一, 蓮沼誠久, 有用希少イノシトールのバイオアベイラビリティーとバイオコンバージョン生産, 生物工学会誌, 89(10), 585-588 (2011/10)
  76. 蓮沼誠久, 荻野千秋, 近藤昭彦, ＣＢＰ用スーパー酵母を用いたバイオエタノール生産技術の開発, ブレインテクノニュース, No.144, 17-22 (2011/10)
  77. 蓮沼誠久, 近藤昭彦, バイオマスからの効率的なバイオ燃料の生産技術, ケミカルエンジニヤリング, 56(9), 5-12 (2011/09)
  78. 蓮沼誠久, 藍川晋平, 和泉自泰, 近藤昭彦, 微細藻類によるバイオリファイナリー, 生物工学会誌, 89(4), 181-183 (2011/04)
  79. 近藤昭彦, 荻野千秋, 蓮沼誠久, 田中勉, 中島一紀, エコバイオリファイナリー, 第６章, バイオエネルギーと新プラットフォーム形成, シーエムシー出版, 82-91 (2010/12)
  80. 近藤昭彦, 荻野千秋, 蓮沼誠久, 田中勉, 中島一紀, バイオマスからのバイオ燃料生産に向けた戦略, バイオインダストリー, Vol 11, 6-12 (2010/11)
  81. 近藤昭彦, 荻野千秋, 蓮沼誠久, 田中勉, 中島一紀, 次世代燃料・化成品原料製造に向けたバイオリファイナリー戦略, 生物工学, 88(7), 333-335 (2010/07)
  82. 荻野千秋, 蓮沼誠久, 近藤昭彦, グリーンバイオケミストリーの最前線, 第Ⅱ編第1章, セルロース・デンプン, シーエムシー出版, 23-28 (2010/04)
  83. Hasunuma, T., Kondo, A., Miyake, C. Methods in Molecular Biology, Vol.643, Plant Secondary Metabolism Engineering, Metabolic engineering by plastid transformation as a strategy to modulate isoprenoid yield in plants., Humana Press, 213-228 (2010/02)
  84. 山田亮祐, 蓮沼誠久, 近藤昭彦, 次世代バイオエタノール生産の技術革新と事業展開, 第10章7, アーミング技術を応用した新規セルロース分解システムの構築, フロンティア出版, 282-286 (2010/01)
  85. 蓮沼誠久, 山田亮祐, 近藤昭彦, 次世代バイオエタノール生産の技術革新と事業展開, 第７章4, アーミング酵母を用いた統合プロセスによるバイオエタノール生産, フロンティア出版, 164-170 (2010/01)
  86. 蓮沼誠久, 原田和生, 福崎英一郎, 第二世代バイオ燃料の開発と応用展開, 第6章3, 代謝ターンオーバー解析とバイオマス改変への応用, シーエムシー出版, 121-130 (2009/04)
  87. 蓮沼誠久, 原田和生, 三宅親弘, 福崎英一郎, 光合成研究法, 3章1-b 安定同位体標識を利用した動的代謝プロファイリング, 北海道大学低温科学研究所・日本光合成研究会 共編, 169-174 (2009/03)
  88. 蓮沼誠久, 近藤昭彦, 水環境の今と未来 藻類と植物のできること, 第8章 バイオマスからの燃料, 化学品生産-水生バイオマス利用への期待-, 神戸大学水圏光合成生物研究グループ編, 109-120 (2009/03)
  89. 蓮沼誠久, 岡野憲司, 舘野俊博, 近藤昭彦, バイオマスからの化学品生産を目指したバイオプロセスの開発, ケミカルエンジニアリング, 54(3), 24-30 (2009/03)

Invited lecture

  1. 蓮沼誠久，藻類によるバイオものづくりへの挑戦，日本藻類学会 第49回大会 公開企業セッション「微細藻類を活用した価値創造と産業利用の現状」，沖縄，2025.3.23
  2. 蓮沼誠久，バイオものづくりに資するスマートセル創出プラットフォームの開発，大阪大学ナノテク社会人教育プログラム，オンライン，2025.2.7
  3. Hasunuma, T. Identification of highly active promiscuous enzymes using information analysis and high-throughput experimental systems, Asian Synthetic Biology Association (ASBA) 2025, Singapore, 2025.1.7
  4. 蓮沼誠久，バイオものづくりの現在地 ―最新技術と今後の課題―，Stockmark社，オンライン，2024.11.21
  5. 蓮沼誠久，藻類によるバイオものづくりの現状と将来，第22回 産業界におけるカーボンニュートラル研究会，大阪，2024.11.19
  6. Hasunuma, T. Data-driven engineering biology for advanced biomanufacturing, UK-Japan Roundtable Discussion, London, 2024.10.18
  7. Hasunuma, T. Kobe Biofoundry, Global Biofoundry Alliance (GBA) Meeting 2024, Daejeon, 2024.10.10
