Members

BioProduction Engineering Lab Members (Eng. Biol. Res. C) Shumpei Asamizu

Associate ProfessorShumpei Asamizu

Education and Experience

Apr, 2001 - Mar, 2005
College of Technology, Toyama Prefectural University
Apr, 2005 - Mar, 2010
Graduate School of Enginieering, Toyama Prefectural University
Apr, 2008 - Mar, 2010
Japan Society for the Promotion of Science Resercher
Apr, 2010 - Mar, 2013
Postdoc, Department of Pharmaceutical Sciences, Oregon State University
Apr, 2013 - Apr, 2013
Project Researcher, Graduate School of Agricultural and Life Sciences, The University of Tokyo
May, 2013 - Apr, 2019
Project Assistant Professor, Graduate School of Agricultural and Life Sciences, The University of Tokyo
May, 2019 - Mar, 2023
Project Lecturer, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Apr, 2023 - May, 2023
Project Researcher, Faculty of Science, Department of Life Science, Gakushuin University
Jun, 2023 - Present
Project Associate Professor, Engineering Biology Research Center, Kobe University

Awards

  • May 2023, Hot Topics Award (13th) at Annual Meeting of JSBBA 2023
    Studies on arsenic-related secondary metabolism in actinobacteria.
  • May 2022, Hot Topics Award (12th) at Annual Meeting of JSBBA 2022
    Biological significance of the phage tail-like nanostructures produced by soil bacteria.
  • May 2021, Hot Topics Award (11th) at Annual Meeting of JSBBA 2021
    Analysis of fatty acyl chain biosynthesis in goadvionins.
  • May 2018, Hot Topics Award (9th) at Annual Meeting of JSBBA 2018
    Analysis of lanthipeptide biosynthetic enzymes responsible for formation of novel labionen structure.
  • Sep 2016, Hamada award (The Society for Actinomycetes Japan)
  • May 2016, JSBBA Award for Young Scientists (Japan Society for Bioscience, Biotechnology, and Agrochemistry)