  8. Hasunuma, T. Advanced cellulosic biorefinery and digital engineering biology, 15th Globe Industrial Microbiology Congress & Metabolic Engineering Summit 2024, Shanghai, 2024.9.22
  9. 蓮沼誠久，次世代のバイオものづくりに貢献する微生物，国際微生物デイ2024，神戸，2024.9.16
  10. Hasunuma, T. Data-driven synthetic biology for biomanufacturing, Northeast Asian Symposium 2024, Seoul, 2024.9.11
  11. 蓮沼誠久，バイオものづくりに資するスマートセルの開発，島津製作所イブニングフォーラム2024，幕張，2024.9.5
  12. Hasunuma, T. Metabolomics-based photosynthetic metabolic engineering, European Congress on Biotechnology, Rotterdam, 2024.7.3
  13. 蓮沼誠久，バイオものづくりに資するスマートセル創出プラットフォームの開発，関西バイオものづくりオンライン月例発表会2024，オンライン，2024.6.28
  14. 蓮沼誠久，藻類によるバイオものづくりへの挑戦，第30回 光合成学会若手の会セミナー，神戸，2024.6.1
  15. Hasunuma, T. Data-driven synthetic biology for biomanufacturing, KIChE Spring Meeting, Jeju, 2024.4.25
  16. Hasunuma, T. Metabolomics-based engineering biology for microbial bio-production, KIChE Spring Meeting, Jeju, 2024.4.25
  17. 蓮沼誠久, データ駆動型の合成生物学による生産株開発の加速, 化学工学会バイオプロセス講演会, オンライン, 2024.3.15
  18. 蓮沼誠久, 微細藻類・シアノバクテリアを利用したCO2からの直接物質生産, 日本微生物資源学会公開シンポジウム, 東京, 2024.3.12
  19. Hasunuma, T. Digitalization of biotechnology to accelerate DBTL, Asian Synthetic Biology Association (ASBA) 2023, Awaji, Japan, 2023.12.16
  20. 蓮沼誠久，微生物を活用した液体燃料・汎用化学品・機能性物質の製造技術，CMCリサーチウェビナー，オンライン，2023.12.7
  21. 蓮沼誠久，微細藻類によるバイオものづくり、SDGsへの貢献，日本油化学会 油脂実践講座2023，オンライン，2023.12.5
  22. 蓮沼誠久，バイオとデジタルの融合によるスマートセル創出プラットフォームの開発，日本放線菌学会学術講演会，東京，2023.11.9
  23. 蓮沼誠久，藻類バイオものづくりに資する藻株プラットフォーム開発，BioJapan 2023出展者セミナー「“藻類農業” が地球と次世代の持続可能な成長を結びつける」，横浜，2023.10.13
  24. 蓮沼誠久，バイオとデジタルの技術融合による先端バイオ工学研究プラットフォーム確立に向けた挑戦，第75回 日本生物工学会大会シンポジウム「森も木も見る微生物研究：連携による集積知，名古屋，2023.9.4
  25. Hasunuma, T. High-throughput analytics and automation for engineering biology metrology and standards, Engineering Biology Metrics and Technical Standards for the Global Bioeconomy: Asia and Australia Workshop, Singapore, 2023.8.30
  26. 蓮沼誠久，データ駆動型の合成生物学による生産株開発の加速，JST/OPERA第13回「機能性バイオ」ミニシンポ バイオものづくりと日本の展望，千葉，2023.7.31
  27. Hasunuma, T. Dynamic metabolomics approach and engineering biology for microbial carotenoid production, 19th International Symposium on Carotenoid, Toyama, Japan, 2023.7.13
  28. 蓮沼誠久，データ駆動型のバイオものづくりプラットフォームと質量分析，日本質量分析学会　第71回 質量分析総合討論会 株式会社島津製作所 ランチョンセミナー，大阪，2023.5.15
  29. Hasunuma, T. Advanced cellulosic biorefinery and digital engineering biology, 14th AFOB Regional Symposium (ARS 2023), Faridabad, India, 2023.4.28
  30. 蓮沼誠久，微生物を活用した液体燃料・汎用化学品・機能性物質の製造技術，CMCリサーチウェビナー，オンライン，2023.4.14
  31. Hasunuma, T. Metabolomics-based engineering biology for microbial bio-production, 2023 Korea Metabolomics Society Annual Meeting, Seoul, Korea, 2023.4.6
  32. 蓮沼誠久，スマートセルを創出する世界最先端バイオ工学プラットフォーム，関西バイオものづくりフォーラム，大阪，2023.3.8
  33. 蓮沼誠久，データ駆動型のバイオものづくりプラットフォーム，CMXワークショップ Emergence Conference，神戸，2023.1.12
  34. 蓮沼誠久，微生物を利用した有用物質生産，情報機構セミナー，オンライン，2022.12.16
  35. Hasunuma, T. Development of microalgae highly producing oil from CO2, QST Takasaki Lab Open Seminar, Takasaki, 2022.11.28
  36. Hasunuma, T. Data-driven engineering biology for bio-production, Joint SBJ meeting with Indonesia, Philippine and Thailand, Osaka, 2022.10.21
  37. 蓮沼誠久，バイオDXによるスマートセル開発，第74回日本生物工学会大会シンポジウム「バイオエコノミーに資するバイオ×デジタル融合型の次世代研究プラットフォームの開発」，大阪，2022.10.20
  38. 蓮沼誠久，微細藻類を用いたモノづくりの可能性，BioJapan 2022出展者セミナー，横浜，2022.10.13
  39. 蓮沼誠久，スマートセル開発に資するメタボローム解析技術，BioJapan 2022主催者セミナー，横浜，2022.10.12
  40. 蓮沼誠久，日本の先端バイオとカロテノイド研究，2022年度カロテノイド若手の会，兵庫，2022.9.16
  41. 蓮沼誠久，シアノバクテリアや微細藻類を利用したCO2からの直接物質生産，第39回植物バイオテクノロジー学会大会シンポジウム「藻類の多様性研究の持続的社会への貢献」，大阪，2022.9.13
  42. 蓮沼誠久，次世代型バイオ分析により様々な微生物を活用する生物工学の未来，JASISトピックスセミナー「日本発の発酵工学の歴史から発展する未来展望」，千葉，2022.9.8
  43. 蓮沼誠久，微生物を利用した有用物質生産技術の開発動向と今後の展望，Science & Technology社セミナー，オンライン，2022.8.9
  44. 蓮沼誠久，バイオ・デジタル融合に資する「ハイスループットかつ高精度な代謝解析技術」，OEB機能性食品分科会セミナー，オンライン，2022.7.20
  45. 蓮沼誠久，バイオDXによるスマートセル開発とバイオ生産プロセスへの応用，有機合成化学協会研究部会第9回勉強会，オンライン，2022.7.15
  46. 蓮沼誠久，DX導入によるバイオ実験の効率化と自律型実験システム構築への展開，技術情報協会「実験自動化・自律化」セミナー，オンライン，2022.6.10
  47. 蓮沼誠久，バイオDXによるスマートセル開発，化学工学会第87年会 異分野合同セッション データ駆動型研究開発の先端動向，神戸，2022.3.17
  48. 蓮沼誠久，バイオ×デジタルの技術融合によるスマートセル創出と低炭素化技術への応⽤，第3回 東京理科⼤学総合研究院合成⽣物学研究部⾨シンポジウム，2022.3.11
  49. 蓮沼誠久，バイオ燃料の現状と将来―化石燃料代替の輸送用燃料からバイオものづくりまで―，地球環境と産業化研究会（SGEIS）／第9回「脱炭素と省エネビジネス」勉強会，2021.12.8
  50. 蓮沼誠久，バイオとデジタルの技術融合によるスマートセルの創出，2021年電気化学秋季大会シンポジウム／S4.生命科学と電気化学，2021.9.8
  51. 蓮沼誠久，スマートセル開発に資する代謝解析と自動化技術，日本化学会第101春季年会 イノベーション共創プログラム T3D スマートセルインダストリーという未来，2021.3.22
  52. 蓮沼誠久，ポストコロナ時代の産学連携について考える，レーザー学会学術講演会第41回年次大会シンポジウム，2021.1.18
  53. 蓮沼誠久，バイオプロセスにおける膜利用の現状と将来展望，第一回先端膜工学研究推進機構特定テーマフォーラム～医薬・バイオプロセスにおける膜利用の現状と将来展望～，2020.12.22
  54. Hasunuma, T. Metabolomics-based engineering biology for microbial bio-production with sustainability, 2nd ASBA Webinar, 2020.12.11
  55. 蓮沼誠久，ハイスループットプラットフォームの開発による微生物スマートセルの構築，CBI学会2020年大会，2020.10.27
  56. 蓮沼誠久，加藤悠一，微細藻類を利用した物質生産プロセスへのLEDの応用，JPC関西特別報告会，2020.10.1
  57. 蓮沼誠久，メタボローム解析の自動化に向けた挑戦，Laboratory Automation勉強会，2020.8.29
  58. 蓮沼誠久，バイオ×デジタルの技術融合による有用微生物「スマートセル」開発への挑戦，質量分析インフォマティクス研究会　第５回ワークショップ, 2020.8.7