Publications

  1. Hoshino, S., Ijichi, S., Asamizu, S. and Onaka, H. (2023/8): Insights into arsenic secondary metabolism in actinomycetes from the structure and biosynthesis of bisenarsan. J. Am. Chem. Soc.
  2. Ishikawa, F.*, Tsukumo, N., Morishita, E., Asamizu, S., Kusuhara, S., Marumoto, S., Takashima, K., Onaka, H. and Tanabe, G.* (2023/7): Activity-based protein profiling-guided biosynthetic diversification of nonribosomal peptides. Chem Commun (Camb).
  3. Ijichi, S., Hoshino, S., Asamizu, S.* and Onaka, H.* (2023/6): SolS-catalyzed sulfoxidation of labionin to solabionin drives antibacterial activity of solabiomycins. Bioorg. Med. Chem. Lett. 89: 129323
  4. Nagakubo, T.*, Asamizu, S., Yamamoto, T., Kato, M., Nishiyama, T., Toyofuku, M., Nomura, N. and Onaka, H.* (2023/6): Intracellular phage tail-like nanostructures affect susceptibility of Streptomyces lividans to osmotic stress. mSphere. 8(3): e0011423
  5. Lei, Y., Asamizu, S., Ishizuka, T, Onaka, H. (2023/02) Regulation of multidrug efflux pumps by TetR family transcriptional repressor negatively affects secondary metabolism in Streptomyces coelicolor A3(2), Applied and Environmental Microbiology, e0182222
  6. Tsunoda, T., Asamizu, S., Mahmud, T. (2022/10) Biochemical characterization of GacI, a bifunctional glycosyltransferase–phosphatase enzyme involved in acarbose biosynthesis in Streptomyces glaucescens GLA.O, Biochemistry, 61(22): 2628-2635
  7. Asamizu, S., Ijichi, S., Hoshino, S., Jo, H., Takahashi, H., Itoh, Y., Matsumoto, S., Onaka, H. (2022/09) Stable isotope-guided metabolomics reveals polar-functionalized fatty-acylated RiPPs from Streptomyces, ACS Chemical Biology, 17(10): 2936-2944
  8. Asamizu, S.*, Cahya Pramana, AA., Kawai, SJ., Arakawa, Y., Onaka, H. (2022/09) Comparative metabolomics reveals a bifunctional antibacterial conjugate from combined-culture of Streptomyces hygroscopicus HOK021 and Tsukamurella pulmonis TP-B0596, ACS Chemical Biology, 17(9): 2664-2672
  9. Yanagisawa, M., Asamizu, S., Satoh, K., Oono, Y., Onaka, H. (2022/07) Effects of carbon ion beam-induced mutagenesis for the screening of RED production-deficient mutants of Streptomyces coelicolor JCM4020, PLoS One, 17(7): e0270379
  10. Kato, M., Asamizu, S., Onaka H. (2022) Intimate relationships among actinomycetes and mycolic acid-containing bacteria, Scientific Reports, 12(1): 7222
  11. Asai, Y., Hiratsuka, T., Ueda, M., Kawamura, Y., Asamizu, S., Onaka, H., Arioka, M., Nishimura, S., Yoshida, M. (2022/01) Differential biosynthesis and roles of two ferrichrome-type siderophores, ASP2397/AS2488053 and ferricrocin, in Acremonium persicinum, ACS Chemical Biology, 17(1): 207-216
  12. Hikima, A., Asamizu, S., Onaka, H., Zhang, H., Tomoda, H., Koyama, N. (2022) Kimidinomycin, a new antibiotic against Mycobacterium avium complex, produced by Streptomyces sp. KKTA-0263, The Journal of Antibiotics, 75(2): 72-76
  13. Nagakubo, T., Yamamoto, T., Asamizu, S., Phage tail-like nanostructures affect microbial interactions between Streptomyces and fungi, Scientific Reports, 11(1): 20116
  14. Kozakai, R., Ono, T., Hoshino, S., Takahashi, H., Katsuyama, Y., Sugai, Y., Ozaki, T., Teramoto, K., Teramoto, K., Tanaka, K., Abe, I., Asamizu, S., and Onaka H.(2020/9): Acyltransferase that catalyses the condensation of polyketide and peptide moieties of goadvionin hybrid lipopeptides. Nat. Chem. 12(9): 869-877
  15. Vaario, LM., Asamizu, S., Sariala, T., Matsushita, N., Onaka, H., Xia, Y., Kurokochi, H., Morinaga, S., Huang, J., Zhang, S. and Lian, C. (2020/4): Bioactive properties of streptomyces may affect the dominance of Tricholoma matsutake in shiro. Symbiosis. 81: 1-13, 2020