  59. 蓮沼誠久，スマートセルインダストリーに資するメタボローム解析技術の開発と応用，農芸化学会関西支部第511回講演会，神戸，2019.12.7
  60. Hasunuma, T. Metabolomics-based engineering for bio-production, Asian Synthetic Biology Association (ASBA) 2019, Chengdu, China, 2019.10.27
  61. Hasunuma, T. Metabolomics-based engineering for smart cell development, Metabolic Engineering Summit 2019, Tianjin, China, 2019.10.22
  62. 蓮沼誠久, バイオ×デジタルによるスマートセル開発への挑戦, BioJapan 2019 JST ALCAシンポジウム, 2019.10.10
  63. Hasunuma, T. Smart cell development based on high throughput technology, advanced analysis and computational science for bio-production in microorganisms, The 14th Asian Congress on Biotechnology (ACB), Tamsui Fisherman’s Warf, Taiwan, 2019.7.3
  64. 蓮沼誠久, スマートセル創出プラットフォーム構築へ向けた挑戦, 日本生物工学会 第6回SBJシンポジウム「超スマート社会（Society 5.0）の実現に向けた『生物工学』の挑戦!!」, 大阪, 2019.5.24
  65. 蓮沼誠久, スマートセル創出プラットフォームの構築と実証に向けて, 関西スマートセルフォーラム2018「スマートセルを巡る最新技術動向」, 大阪, 2019.1.30
  66. Hasunuma, T. Platform technologies for development of smart cell, 1st Asia Synthetic Biology Association, Korea, 2018.11.23
  67. Hasunuma, T. Development of "smart cell" construction platform for next-generation microbial breeding, Kyoto Univ. Clock Tower Centennial Hall, Kyoto, 2018.11.19-21
  68. 蓮沼誠久, 次世代型微生物育種に資するスマートセル創出プラットフォームの開発, BioJapan2018「バイオ×デジタルで加速するスマートセルインダストリー/我が国の新しいバイオ政策とスマートセルインダストリーの実現に向けて」, 横浜, 2018.10.10
  69. 蓮沼誠久, 先端バイオ工学研究センターについて, iBioK第8回合成生物工学シンポジウム, 神戸, 2018.9.26
  70. 蓮沼誠久, スマートセル創出プラットフォームに資するメタボローム解析技術の開発, 第70回日本生物工学会大会シンポジウム「スマートセル開発のためのバイオ技術とデジタル技術の革新と融合」, 大阪, 2018.9.7
  71. 蓮沼誠久, 酵母の代謝解析の最新知見と化粧品原料への応用, 第70回日本生物工学会 SK-IIランチョンセミナー, 大阪, 2018.9.7
  72. 蓮沼誠久, 動的メタボロミクスの開発とスマートセルインダストリーへの展開, 第13回アジレントメタボロミクスセミナー2018, 東京, 2018.7.25
  73. 蓮沼誠久, スマートセル創出プラットフォームに資するハイスループット微生物構築・評価技術の開発, モノづくり日本会議 第20回新産業技術促進技術検討会「バイオで切り拓くモノづくりの新潮流～スマートセル創出プラットフォームの構築と実証～」, 東京, 2018.7.19
  74. 蓮沼誠久，スマートセルインダストリーの創出に資する微生物育種プラットフォームの開発，新化学技術推進協会（JACI）ライフサイエンス技術部会・反応分科会講演会講演会「スマートセルインダストリーに関する研究開発動向」，東京，2018.2.26
  75. 蓮沼誠久，藻類オイル生産の実用化に向けた代謝メカニズム解析の重要性，微細藻燃料開発推進協議会（JMAF）開催 シンポジウム，東京，2018.1.29
  76. 蓮沼誠久，Engineering Biologyによるバイオリファイナリーの構築とスマートセルインダストリーへの展開, 静岡大学グリーン科学研究所 第4回シンポジウム，浜松，2017.11.9
  77. 蓮沼誠久，スマートセルを創出する合成バイオプラットフォームの開発と応用への挑戦，iBioK第7回 合成生物工学シンポジウム，神戸，2017.8.3
  78. 蓮沼誠久，進化した細胞表層工学によるバイオマス変換プロセスの開発と機能性物質生産への新展開，新化学技術推進協会（JACI）エネルギー・資源技術部会 バイオマス分科会 講演会「バイオマス資源変換触媒の研究動向」，東京，2017.4.21
  79. 蓮沼誠久，バイオリファイナリーの構築に資する微生物細胞工場の創製，第21回　関西大学　先端科学技術シンポジウム/地域資源の高度利用を図るバイオリファイナリーの基盤形成とその実用化，大阪，2017.1.20
  80. 蓮沼誠久，バイオリファイナリーの構築に資する微生物細胞工場の創製，第118回　触媒討論会，岩手，2016.9.21
  81. Hasunuma, T. Bio-based fuel and chemical production based on dynamic metabolic profiling of cyanobacteria and microalgae, 4th Asia-Oceania Algae Innovation Summit, AOAIS2016, Wuhan, China, 2016.9.19
  82. Hasunuma, T. Development of cell factories of microalgae and cyanobacteria based on metabolic analysis, KMB2016, Annual meeting and international symposium, Daejeon, Korea, 2016.6.23
  83. 蓮沼誠久，代謝プロファイリング法の微生物育種への応用，環境バイオテクノロジー学会2016年度大会，広島，2016.6.14
  84. 蓮沼誠久，動的代謝プロファイリング技術の開発とバイオリファイナリーへの展開，第340回細胞工学研究会後援会／第220回遺伝子機能解析部門セミナー，島根，2016.3.15
  85. Hasunuma, T. Dynamic metabolic analysis of microorganisms for the development of bio-based chemical production, 7th international symposium of iBioK, Kobe, 2016.1.27
  86. 蓮沼誠久，藻類を利用したバイオエタノール，バイオベース化学品生産，平成27年度新資源生物変換研究会シンポジウム「次世代のバイオ技術を切り開く日本の強み」，一般財団法人バイオインダストリー協会，東京，2016.1.25
  87. Hasunuma, T. Development of cell factories of microalgae and cyanobacteria based on metabolic analysis, New Horizon in Biotechnology 2015, Trivandrum, India, 2015.11.23
  88. 蓮沼誠久，メタボロームのターンオーバー解析技術（代謝プロファイリング）の開発と物質生産への応用，iBioK第5回　合成生物工学シンポジウム，神戸2015.8.5
  89. 蓮沼誠久，マルチオミクス解析に基づく合理的細胞育種技術の開発，ＨＭＴセミナー，大阪，2015.7.10
  90. Hasunuma, T. Biofuel production from microalgae and cyanobacteria based on metabolic profiling, KMB2015, 42nd annual meeting and international symposium, Gyeongju, Korea, 2015.6.25
  91. Hasunuma, T. Development of microbial cell factories based on metabolic engineering, 2015 KSBB Spring Meeting and International Symposium, Yeosu, Korea, 2015.4.15
  92. Hasunuma, T. Development of dynamic metabolic profiling of cyanobacteria and microalgae, 3rd Asia-Oceania Algae Innovation Summit, Daejeon, Korea, 2014.11.18
  93. 蓮沼誠久，合成生物工学によるバイオ燃料生産に資する微生物の創製，広島大学バイオマスイブニングセミナー，広島，2014.12.17
  94. 蓮沼誠久，微細藻類の代謝系解析に基づく次世代バイオ燃料生産への挑戦，ＮＰＯ法人近畿バイオインダストリー振興会議　バイオマス研究会　第29回研究会，大阪，2014.11.28
  95. 蓮沼誠久，代謝システム解析に基づく合理的細胞育種技術の開発，iBioK第4回　合成生物工学シンポジウム，神戸，2014.11.25
  96. Hasunuma, T. Development of dynamic metabolic profiling of microalgae, 5th Asian symposium on innovative bio-production and biorefinery in Taiwan, Tainan, Taiwan, 2014.11.10
  97. 蓮沼誠久，代謝システム解析に基づく細胞育種とバイオ医薬品生産への応用，第30回バイオロジカルズ（タンパク医薬）製造技術研究会セミナー，東京，2014.11.7
  98. Hasunuma, T. Biodiesel production in marine microalgae through metabolic profiling and an innovative salinity-gradient strategy, Korean Marine Biology and Biotechnology International Symposium, Inha, Korea, 2014.10.16