  16. Vinogradov, AA., Shimomura, M., Goto, Y., Ozaki, T., Asamizu, S., Sugai, Y., Suga, H., Onaka, H. (2020/05) Minimal lactazole scaffold for in vitro thiopeptide bioengineering. Nat. Commun. 11(1): 2272
  17. Sugiyama, R., Nakatani, T., Nishimura, S., Takenaka, K., Ozaki, T., Asamizu, S., Onaka, H., Kakeya, H. (2019/9) Chemical interactions of cryptic actinomycete metabolite 5-alkyl-1,2,3,4-tetrahydroquinolines through aggregate formation. Angew. Chem. Int. Ed. Engl. 58(38)strong>: 13486-13491
  18. Ozaki, T., Sugiyama, R., Shimomura, M., Nishimura, S., Asamizu, S., Katsuyama, Y., Kakeya, H., Onaka, H. (2019/2) Identification of the common biosynthetic gene cluster for both antimicrobial streptoaminals and antifungal 5-alkyl-1,2,3,4-tetrahydroquinolines. Org. Biomol. Chem. 17(9): 2370-2378
  19. Hoshino, S., Wong, CP., Ozeki, M., Zhang, H., Hayashi, F., Awakawa, T., Asamizu, S., Onaka,H., Abe,I. (2018/7) Umezawamides, new bioactive polycyclic tetramate macrolactams isolated from a combined-culture of Umezawaea sp. and mycolic acid-containing bacterium.J. Antibiot. (Tokyo). 71(7): 653-657
  20. Hoshino, S., Okada, M., Awakawa, T., Asamizu, S., Onaka,H., Abe,I. (2017/9) Mycolic acid containing bacterium stimulates tandem cyclization of polyene macrolactam in a lake sediment derived rare actinomycete. Org. Lett. 19(18): 4992-4995
  21. Osborn, AR., Kean, KM., Alseud, KM., Almabruk, KH., Asamizu, S., Lee, JA., Karplus, PA., Mahmud, T., (2017/2) Evolution and distribution of C7-cyclitol synthases in prokaryotes and eukaryotes. ACS Chem. Biol. 12(4): 979-988
  22. Ozaki, T., Yamashita, K., Goto, Y., Shimomura, M., Hayashi, S., Asamizu, S., Sugai, Y., Ikeda, H., Suga, H., Onaka, H. (2017) Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogs expressed in vivo. Nat. Commun. 8: 14207
  23. Sugiyama, R., Nishimura, S., Ozaki, T.,Asamizu, S., Onaka, H., Kakeya, H. (2016/7) Discovery and total synthesis of streptoaminals: antimicrobial [5,5] -spirohemiaminals from the combined-culture of Streptomyces nigrescens and Tsukamurella pulmonis. Angew. Chem. Int. Ed. Engl. 55(35): 10278-10282
  24. Ozaki, T., Kurokawa, Y., Hayashi, S., Oku, N., Asamizu, S., Igarashi, Y., Onaka, H. (2016/2) Insights into the biosynthesis of dehydroalanines in goadsporin. Chembiochem. 17(3): 218-223
  25. Asamizu, S., Ozaki, T., Teramoto, K., Satoh, K., Onaka, H. (2015/6) Killing of mycolic acid-containing bacteria aborted induction of antibiotic production by Streptomyces in combined-culture. PloS One, 10(11): e0142372
  26. Osborn, AR., Almabruk, KH., Holzwarth, G., Asamizu, S., LaDu, J., Kean, KM., Karplus, PA., Tanguay, RL., Bakalinsky, AT., Mahmud, T. (2015/5) De novo synthesis of a sunscreen compound in vertebrates. eLIFE. 4: e05919
  27. Sugiyama, R., Nishimura, S., Ozaki, T., Asamizu, S., Onaka, H., Kakeya, H. (2015/4) 5-Alkyl-1,2,3,4-tetrahydroquinolines (5aTHQs), new membrane-interacting lipophilic metabolites, produced by combined-culture of Streptomyces nigrescens and Tsukamurella pulmonis. Org. Lett. 17(8): 1918-1921
  28. Onaka, H., Ozaki, T., Mori, Y., Izawa, M., Hayashi, S. and Asamizu, S. (2015/9) : Mycolic-acid-containing bacteria activate heterologous secondary metabolite expression in Streptomyces lividans. J. Antibiot. (Tokyo). 68(9): 594-597, 2015.
  29. Kean, KM., Codding, SJ., Asamizu, S., Mahmud, T.and Karplus PA.* (2014/5) : Structure of a sedoheptulose 7-phosphate cyclase: ValA from Streptomyces hygroscopicus. Biochemistry. 53(26): 4250-4260. (Correction: 53(26): 4316-4316)
  30. Hayashi S., Ozaki, T., Asamizu, S., Ikeda, H., Ōmura, S., Oku, N., Igarashi, Y., Tomoda, H. and Onaka, H.* (2014/5): Genome mining reveals six genes, a minimum gene set for the biosynthesis of 32-membered macrocyclic thiopeptides. Chem. Biol. 21(5): 679-688
  31. Haginaka, K., Asamizu, S., Ozaki, T., Igarashi, Y., Furumai, T.and Onaka, H.* (2014/4): Genetic approaches to generate hyper-producing strains of goadsporin: the relationships between productivity and gene duplication in secondary metabolite biosynthesis. Biosci. Biotechnol. Biochem. 78(3): 394-399
  32. Asamizu, S., Abugreen, M. and Mahmud, T.* (2013/8): Comparative metabolomic analysis of an alternative biosynthetic pathway to pseudosugars in Actinosynnema mirum DSM 43827. Chembiochem. 14(13): 1548-1551
  33. Asamizu, S., Hirano, S., Onaka,H., Koshino, H., Shiro, Y.and Nagano, S.* (2012/10): Coupling reaction of indolepyruvic acid by StaD and its product: Implications for biosynthesis of indolocarbazole and violacein. Chembiochem. 13(17): 2495-2500
  34. Cavalier, MC., Yim, TS., Asamizu, S., Neau, D., Almabruk, KH., Mahmud, T. and Lee, YH.* (2012): Mechanistic insights into validoxylamine A 7’-phosphate synthesis by VldE using the structure of the entire product complex. PloS One. 7(9): e44934
  35. Almabruk, KH., Asamizu, S., Chang, A., Varghese, SG. and Mahmud, T.* (2012/9): The α-ketoglutarate/FeII-dependent dioxygenase VldW is responsible for the formation of validamycin B. Chembiochem. 13(15): 2209-2211
  36. Asamizu, S., Xie, P., Brumsted, CJ., Flatt, PM. and Mahmud, T.*(2012/7): Evolutionary divergence of sedoheptulose 7-phosphate cyclases leads to several distinct cyclic products. J. Am. Chem. Soc. 134(29): 12219-12229
  37. Asamizu, S., Shiro, Y., Igarashi, Y., Nagano, S. and Onaka, H.* (2011/11): Characterization and functional modification of StaC and RebC, which are involved in the pyrrole oxidation of indolocarbazole biosynthesis. Biosci. Biotechnol. Biochem. 75(11): 2184-2193
  38. Asamizu, S., Yang, J., Almabruk, KH. and Mahmud, T.* (2011/8): Pseudoglycosyltransferase catalyzes nonglycosidic C-N coupling in validamycin A biosynthesis. J. Am. Chem. Soc. 133(31): 12124-12135
  39. Wang, Y., Chen, H., Makino, M., Shiro, Y., Nagano, S., Asamizu, S., Onaka, H. and Shiak,S.* (2009/5): Theoretical and experimental studies of the conversion of chromopyrrolic acid to an antitumor derivative by cytochromo P450 StaP: The catalytic role of water molecules. J. Am. Chem. Soc. 131(19): 6748-6762
  40. Hirano, S., Asamizu, S., Onaka,H.*, Shiro, Y. and Nagano, S.* (2008/3): Crystal structure of VioE, a key player in the construction of the molecular skeleton of violacein. J. Biol. Chem. 283(10): 6459-6466
  41. Makino, M., Sugimoto, H, Shiro, Y., Asamizu, S., Onaka, H.* and Nagano, S* (2007/7): Crystal structures and catalytic mechanism of cytochrome P450 StaP that produces the indolocarbazole skeleton. Proc. Natl. Acad. Sci. USA. 104(28): 11591-11596
  42. Asamizu, S., Kato, Y., Igarashi, Y. and Onaka, H.* (2007/4): VioE, a prodeoxyviolacein synthase involved in violacein biosynthesis, is responsible for intramolecular indole rearrangement. Tetrahedron Lett. 48(16): 2923-2926
  43. Asamizu, S., Kato, Y., Igarashi, Y., Furumai, T. and Onaka, H.* (2006/1): Direct formation of chromopyrrolic acid from indole-3-pyruvic acid by StaD, a novel hemoprotein in indolocarbazole biosynthesis. Tetrahedron Lett. 47(4): 473-475
  44. Onaka, H., Asamizu, S., Igarashi, Y., Yoshida, R. and Furumai, T. (2005/9): Cytochrome P450 homolog is responsible for C-N bond formation between aglycone and deoxysugar in the staurosporine biosynthesis of Streptomyces sp. TP-A0274. Biosci. Biotechnol. Biochem. 69(9): 1753-1759