  99. 蓮沼誠久，オミクス解析のバイオリファイナリー，バイオ医薬品生産への応用，アジレント・メタボロミクスデイズ 2014，東京，2014.10.2
  100. 蓮沼誠久，バイオリファイナリーに向けた微生物の開発と代謝系解析の応用，日本植物学会第78回大会・株式会社島津製作所ランチョンセミナー，東京，2014.9.12
  101. 蓮沼誠久，代謝プロファイリングに基づく微生物育種技術の開発と応用，第66回日本生物工学会大会／第50回生物工学奨励賞（斎藤賞）受賞講演，札幌，2014.9.10
  102. 蓮沼誠久，近藤昭彦，新たな挑戦イノベーティブ・バイオプロダクション，INCHEM TOKYO2013産学官マッチングフォーラム，東京，2013．10.30
  103. 蓮沼誠久，in vivo 13C標識法によるラン藻動的代謝プロファイリング，第28回つくば藻類・プロティストフォーラム，筑波，2013.10.28
  104. Hasunuma, T. Biofuel production from halophilic algae, i-BioB2013, Beijing, China, 2013.10.21
  105. Hasunuma, T. Development of microalgal cell factories based on sysmtems biology approach, 1st Korea-Japan Microalgae Symposium, Daejeon, Korea, 2013.10.11
  106. 蓮沼誠久，メタボローム解析による次世代バイオ燃料生産のための微細藻代謝改変戦略の導出，微細藻類研究会2013，岡崎，2013.6.13
  107. 蓮沼誠久，近藤昭彦，神戸大学におけるバイオリファイナリーの進展，化学工学会第78年会／化学工学会関西支部　開発型企業連携研究会，大阪，2013.3.17
  108. Hasunuma, T. Biorefinery from microalgae through fermentation and metabolic engineering, SBJ 64th Annual Meeting, Kobe, 2012.10.26
  109. 蓮沼誠久，バイオリファイナリー構築に資する微生物細胞工場の創製，アジレントメタボロミクスセミナー，東京，2012.7.6
  110. Hasunuma, T. Microbial engineering for production of biofuels and biorefinery products, 4th A-IMBN Annual Conference, Thailand, 2012.3.1
  111. Hasunuma, T., Kondo, A. Production of bio-ethanol from microalgae through cell surface engineering, 5th International Algae Congress, Berlin, Germany, 2011.12.2
  112. 蓮沼誠久，補助食品・スピルリナのメタボローム解析，LECOジャパン・食品分析セミナー，東京，2011.11.16
  113. 蓮沼誠久，シンセティックバイオエンジニアリングによるバイオエタノール生産酵母の育種，日本生物工学会　第99回醗酵学懇話会，神戸，2011.8.4
  114. Kondo, A., Ogino, C., Hasunuma, T. Development of microbial cell factories for biofuel production from biomass, BioEco 2011, Tianjin, China, 2011.6.27
  115. 蓮沼誠久，合成生物工学によるバイオ燃料生産のための微生物細胞工場の創製，JBA新資源生物変換研究会主催シンポジウム「バイオマスの今、そして未来」，神戸，2011.6.17
  116. Hasunuma, T. Production of bio-ethanol from lignocellulosic materials through the engineering of yeast cell surface and metabolic pathway, 4th Japan-Korea Biomass Symposium, Kyoto, 2010.11.5
  117. Hasunuma, T. A synthetic biology approach for efficient production of cellulosic ethanol in Saccharomyces cerevisiae, 2nd international symposium of innovative bioproduction Kobe, Kobe, 2010.1.21
  118. 蓮沼誠久，アーミング酵母を用いた統合プロセスによるバイオエタノール生産，日本化学会関東支部講演会「バイオマス変換最前線」，東京，2009.11.6
  119. Hasunuma, T., Kondo, A. Effective biofuel production process from biomass reseources by whole-cell biocatalyst, Airbus meeting, Tokyo, 2009.11.5