Reviews & Commentaries

  1. 尾仲宏康，浅水俊平：物理的接触が介在する放線菌二次代謝誘導，環境バイオテクノロジー学会誌20: 13-22, (2020)
  2. 尾仲宏康，丸山潤一，浅水俊平，黒岩真弓，北本勝ひこ，山田雅人，五島徹也，赤尾健：富山県産大麦から分離した酵母菌株「とやま産まれの酵母」の清酒醸造特性，J. Brew. Soc. Japan. 114: 1-9, (2019/10)
  3. 浅水俊平，尾﨑太郎，尾仲宏康：ミコール酸含有細菌死菌体は放線菌の二次代謝を誘導しない，IFO Res. Commun. 32: 73-86, (2018)
  4. 浅水俊平，尾﨑太郎，菅井佳宣，尾仲宏康：ミコール酸含有細菌に対する放線菌二次代謝応答機構の網羅的転写解析，IFO Res. Commun. 32: 87-94, (2018/12)
  5. 浅水俊平，菅井佳宣，尾﨑太郎，尾仲宏康：重イオンビーム変異導入法によるTsukamurella pulmonisへの赤色色素非生産応答性Streptomyces coelicolor突然変異株の解析，IFO Res. Commun. 32: 95-104, (2018/12)
  6. 尾﨑太郎，浅水俊平，尾仲宏康：複合培養を用いた新規アルカロイドの同定と生合成に関する研究，IFO Res. Commun. 32: 105-115, (2018/12)
  7. 浅水俊平，菅井佳宣，尾仲宏康：放線菌複合培養液からの抗生物質-シデロフォア連結化合物の発見，IFO Re.s Commun. 32: 117-127, (2018/12)
  8. 尾﨑太郎，菅井佳宣，浅水俊平，尾仲宏康：ゴードスポリン作用機構の解析，IFO Res. Commun. 32: 129-139, (2018/12)
  9. 尾﨑太郎，菅井佳宣，浅水俊平，尾仲宏康：In vitro再構成系を用いたゴードスポリン生合成酵素の解析と合理的な類縁体設計，IFO Res. Commun. 32: 141-154, (2018/12)
  10. 林昌平，尾﨑太郎，浅水俊平，尾仲宏康：ゲノム探索による32員環新規チオペプチドラクタゾールの発見，IFO Res. Commun. 32: 73-86, (2018/12)
  11. 菅井佳宣，尾﨑太郎，浅水俊平，尾仲宏康：新規ラビオネン構造の形成に関与するランチペプチド合成酵素の解析，IFO Res. Commun. 32: 167-177, (2018)
  12. 浅水俊平，尾仲宏康：異属細菌により誘導される放線菌の特殊代謝，生物工学会誌96: 457-460, (2018)
  13. Asamizu, S.: Biosynthesis of nitrogen-containing natural products, C7N aminocyclitols and bis-indoles, from actinomycetes. Biosci. Biotechnol. Biochem. 81: 871-881, (2017)
  14. Asamizu, S.: Exploiting the potential of biosynthesis of natural products by actinomycetes: bacterial interaction-driven natural product discovery and biosynthetic machinery. Actinomycetologica, 31: S30-S40, (2017)
  15. 浅水俊平：もう無視できない天然物資源，生物工学会誌93: 489, (2015/8)

Books

  1. Onaka, H., Asamizu, S., Metabolite induction via microorganism symbiosis and co-culturing: a potential way to enhance chemical diversity. Comprehensive Natural Products III. Liu, HW. and Begley, TP. eds. Elsevier, 7: 487-501 (ISBN 978-0-08-102691-5)（分担執筆） (2020/07/22)